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The map label for this gene is rpoA

Identifier: 157826039

GI number: 157826039

Start: 900693

End: 901715

Strand: Reverse

Name: rpoA

Synonym: A1C_04985

Alternate gene names: 157826039

Gene position: 901715-900693 (Counterclockwise)

Preceding gene: 157826040

Following gene: 157826038

Centisome position: 73.25

GC content: 31.77

Gene sequence:

>1023_bases
ATGTTATCGTTAAGTAAAAATTGGAATACTTTAATAAAGCCGAATAGAGTTGCTTATGAAAATTTTCCGGAAATCAATAA
TAAAGCTAAAATTGTAGTTGAACCTTTAGAAAGAGGTTTTGGTTTAACACTCGGTAATGCTATGAGAAGAGTGTTACTAT
CTTCTTTACAAGGAGCGGCTATCACTTCTATAAAGATTCCTGCTATAGAACATGAATTTTCTTCTATACCCGGTGTAAAA
GAAGATGTATCTGAAGTGATTTTAAACATTAAAGGTGTTGAATTAAAAATGCATGTTGCAGAAAAACGTATAATGAAATT
AAAAGCAACGGGACCTTGTGTTGTAACAGCCGGTATGATTGAAACAGGGCATGATGTAGAAATACTGAATCCTGATCATA
TAATTTGTAATCTAGCTAAGAACAAGCAACTTGAAATGGCATTAACTTGTAAGGTTGGTAAAGGATATGTACTTAGCACA
AATAGCTATGAGGATGATTTACCTATTGGTGAAATAGCTATAGATGCTTTATTTAACCCGGTCAAGAGTGTTACTTATAA
GGTAGAAAATACTAGGGTAGGTCAGGTTACCGACTATGATAAATTAATCATGTTTGTAGAAACTAACGGTGGTTTATTAC
CTGAAATGGCTGTGGGTTTAGCTGCACGTATCTTACAAGAGCAATTACAATTATTTATTTCTTTTGAAGAACATGAAGAA
GATAAGCAAGTTAAAACTGATACTTTACCATTTTCACCTTATTTACTTAAGAGAGTTGATGAATTGGAATTATCGGTGAG
ATCGGCAAATTGTTTGAAAAATGATAATATAATATATATAGGGGATCTAGTAAAAAGAACGGAATCTGATATGCTTAGAA
CTCCAAATTTCGGTAGAAAATCTTTAAATGAAATTAAAGAAATATTGGCGAAATTTAATTTGAGATTTGGTATGGATGTA
CCGGATTGGCCACCGGAAAATATTCAAGAATTATCTAAACGTTATGAAGATTCTTATAATTAA

Upstream 100 bases:

>100_bases
AAATTTTGTTGTTACGTCAATTTTAGATGTATCGTCAATTGCTCATAATGGAGTAAGACCGCCGAAAAGAAGAAGAGTAT
AAGAGAATCGTAGGTATTGA

Downstream 100 bases:

>100_bases
GGACTAATGAAAATGCGACATAAAATTAATGGTAGAAAGTTAAACGTAACAAGTAGTCATAGGCGAGCAATGCTCGCTAA
TATGGCTGTGGCACTTGTAA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MLSLSKNWNTLIKPNRVAYENFPEINNKAKIVVEPLERGFGLTLGNAMRRVLLSSLQGAAITSIKIPAIEHEFSSIPGVK
EDVSEVILNIKGVELKMHVAEKRIMKLKATGPCVVTAGMIETGHDVEILNPDHIICNLAKNKQLEMALTCKVGKGYVLST
NSYEDDLPIGEIAIDALFNPVKSVTYKVENTRVGQVTDYDKLIMFVETNGGLLPEMAVGLAARILQEQLQLFISFEEHEE
DKQVKTDTLPFSPYLLKRVDELELSVRSANCLKNDNIIYIGDLVKRTESDMLRTPNFGRKSLNEIKEILAKFNLRFGMDV
PDWPPENIQELSKRYEDSYN

Sequences:

>Translated_340_residues
MLSLSKNWNTLIKPNRVAYENFPEINNKAKIVVEPLERGFGLTLGNAMRRVLLSSLQGAAITSIKIPAIEHEFSSIPGVK
EDVSEVILNIKGVELKMHVAEKRIMKLKATGPCVVTAGMIETGHDVEILNPDHIICNLAKNKQLEMALTCKVGKGYVLST
NSYEDDLPIGEIAIDALFNPVKSVTYKVENTRVGQVTDYDKLIMFVETNGGLLPEMAVGLAARILQEQLQLFISFEEHEE
DKQVKTDTLPFSPYLLKRVDELELSVRSANCLKNDNIIYIGDLVKRTESDMLRTPNFGRKSLNEIKEILAKFNLRFGMDV
PDWPPENIQELSKRYEDSYN
>Mature_340_residues
MLSLSKNWNTLIKPNRVAYENFPEINNKAKIVVEPLERGFGLTLGNAMRRVLLSSLQGAAITSIKIPAIEHEFSSIPGVK
EDVSEVILNIKGVELKMHVAEKRIMKLKATGPCVVTAGMIETGHDVEILNPDHIICNLAKNKQLEMALTCKVGKGYVLST
NSYEDDLPIGEIAIDALFNPVKSVTYKVENTRVGQVTDYDKLIMFVETNGGLLPEMAVGLAARILQEQLQLFISFEEHEE
DKQVKTDTLPFSPYLLKRVDELELSVRSANCLKNDNIIYIGDLVKRTESDMLRTPNFGRKSLNEIKEILAKFNLRFGMDV
PDWPPENIQELSKRYEDSYN

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=324, Percent_Identity=45.679012345679, Blast_Score=286, Evalue=1e-78,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_RICAH (A8GPC6)

Other databases:

- EMBL:   CP000847
- RefSeq:   YP_001493759.1
- ProteinModelPortal:   A8GPC6
- STRING:   A8GPC6
- GeneID:   5645453
- GenomeReviews:   CP000847_GR
- KEGG:   rak:A1C_04985
- NMPDR:   fig|293614.3.peg.937
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   RAKA293614:A1C_04985-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 38267; Mature: 38267

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSLSKNWNTLIKPNRVAYENFPEINNKAKIVVEPLERGFGLTLGNAMRRVLLSSLQGAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCEEHHHHHHHHHHHHCCCCE
ITSIKIPAIEHEFSSIPGVKEDVSEVILNIKGVELKMHVAEKRIMKLKATGPCVVTAGMI
EEEEECCCCHHHHHCCCCCHHHHHHHHHHCCCEEEEEEHHHHHHHEEECCCCEEEEECCE
ETGHDVEILNPDHIICNLAKNKQLEMALTCKVGKGYVLSTNSYEDDLPIGEIAIDALFNP
ECCCCEEEECCCEEEEEECCCCCEEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHH
VKSVTYKVENTRVGQVTDYDKLIMFVETNGGLLPEMAVGLAARILQEQLQLFISFEEHEE
HHHHEEEECCCCCCCEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEECHHHCCC
DKQVKTDTLPFSPYLLKRVDELELSVRSANCLKNDNIIYIGDLVKRTESDMLRTPNFGRK
CHHHCCCCCCCCHHHHHHHHHHHHEEHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCHH
SLNEIKEILAKFNLRFGMDVPDWPPENIQELSKRYEDSYN
HHHHHHHHHHHHCCEECCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLSLSKNWNTLIKPNRVAYENFPEINNKAKIVVEPLERGFGLTLGNAMRRVLLSSLQGAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCEEHHHHHHHHHHHHCCCCE
ITSIKIPAIEHEFSSIPGVKEDVSEVILNIKGVELKMHVAEKRIMKLKATGPCVVTAGMI
EEEEECCCCHHHHHCCCCCHHHHHHHHHHCCCEEEEEEHHHHHHHEEECCCCEEEEECCE
ETGHDVEILNPDHIICNLAKNKQLEMALTCKVGKGYVLSTNSYEDDLPIGEIAIDALFNP
ECCCCEEEECCCEEEEEECCCCCEEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHH
VKSVTYKVENTRVGQVTDYDKLIMFVETNGGLLPEMAVGLAARILQEQLQLFISFEEHEE
HHHHEEEECCCCCCCEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEECHHHCCC
DKQVKTDTLPFSPYLLKRVDELELSVRSANCLKNDNIIYIGDLVKRTESDMLRTPNFGRK
CHHHCCCCCCCCHHHHHHHHHHHHEEHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCHH
SLNEIKEILAKFNLRFGMDVPDWPPENIQELSKRYEDSYN
HHHHHHHHHHHHCCEECCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA