Click here to switch to the map view.

The map label for this gene is metG [H]

Identifier: 157826102

GI number: 157826102

Start: 948688

End: 950226

Strand: Reverse

Name: metG [H]

Synonym: A1C_05330

Alternate gene names: 157826102

Gene position: 950226-948688 (Counterclockwise)

Preceding gene: 157826103

Following gene: 157826101

Centisome position: 77.19

GC content: 36.0

Gene sequence:

>1539_bases
ATGAATAATACTTACTACATTACTACCCCTATATATTACGTTAACGATGTTCCTCATATCGGTCATGCTTATACTAGCGT
TGCAAGTGATGTAATCGCTCGTTTCATGCGCCTTCGTGGTCTCGACGTAATATTTTTAACAGGTACCGATGAACATGGGC
AGAAAGTAGAAAAAGCAGCAATTAACAAAAATATTGATCCTCAAAAATTTACCGATCAGACCTCGGAGAGTTTTCGTCAT
CTGATGGCTGTTATGCATATTTCTAATGATGATTTTATTAGGACCACGGAAAATAGACATAAAGAAGCTGTAGCTGTTTT
TTGGCAGAAATTGCTAGATAACGGTACAATCTATGAAGGATTTTATGAAGGTTGGTATGCAGTGCGAGATGAGGCTTTTT
TTGATGAGTCGGAACTTACGAGTGACGGTTTAGCGCCGACGGGTGCTCCAGTTGAATGGGTTAAAGAACCAAGCTATTTC
TTTAATCTTTCACAATGGCAAGATAAATTGCTTGAGTTTTATGAAGCAAACCCTGATTTTATCAGACCTATATCAAGGCG
TAACGAAGTAATTAGCTTTGTTAAATCAGGTCTAAAAGATTTATCCGTGTCACGTACTACTTTTAACTGGGGTATAAAAG
TTCCAAATAACGAAAAACACGTGATTTATGTTTGGCTTGATGCACTTGCTAATTATATTTCGGCACTCGGTTATCCTGAT
GAACAAAGTAGTTACGGTAAATTTTGGCCGGCTGATTTACACGTAGTAGGTAAGGATATCTTGCGTTTTCATACTGTCTA
TTGGCCTGCTTTTTTGATGGCTGCAGAAATTCCGCTACCGAAAACAATTATGGCTCATGGTTGGTGGACGAATGAGGGGC
AGAAAATCTCTAAATCTCTTGGTAATACCATTGATCCGATTAAGTTAATCAATGAATTCGGTATTGATCAGATTAGGTAT
TTTCTGATGCGTGAAGTAACTTTTGGTGCAGATGGCAATTTTGCCCGTAGTAATTTAATTACCCGTATTAATAGCGAGTT
GTCAAACAAAATAGGTAATTTATTACAGCGTAGTACAGCTTTTGTTTATAAAAATAATGAAGGTAAAGTGCCGCTTCTTA
CGCAAAGCGTTATAGATAAAATATATGAGCTGCCGATTTTAAAAACCGCAAGTAAATTTGCCGAGCAGAATATTTTATTG
ATGGACAAAACAGAGATTAATAAAATTCTTGACAATATTATTAATCTAGCTGAAGAAGCAAATATCTATATTGATAGTGA
AGCACCTTGGAATTTGAAAAAAACAGATCCGGATAAAATGCTAGAAGTGTTATACACTTTACTAGAAGTTTTACGCTATA
TTGCTATAATGCTTCAGCCTTTTATACCGAGTTCAGCGAATAAAATGCTAAATCAGTTAGGGATAAGTAAAGAAGAACGC
CTATTTAAGCATTTAATACGTGATTATGCTTTAAGGGCAGGTAGCAATATTTTAGAACCTACCATAATATTTCCGAGATT
TGAGGGGGATGTTGTATGA

Upstream 100 bases:

>100_bases
GCTTTAAAGCATTAAGTAATAGATTTCCTGAGAGAATAAAAACAATTAAAGCATCAGATTTAAGTCCACCTGAAGTACAT
GAGTTAATAAAAAAGCATTT

Downstream 100 bases:

>100_bases
ATCTTGTTATGTCCTTTCCAATTTCTTCTATATCATTCCCACGAGAGCTGGAATCTATTTTTTTAAATAGACTATATACT
AGCTTTTATATTCATTTTTT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MNNTYYITTPIYYVNDVPHIGHAYTSVASDVIARFMRLRGLDVIFLTGTDEHGQKVEKAAINKNIDPQKFTDQTSESFRH
LMAVMHISNDDFIRTTENRHKEAVAVFWQKLLDNGTIYEGFYEGWYAVRDEAFFDESELTSDGLAPTGAPVEWVKEPSYF
FNLSQWQDKLLEFYEANPDFIRPISRRNEVISFVKSGLKDLSVSRTTFNWGIKVPNNEKHVIYVWLDALANYISALGYPD
EQSSYGKFWPADLHVVGKDILRFHTVYWPAFLMAAEIPLPKTIMAHGWWTNEGQKISKSLGNTIDPIKLINEFGIDQIRY
FLMREVTFGADGNFARSNLITRINSELSNKIGNLLQRSTAFVYKNNEGKVPLLTQSVIDKIYELPILKTASKFAEQNILL
MDKTEINKILDNIINLAEEANIYIDSEAPWNLKKTDPDKMLEVLYTLLEVLRYIAIMLQPFIPSSANKMLNQLGISKEER
LFKHLIRDYALRAGSNILEPTIIFPRFEGDVV

Sequences:

>Translated_512_residues
MNNTYYITTPIYYVNDVPHIGHAYTSVASDVIARFMRLRGLDVIFLTGTDEHGQKVEKAAINKNIDPQKFTDQTSESFRH
LMAVMHISNDDFIRTTENRHKEAVAVFWQKLLDNGTIYEGFYEGWYAVRDEAFFDESELTSDGLAPTGAPVEWVKEPSYF
FNLSQWQDKLLEFYEANPDFIRPISRRNEVISFVKSGLKDLSVSRTTFNWGIKVPNNEKHVIYVWLDALANYISALGYPD
EQSSYGKFWPADLHVVGKDILRFHTVYWPAFLMAAEIPLPKTIMAHGWWTNEGQKISKSLGNTIDPIKLINEFGIDQIRY
FLMREVTFGADGNFARSNLITRINSELSNKIGNLLQRSTAFVYKNNEGKVPLLTQSVIDKIYELPILKTASKFAEQNILL
MDKTEINKILDNIINLAEEANIYIDSEAPWNLKKTDPDKMLEVLYTLLEVLRYIAIMLQPFIPSSANKMLNQLGISKEER
LFKHLIRDYALRAGSNILEPTIIFPRFEGDVV
>Mature_512_residues
MNNTYYITTPIYYVNDVPHIGHAYTSVASDVIARFMRLRGLDVIFLTGTDEHGQKVEKAAINKNIDPQKFTDQTSESFRH
LMAVMHISNDDFIRTTENRHKEAVAVFWQKLLDNGTIYEGFYEGWYAVRDEAFFDESELTSDGLAPTGAPVEWVKEPSYF
FNLSQWQDKLLEFYEANPDFIRPISRRNEVISFVKSGLKDLSVSRTTFNWGIKVPNNEKHVIYVWLDALANYISALGYPD
EQSSYGKFWPADLHVVGKDILRFHTVYWPAFLMAAEIPLPKTIMAHGWWTNEGQKISKSLGNTIDPIKLINEFGIDQIRY
FLMREVTFGADGNFARSNLITRINSELSNKIGNLLQRSTAFVYKNNEGKVPLLTQSVIDKIYELPILKTASKFAEQNILL
MDKTEINKILDNIINLAEEANIYIDSEAPWNLKKTDPDKMLEVLYTLLEVLRYIAIMLQPFIPSSANKMLNQLGISKEER
LFKHLIRDYALRAGSNILEPTIIFPRFEGDVV

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=508, Percent_Identity=38.7795275590551, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI14043022, Length=562, Percent_Identity=26.3345195729537, Blast_Score=143, Evalue=4e-34,
Organism=Escherichia coli, GI1788432, Length=498, Percent_Identity=25.1004016064257, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI212645096, Length=494, Percent_Identity=38.0566801619433, Blast_Score=335, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI71992781, Length=385, Percent_Identity=36.3636363636364, Blast_Score=238, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI17540794, Length=554, Percent_Identity=24.9097472924188, Blast_Score=138, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6321610, Length=523, Percent_Identity=33.8432122370937, Blast_Score=301, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6321704, Length=550, Percent_Identity=24.9090909090909, Blast_Score=127, Evalue=3e-30,
Organism=Drosophila melanogaster, GI21358483, Length=517, Percent_Identity=37.330754352031, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655311, Length=553, Percent_Identity=26.9439421338156, Blast_Score=155, Evalue=7e-38,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 58873; Mature: 58873

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNTYYITTPIYYVNDVPHIGHAYTSVASDVIARFMRLRGLDVIFLTGTDEHGQKVEKAA
CCCCEEEEECEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
INKNIDPQKFTDQTSESFRHLMAVMHISNDDFIRTTENRHKEAVAVFWQKLLDNGTIYEG
HCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHCCCCEEHH
FYEGWYAVRDEAFFDESELTSDGLAPTGAPVEWVKEPSYFFNLSQWQDKLLEFYEANPDF
HHHCHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHCCCHHEEEHHHHHHHHHHHHCCCCHH
IRPISRRNEVISFVKSGLKDLSVSRTTFNWGIKVPNNEKHVIYVWLDALANYISALGYPD
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECEECCCCCCEEEEEEHHHHHHHHHHCCCCC
EQSSYGKFWPADLHVVGKDILRFHTVYWPAFLMAAEIPLPKTIMAHGWWTNEGQKISKSL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHHH
GNTIDPIKLINEFGIDQIRYFLMREVTFGADGNFARSNLITRINSELSNKIGNLLQRSTA
CCCCCHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCE
FVYKNNEGKVPLLTQSVIDKIYELPILKTASKFAEQNILLMDKTEINKILDNIINLAEEA
EEEECCCCCCEEHHHHHHHHHHHCCHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHC
NIYIDSEAPWNLKKTDPDKMLEVLYTLLEVLRYIAIMLQPFIPSSANKMLNQLGISKEER
CEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHH
LFKHLIRDYALRAGSNILEPTIIFPRFEGDVV
HHHHHHHHHHHHCCCCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MNNTYYITTPIYYVNDVPHIGHAYTSVASDVIARFMRLRGLDVIFLTGTDEHGQKVEKAA
CCCCEEEEECEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
INKNIDPQKFTDQTSESFRHLMAVMHISNDDFIRTTENRHKEAVAVFWQKLLDNGTIYEG
HCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHCCCCEEHH
FYEGWYAVRDEAFFDESELTSDGLAPTGAPVEWVKEPSYFFNLSQWQDKLLEFYEANPDF
HHHCHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHCCCHHEEEHHHHHHHHHHHHCCCCHH
IRPISRRNEVISFVKSGLKDLSVSRTTFNWGIKVPNNEKHVIYVWLDALANYISALGYPD
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECEECCCCCCEEEEEEHHHHHHHHHHCCCCC
EQSSYGKFWPADLHVVGKDILRFHTVYWPAFLMAAEIPLPKTIMAHGWWTNEGQKISKSL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHHH
GNTIDPIKLINEFGIDQIRYFLMREVTFGADGNFARSNLITRINSELSNKIGNLLQRSTA
CCCCCHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCE
FVYKNNEGKVPLLTQSVIDKIYELPILKTASKFAEQNILLMDKTEINKILDNIINLAEEA
EEEECCCCCCEEHHHHHHHHHHHCCHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHC
NIYIDSEAPWNLKKTDPDKMLEVLYTLLEVLRYIAIMLQPFIPSSANKMLNQLGISKEER
CEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHH
LFKHLIRDYALRAGSNILEPTIIFPRFEGDVV
HHHHHHHHHHHHCCCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA