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The map label for this gene is rlmB [H]

Identifier: 157826066

GI number: 157826066

Start: 915772

End: 916506

Strand: Direct

Name: rlmB [H]

Synonym: A1C_05120

Alternate gene names: 157826066

Gene position: 915772-916506 (Clockwise)

Preceding gene: 157826036

Following gene: 157826067

Centisome position: 74.39

GC content: 29.39

Gene sequence:

>735_bases
ATGGTTTTCAATAAGATGCAAAATAAAAAACTTGATTCTAAAAATTGTTATTATATGTATGGGAAACATCCTGTATTTTC
TGCTCTAAATAATCCAAAACGTCAAATTGAAAATATTTTATGCACTAGGGAAATTTTGGATACAAACAAAAAATTAATAG
GTACTAGATCTTATGAAATTGTTAATAACGATATTTTATCTAAATTACTTGGAAACCAAACACATCAAGGAATAGTTGCA
AAAGTAAGACCAATTTTTTCTTACAATTTAGAGAACTTAGATATAAAAAACCAAAAATGTAAAATTGCGATTCTTGATCA
AATAACAGACACACAAAATATTGGCGCCATTATTCGTAGTGCCGCTGCTTTTGATATCAATGCTATAATATTACCTAAAG
ATAATTCTCCGAATGAAAGCGGAGGCATTGCTAAAGCGGCTTGTGGTACTTTAGAATTAATATCTATAATTAAAGTGACT
AACTTACGCTCATGTATGAATTATCTTAAAAAACACGGCTTTTGGATTATAGGTCTAACCGGTTCTGCACATGATTACTT
TAGCGATAAATTAATTTCCGACAAAATGGCACTAGTATTTGGTTCAGAAGATAAAGGCATACGAAGATTAATCGAAGAAA
CATGTGATTATTTAGCTAAAATCCCTATTTCTAAGAGAGTTGAAAGTCTTAATGTATCAAATGCCGCTTCTATAATTTTT
CATTCATTACATTAA

Upstream 100 bases:

>100_bases
CTTCTTGCCGAATTATCAAACAAACAAATGGCAGCTTGCACATTTATTTAGTTGTAACTTTCTATAATAAATAGTAATTT
CTGTCAAGAACAAATTACTG

Downstream 100 bases:

>100_bases
TAATATTTTTAAATCAAATATTGTTTTTATTTACTGCGATATAAGTATCTAGTCTACTAAAATTAAAGTCATAAATTTTT
ATTAATGTGATAGAAAAGTC

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MVFNKMQNKKLDSKNCYYMYGKHPVFSALNNPKRQIENILCTREILDTNKKLIGTRSYEIVNNDILSKLLGNQTHQGIVA
KVRPIFSYNLENLDIKNQKCKIAILDQITDTQNIGAIIRSAAAFDINAIILPKDNSPNESGGIAKAACGTLELISIIKVT
NLRSCMNYLKKHGFWIIGLTGSAHDYFSDKLISDKMALVFGSEDKGIRRLIEETCDYLAKIPISKRVESLNVSNAASIIF
HSLH

Sequences:

>Translated_244_residues
MVFNKMQNKKLDSKNCYYMYGKHPVFSALNNPKRQIENILCTREILDTNKKLIGTRSYEIVNNDILSKLLGNQTHQGIVA
KVRPIFSYNLENLDIKNQKCKIAILDQITDTQNIGAIIRSAAAFDINAIILPKDNSPNESGGIAKAACGTLELISIIKVT
NLRSCMNYLKKHGFWIIGLTGSAHDYFSDKLISDKMALVFGSEDKGIRRLIEETCDYLAKIPISKRVESLNVSNAASIIF
HSLH
>Mature_244_residues
MVFNKMQNKKLDSKNCYYMYGKHPVFSALNNPKRQIENILCTREILDTNKKLIGTRSYEIVNNDILSKLLGNQTHQGIVA
KVRPIFSYNLENLDIKNQKCKIAILDQITDTQNIGAIIRSAAAFDINAIILPKDNSPNESGGIAKAACGTLELISIIKVT
NLRSCMNYLKKHGFWIIGLTGSAHDYFSDKLISDKMALVFGSEDKGIRRLIEETCDYLAKIPISKRVESLNVSNAASIIF
HSLH

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=183, Percent_Identity=38.2513661202186, Blast_Score=132, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17555908, Length=252, Percent_Identity=25.7936507936508, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6324775, Length=302, Percent_Identity=24.8344370860927, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27349; Mature: 27349

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFNKMQNKKLDSKNCYYMYGKHPVFSALNNPKRQIENILCTREILDTNKKLIGTRSYEI
CCCCCCCCCCCCCCCEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHCCCHHCCCCCHHH
VNNDILSKLLGNQTHQGIVAKVRPIFSYNLENLDIKNQKCKIAILDQITDTQNIGAIIRS
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHH
AAAFDINAIILPKDNSPNESGGIAKAACGTLELISIIKVTNLRSCMNYLKKHGFWIIGLT
HHHEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEC
GSAHDYFSDKLISDKMALVFGSEDKGIRRLIEETCDYLAKIPISKRVESLNVSNAASIIF
CCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHE
HSLH
ECCC
>Mature Secondary Structure
MVFNKMQNKKLDSKNCYYMYGKHPVFSALNNPKRQIENILCTREILDTNKKLIGTRSYEI
CCCCCCCCCCCCCCCEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHCCCHHCCCCCHHH
VNNDILSKLLGNQTHQGIVAKVRPIFSYNLENLDIKNQKCKIAILDQITDTQNIGAIIRS
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHH
AAAFDINAIILPKDNSPNESGGIAKAACGTLELISIIKVTNLRSCMNYLKKHGFWIIGLT
HHHEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEC
GSAHDYFSDKLISDKMALVFGSEDKGIRRLIEETCDYLAKIPISKRVESLNVSNAASIIF
CCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHE
HSLH
ECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]