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The map label for this gene is truB

Identifier: 157825783

GI number: 157825783

Start: 643912

End: 644793

Strand: Reverse

Name: truB

Synonym: A1C_03570

Alternate gene names: 157825783

Gene position: 644793-643912 (Counterclockwise)

Preceding gene: 157825786

Following gene: 157825782

Centisome position: 52.38

GC content: 34.92

Gene sequence:

>882_bases
ATGAGTAATTATTGGTTAAATATTTATAAACCAGGAGGTATAAGTTCTGCTAAACTGGTTAGCATGGTAAAACAAATACT
CGGTAAAAAAGTTAAGGTAGGACATGCCGGTACTTTAGATGTTGAAGCGGAAGGGATACTACCCATTGCCGTAGGTGAAG
CTACAAAGCTGATACAGCTGCTAATTGATTCTAGAAAAACCTATATTTTTACAATAAAATTTGGACTACAAACCGATAGT
GGCGATTATACCGGCACCGTAATAGCAACAAAAAATTATATCCCTTCTCAAGAAGAGGCTTATACCGTATGTTCTAAGTT
TATCGGTAAAGTAACACAAATACCGCCGGCTTTTTCAGCTCTTAAAGTTAACGGTGTCAGAGCTTATAAATTTGCTAGAG
AGGGGAAAGAAGTAGAATTAAAGCCACGAAATATAACGATTTATAATCTAAAATGTTTAAATTTTGATAAGAAAAATGCT
ATCGCTATATACTATACGGAATGCTCAAAAGGTACTTATATAAGGACTTTAGCAGAAGATTTGGCATTGTCCTTGCAAAG
TTTAGGATTTGTGATAGAATTACGCCGTACTCAGGTTGGAATATTTAAAGCAGAAAATGCTATCCGAATTAAATCGCCTG
ACGAAATTACGAAAAGTTTCCTAGAGGAAAAAAGCATAAAGATAGAAGCAATACTGGACGACATCCTGGTTCTTGATGCA
ACTGACAGCCAAGCACAGCAAATTAAATATGGACAGAAATGCATATTTGATTATGAAGACGACATTAGTCTTTTATGGGT
TCGCTATAAGGGTACTCTGCTTGCAATAGGTAGCTTAAACAAGAATTGCTTTAATTCTTTACGTGTGTTTAATGTATTAT
AA

Upstream 100 bases:

>100_bases
CTATTGGCTAGAGTTACATATTAAAGATTTTTTAAACTTGAGTCAAGGATATACTCATTTAATTTGAGGTATGAACCATT
TATTTTATAGAAGTATATTA

Downstream 100 bases:

>100_bases
CAATTTAAGGAGAAATTATTGATGTCGATTACTAAAGAACGTAAACAACAATTAATTAAAGAATATGCTATAACGGAAAA
TGATACCGGTTCAAGTGCGG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MSNYWLNIYKPGGISSAKLVSMVKQILGKKVKVGHAGTLDVEAEGILPIAVGEATKLIQLLIDSRKTYIFTIKFGLQTDS
GDYTGTVIATKNYIPSQEEAYTVCSKFIGKVTQIPPAFSALKVNGVRAYKFAREGKEVELKPRNITIYNLKCLNFDKKNA
IAIYYTECSKGTYIRTLAEDLALSLQSLGFVIELRRTQVGIFKAENAIRIKSPDEITKSFLEEKSIKIEAILDDILVLDA
TDSQAQQIKYGQKCIFDYEDDISLLWVRYKGTLLAIGSLNKNCFNSLRVFNVL

Sequences:

>Translated_293_residues
MSNYWLNIYKPGGISSAKLVSMVKQILGKKVKVGHAGTLDVEAEGILPIAVGEATKLIQLLIDSRKTYIFTIKFGLQTDS
GDYTGTVIATKNYIPSQEEAYTVCSKFIGKVTQIPPAFSALKVNGVRAYKFAREGKEVELKPRNITIYNLKCLNFDKKNA
IAIYYTECSKGTYIRTLAEDLALSLQSLGFVIELRRTQVGIFKAENAIRIKSPDEITKSFLEEKSIKIEAILDDILVLDA
TDSQAQQIKYGQKCIFDYEDDISLLWVRYKGTLLAIGSLNKNCFNSLRVFNVL
>Mature_292_residues
SNYWLNIYKPGGISSAKLVSMVKQILGKKVKVGHAGTLDVEAEGILPIAVGEATKLIQLLIDSRKTYIFTIKFGLQTDSG
DYTGTVIATKNYIPSQEEAYTVCSKFIGKVTQIPPAFSALKVNGVRAYKFAREGKEVELKPRNITIYNLKCLNFDKKNAI
AIYYTECSKGTYIRTLAEDLALSLQSLGFVIELRRTQVGIFKAENAIRIKSPDEITKSFLEEKSIKIEAILDDILVLDAT
DSQAQQIKYGQKCIFDYEDDISLLWVRYKGTLLAIGSLNKNCFNSLRVFNVL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=219, Percent_Identity=33.7899543378995, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI4503337, Length=273, Percent_Identity=25.6410256410256, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI215599015, Length=273, Percent_Identity=25.6410256410256, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI2367200, Length=243, Percent_Identity=36.2139917695473, Blast_Score=135, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17553978, Length=306, Percent_Identity=24.8366013071895, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324037, Length=130, Percent_Identity=40, Blast_Score=90, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323204, Length=201, Percent_Identity=27.363184079602, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281364189, Length=278, Percent_Identity=26.978417266187, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281364187, Length=278, Percent_Identity=26.978417266187, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281364185, Length=278, Percent_Identity=26.978417266187, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281364183, Length=278, Percent_Identity=26.978417266187, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI62471759, Length=278, Percent_Identity=26.978417266187, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17975520, Length=278, Percent_Identity=26.978417266187, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_RICAH (A8GNM0)

Other databases:

- EMBL:   CP000847
- RefSeq:   YP_001493503.1
- ProteinModelPortal:   A8GNM0
- SMR:   A8GNM0
- STRING:   A8GNM0
- GeneID:   5645134
- GenomeReviews:   CP000847_GR
- KEGG:   rak:A1C_03570
- NMPDR:   fig|293614.3.peg.667
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   ASTKAYD
- ProtClustDB:   PRK01528
- BioCyc:   RAKA293614:A1C_03570-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 32752; Mature: 32621

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: ACT_SITE 40-40

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNYWLNIYKPGGISSAKLVSMVKQILGKKVKVGHAGTLDVEAEGILPIAVGEATKLIQL
CCCEEEEEECCCCCCHHHHHHHHHHHHCCEEEECCCCEEEEECCCEEEEEECCHHHHHHH
LIDSRKTYIFTIKFGLQTDSGDYTGTVIATKNYIPSQEEAYTVCSKFIGKVTQIPPAFSA
HHCCCCEEEEEEEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCE
LKVNGVRAYKFAREGKEVELKPRNITIYNLKCLNFDKKNAIAIYYTECSKGTYIRTLAED
EEECCEEEEEHHCCCCEEEEECCEEEEEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHH
LALSLQSLGFVIELRRTQVGIFKAENAIRIKSPDEITKSFLEEKSIKIEAILDDILVLDA
HHHHHHHCCEEEEEECCEEEEEECCCEEEECCHHHHHHHHHHHCCEEEEEEEEEEEEEEC
TDSQAQQIKYGQKCIFDYEDDISLLWVRYKGTLLAIGSLNKNCFNSLRVFNVL
CCCHHHHHHCCCEEEEECCCCEEEEEEEECCEEEEEECCCHHHHHHEEEEECC
>Mature Secondary Structure 
SNYWLNIYKPGGISSAKLVSMVKQILGKKVKVGHAGTLDVEAEGILPIAVGEATKLIQL
CCEEEEEECCCCCCHHHHHHHHHHHHCCEEEECCCCEEEEECCCEEEEEECCHHHHHHH
LIDSRKTYIFTIKFGLQTDSGDYTGTVIATKNYIPSQEEAYTVCSKFIGKVTQIPPAFSA
HHCCCCEEEEEEEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCE
LKVNGVRAYKFAREGKEVELKPRNITIYNLKCLNFDKKNAIAIYYTECSKGTYIRTLAED
EEECCEEEEEHHCCCCEEEEECCEEEEEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHH
LALSLQSLGFVIELRRTQVGIFKAENAIRIKSPDEITKSFLEEKSIKIEAILDDILVLDA
HHHHHHHCCEEEEEECCEEEEEECCCEEEECCHHHHHHHHHHHCCEEEEEEEEEEEEEEC
TDSQAQQIKYGQKCIFDYEDDISLLWVRYKGTLLAIGSLNKNCFNSLRVFNVL
CCCHHHHHHCCCEEEEECCCCEEEEEEEECCEEEEEECCCHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA