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The map label for this gene is glyS [H]

Identifier: 157959836

GI number: 157959836

Start: 7582

End: 9651

Strand: Reverse

Name: glyS [H]

Synonym: Spea_0006

Alternate gene names: 157959836

Gene position: 9651-7582 (Counterclockwise)

Preceding gene: 157959837

Following gene: NA

Centisome position: 0.19

GC content: 46.14

Gene sequence:

>2070_bases
ATGAATTTTGAAAACTTACTGATTGAAATCGGTACTGAAGAGTTACCTCCAAAATCACTGCGTAAACTGGCTGAGTCTTT
CTTAGCTAATTTTACCGAAGAGCTAACAAAAGCAGAGCTAAGCTTTGAGTCTGCTGTATGGCACGCTGCGCCACGTCGTC
TAGCTATCTGCATTAATCAACTTGCACTGGCTCAGGCTGACAAAGTAGTTGAGAAACGCGGACCCGCTGTAGCACAAGCT
TTCGATGCCGATGGTAATCCAACTAAAGCCGCTATGGGCTGGGCCCGTGGTAACGGTATTACAGTTGAGCAAGCTGAGCG
TTTAAAGACAGATAAAGGCGAATGGTTACTACACCAAGCACGTGTAGTCGGTGTTGAGACTAAGAGCCTAATTGCCGATA
TGGCGCAACGGTCTTTAGACAAATTACCTATTCCAAAGCCAATGCGTTGGGGTAGCAATACCACGCAATTTATCCGTCCT
GTTCACACAGTTACCATGTTACTTGGTAGTGAAGTGGTTGACGGTGAGCTACTAGGCATCAAGTCTGATCGCATTATTCG
TGGTCACCGCTTTATGGGTGAGTCTAGCTTCAAGCTAGATCATGCCGACAACTACCTTGTTGCGCTAAAAGAAAAAGGTA
AGGTACTAGCGGATTATGAAGCGCGTAAGGCAATCATCAAGACTGATGCCGAAGCTGCAGCAGCTAAAATTGGTGGCGTA
GCCGATTTAGAAGATGACCTACTTGAAGAAGTGACCTCTTTGGTTGAATGGCCAGTAGTACTAACGGCAAGCTTCGAAGA
GAAGTTCTTAGATGTGCCAGCTGAAGCCTTGGTTTACACCATGAAAGGCGACCAAAAATACTTCCCAGTATTTGATAACG
CTGGTCAGTTACTGCCTAACTTTATCTTCGTAACTAACATCGAATCTAAAGATCCACAGCAGATTATTGCCGGTAACGAG
AAAGTGGTTCGCCCACGTCTCGCCGATGCTGAGTTCTTCTTCGAAACAGATAAGAAAGACACACTTGAAGCTCGCCTTAC
TAGCCTAGAAACCGTTGTATTCCAAAAGCAGCTTGGCACTATTAAGCAACGTGTTGAACGTATCTCAGCTATGGCTGGTT
ACATTGCCACTAGCATCGATGCTAACAGCGAAGAAGCGGCGCGTGCAGGTCTATTATCTAAGTCAGATTTGATGACTAAC
ATGGTAATGGAGTTTACTGACCTTCAAGGCACCATGGGTATGCATTACGCCCGCCTAAATGGTGAAACGGAAGCGGTAGC
CGTAGCTCTGTCTGAGCAATATAAGCCTAAGTTCTCTGGTGATACCGTACCAACAGCACCTATCTCTATCTGTGTTGCAC
TCGCTGAAAAACTAGACACGCTAGTGGGTATCTTTGGTATTGGCCAAGCGCCTAAAGGTGCTGCGGATCCATTCGCACTT
CGCCGTGCAGCAATTGGTGTGCTACGTATTTGTTTAGAGAACAACTTACCGCTAGATCTTGTTGATTTAATTGCTAAAGC
ACAAGAGCTACACGGAGAGAATCTCACTAACGCAGATGTTGCCGAGCAAGTACTTGAGTTCTTTATGGCTCGCTTCCGCG
CTTGGTATCAAGATCAAGGCGTGAGTGTAGACGTGATTCTAGCAGTATTAGCTCGTCGCCCTACCGCTCCTGCCGATTTT
GAAAGCCGCATTAAAGCTGTTGCTCACTTCAGAACTCTTGAACAAGCATCTGCACTTGCTGCTGCTAATAAGCGTGTATC
TAACATCTTGGCTAAAGTTGAAGGCGAGTTACCTGCTGCTATCGACGATAAGCTACTTGTTGAAGCCGCTGAAAAAGCGC
TGGCTGAAAAGCTTGCAGAATTGCAACCACAGCTAGCGCCACTATTTGCAGCCGCTAACTATCAAGAAGCATTAGCGTTA
CTAGCTAGCCTACGTGAAAGCGTTGATACCTTCTTCGAAGATGTGATGGTAATGGCTGATGATGAAGCGCTAAAGAACAA
CCGCCTAGCGCTACTATCTAGCCTGCGAGAGCAGTTCTTACATGCTGCAGATATTTCACTGCTTCAGTAA

Upstream 100 bases:

>100_bases
ATATCCTACGCGTTCGTACCATGGCTAAAGGCGTTGCAGAATCTTACTATCAGGCCCGTGAAGCTCTTGGCTTCCCAATG
TGTAAGTAGAGGAATAACAG

Downstream 100 bases:

>100_bases
CCACTGAATCGATGAATCATAAAAAACGCGCCATTTGGCGCGTTTTTTTATTGAGTAGTTATTAGCTGAATAAAGCTAAA
ACTGCACCAGCTTCTGATGC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 689; Mature: 689

Protein sequence:

>689_residues
MNFENLLIEIGTEELPPKSLRKLAESFLANFTEELTKAELSFESAVWHAAPRRLAICINQLALAQADKVVEKRGPAVAQA
FDADGNPTKAAMGWARGNGITVEQAERLKTDKGEWLLHQARVVGVETKSLIADMAQRSLDKLPIPKPMRWGSNTTQFIRP
VHTVTMLLGSEVVDGELLGIKSDRIIRGHRFMGESSFKLDHADNYLVALKEKGKVLADYEARKAIIKTDAEAAAAKIGGV
ADLEDDLLEEVTSLVEWPVVLTASFEEKFLDVPAEALVYTMKGDQKYFPVFDNAGQLLPNFIFVTNIESKDPQQIIAGNE
KVVRPRLADAEFFFETDKKDTLEARLTSLETVVFQKQLGTIKQRVERISAMAGYIATSIDANSEEAARAGLLSKSDLMTN
MVMEFTDLQGTMGMHYARLNGETEAVAVALSEQYKPKFSGDTVPTAPISICVALAEKLDTLVGIFGIGQAPKGAADPFAL
RRAAIGVLRICLENNLPLDLVDLIAKAQELHGENLTNADVAEQVLEFFMARFRAWYQDQGVSVDVILAVLARRPTAPADF
ESRIKAVAHFRTLEQASALAAANKRVSNILAKVEGELPAAIDDKLLVEAAEKALAEKLAELQPQLAPLFAAANYQEALAL
LASLRESVDTFFEDVMVMADDEALKNNRLALLSSLREQFLHAADISLLQ

Sequences:

>Translated_689_residues
MNFENLLIEIGTEELPPKSLRKLAESFLANFTEELTKAELSFESAVWHAAPRRLAICINQLALAQADKVVEKRGPAVAQA
FDADGNPTKAAMGWARGNGITVEQAERLKTDKGEWLLHQARVVGVETKSLIADMAQRSLDKLPIPKPMRWGSNTTQFIRP
VHTVTMLLGSEVVDGELLGIKSDRIIRGHRFMGESSFKLDHADNYLVALKEKGKVLADYEARKAIIKTDAEAAAAKIGGV
ADLEDDLLEEVTSLVEWPVVLTASFEEKFLDVPAEALVYTMKGDQKYFPVFDNAGQLLPNFIFVTNIESKDPQQIIAGNE
KVVRPRLADAEFFFETDKKDTLEARLTSLETVVFQKQLGTIKQRVERISAMAGYIATSIDANSEEAARAGLLSKSDLMTN
MVMEFTDLQGTMGMHYARLNGETEAVAVALSEQYKPKFSGDTVPTAPISICVALAEKLDTLVGIFGIGQAPKGAADPFAL
RRAAIGVLRICLENNLPLDLVDLIAKAQELHGENLTNADVAEQVLEFFMARFRAWYQDQGVSVDVILAVLARRPTAPADF
ESRIKAVAHFRTLEQASALAAANKRVSNILAKVEGELPAAIDDKLLVEAAEKALAEKLAELQPQLAPLFAAANYQEALAL
LASLRESVDTFFEDVMVMADDEALKNNRLALLSSLREQFLHAADISLLQ
>Mature_689_residues
MNFENLLIEIGTEELPPKSLRKLAESFLANFTEELTKAELSFESAVWHAAPRRLAICINQLALAQADKVVEKRGPAVAQA
FDADGNPTKAAMGWARGNGITVEQAERLKTDKGEWLLHQARVVGVETKSLIADMAQRSLDKLPIPKPMRWGSNTTQFIRP
VHTVTMLLGSEVVDGELLGIKSDRIIRGHRFMGESSFKLDHADNYLVALKEKGKVLADYEARKAIIKTDAEAAAAKIGGV
ADLEDDLLEEVTSLVEWPVVLTASFEEKFLDVPAEALVYTMKGDQKYFPVFDNAGQLLPNFIFVTNIESKDPQQIIAGNE
KVVRPRLADAEFFFETDKKDTLEARLTSLETVVFQKQLGTIKQRVERISAMAGYIATSIDANSEEAARAGLLSKSDLMTN
MVMEFTDLQGTMGMHYARLNGETEAVAVALSEQYKPKFSGDTVPTAPISICVALAEKLDTLVGIFGIGQAPKGAADPFAL
RRAAIGVLRICLENNLPLDLVDLIAKAQELHGENLTNADVAEQVLEFFMARFRAWYQDQGVSVDVILAVLARRPTAPADF
ESRIKAVAHFRTLEQASALAAANKRVSNILAKVEGELPAAIDDKLLVEAAEKALAEKLAELQPQLAPLFAAANYQEALAL
LASLRESVDTFFEDVMVMADDEALKNNRLALLSSLREQFLHAADISLLQ

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=689, Percent_Identity=64.5863570391872, Blast_Score=893, Evalue=0.0,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 75546; Mature: 75546

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFENLLIEIGTEELPPKSLRKLAESFLANFTEELTKAELSFESAVWHAAPRRLAICINQ
CCHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
LALAQADKVVEKRGPAVAQAFDADGNPTKAAMGWARGNGITVEQAERLKTDKGEWLLHQA
HHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHH
RVVGVETKSLIADMAQRSLDKLPIPKPMRWGSNTTQFIRPVHTVTMLLGSEVVDGELLGI
HHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHCCCEEEEC
KSDRIIRGHRFMGESSFKLDHADNYLVALKEKGKVLADYEARKAIIKTDAEAAAAKIGGV
CCCCEEECHHCCCCCCCEEECCCCEEEEEECCCCEEECHHHHHHHHHCCHHHHHHHHCCC
ADLEDDLLEEVTSLVEWPVVLTASFEEKFLDVPAEALVYTMKGDQKYFPVFDNAGQLLPN
CCCHHHHHHHHHHHHHCCEEEEECHHHHHHCCCHHHEEEEECCCCEECCCCCCCHHHCCC
FIFVTNIESKDPQQIIAGNEKVVRPRLADAEFFFETDKKDTLEARLTSLETVVFQKQLGT
EEEEEECCCCCCHHHHCCCCCEECCCCCCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHH
IKQRVERISAMAGYIATSIDANSEEAARAGLLSKSDLMTNMVMEFTDLQGTMGMHYARLN
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCHHCCEEEECC
GETEAVAVALSEQYKPKFSGDTVPTAPISICVALAEKLDTLVGIFGIGQAPKGAADPFAL
CCCHHEEEEHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAAIGVLRICLENNLPLDLVDLIAKAQELHGENLTNADVAEQVLEFFMARFRAWYQDQG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
VSVDVILAVLARRPTAPADFESRIKAVAHFRTLEQASALAAANKRVSNILAKVEGELPAA
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IDDKLLVEAAEKALAEKLAELQPQLAPLFAAANYQEALALLASLRESVDTFFEDVMVMAD
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
DEALKNNRLALLSSLREQFLHAADISLLQ
CHHHHCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFENLLIEIGTEELPPKSLRKLAESFLANFTEELTKAELSFESAVWHAAPRRLAICINQ
CCHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
LALAQADKVVEKRGPAVAQAFDADGNPTKAAMGWARGNGITVEQAERLKTDKGEWLLHQA
HHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHH
RVVGVETKSLIADMAQRSLDKLPIPKPMRWGSNTTQFIRPVHTVTMLLGSEVVDGELLGI
HHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHCCCEEEEC
KSDRIIRGHRFMGESSFKLDHADNYLVALKEKGKVLADYEARKAIIKTDAEAAAAKIGGV
CCCCEEECHHCCCCCCCEEECCCCEEEEEECCCCEEECHHHHHHHHHCCHHHHHHHHCCC
ADLEDDLLEEVTSLVEWPVVLTASFEEKFLDVPAEALVYTMKGDQKYFPVFDNAGQLLPN
CCCHHHHHHHHHHHHHCCEEEEECHHHHHHCCCHHHEEEEECCCCEECCCCCCCHHHCCC
FIFVTNIESKDPQQIIAGNEKVVRPRLADAEFFFETDKKDTLEARLTSLETVVFQKQLGT
EEEEEECCCCCCHHHHCCCCCEECCCCCCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHH
IKQRVERISAMAGYIATSIDANSEEAARAGLLSKSDLMTNMVMEFTDLQGTMGMHYARLN
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCHHCCEEEECC
GETEAVAVALSEQYKPKFSGDTVPTAPISICVALAEKLDTLVGIFGIGQAPKGAADPFAL
CCCHHEEEEHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAAIGVLRICLENNLPLDLVDLIAKAQELHGENLTNADVAEQVLEFFMARFRAWYQDQG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
VSVDVILAVLARRPTAPADFESRIKAVAHFRTLEQASALAAANKRVSNILAKVEGELPAA
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IDDKLLVEAAEKALAEKLAELQPQLAPLFAAANYQEALALLASLRESVDTFFEDVMVMAD
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
DEALKNNRLALLSSLREQFLHAADISLLQ
CHHHHCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA