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The map label for this gene is rph

Identifier: 157826032

GI number: 157826032

Start: 894478

End: 895197

Strand: Reverse

Name: rph

Synonym: A1C_04950

Alternate gene names: 157826032

Gene position: 895197-894478 (Counterclockwise)

Preceding gene: 157826037

Following gene: 157826031

Centisome position: 72.72

GC content: 39.86

Gene sequence:

>720_bases
ATGAGACAGTCAGGAAGAAAAAGCAACCAATTACGTCCTATTTCATTAGAATTATCACCGCTTATTAATACGGAAGGTTC
GTGCATTATTAAAATCGGTAATACGCATGTCATGTGTAGTGCTACTTGCGAAACTACCGTACCACCGTTTTTACGAGGGC
AAAATCAAGGTTGGGTTACCGCTGAGTACGGCATGCTCCCAGGCTCTACATCCCAGCGTATTAAAAGAGAAGCAGCACAA
GGGAAGCAAGGCGGAAGAACCCAAGAAATACAGCGTTTGATAGGAAGAAGCATGCGATGTGTAATGGACTTGAAGAAACT
GGGTGAGAGGCAGATTATTATAGATTGTGACGTTATTAATGCCGATGGTGGTACTAGAACGGCATCGATAACAGGAAGCT
ATGTAGCATTACACCTAGCTATTAGATCATTGATGAAAAAGAGAATTTTAAAGGTCAATCCCTTAATTAGTCAAATTGCA
GCTGTTTCTTGCGGTATATATAAAGGTGAGGCGATACTAGATTTGGATTATTTAGAAGATAGTGATGCTGAAGTGGATAG
TAATTTTGTATTTGCAGGTAACGGTAATTTAATCGAAGTACAAGGCACGGCAGAGCAAGAACCTTTTTCCGAAGAGCAGT
TTATAGAAATGTTAAAACTTGCTAAATGCGGTGCAGCAGAATTATTTAAACTACAAAATCAAGTGCTGCTTGGGACTTAG

Upstream 100 bases:

>100_bases
TTTGAGATATATATATATATAATAAAAATTTTTTCAATGGTATACTCGTTGAATTTGAAAAATTAAACTAGTATACTACT
TATGGAGTACAAATTGTATT

Downstream 100 bases:

>100_bases
AGGTGTTATTTTGTGGGGCGGTAAAATGCTCTTGATGCATTCTCTGCGAGGCATTGCAAGAGTGGATACTGCTCCGTCAT
TGCAAACAGCTGTATGCTGC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MRQSGRKSNQLRPISLELSPLINTEGSCIIKIGNTHVMCSATCETTVPPFLRGQNQGWVTAEYGMLPGSTSQRIKREAAQ
GKQGGRTQEIQRLIGRSMRCVMDLKKLGERQIIIDCDVINADGGTRTASITGSYVALHLAIRSLMKKRILKVNPLISQIA
AVSCGIYKGEAILDLDYLEDSDAEVDSNFVFAGNGNLIEVQGTAEQEPFSEEQFIEMLKLAKCGAAELFKLQNQVLLGT

Sequences:

>Translated_239_residues
MRQSGRKSNQLRPISLELSPLINTEGSCIIKIGNTHVMCSATCETTVPPFLRGQNQGWVTAEYGMLPGSTSQRIKREAAQ
GKQGGRTQEIQRLIGRSMRCVMDLKKLGERQIIIDCDVINADGGTRTASITGSYVALHLAIRSLMKKRILKVNPLISQIA
AVSCGIYKGEAILDLDYLEDSDAEVDSNFVFAGNGNLIEVQGTAEQEPFSEEQFIEMLKLAKCGAAELFKLQNQVLLGT
>Mature_239_residues
MRQSGRKSNQLRPISLELSPLINTEGSCIIKIGNTHVMCSATCETTVPPFLRGQNQGWVTAEYGMLPGSTSQRIKREAAQ
GKQGGRTQEIQRLIGRSMRCVMDLKKLGERQIIIDCDVINADGGTRTASITGSYVALHLAIRSLMKKRILKVNPLISQIA
AVSCGIYKGEAILDLDYLEDSDAEVDSNFVFAGNGNLIEVQGTAEQEPFSEEQFIEMLKLAKCGAAELFKLQNQVLLGT

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=57.345971563981, Blast_Score=250, Evalue=7e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_RICAH (A8GPB9)

Other databases:

- EMBL:   CP000847
- RefSeq:   YP_001493752.1
- ProteinModelPortal:   A8GPB9
- SMR:   A8GPB9
- STRING:   A8GPB9
- GeneID:   5645875
- GenomeReviews:   CP000847_GR
- KEGG:   rak:A1C_04950
- NMPDR:   fig|293614.3.peg.928
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- BioCyc:   RAKA293614:A1C_04950-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 26150; Mature: 26150

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQSGRKSNQLRPISLELSPLINTEGSCIIKIGNTHVMCSATCETTVPPFLRGQNQGWVT
CCCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCEEEEEEECCCCCCCHHHCCCCCCEEE
AEYGMLPGSTSQRIKREAAQGKQGGRTQEIQRLIGRSMRCVMDLKKLGERQIIIDCDVIN
EECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASITGSYVALHLAIRSLMKKRILKVNPLISQIAAVSCGIYKGEAILDLDYLED
CCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCEEEEEECCCC
SDAEVDSNFVFAGNGNLIEVQGTAEQEPFSEEQFIEMLKLAKCGAAELFKLQNQVLLGT
CCCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCEEECCC
>Mature Secondary Structure
MRQSGRKSNQLRPISLELSPLINTEGSCIIKIGNTHVMCSATCETTVPPFLRGQNQGWVT
CCCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCEEEEEEECCCCCCCHHHCCCCCCEEE
AEYGMLPGSTSQRIKREAAQGKQGGRTQEIQRLIGRSMRCVMDLKKLGERQIIIDCDVIN
EECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASITGSYVALHLAIRSLMKKRILKVNPLISQIAAVSCGIYKGEAILDLDYLED
CCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCEEEEEECCCC
SDAEVDSNFVFAGNGNLIEVQGTAEQEPFSEEQFIEMLKLAKCGAAELFKLQNQVLLGT
CCCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA