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The map label for this gene is selU [H]

Identifier: 157959990

GI number: 157959990

Start: 174763

End: 175860

Strand: Reverse

Name: selU [H]

Synonym: Spea_0160

Alternate gene names: 157959990

Gene position: 175860-174763 (Counterclockwise)

Preceding gene: 157959991

Following gene: 157959985

Centisome position: 3.4

GC content: 45.63

Gene sequence:

>1098_bases
ATGACACTCAATAAAGTGCCCGCAAGCGAATATCGCCGAATACTCGTTAACGATCATCCCATAATGGATGCGCGCGCACC
GGTAGAGTTTGAGAAAGGTGCCTTTCCTGCCAGCGTTAACCACCCCCTAATGGAGGATGAGGAGCGTAAAAAGGTGGGTA
CTTGCTATAAAGAACATGGCCAAGAGACCGCGATCAAGCTAGGCCATTCACTGGTTCAAGGTGAAATCAAGCAACAAAGA
GTCGATGCTTGGCTAGATTTTTTCACTAAGAATCCAGATGGATATCTTTATTGTTTTCGTGGTGGCCTACGTTCACAACT
GACCCAGCAATGGCTAAAAGAAGCAGGCCTAGATATCCCCTTTATTGAAGGCGGCTATAAGGCCATGCGCCAGTTTCTGA
TTGAAACCATAGATAATGCAGCCAATGTAAAGCCGATGCTTATCCTAAGCGGAATAACTGGCAGTGGTAAAACTGACTTC
CTTTTGCAGCGTAAAGAAGCCGTTGATCTTGAAGGAATAGCGCACCACAGAGGTTCCAGCTTTGGTCGCTATCACGAAGG
GCAACCGAGCCAAATCAACTTTGAAAATGCCCTCGCTGTTGCACTGTTAAAGCATCAAGACACTGATGCAAAGCACTTGC
TACTCGAAGATGAAAGCTTCCTGATTGGTCGTTCTGCATTACCCCAGGCTTTCTATTCAGGCATGCAGACAGCCAGCGTA
TTGGTGTTAGAAGAGTCGATGGATGCAAGACTGACGCGCCTACTTAACGAATATGTGCACAAGATGCATAGTGGTTATAT
CCAAAGGCTAGGTGAAGAAGCAGGCTTCGAAGCTTTTGCCCAATACTTGGCCCAAAGCATCACAGGCATTAAAAAGCGCC
TAGGTGGAAAACAACATGACGAGCTTCAGGCAATGATCACTGATGCCTTAAGTATTCAAACGAGTCAAAATGACACCCGT
GGTCATTTAGGCTGGATAGAGCTGTTGCTGGTCAAGTATTACGACCCTATGTATCAATATCAAATCGATAAGAAAGCCGA
ACGTATTTTATTTAAAGGCGACCATCAAGCTATGCATCAGTGGCTAGATGACCATTAA

Upstream 100 bases:

>100_bases
AGGCCGCTCTACAAACGCTGCTTGCTGACAATGGCATCGAACCTATCTGTATCGGCCAGTTAGTTGAAAAAAATGCGGCA
ATGACCGTGGAGCTCATCTA

Downstream 100 bases:

>100_bases
GCACCGACTTTGCTCTAAGCCCATCCCATTAGCTCGCTGTTACCGGCAGCTAATGGGGTTATTTCTAACTTCAGTACTCT
TCCCTCAATAGCAGTAAATT

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTLNKVPASEYRRILVNDHPIMDARAPVEFEKGAFPASVNHPLMEDEERKKVGTCYKEHGQETAIKLGHSLVQGEIKQQR
VDAWLDFFTKNPDGYLYCFRGGLRSQLTQQWLKEAGLDIPFIEGGYKAMRQFLIETIDNAANVKPMLILSGITGSGKTDF
LLQRKEAVDLEGIAHHRGSSFGRYHEGQPSQINFENALAVALLKHQDTDAKHLLLEDESFLIGRSALPQAFYSGMQTASV
LVLEESMDARLTRLLNEYVHKMHSGYIQRLGEEAGFEAFAQYLAQSITGIKKRLGGKQHDELQAMITDALSIQTSQNDTR
GHLGWIELLLVKYYDPMYQYQIDKKAERILFKGDHQAMHQWLDDH

Sequences:

>Translated_365_residues
MTLNKVPASEYRRILVNDHPIMDARAPVEFEKGAFPASVNHPLMEDEERKKVGTCYKEHGQETAIKLGHSLVQGEIKQQR
VDAWLDFFTKNPDGYLYCFRGGLRSQLTQQWLKEAGLDIPFIEGGYKAMRQFLIETIDNAANVKPMLILSGITGSGKTDF
LLQRKEAVDLEGIAHHRGSSFGRYHEGQPSQINFENALAVALLKHQDTDAKHLLLEDESFLIGRSALPQAFYSGMQTASV
LVLEESMDARLTRLLNEYVHKMHSGYIQRLGEEAGFEAFAQYLAQSITGIKKRLGGKQHDELQAMITDALSIQTSQNDTR
GHLGWIELLLVKYYDPMYQYQIDKKAERILFKGDHQAMHQWLDDH
>Mature_364_residues
TLNKVPASEYRRILVNDHPIMDARAPVEFEKGAFPASVNHPLMEDEERKKVGTCYKEHGQETAIKLGHSLVQGEIKQQRV
DAWLDFFTKNPDGYLYCFRGGLRSQLTQQWLKEAGLDIPFIEGGYKAMRQFLIETIDNAANVKPMLILSGITGSGKTDFL
LQRKEAVDLEGIAHHRGSSFGRYHEGQPSQINFENALAVALLKHQDTDAKHLLLEDESFLIGRSALPQAFYSGMQTASVL
VLEESMDARLTRLLNEYVHKMHSGYIQRLGEEAGFEAFAQYLAQSITGIKKRLGGKQHDELQAMITDALSIQTSQNDTRG
HLGWIELLLVKYYDPMYQYQIDKKAERILFKGDHQAMHQWLDDH

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=357, Percent_Identity=47.0588235294118, Blast_Score=345, Evalue=2e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: NA

Molecular weight: Translated: 41493; Mature: 41362

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLNKVPASEYRRILVNDHPIMDARAPVEFEKGAFPASVNHPLMEDEERKKVGTCYKEHG
CCCCCCCHHHHHEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
QETAIKLGHSLVQGEIKQQRVDAWLDFFTKNPDGYLYCFRGGLRSQLTQQWLKEAGLDIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCC
FIEGGYKAMRQFLIETIDNAANVKPMLILSGITGSGKTDFLLQRKEAVDLEGIAHHRGSS
EECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHCCCC
FGRYHEGQPSQINFENALAVALLKHQDTDAKHLLLEDESFLIGRSALPQAFYSGMQTASV
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCHHHCCHHHHHHHHCCCCHHEE
LVLEESMDARLTRLLNEYVHKMHSGYIQRLGEEAGFEAFAQYLAQSITGIKKRLGGKQHD
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHH
ELQAMITDALSIQTSQNDTRGHLGWIELLLVKYYDPMYQYQIDKKAERILFKGDHQAMHQ
HHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHEECCCHHHHHH
WLDDH
HHCCC
>Mature Secondary Structure 
TLNKVPASEYRRILVNDHPIMDARAPVEFEKGAFPASVNHPLMEDEERKKVGTCYKEHG
CCCCCCHHHHHEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
QETAIKLGHSLVQGEIKQQRVDAWLDFFTKNPDGYLYCFRGGLRSQLTQQWLKEAGLDIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCC
FIEGGYKAMRQFLIETIDNAANVKPMLILSGITGSGKTDFLLQRKEAVDLEGIAHHRGSS
EECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHCCCC
FGRYHEGQPSQINFENALAVALLKHQDTDAKHLLLEDESFLIGRSALPQAFYSGMQTASV
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCHHHCCHHHHHHHHCCCCHHEE
LVLEESMDARLTRLLNEYVHKMHSGYIQRLGEEAGFEAFAQYLAQSITGIKKRLGGKQHD
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHH
ELQAMITDALSIQTSQNDTRGHLGWIELLLVKYYDPMYQYQIDKKAERILFKGDHQAMHQ
HHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHEECCCHHHHHH
WLDDH
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA