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The map label for this gene is murI

Identifier: 157959995

GI number: 157959995

Start: 180312

End: 181118

Strand: Direct

Name: murI

Synonym: Spea_0165

Alternate gene names: 157959995

Gene position: 180312-181118 (Clockwise)

Preceding gene: 157959993

Following gene: 157959997

Centisome position: 3.48

GC content: 42.26

Gene sequence:

>807_bases
TTGTTTGGACCTATTCTTATTTTTGACTCAGGGATCGGTGGACTATCGATTTTCGATGAGATCAGAAAAGTATTACCCGA
TCAAGATTGCTGCTATCTATTCGATAATGCCCGTTTACCTTATGGTGAGTTAGAAGAGTCGGTTCTTATTAAAGGTTGTG
TAGAGCTTATTTGCGAACAGGCACTTAAGATCGATGCATCATTAGTTGTTGTTGCCTGCAATTCGGCAAGTACTTTAGTG
CTCCCTGGTTTACGAGAAAGATTATCTATTCCTATTGTAGGGGTTGTGCCAGCAATTAAACCCGCTGCAAAGTTATCTAT
AGCAAGGCATATTGGTTTACTGGCAACGCCAGGTACGATTAAGCGATCCTATACCAGAGAGTTAATTGAACAATTTGCCG
ATGGATGCAAGGTAGAGCTTTTTGGCTCATCTGAACTCGTGATGTTGGCTGAGGCCAAACTTGCAGGGGAAAGCGTAGAT
TTGATTAAGCTGAGAGCTCAACTAGAGCCTATTCGTCAATCGAAGTTAGACACCCTAGTTCTAGGATGTACTCACTTTCC
TATAATAGCGAGCGAGATGCAGCAGATCTTAGGTAATGGAGTTAAGTTACTCGATTCAGGAGTTGCAATCGCCCAGAGGG
TCGTTTTTCTGCTTAACGAGTTTTCGCTGGATAAAGTCGTTGATAATAGAACGAAGAATTTAACGGCAATTTATACTACG
GCAGAGATATCGGCGGGTTTAACAACCAGACTAGCTGAAAAAGGCTTCACTAAGATAGTGTCACGCTCTTCAGCCAATCT
AGGTTAG

Upstream 100 bases:

>100_bases
AAATTCTAAAGCAATATAGCCAATCATGCTGTTTGTAAGACCTAACGACGTTTCTTTTATGTGTATCGAAATCATTATTA
CCCTATAGCGGAGAATACTT

Downstream 100 bases:

>100_bases
CTATACATCTTTATTATGCGTTATCACTTTTATCGGAATCTTGAGATTTAGCCTCACGCACTTTTAATGTTCTTTCTTGG
AAATCGAATTCATTCAATTT

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MFGPILIFDSGIGGLSIFDEIRKVLPDQDCCYLFDNARLPYGELEESVLIKGCVELICEQALKIDASLVVVACNSASTLV
LPGLRERLSIPIVGVVPAIKPAAKLSIARHIGLLATPGTIKRSYTRELIEQFADGCKVELFGSSELVMLAEAKLAGESVD
LIKLRAQLEPIRQSKLDTLVLGCTHFPIIASEMQQILGNGVKLLDSGVAIAQRVVFLLNEFSLDKVVDNRTKNLTAIYTT
AEISAGLTTRLAEKGFTKIVSRSSANLG

Sequences:

>Translated_268_residues
MFGPILIFDSGIGGLSIFDEIRKVLPDQDCCYLFDNARLPYGELEESVLIKGCVELICEQALKIDASLVVVACNSASTLV
LPGLRERLSIPIVGVVPAIKPAAKLSIARHIGLLATPGTIKRSYTRELIEQFADGCKVELFGSSELVMLAEAKLAGESVD
LIKLRAQLEPIRQSKLDTLVLGCTHFPIIASEMQQILGNGVKLLDSGVAIAQRVVFLLNEFSLDKVVDNRTKNLTAIYTT
AEISAGLTTRLAEKGFTKIVSRSSANLG
>Mature_268_residues
MFGPILIFDSGIGGLSIFDEIRKVLPDQDCCYLFDNARLPYGELEESVLIKGCVELICEQALKIDASLVVVACNSASTLV
LPGLRERLSIPIVGVVPAIKPAAKLSIARHIGLLATPGTIKRSYTRELIEQFADGCKVELFGSSELVMLAEAKLAGESVD
LIKLRAQLEPIRQSKLDTLVLGCTHFPIIASEMQQILGNGVKLLDSGVAIAQRVVFLLNEFSLDKVVDNRTKNLTAIYTT
AEISAGLTTRLAEKGFTKIVSRSSANLG

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family

Homologues:

Organism=Escherichia coli, GI87082355, Length=222, Percent_Identity=51.8018018018018, Blast_Score=224, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURI_SHEPA (A8GYV7)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001500029.1
- ProteinModelPortal:   A8GYV7
- SMR:   A8GYV7
- STRING:   A8GYV7
- GeneID:   5660566
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_0165
- NMPDR:   fig|398579.3.peg.147
- eggNOG:   COG0796
- HOGENOM:   HBG645102
- OMA:   CGHIPYG
- ProtClustDB:   PRK00865
- BioCyc:   SPEA398579:SPEA_0165-MONOMER
- HAMAP:   MF_00258
- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391
- Gene3D:   G3DSA:3.40.50.1860
- TIGRFAMs:   TIGR00067

Pfam domain/function: PF01177 Asp_Glu_race; SSF53681 Asp/Glu_race

EC number: =5.1.1.3

Molecular weight: Translated: 28915; Mature: 28915

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00923 ASP_GLU_RACEMASE_1; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGPILIFDSGIGGLSIFDEIRKVLPDQDCCYLFDNARLPYGELEESVLIKGCVELICEQ
CCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
ALKIDASLVVVACNSASTLVLPGLRERLSIPIVGVVPAIKPAAKLSIARHIGLLATPGTI
HHHCCCEEEEEEECCCCEEECCCHHHHHCCCEEEECCCCCHHHHHHHHHHHCEEECCCHH
KRSYTRELIEQFADGCKVELFGSSELVMLAEAKLAGESVDLIKLRAQLEPIRQSKLDTLV
HHHHHHHHHHHHHCCCEEEEECCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHEEE
LGCTHFPIIASEMQQILGNGVKLLDSGVAIAQRVVFLLNEFSLDKVVDNRTKNLTAIYTT
ECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEE
AEISAGLTTRLAEKGFTKIVSRSSANLG
HHHHCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFGPILIFDSGIGGLSIFDEIRKVLPDQDCCYLFDNARLPYGELEESVLIKGCVELICEQ
CCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
ALKIDASLVVVACNSASTLVLPGLRERLSIPIVGVVPAIKPAAKLSIARHIGLLATPGTI
HHHCCCEEEEEEECCCCEEECCCHHHHHCCCEEEECCCCCHHHHHHHHHHHCEEECCCHH
KRSYTRELIEQFADGCKVELFGSSELVMLAEAKLAGESVDLIKLRAQLEPIRQSKLDTLV
HHHHHHHHHHHHHCCCEEEEECCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHEEE
LGCTHFPIIASEMQQILGNGVKLLDSGVAIAQRVVFLLNEFSLDKVVDNRTKNLTAIYTT
ECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEE
AEISAGLTTRLAEKGFTKIVSRSSANLG
HHHHCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA