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The map label for this gene is mraW

Identifier: 157825941

GI number: 157825941

Start: 794295

End: 795215

Strand: Reverse

Name: mraW

Synonym: A1C_04405

Alternate gene names: 157825941

Gene position: 795215-794295 (Counterclockwise)

Preceding gene: 157825942

Following gene: 157825940

Centisome position: 64.6

GC content: 35.94

Gene sequence:

>921_bases
ATGTTACAGACCCACATACCTGTAATGCTAAGCGAAGTATTGAAGGTTTTAGATCCAAAAGGCGGTGAGTTTTATTTGGA
TTGTACATTTGGAGCAGGAGGATATAGCAAAGCAATATTAGAAAGCTGTGACTGTTATGTTGTAGCTTTGGATCGTGATC
CAAACGTGATTAAAAAAGCTGAAGAAATTAAACAAAATTACGTTGAGAGATTCGATTTTATAGCAACGAATTTTGCGGAT
AGTTTTGCAAAGCTTAAGGAAAAACAATTTGACGGGATAGTGCTGGATTTAGGTGTCTCATCAATGCAGTTAGATGTAGC
AGATAGAGGATTTTCGTTTTTGCATGATGGACCACTAGATATGCGTATGAGCGGGCAAGGGTTTAGTGCCGAGGAATTTG
TAAATACGTTAGAGGAAAAAGAAATCGCCGATATAATTTATAAGTATGGTAATGAGAGCTTTTCACGCAGAATAGCTAAG
AGCATCGTAGAATATAGAAAAACGGCAAGAATTGATAGTACGGGCAAGCTCGCTGAGATTGTCAGGAGCAGTATAGGATT
CAGAAAAGGCAAAATTGATCCTGCAACTAAAACCTTTCAAGCTATTAGGATTTATATAAACGATGAGCTTGGGGAGTTAG
AGCGGTTTTTAATGAATGTAAAAAATATATTAAAGAAAGACGGACGTTTAGTTGTGGTATCTTTTCACTCTTTAGAAGAC
ATGATAATCAAGAATTTCTTTAAAGAAAATTCGGAAAAGCCGGTAGCAAGGTCAAAATATGCTAAAGATGATATAATAAT
TGATCCAAATAAATGGCTTAGGATTATTACTAACAAAGCTCTAGCACCCTCAGATAAAGAGATAAGGCTTAATATTAGAG
CGAGATCAGCAAAGCTTAGAACCGCAAAAAAGATACTATAG

Upstream 100 bases:

>100_bases
TGATTTTTGAGATTTGGCAGCCGCAGAATTTTGAGAAACATTTAAAATCTGCTCAAAAAATTGCTCATGAACAGCGGTTC
ACTCTACGGAATGCGAATTG

Downstream 100 bases:

>100_bases
CCTATGTCATTGTTGCATGGCTTGATTGATGTCATTCCGGCGTCGGCGGGAATCAAGTACTTTGTCATACCGCGACTTGA
TCGCGGTATCTAATTTATTA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MLQTHIPVMLSEVLKVLDPKGGEFYLDCTFGAGGYSKAILESCDCYVVALDRDPNVIKKAEEIKQNYVERFDFIATNFAD
SFAKLKEKQFDGIVLDLGVSSMQLDVADRGFSFLHDGPLDMRMSGQGFSAEEFVNTLEEKEIADIIYKYGNESFSRRIAK
SIVEYRKTARIDSTGKLAEIVRSSIGFRKGKIDPATKTFQAIRIYINDELGELERFLMNVKNILKKDGRLVVVSFHSLED
MIIKNFFKENSEKPVARSKYAKDDIIIDPNKWLRIITNKALAPSDKEIRLNIRARSAKLRTAKKIL

Sequences:

>Translated_306_residues
MLQTHIPVMLSEVLKVLDPKGGEFYLDCTFGAGGYSKAILESCDCYVVALDRDPNVIKKAEEIKQNYVERFDFIATNFAD
SFAKLKEKQFDGIVLDLGVSSMQLDVADRGFSFLHDGPLDMRMSGQGFSAEEFVNTLEEKEIADIIYKYGNESFSRRIAK
SIVEYRKTARIDSTGKLAEIVRSSIGFRKGKIDPATKTFQAIRIYINDELGELERFLMNVKNILKKDGRLVVVSFHSLED
MIIKNFFKENSEKPVARSKYAKDDIIIDPNKWLRIITNKALAPSDKEIRLNIRARSAKLRTAKKIL
>Mature_306_residues
MLQTHIPVMLSEVLKVLDPKGGEFYLDCTFGAGGYSKAILESCDCYVVALDRDPNVIKKAEEIKQNYVERFDFIATNFAD
SFAKLKEKQFDGIVLDLGVSSMQLDVADRGFSFLHDGPLDMRMSGQGFSAEEFVNTLEEKEIADIIYKYGNESFSRRIAK
SIVEYRKTARIDSTGKLAEIVRSSIGFRKGKIDPATKTFQAIRIYINDELGELERFLMNVKNILKKDGRLVVVSFHSLED
MIIKNFFKENSEKPVARSKYAKDDIIIDPNKWLRIITNKALAPSDKEIRLNIRARSAKLRTAKKIL

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=341, Percent_Identity=39.2961876832845, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI165377202, Length=191, Percent_Identity=41.8848167539267, Blast_Score=151, Evalue=6e-37,
Organism=Escherichia coli, GI1786270, Length=309, Percent_Identity=41.1003236245955, Blast_Score=213, Evalue=1e-56,
Organism=Drosophila melanogaster, GI62472493, Length=328, Percent_Identity=37.1951219512195, Blast_Score=192, Evalue=3e-49,
Organism=Drosophila melanogaster, GI28571637, Length=328, Percent_Identity=37.1951219512195, Blast_Score=192, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_RICAH (A8GP28)

Other databases:

- EMBL:   CP000847
- RefSeq:   YP_001493661.1
- ProteinModelPortal:   A8GP28
- SMR:   A8GP28
- STRING:   A8GP28
- GeneID:   5644851
- GenomeReviews:   CP000847_GR
- KEGG:   rak:A1C_04405
- NMPDR:   fig|293614.3.peg.818
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   RAKA293614:A1C_04405-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34801; Mature: 34801

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: BINDING 51-51 BINDING 82-82 BINDING 96-96 BINDING 103-103

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQTHIPVMLSEVLKVLDPKGGEFYLDCTFGAGGYSKAILESCDCYVVALDRDPNVIKKA
CCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHCCCCEEEEEECCCCHHHHHH
EEIKQNYVERFDFIATNFADSFAKLKEKQFDGIVLDLGVSSMQLDVADRGFSFLHDGPLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEHHHCCCHHHCCCCCC
MRMSGQGFSAEEFVNTLEEKEIADIIYKYGNESFSRRIAKSIVEYRKTARIDSTGKLAEI
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VRSSIGFRKGKIDPATKTFQAIRIYINDELGELERFLMNVKNILKKDGRLVVVSFHSLED
HHHHCCCCCCCCCHHHHHEEHEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHH
MIIKNFFKENSEKPVARSKYAKDDIIIDPNKWLRIITNKALAPSDKEIRLNIRARSAKLR
HHHHHHHHCCCCCCCHHHHCCCCCEEECCCHHHHHHHCCCCCCCCCEEEEEEEECHHHHH
TAKKIL
HHHHCC
>Mature Secondary Structure
MLQTHIPVMLSEVLKVLDPKGGEFYLDCTFGAGGYSKAILESCDCYVVALDRDPNVIKKA
CCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHCCCCEEEEEECCCCHHHHHH
EEIKQNYVERFDFIATNFADSFAKLKEKQFDGIVLDLGVSSMQLDVADRGFSFLHDGPLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEHHHCCCHHHCCCCCC
MRMSGQGFSAEEFVNTLEEKEIADIIYKYGNESFSRRIAKSIVEYRKTARIDSTGKLAEI
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VRSSIGFRKGKIDPATKTFQAIRIYINDELGELERFLMNVKNILKKDGRLVVVSFHSLED
HHHHCCCCCCCCCHHHHHEEHEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHH
MIIKNFFKENSEKPVARSKYAKDDIIIDPNKWLRIITNKALAPSDKEIRLNIRARSAKLR
HHHHHHHHCCCCCCCHHHHCCCCCEEECCCHHHHHHHCCCCCCCCCEEEEEEEECHHHHH
TAKKIL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA