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The map label for this gene is ksgA

Identifier: 157826080

GI number: 157826080

Start: 927592

End: 928413

Strand: Reverse

Name: ksgA

Synonym: A1C_05200

Alternate gene names: 157826080

Gene position: 928413-927592 (Counterclockwise)

Preceding gene: 157826081

Following gene: 157826078

Centisome position: 75.42

GC content: 33.82

Gene sequence:

>822_bases
ATGCTCCCTTCAATTGCAAAACATGTAGCATCTCATCAAATTAATCCTCTTAAAAAGCACGGACAAAATTTTATTTTTGA
TAGTAGTTTATGTGATAAAATTGTACGTGCGAGTCACCTTGCAGAAAATAGCAGGGTGCTAGAAATAGGACCAGGCACCG
GAGGGTTAACTAGGTCAATATTGCAGAAAAATCCTGAATCCCTAACTGTTATAGAAACAGATGAGAGATTCATCCCGCTC
CTTAATGAGATTAAAGAATATTATCCTAATCTCAATATTATTAAACAAGATGCTCTTAAAATTAATTTAACTGATTTAGA
CTATGATAAGGTAACTATAATTTCTAATTTACCGTATCATATAGGTACTGAGTTAGTGATTAGATGGCTGAAAGAAGCAC
GGCTAATTACTGATATGACGCTAATGTTACAAAAGGAAGTAGTAGAACGTATTTGTGCTATGCCGTCAACTAAAGCATAT
GGCAGGTTATCGGTAATATGTCAGTTAATTGCTAAAGTGGAAAAATGCTTTGACGTATCACCGACTGCTTTCTATCCACC
TCCTAAAGTATATTCTGCAATAGTTAAACTAATACCTTTAGCAAATCCACCGTCTATTGCTCTAATAAATAGAGTTGAGA
AAATAACAACATTTGCTTTTGCAGGACGGCGGAAAATGATCAAATCATCACTGAAAAATCTTGTACCTAATATACATGAA
GTATTAACTCAGTTGAAAATCAATGATAATTATCGCGCTGAAAATCTTGCTCCTCAAGATTATTTGAGAATTGCTATGAG
TCTCTCAGTAAATAAACTTTAA

Upstream 100 bases:

>100_bases
AGTCTTTATTAATAAGATTTTTTTTTGATGCTGTGGTCAAGCAACGGCATGACACTGGAAAAATAACAAAATTCCATTTC
TACTAAAGCAACATTCCCTC

Downstream 100 bases:

>100_bases
TTAGTAATTCAAGAGTTTATTAGATATTATTAAGTTTGCATTATTCAGCTTTATTTAACTTTGATGCTTCCGCTTCTTGC
TGTAATTTATACTTTGCTGC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MLPSIAKHVASHQINPLKKHGQNFIFDSSLCDKIVRASHLAENSRVLEIGPGTGGLTRSILQKNPESLTVIETDERFIPL
LNEIKEYYPNLNIIKQDALKINLTDLDYDKVTIISNLPYHIGTELVIRWLKEARLITDMTLMLQKEVVERICAMPSTKAY
GRLSVICQLIAKVEKCFDVSPTAFYPPPKVYSAIVKLIPLANPPSIALINRVEKITTFAFAGRRKMIKSSLKNLVPNIHE
VLTQLKINDNYRAENLAPQDYLRIAMSLSVNKL

Sequences:

>Translated_273_residues
MLPSIAKHVASHQINPLKKHGQNFIFDSSLCDKIVRASHLAENSRVLEIGPGTGGLTRSILQKNPESLTVIETDERFIPL
LNEIKEYYPNLNIIKQDALKINLTDLDYDKVTIISNLPYHIGTELVIRWLKEARLITDMTLMLQKEVVERICAMPSTKAY
GRLSVICQLIAKVEKCFDVSPTAFYPPPKVYSAIVKLIPLANPPSIALINRVEKITTFAFAGRRKMIKSSLKNLVPNIHE
VLTQLKINDNYRAENLAPQDYLRIAMSLSVNKL
>Mature_273_residues
MLPSIAKHVASHQINPLKKHGQNFIFDSSLCDKIVRASHLAENSRVLEIGPGTGGLTRSILQKNPESLTVIETDERFIPL
LNEIKEYYPNLNIIKQDALKINLTDLDYDKVTIISNLPYHIGTELVIRWLKEARLITDMTLMLQKEVVERICAMPSTKAY
GRLSVICQLIAKVEKCFDVSPTAFYPPPKVYSAIVKLIPLANPPSIALINRVEKITTFAFAGRRKMIKSSLKNLVPNIHE
VLTQLKINDNYRAENLAPQDYLRIAMSLSVNKL

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=260, Percent_Identity=35, Blast_Score=141, Evalue=7e-34,
Organism=Homo sapiens, GI7657198, Length=235, Percent_Identity=30.2127659574468, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI1786236, Length=258, Percent_Identity=40.3100775193798, Blast_Score=167, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI25141369, Length=260, Percent_Identity=28.8461538461538, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25146882, Length=236, Percent_Identity=33.4745762711864, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324989, Length=216, Percent_Identity=30.0925925925926, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357273, Length=286, Percent_Identity=34.965034965035, Blast_Score=147, Evalue=8e-36,
Organism=Drosophila melanogaster, GI21358017, Length=256, Percent_Identity=30.46875, Blast_Score=104, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_RICAH (A8GPG7)

Other databases:

- EMBL:   CP000847
- RefSeq:   YP_001493800.1
- ProteinModelPortal:   A8GPG7
- SMR:   A8GPG7
- STRING:   A8GPG7
- GeneID:   5645394
- GenomeReviews:   CP000847_GR
- KEGG:   rak:A1C_05200
- NMPDR:   fig|293614.3.peg.979
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- ProtClustDB:   PRK00274
- BioCyc:   RAKA293614:A1C_05200-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30815; Mature: 30815

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 23-23 BINDING 25-25 BINDING 50-50 BINDING 72-72 BINDING 97-97 BINDING 116-116

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPSIAKHVASHQINPLKKHGQNFIFDSSLCDKIVRASHLAENSRVLEIGPGTGGLTRSI
CCCHHHHHHHHCCCCHHHHCCCCCEECHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHH
LQKNPESLTVIETDERFIPLLNEIKEYYPNLNIIKQDALKINLTDLDYDKVTIISNLPYH
HHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEECCCEEEEEEECCCCCEEEEECCCHH
IGTELVIRWLKEARLITDMTLMLQKEVVERICAMPSTKAYGRLSVICQLIAKVEKCFDVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
PTAFYPPPKVYSAIVKLIPLANPPSIALINRVEKITTFAFAGRRKMIKSSLKNLVPNIHE
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
VLTQLKINDNYRAENLAPQDYLRIAMSLSVNKL
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLPSIAKHVASHQINPLKKHGQNFIFDSSLCDKIVRASHLAENSRVLEIGPGTGGLTRSI
CCCHHHHHHHHCCCCHHHHCCCCCEECHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHH
LQKNPESLTVIETDERFIPLLNEIKEYYPNLNIIKQDALKINLTDLDYDKVTIISNLPYH
HHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEECCCEEEEEEECCCCCEEEEECCCHH
IGTELVIRWLKEARLITDMTLMLQKEVVERICAMPSTKAYGRLSVICQLIAKVEKCFDVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
PTAFYPPPKVYSAIVKLIPLANPPSIALINRVEKITTFAFAGRRKMIKSSLKNLVPNIHE
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
VLTQLKINDNYRAENLAPQDYLRIAMSLSVNKL
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA