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The map label for this gene is pnp [H]

Identifier: 157825781

GI number: 157825781

Start: 641211

End: 643454

Strand: Reverse

Name: pnp [H]

Synonym: A1C_03560

Alternate gene names: 157825781

Gene position: 643454-641211 (Counterclockwise)

Preceding gene: 157825782

Following gene: 157825780

Centisome position: 52.27

GC content: 35.38

Gene sequence:

>2244_bases
ATGTTTAACGAAATAACTAAGAGTGTTACATGGAACGGGAAAGTTCTTGAGCTTAGTACAGGTAAAATAGCACGTCAGGC
TGATGGGGCAGTTACGGTGAAAATGGGTAATTCCGTTTTGTTATGTGCGGCTGTAGTAGCTAACAAAGCAAAAGAGGGTA
TAGGTTTTTTTCCTTTAACAATTAATTATAGAGAAATGGCATATGCTGCCGGTAAGATACCAGGGGGATTTTTTAAACGT
GAGGGAAAAGCATCAGATAGAGAAGTTTTAGTATCTCGTTTAATAGATAGACCGATTAGACCGTTATTTCATCCGGCTTT
TGTTAATGAAACTCATGTAACTTGTACTGTTCTGTCATATGATCCTGAGACTCCAGTAGATATACTTGCAATTATCGGTG
CATCGGCTGCACTTAGTCTATCCCCTGCTCCTTATCTAGAAATAGTTGCTGCAAGTAAAGTAGGTTTAATCGACGGTGAA
TTTGTTTTAAACCCGACACTTGAATTATTAAAAACAAGTCAGCTGGATTTAGTAGTTGCAGGAACAGCAGACTCGGTAAT
GATGGTTGAATCGGAAGCTAATTTACTCTCTGAAGAACAAATGTTAGAAGCCGTAAAGTTTGGGTTTGAAAGCTTCCAGC
CGATAATAAAGATCATTAAAGAACTAGCAAAAGAAGCTAAAAAACCAAAGTTTCAAATGCAAGATTTATATCCTGCCTCA
TTAAAGAAAGAAATTGAGAAGTTATTTGTAAAAGAAATTAAACAAGCTTTTGCGATTAAATCTAAACAGGAACGTAGTAC
TAATTTAGATTTAATACATGAAAAAGTGCTTACACATTTTGTAAGCGATATAGAAAATAAAAAATATAGTAATTATCAAA
TCGAATCGGCTTTAAAATCTATTGAATCAGATATATTGCGTAACGAAATTTTAGAAAAAAATAGACGTATCGATGGAAGA
AGTACTACGGATATAAGACAAATTGCTTGTGAAATAGGTTTGCTACCTTCCGCACACGGTTCAGCTTTATTTACTAGAGG
GGAAACGCAGAGCTTAGTTAGCACTACATTCGGTACTAGTTTAGATGAGCAGCTAGTTGACAGTTTAGATGGTGAGTATA
AAGAGCGTTTTATGCTTGATTATATCTTCCCACCCTATTCGGTAAATGAAGCAATGCCGATGAAAGCACCTAGTCGTCGT
GAAGTCGGACACGGTAAACTCGCATGGCGTGCTATTAATCCAATATTACCTAATAAAGTACAATTTCCTTATGCTATTAG
AGTAGTTGCTGAAACTACGGAGTCTAACGGTTCTTCTTCAATGGCAACTGTTTGCAGTAGTTCTCTTGCTTTAATGTATG
CAGGCGTACCGATAAAAGCACCGGTTGCGGGTATTGCTATGGGACTTGTTAAAGAAGGCAAAAAATTTGCCGTATTATCG
GATATTCTCGGCGATGAAGATTATTTCGGTGATATGGACTTTAAGGTTGCAGGAACTAGCGAAGGGATTACTGCATTACA
AATGGATATCAAAATATCCGGAGTAGATTTTAAAATAATGAAAGCAGCTTTAGAGCAAGCACGACTCGGTCGTTTGCATA
TACTGGATCAAATGAATAAAATTATCAGTAAACCAAATAGCAAACTAGGTAAGAATGTTCCTTCTACTACTACTATAAAA
ATAGATAAGGATAAAATTAGAGATGTTATAGGACCGGGCGGTAAAGTAATAAAGGAAATTTGTGAGACTAGCGATGCTAA
AATAGATATAAGCGATGACGGTACGGTTTCTGTTTATGCTTCAGATAGAGATAAGCTAAAAGTCGCTTTAGATAAAATTA
AAGCTATTGCCGTTGAGCCTGAAATCGGTGAAATATTTAACGGTACGGTAATGAAAGTCTTAGATTCCGGTGCTTTTATT
AATTATTTAGGTACTAAAGATGGTTTTGTTCATATTAGTGAAATTGCAGAAGAAAGAATAGAAACGGTTGGCAGTGTTTT
AAAACAAGGCGATATAGTAAAAGTTAAGCTAATAGGCTTTGACAATAAAGGCAAAGCAAAACTAACTATTAAAAATGCCG
ATAAGGATAAATCTTCAAATAATCCAAAGCAAAAAAATAATGTTAATAATTCTAAGGAAAACTCAGAACCTGAGCGACGT
GATTCTAGTAAGAAACGAGCTTGGAATGAAGATAATAATTCTGACACTACTGAAGTAATTACAGAACGTAAGTATTTTAA
TTAA

Upstream 100 bases:

>100_bases
TTAAAAAGCTTTAAAGTAATGGATTCCCACTTTCGCGGAAACGACATAATAAAAATCCGGAATAAAAAAGAAAAATTAAA
TTATGCAATGAGATAAATAG

Downstream 100 bases:

>100_bases
ATGTCATTTGTTGTGTGGATCAATTTATGTCATGCCTGCGTAGGCGGGAATCCAGTCAAAACCTATAAAAAACTTGTTTT
TTAGATTTATTTCATCAAAT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 747; Mature: 747

Protein sequence:

>747_residues
MFNEITKSVTWNGKVLELSTGKIARQADGAVTVKMGNSVLLCAAVVANKAKEGIGFFPLTINYREMAYAAGKIPGGFFKR
EGKASDREVLVSRLIDRPIRPLFHPAFVNETHVTCTVLSYDPETPVDILAIIGASAALSLSPAPYLEIVAASKVGLIDGE
FVLNPTLELLKTSQLDLVVAGTADSVMMVESEANLLSEEQMLEAVKFGFESFQPIIKIIKELAKEAKKPKFQMQDLYPAS
LKKEIEKLFVKEIKQAFAIKSKQERSTNLDLIHEKVLTHFVSDIENKKYSNYQIESALKSIESDILRNEILEKNRRIDGR
STTDIRQIACEIGLLPSAHGSALFTRGETQSLVSTTFGTSLDEQLVDSLDGEYKERFMLDYIFPPYSVNEAMPMKAPSRR
EVGHGKLAWRAINPILPNKVQFPYAIRVVAETTESNGSSSMATVCSSSLALMYAGVPIKAPVAGIAMGLVKEGKKFAVLS
DILGDEDYFGDMDFKVAGTSEGITALQMDIKISGVDFKIMKAALEQARLGRLHILDQMNKIISKPNSKLGKNVPSTTTIK
IDKDKIRDVIGPGGKVIKEICETSDAKIDISDDGTVSVYASDRDKLKVALDKIKAIAVEPEIGEIFNGTVMKVLDSGAFI
NYLGTKDGFVHISEIAEERIETVGSVLKQGDIVKVKLIGFDNKGKAKLTIKNADKDKSSNNPKQKNNVNNSKENSEPERR
DSSKKRAWNEDNNSDTTEVITERKYFN

Sequences:

>Translated_747_residues
MFNEITKSVTWNGKVLELSTGKIARQADGAVTVKMGNSVLLCAAVVANKAKEGIGFFPLTINYREMAYAAGKIPGGFFKR
EGKASDREVLVSRLIDRPIRPLFHPAFVNETHVTCTVLSYDPETPVDILAIIGASAALSLSPAPYLEIVAASKVGLIDGE
FVLNPTLELLKTSQLDLVVAGTADSVMMVESEANLLSEEQMLEAVKFGFESFQPIIKIIKELAKEAKKPKFQMQDLYPAS
LKKEIEKLFVKEIKQAFAIKSKQERSTNLDLIHEKVLTHFVSDIENKKYSNYQIESALKSIESDILRNEILEKNRRIDGR
STTDIRQIACEIGLLPSAHGSALFTRGETQSLVSTTFGTSLDEQLVDSLDGEYKERFMLDYIFPPYSVNEAMPMKAPSRR
EVGHGKLAWRAINPILPNKVQFPYAIRVVAETTESNGSSSMATVCSSSLALMYAGVPIKAPVAGIAMGLVKEGKKFAVLS
DILGDEDYFGDMDFKVAGTSEGITALQMDIKISGVDFKIMKAALEQARLGRLHILDQMNKIISKPNSKLGKNVPSTTTIK
IDKDKIRDVIGPGGKVIKEICETSDAKIDISDDGTVSVYASDRDKLKVALDKIKAIAVEPEIGEIFNGTVMKVLDSGAFI
NYLGTKDGFVHISEIAEERIETVGSVLKQGDIVKVKLIGFDNKGKAKLTIKNADKDKSSNNPKQKNNVNNSKENSEPERR
DSSKKRAWNEDNNSDTTEVITERKYFN
>Mature_747_residues
MFNEITKSVTWNGKVLELSTGKIARQADGAVTVKMGNSVLLCAAVVANKAKEGIGFFPLTINYREMAYAAGKIPGGFFKR
EGKASDREVLVSRLIDRPIRPLFHPAFVNETHVTCTVLSYDPETPVDILAIIGASAALSLSPAPYLEIVAASKVGLIDGE
FVLNPTLELLKTSQLDLVVAGTADSVMMVESEANLLSEEQMLEAVKFGFESFQPIIKIIKELAKEAKKPKFQMQDLYPAS
LKKEIEKLFVKEIKQAFAIKSKQERSTNLDLIHEKVLTHFVSDIENKKYSNYQIESALKSIESDILRNEILEKNRRIDGR
STTDIRQIACEIGLLPSAHGSALFTRGETQSLVSTTFGTSLDEQLVDSLDGEYKERFMLDYIFPPYSVNEAMPMKAPSRR
EVGHGKLAWRAINPILPNKVQFPYAIRVVAETTESNGSSSMATVCSSSLALMYAGVPIKAPVAGIAMGLVKEGKKFAVLS
DILGDEDYFGDMDFKVAGTSEGITALQMDIKISGVDFKIMKAALEQARLGRLHILDQMNKIISKPNSKLGKNVPSTTTIK
IDKDKIRDVIGPGGKVIKEICETSDAKIDISDDGTVSVYASDRDKLKVALDKIKAIAVEPEIGEIFNGTVMKVLDSGAFI
NYLGTKDGFVHISEIAEERIETVGSVLKQGDIVKVKLIGFDNKGKAKLTIKNADKDKSSNNPKQKNNVNNSKENSEPERR
DSSKKRAWNEDNNSDTTEVITERKYFN

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=712, Percent_Identity=37.3595505617978, Blast_Score=432, Evalue=1e-121,
Organism=Escherichia coli, GI145693187, Length=694, Percent_Identity=49.7118155619597, Blast_Score=676, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=728, Percent_Identity=32.1428571428571, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI281362905, Length=699, Percent_Identity=34.7639484978541, Blast_Score=413, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24651641, Length=699, Percent_Identity=34.7639484978541, Blast_Score=413, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24651643, Length=699, Percent_Identity=34.7639484978541, Blast_Score=413, Evalue=1e-115,
Organism=Drosophila melanogaster, GI161079377, Length=688, Percent_Identity=33.8662790697674, Blast_Score=377, Evalue=1e-104,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 82183; Mature: 82183

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNEITKSVTWNGKVLELSTGKIARQADGAVTVKMGNSVLLCAAVVANKAKEGIGFFPLT
CCCHHHCCEECCCEEEEECCCCHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEE
INYREMAYAAGKIPGGFFKREGKASDREVLVSRLIDRPIRPLFHPAFVNETHVTCTVLSY
ECHHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHHCCCCHHHHCHHHCCCCEEEEEEEEC
DPETPVDILAIIGASAALSLSPAPYLEIVAASKVGLIDGEFVLNPTLELLKTSQLDLVVA
CCCCCHHHHHHHCCCCEEECCCCCCEEEEECCCCCEECCCEEECCHHHHHCCCCCCEEEE
GTADSVMMVESEANLLSEEQMLEAVKFGFESFQPIIKIIKELAKEAKKPKFQMQDLYPAS
CCCCCEEEEECCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHH
LKKEIEKLFVKEIKQAFAIKSKQERSTNLDLIHEKVLTHFVSDIENKKYSNYQIESALKS
HHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
IESDILRNEILEKNRRIDGRSTTDIRQIACEIGLLPSAHGSALFTRGETQSLVSTTFGTS
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHCCC
LDEQLVDSLDGEYKERFMLDYIFPPYSVNEAMPMKAPSRREVGHGKLAWRAINPILPNKV
HHHHHHHHCCCHHHHHEEHEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
QFPYAIRVVAETTESNGSSSMATVCSSSLALMYAGVPIKAPVAGIAMGLVKEGKKFAVLS
CCCEEEEEEEECCCCCCCCHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCCEEHHH
DILGDEDYFGDMDFKVAGTSEGITALQMDIKISGVDFKIMKAALEQARLGRLHILDQMNK
HHHCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHH
IISKPNSKLGKNVPSTTTIKIDKDKIRDVIGPGGKVIKEICETSDAKIDISDDGTVSVYA
HHCCCCHHHCCCCCCCEEEEECHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCEEEEEE
SDRDKLKVALDKIKAIAVEPEIGEIFNGTVMKVLDSGAFINYLGTKDGFVHISEIAEERI
CCCHHHHHHHHHHHHEEECCCHHHHHCCHHHHHHHCCCEEEECCCCCCCEEHHHHHHHHH
ETVGSVLKQGDIVKVKLIGFDNKGKAKLTIKNADKDKSSNNPKQKNNVNNSKENSEPERR
HHHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCCCCCCCCCHHCCCCCCCCCCCCHHH
DSSKKRAWNEDNNSDTTEVITERKYFN
CCHHHHCCCCCCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MFNEITKSVTWNGKVLELSTGKIARQADGAVTVKMGNSVLLCAAVVANKAKEGIGFFPLT
CCCHHHCCEECCCEEEEECCCCHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEE
INYREMAYAAGKIPGGFFKREGKASDREVLVSRLIDRPIRPLFHPAFVNETHVTCTVLSY
ECHHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHHCCCCHHHHCHHHCCCCEEEEEEEEC
DPETPVDILAIIGASAALSLSPAPYLEIVAASKVGLIDGEFVLNPTLELLKTSQLDLVVA
CCCCCHHHHHHHCCCCEEECCCCCCEEEEECCCCCEECCCEEECCHHHHHCCCCCCEEEE
GTADSVMMVESEANLLSEEQMLEAVKFGFESFQPIIKIIKELAKEAKKPKFQMQDLYPAS
CCCCCEEEEECCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHH
LKKEIEKLFVKEIKQAFAIKSKQERSTNLDLIHEKVLTHFVSDIENKKYSNYQIESALKS
HHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
IESDILRNEILEKNRRIDGRSTTDIRQIACEIGLLPSAHGSALFTRGETQSLVSTTFGTS
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHCCC
LDEQLVDSLDGEYKERFMLDYIFPPYSVNEAMPMKAPSRREVGHGKLAWRAINPILPNKV
HHHHHHHHCCCHHHHHEEHEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
QFPYAIRVVAETTESNGSSSMATVCSSSLALMYAGVPIKAPVAGIAMGLVKEGKKFAVLS
CCCEEEEEEEECCCCCCCCHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCCEEHHH
DILGDEDYFGDMDFKVAGTSEGITALQMDIKISGVDFKIMKAALEQARLGRLHILDQMNK
HHHCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHH
IISKPNSKLGKNVPSTTTIKIDKDKIRDVIGPGGKVIKEICETSDAKIDISDDGTVSVYA
HHCCCCHHHCCCCCCCEEEEECHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCEEEEEE
SDRDKLKVALDKIKAIAVEPEIGEIFNGTVMKVLDSGAFINYLGTKDGFVHISEIAEERI
CCCHHHHHHHHHHHHEEECCCHHHHHCCHHHHHHHCCCEEEECCCCCCCEEHHHHHHHHH
ETVGSVLKQGDIVKVKLIGFDNKGKAKLTIKNADKDKSSNNPKQKNNVNNSKENSEPERR
HHHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCCCCCCCCCHHCCCCCCCCCCCCHHH
DSSKKRAWNEDNNSDTTEVITERKYFN
CCHHHHCCCCCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]