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The map label for this gene is folD

Identifier: 157825763

GI number: 157825763

Start: 623031

End: 623918

Strand: Reverse

Name: folD

Synonym: A1C_03455

Alternate gene names: 157825763

Gene position: 623918-623031 (Counterclockwise)

Preceding gene: 157825764

Following gene: 157825759

Centisome position: 50.68

GC content: 31.98

Gene sequence:

>888_bases
GTGAATAATATAATTGACGGTAAAGCGTTAGCAAATGAGATACTAGAGGATTTAAAGCTAGAAATTCAAGAACTTAAAAG
TCAAACCAACGCTTCACCAAAGCTTGCTATAGTACTAGTTGGCGATAATCCTGCAAGTATTATTTATGTAAAAAATAAAA
TAAAAAATGCACATAAAATAGGAATCGATACTTTATTAGTAAATCTATCTACTACTATTCATACAAATGATTTAATAACA
AAAATCAATGAGTTAAACCATGATAACGAGATTTCGGGAATGATAGTTCAGCTTCCTTTGCCAAACTCCATAGATAAAAA
TAAAATTTTATCTGCTGTATCACCATATAAAGATGTTGATGGGTTTCATCCTTTAAATGTTGGTTACTTACATAGCGGTA
TTATAGATGTAAGTGAATTCAGTCAAAGTTTTATACCATGCACTGCCCTTGGTTGTCTTGCGGTGATAAAAAAATACGAA
CCTAATTTAAGTGGTAAACACGTTGTAGTTATTGGACGCTCAAATATAGTAGGAAAACCTTTAAGTGCTTTACTATTAAA
AGAAGATTGCAGTGTTACTATTTGCCATTCTAAAACACATAATTTAGGCTCTATCGCTTTAAAAGCGGATATTGTAGTTG
CTGCAATAGGTTCGCCTTTAAAATTAACTGCAGAATATTTTAATCCTGAATCGATAGTAATTGATGTAGGCATTAATAGA
ATTAGCGGCAACAAAATTATCGGTGATGTTGATTTTGAGCATGTTAAGTCTAAAGTAAAATATATTACTCCAGTCCCGGG
TGGCATCGGACCGATGACTATTGCCTTTTTACTTAAAAATACGGTTAAGGCGTTTAAGGATTCTTATAGTACAGTATGTC
ATTTATGA

Upstream 100 bases:

>100_bases
CAGAATTTTATATTATTATACGGTATCCATAAAAACAATAAAAATACCTTGAACACGCGATCAAGTCGCTGGATGATAAC
TGAAAAAGGATAAAAATATA

Downstream 100 bases:

>100_bases
CTTTAGCGACTTTGTTGCATGGTTTAAGAACAGTGTTGTCATTCATACGAAAGCGGGAATCCAAAAGTATTATAAGTACA
GCAGATTTTGGCAATTAAAG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MNNIIDGKALANEILEDLKLEIQELKSQTNASPKLAIVLVGDNPASIIYVKNKIKNAHKIGIDTLLVNLSTTIHTNDLIT
KINELNHDNEISGMIVQLPLPNSIDKNKILSAVSPYKDVDGFHPLNVGYLHSGIIDVSEFSQSFIPCTALGCLAVIKKYE
PNLSGKHVVVIGRSNIVGKPLSALLLKEDCSVTICHSKTHNLGSIALKADIVVAAIGSPLKLTAEYFNPESIVIDVGINR
ISGNKIIGDVDFEHVKSKVKYITPVPGGIGPMTIAFLLKNTVKAFKDSYSTVCHL

Sequences:

>Translated_295_residues
MNNIIDGKALANEILEDLKLEIQELKSQTNASPKLAIVLVGDNPASIIYVKNKIKNAHKIGIDTLLVNLSTTIHTNDLIT
KINELNHDNEISGMIVQLPLPNSIDKNKILSAVSPYKDVDGFHPLNVGYLHSGIIDVSEFSQSFIPCTALGCLAVIKKYE
PNLSGKHVVVIGRSNIVGKPLSALLLKEDCSVTICHSKTHNLGSIALKADIVVAAIGSPLKLTAEYFNPESIVIDVGINR
ISGNKIIGDVDFEHVKSKVKYITPVPGGIGPMTIAFLLKNTVKAFKDSYSTVCHL
>Mature_295_residues
MNNIIDGKALANEILEDLKLEIQELKSQTNASPKLAIVLVGDNPASIIYVKNKIKNAHKIGIDTLLVNLSTTIHTNDLIT
KINELNHDNEISGMIVQLPLPNSIDKNKILSAVSPYKDVDGFHPLNVGYLHSGIIDVSEFSQSFIPCTALGCLAVIKKYE
PNLSGKHVVVIGRSNIVGKPLSALLLKEDCSVTICHSKTHNLGSIALKADIVVAAIGSPLKLTAEYFNPESIVIDVGINR
ISGNKIIGDVDFEHVKSKVKYITPVPGGIGPMTIAFLLKNTVKAFKDSYSTVCHL

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=293, Percent_Identity=40.6143344709898, Blast_Score=224, Evalue=5e-59,
Organism=Homo sapiens, GI94721354, Length=298, Percent_Identity=42.6174496644295, Blast_Score=211, Evalue=8e-55,
Organism=Homo sapiens, GI222418558, Length=298, Percent_Identity=41.2751677852349, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI36796743, Length=237, Percent_Identity=25.3164556962025, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1786741, Length=281, Percent_Identity=42.3487544483986, Blast_Score=229, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17568735, Length=293, Percent_Identity=41.9795221843003, Blast_Score=215, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6319558, Length=294, Percent_Identity=41.4965986394558, Blast_Score=229, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6321643, Length=299, Percent_Identity=39.4648829431438, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6322933, Length=322, Percent_Identity=25.4658385093168, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI62472483, Length=290, Percent_Identity=43.1034482758621, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI45551871, Length=290, Percent_Identity=43.1034482758621, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24645718, Length=290, Percent_Identity=43.1034482758621, Blast_Score=222, Evalue=2e-58,
Organism=Drosophila melanogaster, GI17137370, Length=290, Percent_Identity=43.1034482758621, Blast_Score=222, Evalue=2e-58,
Organism=Drosophila melanogaster, GI17136818, Length=304, Percent_Identity=38.1578947368421, Blast_Score=201, Evalue=5e-52,
Organism=Drosophila melanogaster, GI17136816, Length=304, Percent_Identity=38.1578947368421, Blast_Score=201, Evalue=6e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_RICAH (A8GNK0)

Other databases:

- EMBL:   CP000847
- RefSeq:   YP_001493483.1
- ProteinModelPortal:   A8GNK0
- SMR:   A8GNK0
- STRING:   A8GNK0
- GeneID:   5645470
- GenomeReviews:   CP000847_GR
- KEGG:   rak:A1C_03455
- NMPDR:   fig|293614.3.peg.645
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   TYVRNKQ
- ProtClustDB:   PRK14171
- BioCyc:   RAKA293614:A1C_03455-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31876; Mature: 31876

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNIIDGKALANEILEDLKLEIQELKSQTNASPKLAIVLVGDNPASIIYVKNKIKNAHKI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEHHCCCCHHC
GIDTLLVNLSTTIHTNDLITKINELNHDNEISGMIVQLPLPNSIDKNKILSAVSPYKDVD
CCEEEEEEEEEEEECHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCHHCC
GFHPLNVGYLHSGIIDVSEFSQSFIPCTALGCLAVIKKYEPNLSGKHVVVIGRSNIVGKP
CCCCCCCCHHHCCCEEHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCC
LSALLLKEDCSVTICHSKTHNLGSIALKADIVVAAIGSPLKLTAEYFNPESIVIDVGINR
HHHHHHHCCCCEEEEECCCCCCCEEEEEEEEEEEECCCCEEEEEEECCCCEEEEEECCEE
ISGNKIIGDVDFEHVKSKVKYITPVPGGIGPMTIAFLLKNTVKAFKDSYSTVCHL
ECCCEEEECCCHHHHHHHCEEEECCCCCCHHHHHHHHHHHHHHHHHHCHHHHCCC
>Mature Secondary Structure
MNNIIDGKALANEILEDLKLEIQELKSQTNASPKLAIVLVGDNPASIIYVKNKIKNAHKI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEHHCCCCHHC
GIDTLLVNLSTTIHTNDLITKINELNHDNEISGMIVQLPLPNSIDKNKILSAVSPYKDVD
CCEEEEEEEEEEEECHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCHHCC
GFHPLNVGYLHSGIIDVSEFSQSFIPCTALGCLAVIKKYEPNLSGKHVVVIGRSNIVGKP
CCCCCCCCHHHCCCEEHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCC
LSALLLKEDCSVTICHSKTHNLGSIALKADIVVAAIGSPLKLTAEYFNPESIVIDVGINR
HHHHHHHCCCCEEEEECCCCCCCEEEEEEEEEEEECCCCEEEEEEECCCCEEEEEECCEE
ISGNKIIGDVDFEHVKSKVKYITPVPGGIGPMTIAFLLKNTVKAFKDSYSTVCHL
ECCCEEEECCCHHHHHHHCEEEECCCCCCHHHHHHHHHHHHHHHHHHCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA