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The map label for this gene is phoB [H]

Identifier: 157960131

GI number: 157960131

Start: 338086

End: 339288

Strand: Reverse

Name: phoB [H]

Synonym: Spea_0302

Alternate gene names: 157960131

Gene position: 339288-338086 (Counterclockwise)

Preceding gene: 157960132

Following gene: 157960130

Centisome position: 6.56

GC content: 45.22

Gene sequence:

>1203_bases
TTGGGCAAAGAAATCCTACTCGTTGAAGATAACAAGGTCATGCAACTCTTGATGACTAAAATGCTGTCTTCAAGAGGCTA
TCAAGTCGTTACCGCATTTGATGGAGTTGACGCCATTGCCCAACTTGAAAAACATCCCTCAATCCAATTAGTCCTCAGTG
ATTGGATGATGCCGAAGTTAAACGGCATCGAGCTCTGTTATCACTTAAAATCCTCCGACTATGGCCGCTACATCTTCTTT
GTTTTGCTATCAGGGCGCGATGACGAGGAGTCGATTGTTGAAGGTATTAATGCCGGAGCCGATGACTTTGTCGCCAAAGA
AACCAACATCAATGAACTCGATGCTCGCCTTAAAGCAGGCTTTAGAAGCCTAGAATTGCATAATCAGTTGATGTCCAAAA
ACATCGAACTCGATCGCGCTTATGCCACAATCCGCCAAGATCTAGACTCAGCTAACGCCCTTATCCGCCGTATGCTACCA
AGTCAGACTGAGTTTCTAGGAGTCGAGCTACATTACACTCATATTCCCAGCGCACAAATAGGCGGCGATATGCTGGGATG
CATGCAACTAGATGAAGAGCATGTTGCCATTTATCTATTTGACGTCGCCGGTCATGGGGTTGCCTCTGCACTGATGTCAT
TCGCTATTCAGCAAAGCATTAGCTCCAACTCTGGAACCCATTCACATGTGAAGGGGAAGAAAGACACCGATCCATTTTAT
ATGCTGAGAGATCCAAGTGAAGTGCTGGCAAGGCTCAATCAAAGCTATTTGAGCCAAGACAATAACAATCTGTATTTCAC
TATGGTTTATGCGGTGTTAAATATTAAGAGCGGCCTCTTAAGCTACGCCAGTGCCGGACATCCACCAATGATCTGGCTAC
AAACGCAATCACAAACGGCGCATTTTATCGAGCAGGACAGCTTTGTGGCAGGCATGTTCGACTTTGCCAGTTATCAAACC
GAACTGGTTCAGCTCGAACAAGGCGACCGCATCTTACTGTATTCAGACGGCATCACCGAAGCAGAGTCAGCTAGCAAAGA
GCAATACTCCGAACAAGGCTTAAAAAGCCTGGTGCAAGAGTTGGCTTCTCACCCTACAAAATCACAAACTGAAACCATAG
TAAATGCAGTTAAAGACTGGTGCGGTATAGAGCAATTTGATGACGACGTGAGTTTATTAGCCTTTGAATGGAAAGGTAAT
TAA

Upstream 100 bases:

>100_bases
GTATTCTAAAATCACTACTAGACTTCTGAAAGTAAAAGGACTTATTAAAAATCCTAGCGTTTTTCGCTCGGTAATAAGCT
ACGGCACGGAGGCTGAAAGT

Downstream 100 bases:

>100_bases
AAAAAGGAAATATCATGCAATTTGAAATAATTGAAAAGGGTCAGTATACCGTTATTCAAGTCAATGAAGCTCGATTTGAT
GCGAGGCTAGCCCCGTCTTT

Product: response regulator receiver modulated serine phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MGKEILLVEDNKVMQLLMTKMLSSRGYQVVTAFDGVDAIAQLEKHPSIQLVLSDWMMPKLNGIELCYHLKSSDYGRYIFF
VLLSGRDDEESIVEGINAGADDFVAKETNINELDARLKAGFRSLELHNQLMSKNIELDRAYATIRQDLDSANALIRRMLP
SQTEFLGVELHYTHIPSAQIGGDMLGCMQLDEEHVAIYLFDVAGHGVASALMSFAIQQSISSNSGTHSHVKGKKDTDPFY
MLRDPSEVLARLNQSYLSQDNNNLYFTMVYAVLNIKSGLLSYASAGHPPMIWLQTQSQTAHFIEQDSFVAGMFDFASYQT
ELVQLEQGDRILLYSDGITEAESASKEQYSEQGLKSLVQELASHPTKSQTETIVNAVKDWCGIEQFDDDVSLLAFEWKGN

Sequences:

>Translated_400_residues
MGKEILLVEDNKVMQLLMTKMLSSRGYQVVTAFDGVDAIAQLEKHPSIQLVLSDWMMPKLNGIELCYHLKSSDYGRYIFF
VLLSGRDDEESIVEGINAGADDFVAKETNINELDARLKAGFRSLELHNQLMSKNIELDRAYATIRQDLDSANALIRRMLP
SQTEFLGVELHYTHIPSAQIGGDMLGCMQLDEEHVAIYLFDVAGHGVASALMSFAIQQSISSNSGTHSHVKGKKDTDPFY
MLRDPSEVLARLNQSYLSQDNNNLYFTMVYAVLNIKSGLLSYASAGHPPMIWLQTQSQTAHFIEQDSFVAGMFDFASYQT
ELVQLEQGDRILLYSDGITEAESASKEQYSEQGLKSLVQELASHPTKSQTETIVNAVKDWCGIEQFDDDVSLLAFEWKGN
>Mature_399_residues
GKEILLVEDNKVMQLLMTKMLSSRGYQVVTAFDGVDAIAQLEKHPSIQLVLSDWMMPKLNGIELCYHLKSSDYGRYIFFV
LLSGRDDEESIVEGINAGADDFVAKETNINELDARLKAGFRSLELHNQLMSKNIELDRAYATIRQDLDSANALIRRMLPS
QTEFLGVELHYTHIPSAQIGGDMLGCMQLDEEHVAIYLFDVAGHGVASALMSFAIQQSISSNSGTHSHVKGKKDTDPFYM
LRDPSEVLARLNQSYLSQDNNNLYFTMVYAVLNIKSGLLSYASAGHPPMIWLQTQSQTAHFIEQDSFVAGMFDFASYQTE
LVQLEQGDRILLYSDGITEAESASKEQYSEQGLKSLVQELASHPTKSQTETIVNAVKDWCGIEQFDDDVSLLAFEWKGN

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=124, Percent_Identity=38.7096774193548, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787487, Length=209, Percent_Identity=26.7942583732057, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87082012, Length=119, Percent_Identity=31.0924369747899, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 44768; Mature: 44637

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKEILLVEDNKVMQLLMTKMLSSRGYQVVTAFDGVDAIAQLEKHPSIQLVLSDWMMPKL
CCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCEEEEECCCCCCCC
NGIELCYHLKSSDYGRYIFFVLLSGRDDEESIVEGINAGADDFVAKETNINELDARLKAG
CCEEEEEEECCCCCCEEEEEEEECCCCCHHHHHHHCCCCCCHHEECCCCHHHHHHHHHHC
FRSLELHNQLMSKNIELDRAYATIRQDLDSANALIRRMLPSQTEFLGVELHYTHIPSAQI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCHHH
GGDMLGCMQLDEEHVAIYLFDVAGHGVASALMSFAIQQSISSNSGTHSHVKGKKDTDPFY
CCCHHHHHEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
MLRDPSEVLARLNQSYLSQDNNNLYFTMVYAVLNIKSGLLSYASAGHPPMIWLQTQSQTA
EEECHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHH
HFIEQDSFVAGMFDFASYQTELVQLEQGDRILLYSDGITEAESASKEQYSEQGLKSLVQE
HHHHCCCHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
LASHPTKSQTETIVNAVKDWCGIEQFDDDVSLLAFEWKGN
HHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCC
>Mature Secondary Structure 
GKEILLVEDNKVMQLLMTKMLSSRGYQVVTAFDGVDAIAQLEKHPSIQLVLSDWMMPKL
CCEEEEECCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCEEEEECCCCCCCC
NGIELCYHLKSSDYGRYIFFVLLSGRDDEESIVEGINAGADDFVAKETNINELDARLKAG
CCEEEEEEECCCCCCEEEEEEEECCCCCHHHHHHHCCCCCCHHEECCCCHHHHHHHHHHC
FRSLELHNQLMSKNIELDRAYATIRQDLDSANALIRRMLPSQTEFLGVELHYTHIPSAQI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCHHH
GGDMLGCMQLDEEHVAIYLFDVAGHGVASALMSFAIQQSISSNSGTHSHVKGKKDTDPFY
CCCHHHHHEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
MLRDPSEVLARLNQSYLSQDNNNLYFTMVYAVLNIKSGLLSYASAGHPPMIWLQTQSQTA
EEECHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHH
HFIEQDSFVAGMFDFASYQTELVQLEQGDRILLYSDGITEAESASKEQYSEQGLKSLVQE
HHHHCCCHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
LASHPTKSQTETIVNAVKDWCGIEQFDDDVSLLAFEWKGN
HHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]