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The map label for this gene is atoC [H]

Identifier: 157959915

GI number: 157959915

Start: 94655

End: 95962

Strand: Reverse

Name: atoC [H]

Synonym: Spea_0085

Alternate gene names: 157959915

Gene position: 95962-94655 (Counterclockwise)

Preceding gene: 157959916

Following gene: 157959914

Centisome position: 1.85

GC content: 48.55

Gene sequence:

>1308_bases
ATGAATCCTTCTAGTTTAAGATTTGTCGATGCAGATCTAACCCTATCTTGCCAAAATAGACTGGTTCAACAGGGCTTTAA
TTTATACTTGCCTGATGATAAAGATAGCCCTTGGTTAAGTTTGATCAACCTTTCTCATTGCGCGAGTCAGGATGTACCCG
TACGGCTACGACAATTTCCAGGTAAGCACCAAGTCGCCCTGCTCAATCCAGAGCAAACCGAGCTTGCCAGTATCGCCATG
CAGTGTGGTGTACAAGATTACCTGTTACTGCCAATTGATGCAGAGCAACTGTGCTCACTACTACAAAGATTACGTCGTTT
AGAACTGCCCAATAATGAGTTAATTTGCGCGGCTCCGGTTAGCCGCCAGCTGCTAATGCTAGCTCACCGAGCTGCGAGCA
CAGAGGCCACAGTACTACTCACCGGCGAAAGCGGCACAGGTAAAGAGCCGCTAGCTCGTTATATTCACCGCCATTCCAAC
CGTGCCGATAAGGCTTTTATCGCAATCAACTGCGCAGCTATTCCTGAAAGCATTCTAGAATCTATCCTGTTTGGTCACAT
CAAGGGGGCATTTACGGGTGCCACAACCGACCAGCCCGGTAAGTTCGAACTCGCCAACGGCGGAACGTTACTGCTCGATG
AAATCGGTGAGATGCCTCTGCTTTTACAAGCCAAACTACTGCGTGTACTGCAAGAGAGAGAGGTTGAGCGATTAGGCAGT
CACAGGTCAATTGCGTTAGATATTCGGGTCATTGCCGCCACCAACAAAGATCTGCGTCAAGCGGTGCAAGATGGCAAATT
CAGAGAAGACCTCTTCTATCGCTTAGATGTACTGCCGATAAAGATATTACCACTGCGTCAGAGACGCGAAGACATTCTGC
CCATTGCCGAACATTTTCTACAGCGATACAAAATTCTTGCGGCAAATCAGCAATGCTACTTCAGTGAGCAGGCTCGAAAC
TTACTGTTAAGCCACAACTGGCCCGGCAATGTGCGCGAACTAGAAAATACCATCCAACGCGCGCTGGTTATGCGACGTGG
TCAAGCATTACAAGCCGAAGATCTCGGCCTCGAAAATCAAGACGGTTCAATCTTAGTAGAACAAAATGAGTTAGGGCTTA
AGGCCTCTAAACGTCAGGCTGAGTTCCAATACATTATTGACACGCTTAAGCGCCACAACGGCCATAGGATCCAAACGGCT
GAGGCCTTGGGAATGACGACACGAGCATTGCGATACAAGCTGGCTCAAATGCGAGAAGAAGGGATCGATATTGAACGCCT
CCTCGCTCAAGCAGGTCATGCTGCGTAA

Upstream 100 bases:

>100_bases
AGCAACAACAGCACTCAAGGCCGAGAGCTAAACCGTCGAGTCACTTTGCGCTTAGTCAAATCTGCAGCAAATTCTGTGAG
CCCAGCTTCGAGAGCACAAA

Downstream 100 bases:

>100_bases
TCATAGTCTAGATCTATCAAGAGAATTTATATGTCAAATGCCAATATAGCCAGTGCTCAGTTAATGATGCAACAGCTCAA
CATCCATTCAGAGATGGCAA

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: Ornithine decarboxylase antizyme; Ornithine/arginine decarboxylase inhibitor [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MNPSSLRFVDADLTLSCQNRLVQQGFNLYLPDDKDSPWLSLINLSHCASQDVPVRLRQFPGKHQVALLNPEQTELASIAM
QCGVQDYLLLPIDAEQLCSLLQRLRRLELPNNELICAAPVSRQLLMLAHRAASTEATVLLTGESGTGKEPLARYIHRHSN
RADKAFIAINCAAIPESILESILFGHIKGAFTGATTDQPGKFELANGGTLLLDEIGEMPLLLQAKLLRVLQEREVERLGS
HRSIALDIRVIAATNKDLRQAVQDGKFREDLFYRLDVLPIKILPLRQRREDILPIAEHFLQRYKILAANQQCYFSEQARN
LLLSHNWPGNVRELENTIQRALVMRRGQALQAEDLGLENQDGSILVEQNELGLKASKRQAEFQYIIDTLKRHNGHRIQTA
EALGMTTRALRYKLAQMREEGIDIERLLAQAGHAA

Sequences:

>Translated_435_residues
MNPSSLRFVDADLTLSCQNRLVQQGFNLYLPDDKDSPWLSLINLSHCASQDVPVRLRQFPGKHQVALLNPEQTELASIAM
QCGVQDYLLLPIDAEQLCSLLQRLRRLELPNNELICAAPVSRQLLMLAHRAASTEATVLLTGESGTGKEPLARYIHRHSN
RADKAFIAINCAAIPESILESILFGHIKGAFTGATTDQPGKFELANGGTLLLDEIGEMPLLLQAKLLRVLQEREVERLGS
HRSIALDIRVIAATNKDLRQAVQDGKFREDLFYRLDVLPIKILPLRQRREDILPIAEHFLQRYKILAANQQCYFSEQARN
LLLSHNWPGNVRELENTIQRALVMRRGQALQAEDLGLENQDGSILVEQNELGLKASKRQAEFQYIIDTLKRHNGHRIQTA
EALGMTTRALRYKLAQMREEGIDIERLLAQAGHAA
>Mature_435_residues
MNPSSLRFVDADLTLSCQNRLVQQGFNLYLPDDKDSPWLSLINLSHCASQDVPVRLRQFPGKHQVALLNPEQTELASIAM
QCGVQDYLLLPIDAEQLCSLLQRLRRLELPNNELICAAPVSRQLLMLAHRAASTEATVLLTGESGTGKEPLARYIHRHSN
RADKAFIAINCAAIPESILESILFGHIKGAFTGATTDQPGKFELANGGTLLLDEIGEMPLLLQAKLLRVLQEREVERLGS
HRSIALDIRVIAATNKDLRQAVQDGKFREDLFYRLDVLPIKILPLRQRREDILPIAEHFLQRYKILAANQQCYFSEQARN
LLLSHNWPGNVRELENTIQRALVMRRGQALQAEDLGLENQDGSILVEQNELGLKASKRQAEFQYIIDTLKRHNGHRIQTA
EALGMTTRALRYKLAQMREEGIDIERLLAQAGHAA

Specific function: Member of the two-component regulatory system AtoS/AtoC involved in the transcriptional regulation of the ato genes for acetoacetate metabolism. Also an inhibitor of polyamine biosynthesis [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=374, Percent_Identity=45.7219251336898, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1790437, Length=365, Percent_Identity=41.0958904109589, Blast_Score=261, Evalue=5e-71,
Organism=Escherichia coli, GI87082117, Length=310, Percent_Identity=47.4193548387097, Blast_Score=258, Evalue=7e-70,
Organism=Escherichia coli, GI1790299, Length=380, Percent_Identity=39.4736842105263, Blast_Score=244, Evalue=7e-66,
Organism=Escherichia coli, GI1789233, Length=317, Percent_Identity=43.217665615142, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1789087, Length=340, Percent_Identity=42.9411764705882, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=48.936170212766, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI1788905, Length=353, Percent_Identity=40.5099150141643, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1786524, Length=235, Percent_Identity=48.936170212766, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1787583, Length=313, Percent_Identity=37.0607028753994, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI87081872, Length=219, Percent_Identity=44.7488584474886, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1789828, Length=219, Percent_Identity=39.7260273972603, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI87081858, Length=315, Percent_Identity=31.4285714285714, Blast_Score=126, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48954; Mature: 48954

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPSSLRFVDADLTLSCQNRLVQQGFNLYLPDDKDSPWLSLINLSHCASQDVPVRLRQFP
CCCCCCEEEECCEEEHHHHHHHHCCCEEECCCCCCCCHHHHEEHHHHCCCCCCHHHHHCC
GKHQVALLNPEQTELASIAMQCGVQDYLLLPIDAEQLCSLLQRLRRLELPNNELICAAPV
CCCEEEEECCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEEECCH
SRQLLMLAHRAASTEATVLLTGESGTGKEPLARYIHRHSNRADKAFIAINCAAIPESILE
HHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHH
SILFGHIKGAFTGATTDQPGKFELANGGTLLLDEIGEMPLLLQAKLLRVLQEREVERLGS
HHHHHHHCCCCCCCCCCCCCCEEECCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHCCC
HRSIALDIRVIAATNKDLRQAVQDGKFREDLFYRLDVLPIKILPLRQRREDILPIAEHFL
CCEEEEEEEEEEECCHHHHHHHHCCCHHHHHHHHHEECEEEEECCHHHHHHHHHHHHHHH
QRYKILAANQQCYFSEQARNLLLSHNWPGNVRELENTIQRALVMRRGQALQAEDLGLENQ
HHHHHHHCCCCCEEHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCC
DGSILVEQNELGLKASKRQAEFQYIIDTLKRHNGHRIQTAEALGMTTRALRYKLAQMREE
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
GIDIERLLAQAGHAA
CCCHHHHHHHCCCCC
>Mature Secondary Structure
MNPSSLRFVDADLTLSCQNRLVQQGFNLYLPDDKDSPWLSLINLSHCASQDVPVRLRQFP
CCCCCCEEEECCEEEHHHHHHHHCCCEEECCCCCCCCHHHHEEHHHHCCCCCCHHHHHCC
GKHQVALLNPEQTELASIAMQCGVQDYLLLPIDAEQLCSLLQRLRRLELPNNELICAAPV
CCCEEEEECCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEEECCH
SRQLLMLAHRAASTEATVLLTGESGTGKEPLARYIHRHSNRADKAFIAINCAAIPESILE
HHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHH
SILFGHIKGAFTGATTDQPGKFELANGGTLLLDEIGEMPLLLQAKLLRVLQEREVERLGS
HHHHHHHCCCCCCCCCCCCCCEEECCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHCCC
HRSIALDIRVIAATNKDLRQAVQDGKFREDLFYRLDVLPIKILPLRQRREDILPIAEHFL
CCEEEEEEEEEEECCHHHHHHHHCCCHHHHHHHHHEECEEEEECCHHHHHHHHHHHHHHH
QRYKILAANQQCYFSEQARNLLLSHNWPGNVRELENTIQRALVMRRGQALQAEDLGLENQ
HHHHHHHCCCCCEEHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCC
DGSILVEQNELGLKASKRQAEFQYIIDTLKRHNGHRIQTAEALGMTTRALRYKLAQMREE
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
GIDIERLLAQAGHAA
CCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 8346225 [H]