Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 157960076

GI number: 157960076

Start: 276830

End: 277699

Strand: Reverse

Name: gcvA [H]

Synonym: Spea_0247

Alternate gene names: 157960076

Gene position: 277699-276830 (Counterclockwise)

Preceding gene: 157960078

Following gene: 157960072

Centisome position: 5.37

GC content: 46.67

Gene sequence:

>870_bases
ATGCGCAAACTGCCTCCGCTTAGGGCGTTGCAAGTATTCGAAGCCGCTGCCAGACAGTCACATTTCTCACGCGCAGCCAC
AGAGCTTTGCATTACCCAAAGTGCCGTATCTCATCAAGTAAAACAGCTTGAAGAGCACTATGGCGAGCGGCTATTTAAGC
GTGAGGGACGCCAGCTACGACTGACTAGCAAAGGCGAGTTGCTATATAAAGAGTTAGAACGGATCTTTAATGAACTGAGT
GACTTAAGCATGCAAATAAGTGGCGACAGTAATGATCAGCTTCGCCTTGCCGTTTACAGCTCATTTGCGATCAAATGGCT
CATTCCAAGACTCAGCGACTTTAGACGCCAACATCCACAGATAAAGATCCGCCTCGATATGATTGCTGATGATCCCGAGT
TAAGCGACAGCGTGGCAGATATGTTTATCACCGGCCAAAGCGGCCAATTAGGTTACCATCATCACTTGTTGCATAAAGAG
CGCTTAATTCCGGTTATTAGCCCAAGCTTGATGGATTCAGATAGCTTAGCACTGGACGAATTAGCTAAACACCCATTGCT
AACTGTTGACGAAGGTCCACTCGGATTAGATTGGGACAGATGGTTTGTGGCAAATAATTTAGCTCTGCCTGCTGAGCAAC
AACAGCATATTTTTAGTCATGTGTTAATGGCGATTGAGGCAGCTATTGCTGGTCAAGGAATTGCCCTCGCATCGGACTTT
ATGGTGCAAGCCGACATCGAAAGGGGCTTGTTAGTTGAGTTGGATTTACCCGATATCCTGACTGGATTTGAATTCTACTT
CAGTTGTAAACAGCGTCGACTCAAAGAGCCTGCCATTGCCGCCTTTGTGACTTGGTTAAGCCTACAGTAG

Upstream 100 bases:

>100_bases
CCCGCAAAAGCTAAACTAAAATGATGTTTTTTAGCCATACATGAATTAAATTAATACTATGATAAAGATCACCTATTAAA
TTCATACTAGGACTCTAGCC

Downstream 100 bases:

>100_bases
CTAATCTATGAATAACCCAACAAAAAAGCCAGCGAATATCGCTGGCTTTCATTAACTCGCATCGAGTTAAACGATAAGAC
CGATCTTCTCGTAGACGTTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MRKLPPLRALQVFEAAARQSHFSRAATELCITQSAVSHQVKQLEEHYGERLFKREGRQLRLTSKGELLYKELERIFNELS
DLSMQISGDSNDQLRLAVYSSFAIKWLIPRLSDFRRQHPQIKIRLDMIADDPELSDSVADMFITGQSGQLGYHHHLLHKE
RLIPVISPSLMDSDSLALDELAKHPLLTVDEGPLGLDWDRWFVANNLALPAEQQQHIFSHVLMAIEAAIAGQGIALASDF
MVQADIERGLLVELDLPDILTGFEFYFSCKQRRLKEPAIAAFVTWLSLQ

Sequences:

>Translated_289_residues
MRKLPPLRALQVFEAAARQSHFSRAATELCITQSAVSHQVKQLEEHYGERLFKREGRQLRLTSKGELLYKELERIFNELS
DLSMQISGDSNDQLRLAVYSSFAIKWLIPRLSDFRRQHPQIKIRLDMIADDPELSDSVADMFITGQSGQLGYHHHLLHKE
RLIPVISPSLMDSDSLALDELAKHPLLTVDEGPLGLDWDRWFVANNLALPAEQQQHIFSHVLMAIEAAIAGQGIALASDF
MVQADIERGLLVELDLPDILTGFEFYFSCKQRRLKEPAIAAFVTWLSLQ
>Mature_289_residues
MRKLPPLRALQVFEAAARQSHFSRAATELCITQSAVSHQVKQLEEHYGERLFKREGRQLRLTSKGELLYKELERIFNELS
DLSMQISGDSNDQLRLAVYSSFAIKWLIPRLSDFRRQHPQIKIRLDMIADDPELSDSVADMFITGQSGQLGYHHHLLHKE
RLIPVISPSLMDSDSLALDELAKHPLLTVDEGPLGLDWDRWFVANNLALPAEQQQHIFSHVLMAIEAAIAGQGIALASDF
MVQADIERGLLVELDLPDILTGFEFYFSCKQRRLKEPAIAAFVTWLSLQ

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=290, Percent_Identity=38.2758620689655, Blast_Score=177, Evalue=6e-46,
Organism=Escherichia coli, GI1786448, Length=288, Percent_Identity=33.3333333333333, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788706, Length=296, Percent_Identity=31.0810810810811, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1790208, Length=295, Percent_Identity=28.135593220339, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1786401, Length=290, Percent_Identity=28.6206896551724, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790262, Length=284, Percent_Identity=25.7042253521127, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI157672245, Length=184, Percent_Identity=30.9782608695652, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI145693193, Length=302, Percent_Identity=25.4966887417219, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787589, Length=299, Percent_Identity=28.0936454849498, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787128, Length=142, Percent_Identity=33.8028169014084, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789283, Length=130, Percent_Identity=36.9230769230769, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87082132, Length=269, Percent_Identity=25.2788104089219, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787879, Length=194, Percent_Identity=27.319587628866, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788748, Length=126, Percent_Identity=31.7460317460317, Blast_Score=61, Evalue=8e-11,
Organism=Escherichia coli, GI1788296, Length=203, Percent_Identity=30.0492610837438, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32868; Mature: 32868

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLPPLRALQVFEAAARQSHFSRAATELCITQSAVSHQVKQLEEHYGERLFKREGRQLR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LTSKGELLYKELERIFNELSDLSMQISGDSNDQLRLAVYSSFAIKWLIPRLSDFRRQHPQ
ECCCCHHHHHHHHHHHHHHHHCEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCC
IKIRLDMIADDPELSDSVADMFITGQSGQLGYHHHLLHKERLIPVISPSLMDSDSLALDE
EEEEEEEECCCCCCCHHHHHEEEECCCCCCCHHHHHHHHHHCCHHCCCCCCCCCCHHHHH
LAKHPLLTVDEGPLGLDWDRWFVANNLALPAEQQQHIFSHVLMAIEAAIAGQGIALASDF
HHCCCEEEECCCCCCCCCHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHH
MVQADIERGLLVELDLPDILTGFEFYFSCKQRRLKEPAIAAFVTWLSLQ
HEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRKLPPLRALQVFEAAARQSHFSRAATELCITQSAVSHQVKQLEEHYGERLFKREGRQLR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LTSKGELLYKELERIFNELSDLSMQISGDSNDQLRLAVYSSFAIKWLIPRLSDFRRQHPQ
ECCCCHHHHHHHHHHHHHHHHCEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCC
IKIRLDMIADDPELSDSVADMFITGQSGQLGYHHHLLHKERLIPVISPSLMDSDSLALDE
EEEEEEEECCCCCCCHHHHHEEEECCCCCCCHHHHHHHHHHCCHHCCCCCCCCCCHHHHH
LAKHPLLTVDEGPLGLDWDRWFVANNLALPAEQQQHIFSHVLMAIEAAIAGQGIALASDF
HHCCCEEEECCCCCCCCCHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHH
MVQADIERGLLVELDLPDILTGFEFYFSCKQRRLKEPAIAAFVTWLSLQ
HEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]