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The map label for this gene is dam [H]

Identifier: 157960070

GI number: 157960070

Start: 272749

End: 273588

Strand: Direct

Name: dam [H]

Synonym: Spea_0241

Alternate gene names: 157960070

Gene position: 272749-273588 (Clockwise)

Preceding gene: 157960069

Following gene: 157960073

Centisome position: 5.27

GC content: 42.98

Gene sequence:

>840_bases
ATGATTAAAAAACAAAGAGCCTTTCTAAAGTGGGCAGGCGGAAAATTTAAACTGATTGAAGCCTTGGCAGAGCATTTGCC
TGAAGGTGATCGCCTTGTTGAGCCTTTTGTCGGTGCCGGTTCGGTATTTCTTAACAGCAATTACAGCCAATACCTTTTGT
GCGATATTAATCATGATTTGATTAATTTGTACAAGATCGTGAAAAAAGAGCCCGATAGATATATCGAAGCTGCGAAGGCG
CTTTTCGTTCCAGAGATGAATGATAAAGAGGCTTACTACAAAATCCGTACAGCCTTTAATGCGACTCAAGATCCTTTTAC
GCGCTCAGTCTATTTTCTTTATATGAATCGTCATGGTTTTAATGGCCTATGTCGTTATAACCGCAAAGGTGGTTTCAATG
TGCCATTTGGTTCATATAAAAAACCATACTTCCCAGAAAAAGAGATCCTTGCGTTTTCGCTTAAGGCGCAAAATGCCGAG
TTTCATTGCATCGGTTATGAGCAGGCGATCGATATGACGAAATCTGGCGATGTGGTTTATTGCGATCCGCCTTATGCTCC
TTTATCTTCGACTGCCAGCTTTACAACCTATGTAGGCAGCGGCTTCTCGTTAGACGATCAGGCATTGCTTGCTAGAAAAG
CACGTCATACGGCGATAGAAAGAGGCATTCCGGTACTGATTAGTAACCATGATATTCCGTTAACGCGTGAGCTATACCAT
GGCGCTCGTTTTGGCACGATACAGGTTCAACGTAACATCAGTCAGAAGGGGAGCAGTCGCAAGAAGGTCGATGAATTGAT
GGCCTTGTATGATGACTCTTACCATAGTGAAAACGACTAG

Upstream 100 bases:

>100_bases
TGAATATGAGTTGCAAGATGTACTTCCAACTCGTGAAATTCAATAATAAACAGAGTACAATCATCAGCTCTCTTTTTTTC
ACAGTGTTCAATCAATTTAA

Downstream 100 bases:

>100_bases
TCTAAATTTGCTGATTATTAAGTAAGTGCCGACTTATCCCAATCGGTATTAAGCGAGCACTTGCTTAACAATAAGCAGTA
AGCCAGCCACAAACACAATC

Product: DNA adenine methylase

Products: NA

Alternate protein names: DNA adenine methyltransferase; Deoxyadenosyl-methyltransferase; M.VchADam [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MIKKQRAFLKWAGGKFKLIEALAEHLPEGDRLVEPFVGAGSVFLNSNYSQYLLCDINHDLINLYKIVKKEPDRYIEAAKA
LFVPEMNDKEAYYKIRTAFNATQDPFTRSVYFLYMNRHGFNGLCRYNRKGGFNVPFGSYKKPYFPEKEILAFSLKAQNAE
FHCIGYEQAIDMTKSGDVVYCDPPYAPLSSTASFTTYVGSGFSLDDQALLARKARHTAIERGIPVLISNHDIPLTRELYH
GARFGTIQVQRNISQKGSSRKKVDELMALYDDSYHSEND

Sequences:

>Translated_279_residues
MIKKQRAFLKWAGGKFKLIEALAEHLPEGDRLVEPFVGAGSVFLNSNYSQYLLCDINHDLINLYKIVKKEPDRYIEAAKA
LFVPEMNDKEAYYKIRTAFNATQDPFTRSVYFLYMNRHGFNGLCRYNRKGGFNVPFGSYKKPYFPEKEILAFSLKAQNAE
FHCIGYEQAIDMTKSGDVVYCDPPYAPLSSTASFTTYVGSGFSLDDQALLARKARHTAIERGIPVLISNHDIPLTRELYH
GARFGTIQVQRNISQKGSSRKKVDELMALYDDSYHSEND
>Mature_279_residues
MIKKQRAFLKWAGGKFKLIEALAEHLPEGDRLVEPFVGAGSVFLNSNYSQYLLCDINHDLINLYKIVKKEPDRYIEAAKA
LFVPEMNDKEAYYKIRTAFNATQDPFTRSVYFLYMNRHGFNGLCRYNRKGGFNVPFGSYKKPYFPEKEILAFSLKAQNAE
FHCIGYEQAIDMTKSGDVVYCDPPYAPLSSTASFTTYVGSGFSLDDQALLARKARHTAIERGIPVLISNHDIPLTRELYH
GARFGTIQVQRNISQKGSSRKKVDELMALYDDSYHSEND

Specific function: Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=269, Percent_Identity=59.8513011152416, Blast_Score=339, Evalue=1e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 31820; Mature: 31820

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKQRAFLKWAGGKFKLIEALAEHLPEGDRLVEPFVGAGSVFLNSNYSQYLLCDINHDL
CCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCHHHHCCCCEEEEECCCCEEEEEECCHHH
INLYKIVKKEPDRYIEAAKALFVPEMNDKEAYYKIRTAFNATQDPFTRSVYFLYMNRHGF
HHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHEEEEEEHHCCCCCCCCCEEEEEEEECCCC
NGLCRYNRKGGFNVPFGSYKKPYFPEKEILAFSLKAQNAEFHCIGYEQAIDMTKSGDVVY
CCEEEECCCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCEEEEECHHHHHCCCCCCCEEE
CDPPYAPLSSTASFTTYVGSGFSLDDQALLARKARHTAIERGIPVLISNHDIPLTRELYH
ECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHC
GARFGTIQVQRNISQKGSSRKKVDELMALYDDSYHSEND
CCCCEEEEEECCHHHCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIKKQRAFLKWAGGKFKLIEALAEHLPEGDRLVEPFVGAGSVFLNSNYSQYLLCDINHDL
CCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCHHHHCCCCEEEEECCCCEEEEEECCHHH
INLYKIVKKEPDRYIEAAKALFVPEMNDKEAYYKIRTAFNATQDPFTRSVYFLYMNRHGF
HHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHEEEEEEHHCCCCCCCCCEEEEEEEECCCC
NGLCRYNRKGGFNVPFGSYKKPYFPEKEILAFSLKAQNAEFHCIGYEQAIDMTKSGDVVY
CCEEEECCCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCEEEEECHHHHHCCCCCCCEEE
CDPPYAPLSSTASFTTYVGSGFSLDDQALLARKARHTAIERGIPVLISNHDIPLTRELYH
ECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHC
GARFGTIQVQRNISQKGSSRKKVDELMALYDDSYHSEND
CCCCEEEEEECCHHHCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11705940 [H]