Click here to switch to the map view.

The map label for this gene is grpE [H]

Identifier: 157826033

GI number: 157826033

Start: 895325

End: 895861

Strand: Direct

Name: grpE [H]

Synonym: A1C_04955

Alternate gene names: 157826033

Gene position: 895325-895861 (Clockwise)

Preceding gene: 157826026

Following gene: 157826034

Centisome position: 72.73

GC content: 32.96

Gene sequence:

>537_bases
ATGATAGATGATAATATAGAAAATAATGAACAAACCATAAATGATATTGCTGAAGAGATAGTTGAAAAGGCAAATCCGGA
AATAACAGAGTTAAAAGCAGAAATTGAAGAGCTAAAAGACAGACTCATTAGAACTACTGCCGAAATAGACAATACAAGAA
AACGTTTAGAAAAAGCACGTGACGAAGCAAAAGATTATGCAATTGCTACATTTGCTAAGGAGCTACTAAATGTTAGTGAT
AATCTTTCAAGAGCATTAGCACATAAGCCGGCAAATTCTGATATAGAAGTCACAAATATTATTGCAGGCGTTCAGATGAC
TAAAGATGAACTGGATAAAATCTTTCATAGACATCATATTGAAGAAATAAAGCCTGAAATAGGATCAATGTTCGACTATA
ATTTACATAATGCAATTTCGCAGATAGAACATCCTGATCATGAACCGAATAGCATTATTACTTTAATGCAATCAGGATAT
AAAATTAGAGATAGGCTGCTTCGCCCTGCTACCGTACAAGTAGCTAAAAAATCTTAA

Upstream 100 bases:

>100_bases
TACTAGTTTAATTTTTCAAATTCAACGAGTATACCATTGAAAAAATTTTTATTATATATATATATATCTCAAAAAAGAAT
AGAAATATAAAGTAAATAAT

Downstream 100 bases:

>100_bases
TACTAAGATAAAACTTCAAAAATTGGCGTTGTCGTCCTACAAGAGCTTCGGTTCGAATTATATTAAGTATACGCTGCGAT
CCTCACCTTGTGAACTCCTA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MIDDNIENNEQTINDIAEEIVEKANPEITELKAEIEELKDRLIRTTAEIDNTRKRLEKARDEAKDYAIATFAKELLNVSD
NLSRALAHKPANSDIEVTNIIAGVQMTKDELDKIFHRHHIEEIKPEIGSMFDYNLHNAISQIEHPDHEPNSIITLMQSGY
KIRDRLLRPATVQVAKKS

Sequences:

>Translated_178_residues
MIDDNIENNEQTINDIAEEIVEKANPEITELKAEIEELKDRLIRTTAEIDNTRKRLEKARDEAKDYAIATFAKELLNVSD
NLSRALAHKPANSDIEVTNIIAGVQMTKDELDKIFHRHHIEEIKPEIGSMFDYNLHNAISQIEHPDHEPNSIITLMQSGY
KIRDRLLRPATVQVAKKS
>Mature_178_residues
MIDDNIENNEQTINDIAEEIVEKANPEITELKAEIEELKDRLIRTTAEIDNTRKRLEKARDEAKDYAIATFAKELLNVSD
NLSRALAHKPANSDIEVTNIIAGVQMTKDELDKIFHRHHIEEIKPEIGSMFDYNLHNAISQIEHPDHEPNSIITLMQSGY
KIRDRLLRPATVQVAKKS

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=185, Percent_Identity=29.7297297297297, Blast_Score=87, Evalue=9e-18,
Organism=Homo sapiens, GI40255109, Length=178, Percent_Identity=25.2808988764045, Blast_Score=73, Evalue=1e-13,
Organism=Escherichia coli, GI1788967, Length=139, Percent_Identity=33.8129496402878, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17552458, Length=159, Percent_Identity=36.4779874213836, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324806, Length=173, Percent_Identity=35.2601156069364, Blast_Score=107, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24653432, Length=175, Percent_Identity=36.5714285714286, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 20391; Mature: 20391

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDDNIENNEQTINDIAEEIVEKANPEITELKAEIEELKDRLIRTTAEIDNTRKRLEKAR
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEAKDYAIATFAKELLNVSDNLSRALAHKPANSDIEVTNIIAGVQMTKDELDKIFHRHHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EEIKPEIGSMFDYNLHNAISQIEHPDHEPNSIITLMQSGYKIRDRLLRPATVQVAKKS
HHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHCCCHHHHHCCCC
>Mature Secondary Structure
MIDDNIENNEQTINDIAEEIVEKANPEITELKAEIEELKDRLIRTTAEIDNTRKRLEKAR
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEAKDYAIATFAKELLNVSDNLSRALAHKPANSDIEVTNIIAGVQMTKDELDKIFHRHHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EEIKPEIGSMFDYNLHNAISQIEHPDHEPNSIITLMQSGYKIRDRLLRPATVQVAKKS
HHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA