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The map label for this gene is pldB [H]

Identifier: 157959924

GI number: 157959924

Start: 103963

End: 104937

Strand: Reverse

Name: pldB [H]

Synonym: Spea_0094

Alternate gene names: 157959924

Gene position: 104937-103963 (Counterclockwise)

Preceding gene: 157959925

Following gene: 157959916

Centisome position: 2.03

GC content: 49.54

Gene sequence:

>975_bases
ATGTCGATTGCTACCGAGCCCTATATGAACACCAATACTGCTGTTAGCTTTTCATCAGAACATCATCTCAACAGTCCTGA
ACAAAAGGTGTTTTGGCAGCAAGTGACGCAATCGAGCTTCAAGACTCGTGATGACATTAGCTTAGCCTACGCCCGTATCA
CTCATCCAAATAGTATTGGCACCATAGTGATCAGCAGTGGGCGAGTCGAATCCTATCTAAAATACAAAGAACTGATGTTT
GACCTGTATCAGCAAGGCTACAGCTTGTTTGCCATCGATCATCGTGGCCAAGGGTTATCGGCACGCACAACCGCTAATCC
TCATCAGGGACATATCGACAAGTTCCAGCGATATGTCGACGACTTCGCCGAATTTGTCACGAAAATAGTCAAACCGCATG
CAATGGAAAACTACTACCTAGTTGGCCATTCAATGGGGGGCGCAATCGGTACGCTCTATCTAAGTCAGTACCCGGCAACC
TTTAAGGCCGCGGTGTTTTCGGCGCCTATGTATGGCATCAAGCTACCCCTTCCCAAAGCCTTTATCCGCGGCCTAGCGTC
TTTACTCGATAGCAGCAAACAGCCTAACTATATATTGGGTGGCAAAGGCTACCATGCAGAGCCGTTCGAGAAGAACGACC
TGACCCACAGCAAGCTGCGCTATGACGATTATCGCGAGCTATACCGCCAGCAGCCACAGCTGCAGCTAGGTTCGCCAACC
AACCATTGGTTAGTCGAGTCCATCGATGCTGCCGAGCAGGCGGTAGATGCCGCTCGAAATAGTACTATCCCGCTGCTAAT
CTTGCAGGCAGAAGAAGATACTATCGTCGCTAATGCCGCGCAGAATCGCGCCGTAAACGGCCAGTGCCGCTTAATTAAAA
TCCCCTATGCCCGTCATGAGATCTTTATGGAGCGAGACAAGCCGCGCAACCTTGCGCTCAACGCATTATGCGAGTTCTTA
AAGCAAATTAACTAA

Upstream 100 bases:

>100_bases
GGCCTTTTTAATGGGCTCTTTTTAATCGCTTAGGTTTCGCTAGAGTCTGGCTAAACAAGTTTGCTACACTGCTTACAAGA
TCACTCAATAGAACCATTCC

Downstream 100 bases:

>100_bases
TAGGCTAACGAGCAATAAAAAAGGGATAGCGAGCTATCCCTTTTTCGTATGTGGCCGATTAACCTAACTGCCCCGCCACC
ATCAGCTCCCGCGTCTCGGG

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: Lecithinase B [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSIATEPYMNTNTAVSFSSEHHLNSPEQKVFWQQVTQSSFKTRDDISLAYARITHPNSIGTIVISSGRVESYLKYKELMF
DLYQQGYSLFAIDHRGQGLSARTTANPHQGHIDKFQRYVDDFAEFVTKIVKPHAMENYYLVGHSMGGAIGTLYLSQYPAT
FKAAVFSAPMYGIKLPLPKAFIRGLASLLDSSKQPNYILGGKGYHAEPFEKNDLTHSKLRYDDYRELYRQQPQLQLGSPT
NHWLVESIDAAEQAVDAARNSTIPLLILQAEEDTIVANAAQNRAVNGQCRLIKIPYARHEIFMERDKPRNLALNALCEFL
KQIN

Sequences:

>Translated_324_residues
MSIATEPYMNTNTAVSFSSEHHLNSPEQKVFWQQVTQSSFKTRDDISLAYARITHPNSIGTIVISSGRVESYLKYKELMF
DLYQQGYSLFAIDHRGQGLSARTTANPHQGHIDKFQRYVDDFAEFVTKIVKPHAMENYYLVGHSMGGAIGTLYLSQYPAT
FKAAVFSAPMYGIKLPLPKAFIRGLASLLDSSKQPNYILGGKGYHAEPFEKNDLTHSKLRYDDYRELYRQQPQLQLGSPT
NHWLVESIDAAEQAVDAARNSTIPLLILQAEEDTIVANAAQNRAVNGQCRLIKIPYARHEIFMERDKPRNLALNALCEFL
KQIN
>Mature_323_residues
SIATEPYMNTNTAVSFSSEHHLNSPEQKVFWQQVTQSSFKTRDDISLAYARITHPNSIGTIVISSGRVESYLKYKELMFD
LYQQGYSLFAIDHRGQGLSARTTANPHQGHIDKFQRYVDDFAEFVTKIVKPHAMENYYLVGHSMGGAIGTLYLSQYPATF
KAAVFSAPMYGIKLPLPKAFIRGLASLLDSSKQPNYILGGKGYHAEPFEKNDLTHSKLRYDDYRELYRQQPQLQLGSPTN
HWLVESIDAAEQAVDAARNSTIPLLILQAEEDTIVANAAQNRAVNGQCRLIKIPYARHEIFMERDKPRNLALNALCEFLK
QIN

Specific function: Unknown

COG id: COG2267

COG function: function code I; Lysophospholipase

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI48994980, Length=305, Percent_Identity=37.7049180327869, Blast_Score=223, Evalue=9e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 36669; Mature: 36538

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIATEPYMNTNTAVSFSSEHHLNSPEQKVFWQQVTQSSFKTRDDISLAYARITHPNSIG
CCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCE
TIVISSGRVESYLKYKELMFDLYQQGYSLFAIDHRGQGLSARTTANPHQGHIDKFQRYVD
EEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHH
DFAEFVTKIVKPHAMENYYLVGHSMGGAIGTLYLSQYPATFKAAVFSAPMYGIKLPLPKA
HHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHCCCCEEECCCHHH
FIRGLASLLDSSKQPNYILGGKGYHAEPFEKNDLTHSKLRYDDYRELYRQQPQLQLGSPT
HHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHHCCCCEEECCCC
NHWLVESIDAAEQAVDAARNSTIPLLILQAEEDTIVANAAQNRAVNGQCRLIKIPYARHE
CCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECHHCCCCCCCEEEEECCCHHHH
IFMERDKPRNLALNALCEFLKQIN
HHHCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SIATEPYMNTNTAVSFSSEHHLNSPEQKVFWQQVTQSSFKTRDDISLAYARITHPNSIG
CCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCE
TIVISSGRVESYLKYKELMFDLYQQGYSLFAIDHRGQGLSARTTANPHQGHIDKFQRYVD
EEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHH
DFAEFVTKIVKPHAMENYYLVGHSMGGAIGTLYLSQYPATFKAAVFSAPMYGIKLPLPKA
HHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHCCCCEEECCCHHH
FIRGLASLLDSSKQPNYILGGKGYHAEPFEKNDLTHSKLRYDDYRELYRQQPQLQLGSPT
HHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHHCCCCEEECCCC
NHWLVESIDAAEQAVDAARNSTIPLLILQAEEDTIVANAAQNRAVNGQCRLIKIPYARHE
CCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECHHCCCCCCCEEEEECCCHHHH
IFMERDKPRNLALNALCEFLKQIN
HHHCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]