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The map label for this gene is epsN [H]

Identifier: 157959877

GI number: 157959877

Start: 62895

End: 64055

Strand: Direct

Name: epsN [H]

Synonym: Spea_0047

Alternate gene names: 157959877

Gene position: 62895-64055 (Clockwise)

Preceding gene: 157959876

Following gene: 157959878

Centisome position: 1.22

GC content: 44.1

Gene sequence:

>1161_bases
ATGAAAGCGACACCAATTGTTGAGTTCGTTCGAGACATATACAAAACTGACGATTTTATTCCATTGCACGTACCGACGTT
TAATGGCAACGAAAAAGCTTATGTTATGCAAACTATCGAAAGCACTTTTGTTTCTAGTGTAGGTAAGTTTGTTGATGATT
TCGAACGAAAGATAGAGGCTTATACTGGTTCTGCTAAGGCGGTAGCGACAGTGAACGGTACAGCAGCCTTGCATGCGGCA
CTGTACATGGCTGGTGTTCAGCGTGATGATTTAGTTATTACACAAGCGTTGACCTTTGTCGCAACCTGCAATGCCTTGCA
TCATATGGGGGCTGAACCCATCTTTGTCGATGTTTCTCCTATTAGCTTGGGACTTTGTCCAAAAGCTGTGGAGGCTTACC
TGTGCGAGTATGCAGAGAAAACAGAAACAGGTTGTGTTCACAAAGGCAGCGGGCAACGAATCAAAGCTGTCGTGCCGATG
CATACATTTGGCCATCCCGTCGAATTAGATGAACTTAATATTGTTTGTCGGCAGTGGAATATTACTTTAGTTGAAGATGC
CGCTGAAAGCTTAGGCTCTTTTTACAAAGGCAAGCATACCGGCACTATTGGTGATTTTGGTGCCCTGAGTTTTAATGGTA
ATAAAATTATCACCACCGGCGGTGGCGGCATGGTGTTATGCCAAACGGCAGAGTTTGGGGCGCGTACTAAACACGTGACG
ACCACAGCAAAAGTGCCGCATCCGTATGAATTCTTCCATGATGAGCCTGGCTTTAACTACCGCATGCCTAATCTCAATGC
TGCGTTAGGTTGTGCGCAAATGGAAGCGCTAGAGAATTACCTGATTCAAAAGAGACAGCTTGCTGGGTTATATGAAGGCT
TTTTCAGCGGTTCAGATTTTAAGTTTGTAACTGAGCCAGGTTATGCACGGTCAAATTACTGGTTAAACGCGGTAATTTGC
CCGGATAAGCAGAGCAGAGAGGAAGTGCTCTCTGTTACAAATAATTCAGGTGTGATGACTCGTCCAATTTGGCAATTAAT
GCATAGATTGCCAATGTTTAAAAATGCAATGCGTGGTGATCTCTCCTGCTCAGAGTTTGTCGAAGCTCATTTAATAAATC
TTCCAAGTACACCCGTTGTACTAAATACCTCGCAGGGATAA

Upstream 100 bases:

>100_bases
TAAAGAAGATATCGTTAAATTATTCTTCACCATGCTGCCTGAATTTGCTCATAAAGAAACTGGTAAATATCTAGATAGTA
AGATGTAAGCGGAGTACGTG

Downstream 100 bases:

>100_bases
CTAGAATCATGACGCAGATGCATAAAAAAGTTGCGGTTTTTACAGGGACAAGAGCTGAGTATGGTCTGCTCTATTGGCTT
TTAAAGGACATACAAAGCGA

Product: DegT/DnrJ/EryC1/StrS aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MKATPIVEFVRDIYKTDDFIPLHVPTFNGNEKAYVMQTIESTFVSSVGKFVDDFERKIEAYTGSAKAVATVNGTAALHAA
LYMAGVQRDDLVITQALTFVATCNALHHMGAEPIFVDVSPISLGLCPKAVEAYLCEYAEKTETGCVHKGSGQRIKAVVPM
HTFGHPVELDELNIVCRQWNITLVEDAAESLGSFYKGKHTGTIGDFGALSFNGNKIITTGGGGMVLCQTAEFGARTKHVT
TTAKVPHPYEFFHDEPGFNYRMPNLNAALGCAQMEALENYLIQKRQLAGLYEGFFSGSDFKFVTEPGYARSNYWLNAVIC
PDKQSREEVLSVTNNSGVMTRPIWQLMHRLPMFKNAMRGDLSCSEFVEAHLINLPSTPVVLNTSQG

Sequences:

>Translated_386_residues
MKATPIVEFVRDIYKTDDFIPLHVPTFNGNEKAYVMQTIESTFVSSVGKFVDDFERKIEAYTGSAKAVATVNGTAALHAA
LYMAGVQRDDLVITQALTFVATCNALHHMGAEPIFVDVSPISLGLCPKAVEAYLCEYAEKTETGCVHKGSGQRIKAVVPM
HTFGHPVELDELNIVCRQWNITLVEDAAESLGSFYKGKHTGTIGDFGALSFNGNKIITTGGGGMVLCQTAEFGARTKHVT
TTAKVPHPYEFFHDEPGFNYRMPNLNAALGCAQMEALENYLIQKRQLAGLYEGFFSGSDFKFVTEPGYARSNYWLNAVIC
PDKQSREEVLSVTNNSGVMTRPIWQLMHRLPMFKNAMRGDLSCSEFVEAHLINLPSTPVVLNTSQG
>Mature_386_residues
MKATPIVEFVRDIYKTDDFIPLHVPTFNGNEKAYVMQTIESTFVSSVGKFVDDFERKIEAYTGSAKAVATVNGTAALHAA
LYMAGVQRDDLVITQALTFVATCNALHHMGAEPIFVDVSPISLGLCPKAVEAYLCEYAEKTETGCVHKGSGQRIKAVVPM
HTFGHPVELDELNIVCRQWNITLVEDAAESLGSFYKGKHTGTIGDFGALSFNGNKIITTGGGGMVLCQTAEFGARTKHVT
TTAKVPHPYEFFHDEPGFNYRMPNLNAALGCAQMEALENYLIQKRQLAGLYEGFFSGSDFKFVTEPGYARSNYWLNAVIC
PDKQSREEVLSVTNNSGVMTRPIWQLMHRLPMFKNAMRGDLSCSEFVEAHLINLPSTPVVLNTSQG

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI145693159, Length=339, Percent_Identity=25.9587020648968, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI2367285, Length=288, Percent_Identity=29.8611111111111, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 42445; Mature: 42445

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKATPIVEFVRDIYKTDDFIPLHVPTFNGNEKAYVMQTIESTFVSSVGKFVDDFERKIEA
CCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
YTGSAKAVATVNGTAALHAALYMAGVQRDDLVITQALTFVATCNALHHMGAEPIFVDVSP
HCCCCEEEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECC
ISLGLCPKAVEAYLCEYAEKTETGCVHKGSGQRIKAVVPMHTFGHPVELDELNIVCRQWN
CEECCCHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEECCHHCCCCCEECCCEEEEEECC
ITLVEDAAESLGSFYKGKHTGTIGDFGALSFNGNKIITTGGGGMVLCQTAEFGARTKHVT
EEEHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEECCCCEEEEEECCCCCCCCEEE
TTAKVPHPYEFFHDEPGFNYRMPNLNAALGCAQMEALENYLIQKRQLAGLYEGFFSGSDF
EECCCCCCHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
KFVTEPGYARSNYWLNAVICPDKQSREEVLSVTNNSGVMTRPIWQLMHRLPMFKNAMRGD
EEEECCCCCCCCCEEEEEECCCCCCHHHHHEECCCCCCEEHHHHHHHHHCHHHHHHHCCC
LSCSEFVEAHLINLPSTPVVLNTSQG
CCHHHHHHHHHCCCCCCCEEEECCCC
>Mature Secondary Structure
MKATPIVEFVRDIYKTDDFIPLHVPTFNGNEKAYVMQTIESTFVSSVGKFVDDFERKIEA
CCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
YTGSAKAVATVNGTAALHAALYMAGVQRDDLVITQALTFVATCNALHHMGAEPIFVDVSP
HCCCCEEEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECC
ISLGLCPKAVEAYLCEYAEKTETGCVHKGSGQRIKAVVPMHTFGHPVELDELNIVCRQWN
CEECCCHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEECCHHCCCCCEECCCEEEEEECC
ITLVEDAAESLGSFYKGKHTGTIGDFGALSFNGNKIITTGGGGMVLCQTAEFGARTKHVT
EEEHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEECCCCEEEEEECCCCCCCCEEE
TTAKVPHPYEFFHDEPGFNYRMPNLNAALGCAQMEALENYLIQKRQLAGLYEGFFSGSDF
EECCCCCCHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
KFVTEPGYARSNYWLNAVICPDKQSREEVLSVTNNSGVMTRPIWQLMHRLPMFKNAMRGD
EEEECCCCCCCCCEEEEEECCCCCCHHHHHEECCCCCCEEHHHHHHHHHCHHHHHHHCCC
LSCSEFVEAHLINLPSTPVVLNTSQG
CCHHHHHHHHHCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]