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The map label for this gene is psdht [H]

Identifier: 157825756

GI number: 157825756

Start: 615269

End: 616270

Strand: Reverse

Name: psdht [H]

Synonym: A1C_03410

Alternate gene names: 157825756

Gene position: 616270-615269 (Counterclockwise)

Preceding gene: 157825758

Following gene: 157825738

Centisome position: 50.06

GC content: 34.73

Gene sequence:

>1002_bases
ATGACTTTATTACTACAATCTTCTCAAAATGTAGCTACTGCACATACTAGAATAAAACAATATCTACATTTAACTCCAAT
CGTTCACTCTGAAAGCTTAAATGAGATGCTCGGTCATGAGATTTTTTTTAAGGTTGAATCATTACAAAAAACCGGTGCAT
TCAAAGTTAGAGGAGTATTAAATCATTTATTAGAATTAAAAGAGCAAGGCAAATTACCTGATAAAATAGTCTGTTATAGT
ACGGGTAACCATGGGGTTGGTCTTGCTTATGCAAGTAAATTATTCGGTCTTAAAACAAGGATTTATTTACCGTTAAATAC
TTCAAAAGTAAAACAGCAAGCAGCTCTTTATTACGGCGGTGAAGTTATATATACATATACTCGACAAGAAGCAGAAGAAA
AAGCAAAAGAAGATGAAAAGCAAGGATTTTACTATATACATCCTTCCGATAGTGATTCTACAATAGCAGGAGCAGGCACT
TTATGCTATGAAGCATTACAGCAGCTAGGCTTTAGTCCTGATGCTATTTTTGCCTCTTGCGGCGGCGGCGGTTTAATTTC
CGGCTCATACCTTGCCAAGGAATTAATATCACCGATAAGCTTGTTAATAGGTTCAGAACCGCTTACTGCTAATGATGCTT
ATTTATCGGTTAAAAATAATAAGATATATAAATTTGATAAGGCACCGACTACCATCGCAGACGGTCTTAAAACCTTAAGT
GTATCAGCCCGTACATTTGAATATCTGAAAAAACTTGATCATTTTTATTTAGCAGAAGAATATGAAATATATTACTGGAC
GGCGTGGCTTACTAATTTACTTAAAGTTATATGCGAGCCGTCAAGTAGTATAAATATGGTGTCAGTGGTGAATTGGTTAA
AAATACAATCCAAACCTCAAAAATTACTGGTACTAATCTCAGGCGGTAATATAGACCCTATTCTTTATAACGAACTATGG
AAAGAGGAGTATTTAACCGTTCCACCAAGTATTACTAACTAA

Upstream 100 bases:

>100_bases
GGACTTTTTATTCATCAATTAAAACCTTTAAAAATTTCTAATTATTAACTATATAATATAGTATAAATGCGATTGCAAGC
CATCTTTATTAATTATATTT

Downstream 100 bases:

>100_bases
AGATCAATATCGTCATTGCGAGAAGAAACTATAAGTTTCTACGAAGCAATCTCAGGATATTTGACAAGATTGTTATGCAA
CTTACGGCTGCTCGCAAGGC

Product: threonine dehydratase

Products: 2-oxobutanoate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MTLLLQSSQNVATAHTRIKQYLHLTPIVHSESLNEMLGHEIFFKVESLQKTGAFKVRGVLNHLLELKEQGKLPDKIVCYS
TGNHGVGLAYASKLFGLKTRIYLPLNTSKVKQQAALYYGGEVIYTYTRQEAEEKAKEDEKQGFYYIHPSDSDSTIAGAGT
LCYEALQQLGFSPDAIFASCGGGGLISGSYLAKELISPISLLIGSEPLTANDAYLSVKNNKIYKFDKAPTTIADGLKTLS
VSARTFEYLKKLDHFYLAEEYEIYYWTAWLTNLLKVICEPSSSINMVSVVNWLKIQSKPQKLLVLISGGNIDPILYNELW
KEEYLTVPPSITN

Sequences:

>Translated_333_residues
MTLLLQSSQNVATAHTRIKQYLHLTPIVHSESLNEMLGHEIFFKVESLQKTGAFKVRGVLNHLLELKEQGKLPDKIVCYS
TGNHGVGLAYASKLFGLKTRIYLPLNTSKVKQQAALYYGGEVIYTYTRQEAEEKAKEDEKQGFYYIHPSDSDSTIAGAGT
LCYEALQQLGFSPDAIFASCGGGGLISGSYLAKELISPISLLIGSEPLTANDAYLSVKNNKIYKFDKAPTTIADGLKTLS
VSARTFEYLKKLDHFYLAEEYEIYYWTAWLTNLLKVICEPSSSINMVSVVNWLKIQSKPQKLLVLISGGNIDPILYNELW
KEEYLTVPPSITN
>Mature_332_residues
TLLLQSSQNVATAHTRIKQYLHLTPIVHSESLNEMLGHEIFFKVESLQKTGAFKVRGVLNHLLELKEQGKLPDKIVCYST
GNHGVGLAYASKLFGLKTRIYLPLNTSKVKQQAALYYGGEVIYTYTRQEAEEKAKEDEKQGFYYIHPSDSDSTIAGAGTL
CYEALQQLGFSPDAIFASCGGGGLISGSYLAKELISPISLLIGSEPLTANDAYLSVKNNKIYKFDKAPTTIADGLKTLSV
SARTFEYLKKLDHFYLAEEYEIYYWTAWLTNLLKVICEPSSSINMVSVVNWLKIQSKPQKLLVLISGGNIDPILYNELWK
EEYLTVPPSITN

Specific function: Acts On Both Serine And Threonine, And Properly Considered As A Hydroxy Amino Acid Deaminase. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=311, Percent_Identity=32.475884244373, Blast_Score=175, Evalue=5e-44,
Organism=Escherichia coli, GI1789505, Length=311, Percent_Identity=28.6173633440514, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790207, Length=295, Percent_Identity=28.135593220339, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI2367138, Length=199, Percent_Identity=25.6281407035176, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17508781, Length=314, Percent_Identity=29.9363057324841, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71991565, Length=309, Percent_Identity=28.4789644012945, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17537387, Length=314, Percent_Identity=28.0254777070064, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6322631, Length=330, Percent_Identity=32.1212121212121, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320930, Length=302, Percent_Identity=27.8145695364238, Blast_Score=82, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6319788, Length=263, Percent_Identity=25.4752851711027, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21355833, Length=314, Percent_Identity=28.343949044586, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19

Molecular weight: Translated: 37157; Mature: 37026

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLLQSSQNVATAHTRIKQYLHLTPIVHSESLNEMLGHEIFFKVESLQKTGAFKVRGVL
CEEEEECCCCHHHHHHHHHHHHHHCCEECHHHHHHHHCHHHEEEEHHHHHCCCCHHHHHH
NHLLELKEQGKLPDKIVCYSTGNHGVGLAYASKLFGLKTRIYLPLNTSKVKQQAALYYGG
HHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCEEEEEEECCHHHHHHHHHEEECC
EVIYTYTRQEAEEKAKEDEKQGFYYIHPSDSDSTIAGAGTLCYEALQQLGFSPDAIFASC
EEEEEEEHHHHHHHHHHHHHCCEEEECCCCCCCEECCHHHHHHHHHHHCCCCCCCEEEEC
GGGGLISGSYLAKELISPISLLIGSEPLTANDAYLSVKNNKIYKFDKAPTTIADGLKTLS
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEECCCCCHHHCCHHHEE
VSARTFEYLKKLDHFYLAEEYEIYYWTAWLTNLLKVICEPSSSINMVSVVNWLKIQSKPQ
HHHHHHHHHHHHHHHEEEECEEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCC
KLLVLISGGNIDPILYNELWKEEYLTVPPSITN
EEEEEEECCCCCHHHHHHHHHHHCEECCCCCCC
>Mature Secondary Structure 
TLLLQSSQNVATAHTRIKQYLHLTPIVHSESLNEMLGHEIFFKVESLQKTGAFKVRGVL
EEEEECCCCHHHHHHHHHHHHHHCCEECHHHHHHHHCHHHEEEEHHHHHCCCCHHHHHH
NHLLELKEQGKLPDKIVCYSTGNHGVGLAYASKLFGLKTRIYLPLNTSKVKQQAALYYGG
HHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCEEEEEEECCHHHHHHHHHEEECC
EVIYTYTRQEAEEKAKEDEKQGFYYIHPSDSDSTIAGAGTLCYEALQQLGFSPDAIFASC
EEEEEEEHHHHHHHHHHHHHCCEEEECCCCCCCEECCHHHHHHHHHHHCCCCCCCEEEEC
GGGGLISGSYLAKELISPISLLIGSEPLTANDAYLSVKNNKIYKFDKAPTTIADGLKTLS
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEECCCCCHHHCCHHHEE
VSARTFEYLKKLDHFYLAEEYEIYYWTAWLTNLLKVICEPSSSINMVSVVNWLKIQSKPQ
HHHHHHHHHHHHHHHEEEECEEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCC
KLLVLISGGNIDPILYNELWKEEYLTVPPSITN
EEEEEEECCCCCHHHHHHHHHHHCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} 40 {L-Ser}} 5 {L-Ser}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = 2-oxobutanoate + NH3

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]