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The map label for this gene is gltS [H]

Identifier: 157959948

GI number: 157959948

Start: 131327

End: 132550

Strand: Direct

Name: gltS [H]

Synonym: Spea_0118

Alternate gene names: 157959948

Gene position: 131327-132550 (Clockwise)

Preceding gene: 157959947

Following gene: 157959949

Centisome position: 2.54

GC content: 45.1

Gene sequence:

>1224_bases
ATGAATACTGTTTATTCAATCGGAGAACTAGAATCATTTTTGGTCGCCATCTTGGTACTGTTTATCGGCCACTCAGTTAA
CCGCCACGTTCCTTTTTTCAAAAAGTATAATATCCCAGAACCTATTATCGGTGGCCTGGTTGTCGCAGCCGTTATCACTG
TTCTTCATTTTCAAAATATTACGCTAGCGTTTTCTCTGCCGATGCAGAACACGCTGATGCTGATGTTCTTCAGCACCGTG
GGCTTAGCTGCCAGTTATAAACTGCTAGCCCGTGGTGGTAAAAAAGTCTTTATTTTTCTTGGGATTGCCTCGATTTACAT
CATATTGCAGAATGCAGTAGGCGTGAGTTTGGCGGCGGCGCTAGGCTTGGAAGCATTGATGGGCTTGGTTGCTGGCTCCA
TTACTCTTTCGGGTGGCCACGGTACTGGTGCCGCCTGGTCGCAGACGTTTGCCGAGCAGTACAATATGAATACCCTAGAG
TTCGCTATGGCCGCAGCTACATTCGGTTTGGTGATGGGGGGAATTATTGGCGGCCCTGTGGCACAAAGACTCATCGATAA
GCATAAGCTTGAATCCTCTTACGGTATTGGTGGTAACCATCATACGGACCATCCCGATCTAGTAACCTACGATCAGTTAG
AAGTGGATAATGTTACTGCCAAGAGTGTTCTAGAAACCCTATTTATTATCATGTTATGTGTGGCTGGGGCTAAATGGGTC
GGGCATCTAGTTGCTAGTTTTGATATCAGTTGGTTGAAGATGCCGGACTTTGTCTATGCCCTATTTCTTGGGGTGGTGAT
CACTAACTTCACCGAGATGACTCGTGGCTATAAGATGAATAACGAATGTGTCGATATCTTAGGTACTGTCGCCTTATCTC
TATTTCTTGCTATGGCATTAATGAGCCTGAAGTTGTGGGAGATCTTTGACCTAGCGATTCCGTTATTGATTATTTTAATG
GTGCAAACCGTTGTTTTAGCCTGTTTTGCATACTTTGTTACCTTCAAAGTGATGGGTTCTAACTATGATGCAGCCGTGAT
GGCGGGGGGGCACTGTGGTTTTGGTATGGGGGCGACACCTACCGCGGTTATGAATATGGGAGCCCTAGTTTCACGTAATG
GTCCATCGCCACAAGCTTTTATGGTGGTGCCTATTGTCGGTGCTTTCTTTATCGACATTGTGAATCTAGTCGTATTGCAG
GGTTATATAAAATTTATCTTATAG

Upstream 100 bases:

>100_bases
GCTAAGACTAATATTATTTACCCTAATGAATATGACAGAATAGCCGGCTAATTTTGGTTTTGTAGGTTTTTCACTACAAA
ACCTAACGGATAGGAGTTTC

Downstream 100 bases:

>100_bases
TGGACAGTATCTATTTCAAAAAAGGCACGCATTAGCGTGCCTTTTTTATTGGACTAGAATTAATTTTGACCTTGATGCAA
TCAATAACGATTGAGATTCG

Product: sodium/glutamate symporter

Products: Na (I) [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate permease [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MNTVYSIGELESFLVAILVLFIGHSVNRHVPFFKKYNIPEPIIGGLVVAAVITVLHFQNITLAFSLPMQNTLMLMFFSTV
GLAASYKLLARGGKKVFIFLGIASIYIILQNAVGVSLAAALGLEALMGLVAGSITLSGGHGTGAAWSQTFAEQYNMNTLE
FAMAAATFGLVMGGIIGGPVAQRLIDKHKLESSYGIGGNHHTDHPDLVTYDQLEVDNVTAKSVLETLFIIMLCVAGAKWV
GHLVASFDISWLKMPDFVYALFLGVVITNFTEMTRGYKMNNECVDILGTVALSLFLAMALMSLKLWEIFDLAIPLLIILM
VQTVVLACFAYFVTFKVMGSNYDAAVMAGGHCGFGMGATPTAVMNMGALVSRNGPSPQAFMVVPIVGAFFIDIVNLVVLQ
GYIKFIL

Sequences:

>Translated_407_residues
MNTVYSIGELESFLVAILVLFIGHSVNRHVPFFKKYNIPEPIIGGLVVAAVITVLHFQNITLAFSLPMQNTLMLMFFSTV
GLAASYKLLARGGKKVFIFLGIASIYIILQNAVGVSLAAALGLEALMGLVAGSITLSGGHGTGAAWSQTFAEQYNMNTLE
FAMAAATFGLVMGGIIGGPVAQRLIDKHKLESSYGIGGNHHTDHPDLVTYDQLEVDNVTAKSVLETLFIIMLCVAGAKWV
GHLVASFDISWLKMPDFVYALFLGVVITNFTEMTRGYKMNNECVDILGTVALSLFLAMALMSLKLWEIFDLAIPLLIILM
VQTVVLACFAYFVTFKVMGSNYDAAVMAGGHCGFGMGATPTAVMNMGALVSRNGPSPQAFMVVPIVGAFFIDIVNLVVLQ
GYIKFIL
>Mature_407_residues
MNTVYSIGELESFLVAILVLFIGHSVNRHVPFFKKYNIPEPIIGGLVVAAVITVLHFQNITLAFSLPMQNTLMLMFFSTV
GLAASYKLLARGGKKVFIFLGIASIYIILQNAVGVSLAAALGLEALMGLVAGSITLSGGHGTGAAWSQTFAEQYNMNTLE
FAMAAATFGLVMGGIIGGPVAQRLIDKHKLESSYGIGGNHHTDHPDLVTYDQLEVDNVTAKSVLETLFIIMLCVAGAKWV
GHLVASFDISWLKMPDFVYALFLGVVITNFTEMTRGYKMNNECVDILGTVALSLFLAMALMSLKLWEIFDLAIPLLIILM
VQTVVLACFAYFVTFKVMGSNYDAAVMAGGHCGFGMGATPTAVMNMGALVSRNGPSPQAFMVVPIVGAFFIDIVNLVVLQ
GYIKFIL

Specific function: Catalyzes the sodium-dependent uptake of extracellular glutamate [H]

COG id: COG0786

COG function: function code E; Na+/glutamate symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gltS symporter family [H]

Homologues:

Organism=Escherichia coli, GI1790085, Length=384, Percent_Identity=48.6979166666667, Blast_Score=301, Evalue=7e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004445 [H]

Pfam domain/function: PF03616 Glt_symporter [H]

EC number: NA

Molecular weight: Translated: 43794; Mature: 43794

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTVYSIGELESFLVAILVLFIGHSVNRHVPFFKKYNIPEPIIGGLVVAAVITVLHFQNI
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCE
TLAFSLPMQNTLMLMFFSTVGLAASYKLLARGGKKVFIFLGIASIYIILQNAVGVSLAAA
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
LGLEALMGLVAGSITLSGGHGTGAAWSQTFAEQYNMNTLEFAMAAATFGLVMGGIIGGPV
HHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHH
AQRLIDKHKLESSYGIGGNHHTDHPDLVTYDQLEVDNVTAKSVLETLFIIMLCVAGAKWV
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCEECCCCHHHHHHHHHHHHHHHHHHHHHH
GHLVASFDISWLKMPDFVYALFLGVVITNFTEMTRGYKMNNECVDILGTVALSLFLAMAL
HHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
MSLKLWEIFDLAIPLLIILMVQTVVLACFAYFVTFKVMGSNYDAAVMAGGHCGFGMGATP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCH
TAVMNMGALVSRNGPSPQAFMVVPIVGAFFIDIVNLVVLQGYIKFIL
HHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MNTVYSIGELESFLVAILVLFIGHSVNRHVPFFKKYNIPEPIIGGLVVAAVITVLHFQNI
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCE
TLAFSLPMQNTLMLMFFSTVGLAASYKLLARGGKKVFIFLGIASIYIILQNAVGVSLAAA
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
LGLEALMGLVAGSITLSGGHGTGAAWSQTFAEQYNMNTLEFAMAAATFGLVMGGIIGGPV
HHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHH
AQRLIDKHKLESSYGIGGNHHTDHPDLVTYDQLEVDNVTAKSVLETLFIIMLCVAGAKWV
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCEECCCCHHHHHHHHHHHHHHHHHHHHHH
GHLVASFDISWLKMPDFVYALFLGVVITNFTEMTRGYKMNNECVDILGTVALSLFLAMAL
HHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
MSLKLWEIFDLAIPLLIILMVQTVVLACFAYFVTFKVMGSNYDAAVMAGGHCGFGMGATP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCH
TAVMNMGALVSRNGPSPQAFMVVPIVGAFFIDIVNLVVLQGYIKFIL
HHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-glutamate [Periplasm] = Na (I) [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]