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The map label for this gene is ispB [H]

Identifier: 157825829

GI number: 157825829

Start: 685755

End: 686738

Strand: Reverse

Name: ispB [H]

Synonym: A1C_03830

Alternate gene names: 157825829

Gene position: 686738-685755 (Counterclockwise)

Preceding gene: 157825830

Following gene: 157825806

Centisome position: 55.78

GC content: 31.81

Gene sequence:

>984_bases
ATGAATATTATAGTAAAAATACAGCAAGATTTAAAAGATGAAGTAACTAAGTTAAACGATTTAATCATTAGCTGTTTAAA
AAGTGATGAAGAGTTAATAGAAAAGGTCGGTAAATATTTACTAGAGGCAGGCGGTAAAAGAATTCGTCCGCTTTTAACTA
TAATAACCGCTAAAATGTTTGATTATAAAGGCGATAATCATATAAAGCTCGCAAGTGCCGTTGAATTCATTCACACCGCT
ACATTGCTTCATGATGATGTGGTAGACGACAGCACTTTAAGAAGATTCAAACCTACGGCTAACGTTATTTGGGGAAGTAA
AACAAGTATTTTAGTCGGTGATTTCCTATTTAGTCAGTCTTTTAAGTTAATGGTCGCTTCTGGCTCTATTAAAGCTATGA
ATGTTCTAGCTAAAGCTTCAGCAATTATTTCCGTAGGGGAGGTAATACAGCTAGTTAAGCTAAATGAGCGACGTATTATA
ACTATAGAAGAGTATTATCAAATAGTTAAGTCTAAAACTGCTGAGTTATTTGGTGCTTCTTGTGAAGTTGGAGCTATTAT
AGCAGAGCAGGTAGACCATATTTCTAAAGATATACGATACTTTGGGACATTACTCGGAACGATATTTCAGGTTATAGATG
ATCTACTTGATTATTTGGGTAATGATCAACAGGTAGGCAAAAATATCGGCGATGATTTCTTAGAAGGAAAAGTTACGTTA
CCGCTGATTTTTTTATATAATAAGCTAGAGCAAGATAAGCGTCTTTGGCTTGAAAATATAATTAAATGCGATAAGCGTAC
TAATGATGAGTTTATGCAAATACGTGACTTAATGGTAAAGCATGAAATCTACAATGAAACAATTAATTATTTGAGTAACT
TAGAACATGAAATAACTAAATTATTAAATAAAATTCCGGTTCAGAATATTTATAAAGATTATTTGTTTTCGATTATTAGA
TTTATTTTAGACCGTTCTTATTAA

Upstream 100 bases:

>100_bases
TCACCCCGTGACTTGATCACGGGTTCTATTAGTACTTTCTGGATACCACGGTTCAAGCCGCGGTATGACGCTAACAGGGC
TCTAGTAATAAAACAACACC

Downstream 100 bases:

>100_bases
TCGTGTAATGACAACATTTGCATTCGTAGCTCAGCTGGATAGAGCATTGCCCTCCGAAGGCAAAGGTCGTTGGTTCAAAT
CCAATCGAATGCACCATTTC

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MNIIVKIQQDLKDEVTKLNDLIISCLKSDEELIEKVGKYLLEAGGKRIRPLLTIITAKMFDYKGDNHIKLASAVEFIHTA
TLLHDDVVDDSTLRRFKPTANVIWGSKTSILVGDFLFSQSFKLMVASGSIKAMNVLAKASAIISVGEVIQLVKLNERRII
TIEEYYQIVKSKTAELFGASCEVGAIIAEQVDHISKDIRYFGTLLGTIFQVIDDLLDYLGNDQQVGKNIGDDFLEGKVTL
PLIFLYNKLEQDKRLWLENIIKCDKRTNDEFMQIRDLMVKHEIYNETINYLSNLEHEITKLLNKIPVQNIYKDYLFSIIR
FILDRSY

Sequences:

>Translated_327_residues
MNIIVKIQQDLKDEVTKLNDLIISCLKSDEELIEKVGKYLLEAGGKRIRPLLTIITAKMFDYKGDNHIKLASAVEFIHTA
TLLHDDVVDDSTLRRFKPTANVIWGSKTSILVGDFLFSQSFKLMVASGSIKAMNVLAKASAIISVGEVIQLVKLNERRII
TIEEYYQIVKSKTAELFGASCEVGAIIAEQVDHISKDIRYFGTLLGTIFQVIDDLLDYLGNDQQVGKNIGDDFLEGKVTL
PLIFLYNKLEQDKRLWLENIIKCDKRTNDEFMQIRDLMVKHEIYNETINYLSNLEHEITKLLNKIPVQNIYKDYLFSIIR
FILDRSY
>Mature_327_residues
MNIIVKIQQDLKDEVTKLNDLIISCLKSDEELIEKVGKYLLEAGGKRIRPLLTIITAKMFDYKGDNHIKLASAVEFIHTA
TLLHDDVVDDSTLRRFKPTANVIWGSKTSILVGDFLFSQSFKLMVASGSIKAMNVLAKASAIISVGEVIQLVKLNERRII
TIEEYYQIVKSKTAELFGASCEVGAIIAEQVDHISKDIRYFGTLLGTIFQVIDDLLDYLGNDQQVGKNIGDDFLEGKVTL
PLIFLYNKLEQDKRLWLENIIKCDKRTNDEFMQIRDLMVKHEIYNETINYLSNLEHEITKLLNKIPVQNIYKDYLFSIIR
FILDRSY

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=235, Percent_Identity=31.4893617021277, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI4758430, Length=206, Percent_Identity=25.2427184466019, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI83700220, Length=206, Percent_Identity=25.2427184466019, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789578, Length=238, Percent_Identity=44.9579831932773, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1786623, Length=224, Percent_Identity=31.25, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17505681, Length=350, Percent_Identity=23.4285714285714, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6319475, Length=221, Percent_Identity=28.5067873303167, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6325188, Length=239, Percent_Identity=25.5230125523013, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24651612, Length=254, Percent_Identity=28.740157480315, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI281365769, Length=255, Percent_Identity=22.3529411764706, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24660002, Length=255, Percent_Identity=22.3529411764706, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 37446; Mature: 37446

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIVKIQQDLKDEVTKLNDLIISCLKSDEELIEKVGKYLLEAGGKRIRPLLTIITAKMF
CCEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
DYKGDNHIKLASAVEFIHTATLLHDDVVDDSTLRRFKPTANVIWGSKTSILVGDFLFSQS
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEECCCCHHHHHHHHHCCC
FKLMVASGSIKAMNVLAKASAIISVGEVIQLVKLNERRIITIEEYYQIVKSKTAELFGAS
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCC
CEVGAIIAEQVDHISKDIRYFGTLLGTIFQVIDDLLDYLGNDQQVGKNIGDDFLEGKVTL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHCCCHHH
PLIFLYNKLEQDKRLWLENIIKCDKRTNDEFMQIRDLMVKHEIYNETINYLSNLEHEITK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLNKIPVQNIYKDYLFSIIRFILDRSY
HHHHCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNIIVKIQQDLKDEVTKLNDLIISCLKSDEELIEKVGKYLLEAGGKRIRPLLTIITAKMF
CCEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
DYKGDNHIKLASAVEFIHTATLLHDDVVDDSTLRRFKPTANVIWGSKTSILVGDFLFSQS
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEECCCCHHHHHHHHHCCC
FKLMVASGSIKAMNVLAKASAIISVGEVIQLVKLNERRIITIEEYYQIVKSKTAELFGAS
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCC
CEVGAIIAEQVDHISKDIRYFGTLLGTIFQVIDDLLDYLGNDQQVGKNIGDDFLEGKVTL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHCCCHHH
PLIFLYNKLEQDKRLWLENIIKCDKRTNDEFMQIRDLMVKHEIYNETINYLSNLEHEITK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLNKIPVQNIYKDYLFSIIRFILDRSY
HHHHCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]