Click here to switch to the map view.

The map label for this gene is amiD [H]

Identifier: 157826369

GI number: 157826369

Start: 1217748

End: 1218494

Strand: Reverse

Name: amiD [H]

Synonym: A1C_06790

Alternate gene names: 157826369

Gene position: 1218494-1217748 (Counterclockwise)

Preceding gene: 157826370

Following gene: 157826368

Centisome position: 98.98

GC content: 31.86

Gene sequence:

>747_bases
ATGATCGTAATCGATAAAATATCTTATAGATCTGTGGGGTTTGATAAACGTATTAAATTCCTAGTGCTACATTATACACA
GTGTGATTTTAAGCAGTCTTTAGATTTTCTAACCGGTGAAAAGTTAAGTAGCCATTATTTAATAAACAATGAAAACCCTG
AACATATATTTCAATTAGTAGAAGAGCATGATAGAGCATGGCATGCTGGAGTAAGTTACTGGCAAGGTCATGAGCGTATT
AATGATACATCAATTGGTATTGAAATCGTAAATCCTGCATTTGAAATAAATACAAAAAATAATAATATAGTTTGGATACC
ATATGCGGAAAGGCAAATTAATAGTGTCATAAGTTTGTGTAAACAGATTATCGCTAAATATGATATTAAACCTACTAGAG
TGGTCGGGCATTCTGATATTGCTCCAGGTAGAAAACAGGATCCTGGTCCGCTATTTCCTTGGAAACTATTATATAATAAC
GATATTGGAGCATGGTATGATGAACAAGTGTTTAACGATCTATTGCCGCAAATCGACATAACCAATATTGCAGCAATTCA
GCAAAAATTTATTACATACGGTTATAAGCTTGAAGCTACTGGAATTTTAGATTCTAAAATGAAAGATGTTATAATTTCGT
TTCAAATGCATTTTCGTCCTAGTAATTTTTCCGGCGATTTGGATGCTGAAACCATAGCAATTTTAGATGCATTAATATTG
AAATATAAATCTGAGTTAAGTAATTAA

Upstream 100 bases:

>100_bases
CTGTATTTATATTTTTTTCTAGATTCCTGCTTCTAGCTAGGAATGACATAAGAGCAATGCAATAAAGCCGTTTTTCACTA
AGATTAACATCTTTTAAACT

Downstream 100 bases:

>100_bases
TATGACACATTTATAATATGCTACAATTTATAACTATACCGAATCTTATGGATTATCGGGTTACTTTGAAATTAATGGAA
GATTACGTTAATAAAGTAAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MIVIDKISYRSVGFDKRIKFLVLHYTQCDFKQSLDFLTGEKLSSHYLINNENPEHIFQLVEEHDRAWHAGVSYWQGHERI
NDTSIGIEIVNPAFEINTKNNNIVWIPYAERQINSVISLCKQIIAKYDIKPTRVVGHSDIAPGRKQDPGPLFPWKLLYNN
DIGAWYDEQVFNDLLPQIDITNIAAIQQKFITYGYKLEATGILDSKMKDVIISFQMHFRPSNFSGDLDAETIAILDALIL
KYKSELSN

Sequences:

>Translated_248_residues
MIVIDKISYRSVGFDKRIKFLVLHYTQCDFKQSLDFLTGEKLSSHYLINNENPEHIFQLVEEHDRAWHAGVSYWQGHERI
NDTSIGIEIVNPAFEINTKNNNIVWIPYAERQINSVISLCKQIIAKYDIKPTRVVGHSDIAPGRKQDPGPLFPWKLLYNN
DIGAWYDEQVFNDLLPQIDITNIAAIQQKFITYGYKLEATGILDSKMKDVIISFQMHFRPSNFSGDLDAETIAILDALIL
KYKSELSN
>Mature_248_residues
MIVIDKISYRSVGFDKRIKFLVLHYTQCDFKQSLDFLTGEKLSSHYLINNENPEHIFQLVEEHDRAWHAGVSYWQGHERI
NDTSIGIEIVNPAFEINTKNNNIVWIPYAERQINSVISLCKQIIAKYDIKPTRVVGHSDIAPGRKQDPGPLFPWKLLYNN
DIGAWYDEQVFNDLLPQIDITNIAAIQQKFITYGYKLEATGILDSKMKDVIISFQMHFRPSNFSGDLDAETIAILDALIL
KYKSELSN

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=236, Percent_Identity=45.7627118644068, Blast_Score=197, Evalue=6e-52,
Organism=Escherichia coli, GI1786300, Length=114, Percent_Identity=38.5964912280702, Blast_Score=77, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 28593; Mature: 28593

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVIDKISYRSVGFDKRIKFLVLHYTQCDFKQSLDFLTGEKLSSHYLINNENPEHIFQLV
CEEEECCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHH
EEHDRAWHAGVSYWQGHERINDTSIGIEIVNPAFEINTKNNNIVWIPYAERQINSVISLC
HHHHHHHHHCCHHHCCCCCCCCCEEEEEEECCEEEEECCCCCEEEEECHHHHHHHHHHHH
KQIIAKYDIKPTRVVGHSDIAPGRKQDPGPLFPWKLLYNNDIGAWYDEQVFNDLLPQIDI
HHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCC
TNIAAIQQKFITYGYKLEATGILDSKMKDVIISFQMHFRPSNFSGDLDAETIAILDALIL
HHHHHHHHHHHHCCEEEEECCCHHHHHHHHEEEEEEEEECCCCCCCCCHHHHHHHHHHHH
KYKSELSN
HHHHHHCC
>Mature Secondary Structure
MIVIDKISYRSVGFDKRIKFLVLHYTQCDFKQSLDFLTGEKLSSHYLINNENPEHIFQLV
CEEEECCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHH
EEHDRAWHAGVSYWQGHERINDTSIGIEIVNPAFEINTKNNNIVWIPYAERQINSVISLC
HHHHHHHHHCCHHHCCCCCCCCCEEEEEEECCEEEEECCCCCEEEEECHHHHHHHHHHHH
KQIIAKYDIKPTRVVGHSDIAPGRKQDPGPLFPWKLLYNNDIGAWYDEQVFNDLLPQIDI
HHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCC
TNIAAIQQKFITYGYKLEATGILDSKMKDVIISFQMHFRPSNFSGDLDAETIAILDALIL
HHHHHHHHHHHHCCEEEEECCCHHHHHHHHEEEEEEEEECCCCCCCCCHHHHHHHHHHHH
KYKSELSN
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]