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The map label for this gene is pckA [H]

Identifier: 157959970

GI number: 157959970

Start: 155440

End: 156981

Strand: Reverse

Name: pckA [H]

Synonym: Spea_0140

Alternate gene names: 157959970

Gene position: 156981-155440 (Counterclockwise)

Preceding gene: 157959971

Following gene: 157959969

Centisome position: 3.03

GC content: 48.96

Gene sequence:

>1542_bases
ATGGCGGATGGATCAAACCGCGAGTATCTAAACCTTTCAACAGGCCAGTTAGTCGAGCTTGCGTTAGCGCGTGGAGAAGG
CCAATTGACCGCGAATGGCGCTTTAGTTGCAAAGACTGGTGAACGCTCAGGCCGTTCGCCTAACGACCGTTTCATCGTTA
AAGAGCCAAGCTCTGAAGCAGATATCGAATGGGGTCCAGTAAACAAGCCTTTCAAAGCCGATGCCTTTACTGCACTTTGG
AACCGAGTCGAAGCTTTTCTAGCTGACAAAGACACTTTCGTATCAAATCTCGAAGTCGGTGCCAGCAAAGAGCATTACCA
GCCAGTAAAAGTCACTACTCAGTATGCTTGGCACCAACTATTTGCTCGTAACCTATTCATAGTGCCAGAAGAATTTAATG
CCGCCGATAAGCCTGTATGGCAGATCATCAACGCACCTGGTTTTGAGTGTGTTCCTGAGCGAGACGGTACTCATTCTGAT
GCGACAGTTATCATCAACTTCGCAGAGCGTAAGGTGCTACTTGCTGGCCTTAAGTACGCTGGTGAAATGAAGAAGTCGAT
GTTCTCGGTACAAAACTTCTTACTACCCGCTAAAGGTGTGCTACCAATGCACTGCTCGGCAAACGTCGGTGCCGATGGCG
ATACCACTCTGTTCTTCGGTCTATCGGGTACAGGTAAAACTACACTTTCAGCCGACCCTAAACGCTTCTTAATTGGTGAC
GATGAACACGGCTGGGCACCAGGCGGCGTATTCAACATCGAAGGTGGCTGCTATGCAAAGTGTATCGACCTAAGCCAGAA
GAACGAGCCTGTCATTTGGGATGCTATTCGCTTTGGTACCGTGCTTGAAAACGTAGTAACCGATGAAAATCGCGTACCCG
ACTACACAAATTCAAGCTTAACCGAAAACACCCGCGCCGCTTATCCACTCGAGCATATTGCCCAGCGTAAACTAGATAAC
TGTGGCGCTGAGCCTCACGCAGTTGTGTTCTTAACTTGTGACGTTTCTGGCGTGTTACCACCAGTATCTAAGTTAACTAA
AGAGCAAGCGGCTTACCACTTCTTGTCTGGTTATACCGCAAAAGTTGGCTCGACGGAGATGGGGTCTACTGCCGCGATAC
AGTCGACATTCTCGACCTGCTTCGGTGCGCCGTTCTTCCCTCGTCCTGCAGGAGTTTACGCTGAGCTACTGATGAAGCGT
ATCGAGTCTTTTGGCAGTCAAGTTTACCTAGTTAACACTGGCTGGACAGGCGGGCCTCACGGCATTGGTAAGCGTTTCGA
TATCCCAACAACTCGCGCCATTGTCGATGCCATCGTGTCTGGTGAGCTTAAAGATGTCGAGACAGAGTACCTAGAAAAGC
TAAACCTACATGTACCGGTTGCCATTCCAGGTGTAGACAGCAAGCTGCTTAACCCAATCAACACTTGGGAAGACAAGGTC
CAGTATGCCGAATTTGCTCAGCACCTTGCAGATAGCTTTAAGGCTAACTTCGAAAAGTATCAGGTACCTGATTCAATCAA
GAACGCAGGCCCTAACGCTTAA

Upstream 100 bases:

>100_bases
GTACACATATTCATATCCGCATACGTTACAATCGCCGTAAATATAAGCTGGCGCAATAGATCAGCACCGAATAATGACCT
CACAGATGGAGACCTTACCT

Downstream 100 bases:

>100_bases
TTCGCTTTGTTCTAGCGCTCTTTAAAGCCATCGGTGAATACTGTGGCCCAATTAAAATCCCAGCTATATGCTGGGATTTT
TTATGAATAAACCTAAGCCA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MADGSNREYLNLSTGQLVELALARGEGQLTANGALVAKTGERSGRSPNDRFIVKEPSSEADIEWGPVNKPFKADAFTALW
NRVEAFLADKDTFVSNLEVGASKEHYQPVKVTTQYAWHQLFARNLFIVPEEFNAADKPVWQIINAPGFECVPERDGTHSD
ATVIINFAERKVLLAGLKYAGEMKKSMFSVQNFLLPAKGVLPMHCSANVGADGDTTLFFGLSGTGKTTLSADPKRFLIGD
DEHGWAPGGVFNIEGGCYAKCIDLSQKNEPVIWDAIRFGTVLENVVTDENRVPDYTNSSLTENTRAAYPLEHIAQRKLDN
CGAEPHAVVFLTCDVSGVLPPVSKLTKEQAAYHFLSGYTAKVGSTEMGSTAAIQSTFSTCFGAPFFPRPAGVYAELLMKR
IESFGSQVYLVNTGWTGGPHGIGKRFDIPTTRAIVDAIVSGELKDVETEYLEKLNLHVPVAIPGVDSKLLNPINTWEDKV
QYAEFAQHLADSFKANFEKYQVPDSIKNAGPNA

Sequences:

>Translated_513_residues
MADGSNREYLNLSTGQLVELALARGEGQLTANGALVAKTGERSGRSPNDRFIVKEPSSEADIEWGPVNKPFKADAFTALW
NRVEAFLADKDTFVSNLEVGASKEHYQPVKVTTQYAWHQLFARNLFIVPEEFNAADKPVWQIINAPGFECVPERDGTHSD
ATVIINFAERKVLLAGLKYAGEMKKSMFSVQNFLLPAKGVLPMHCSANVGADGDTTLFFGLSGTGKTTLSADPKRFLIGD
DEHGWAPGGVFNIEGGCYAKCIDLSQKNEPVIWDAIRFGTVLENVVTDENRVPDYTNSSLTENTRAAYPLEHIAQRKLDN
CGAEPHAVVFLTCDVSGVLPPVSKLTKEQAAYHFLSGYTAKVGSTEMGSTAAIQSTFSTCFGAPFFPRPAGVYAELLMKR
IESFGSQVYLVNTGWTGGPHGIGKRFDIPTTRAIVDAIVSGELKDVETEYLEKLNLHVPVAIPGVDSKLLNPINTWEDKV
QYAEFAQHLADSFKANFEKYQVPDSIKNAGPNA
>Mature_512_residues
ADGSNREYLNLSTGQLVELALARGEGQLTANGALVAKTGERSGRSPNDRFIVKEPSSEADIEWGPVNKPFKADAFTALWN
RVEAFLADKDTFVSNLEVGASKEHYQPVKVTTQYAWHQLFARNLFIVPEEFNAADKPVWQIINAPGFECVPERDGTHSDA
TVIINFAERKVLLAGLKYAGEMKKSMFSVQNFLLPAKGVLPMHCSANVGADGDTTLFFGLSGTGKTTLSADPKRFLIGDD
EHGWAPGGVFNIEGGCYAKCIDLSQKNEPVIWDAIRFGTVLENVVTDENRVPDYTNSSLTENTRAAYPLEHIAQRKLDNC
GAEPHAVVFLTCDVSGVLPPVSKLTKEQAAYHFLSGYTAKVGSTEMGSTAAIQSTFSTCFGAPFFPRPAGVYAELLMKRI
ESFGSQVYLVNTGWTGGPHGIGKRFDIPTTRAIVDAIVSGELKDVETEYLEKLNLHVPVAIPGVDSKLLNPINTWEDKVQ
YAEFAQHLADSFKANFEKYQVPDSIKNAGPNA

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=489, Percent_Identity=47.0347648261759, Blast_Score=407, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6322950, Length=514, Percent_Identity=45.3307392996109, Blast_Score=413, Evalue=1e-116,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 55997; Mature: 55865

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADGSNREYLNLSTGQLVELALARGEGQLTANGALVAKTGERSGRSPNDRFIVKEPSSEA
CCCCCCCCEEECCCCHHHHHHHHCCCCEEEECCEEEEECCCCCCCCCCCCEEEECCCCCC
DIEWGPVNKPFKADAFTALWNRVEAFLADKDTFVSNLEVGASKEHYQPVKVTTQYAWHQL
CEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCEEEHHHHHHHHH
FARNLFIVPEEFNAADKPVWQIINAPGFECVPERDGTHSDATVIINFAERKVLLAGLKYA
HHCCEEEEECCCCCCCCCHHHHHCCCCCEECCCCCCCCCCCEEEEEECCCEEHHHHHHHH
GEMKKSMFSVQNFLLPAKGVLPMHCSANVGADGDTTLFFGLSGTGKTTLSADPKRFLIGD
HHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCEEECCCCCEEEECC
DEHGWAPGGVFNIEGGCYAKCIDLSQKNEPVIWDAIRFGTVLENVVTDENRVPDYTNSSL
CCCCCCCCCEEEECCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCC
TENTRAAYPLEHIAQRKLDNCGAEPHAVVFLTCDVSGVLPPVSKLTKEQAAYHFLSGYTA
CCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEE
KVGSTEMGSTAAIQSTFSTCFGAPFFPRPAGVYAELLMKRIESFGSQVYLVNTGWTGGPH
ECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCC
GIGKRFDIPTTRAIVDAIVSGELKDVETEYLEKLNLHVPVAIPGVDSKLLNPINTWEDKV
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCHHHCCCHHHHHHHH
QYAEFAQHLADSFKANFEKYQVPDSIKNAGPNA
HHHHHHHHHHHHHHCCCHHEECCHHHHCCCCCC
>Mature Secondary Structure 
ADGSNREYLNLSTGQLVELALARGEGQLTANGALVAKTGERSGRSPNDRFIVKEPSSEA
CCCCCCCEEECCCCHHHHHHHHCCCCEEEECCEEEEECCCCCCCCCCCCEEEECCCCCC
DIEWGPVNKPFKADAFTALWNRVEAFLADKDTFVSNLEVGASKEHYQPVKVTTQYAWHQL
CEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCEEEHHHHHHHHH
FARNLFIVPEEFNAADKPVWQIINAPGFECVPERDGTHSDATVIINFAERKVLLAGLKYA
HHCCEEEEECCCCCCCCCHHHHHCCCCCEECCCCCCCCCCCEEEEEECCCEEHHHHHHHH
GEMKKSMFSVQNFLLPAKGVLPMHCSANVGADGDTTLFFGLSGTGKTTLSADPKRFLIGD
HHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCEEECCCCCEEEECC
DEHGWAPGGVFNIEGGCYAKCIDLSQKNEPVIWDAIRFGTVLENVVTDENRVPDYTNSSL
CCCCCCCCCEEEECCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCC
TENTRAAYPLEHIAQRKLDNCGAEPHAVVFLTCDVSGVLPPVSKLTKEQAAYHFLSGYTA
CCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEE
KVGSTEMGSTAAIQSTFSTCFGAPFFPRPAGVYAELLMKRIESFGSQVYLVNTGWTGGPH
ECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCC
GIGKRFDIPTTRAIVDAIVSGELKDVETEYLEKLNLHVPVAIPGVDSKLLNPINTWEDKV
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCHHHCCCHHHHHHHH
QYAEFAQHLADSFKANFEKYQVPDSIKNAGPNA
HHHHHHHHHHHHHHCCCHHEECCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA