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The map label for this gene is lepA [H]

Identifier: 157825494

GI number: 157825494

Start: 370172

End: 371974

Strand: Direct

Name: lepA [H]

Synonym: A1C_01975

Alternate gene names: 157825494

Gene position: 370172-371974 (Clockwise)

Preceding gene: 157825492

Following gene: 157825495

Centisome position: 30.07

GC content: 38.55

Gene sequence:

>1803_bases
ATGAAGAATCAAAAATATATACGGAATTTCTCAATAATTGCCCATATCGATCATGGTAAATCTACGTTAGCTGATAGGTT
AATTGAGCATTGCGGTGGGCTTCAGGCTAGAGAAATGAGAACGCAAGTGCTAGATTCTATGGATATCGAAAAAGAGCGAG
GTATAACTATTAAAGCACAAACCGTGCGGCTTGTATATAAAGCTAAAGACGGTAATACCTATTATTTGAATCTAATGGAC
ACTCCTGGGCATGTCGATTTTGCTTATGAGGTCAGCAGGTCGCTTGCTGCTTGCGAAGGCTCATTGTTGGTAGTTGATAG
TACGCAAGGAGTAGAAGCCCAAACGCTTGCCAATGTTTATCAAGCAATTGAGCATGATCATGAAATTGTACCGGTGCTTA
ACAAGATTGATTTGCCAGCGTCAGAACCTGAGCAGGTTAAGCAGCAAATAGAGGATATAATCGGAATTGATGCAAGTGAA
GCTGTGCTAATCTCTGCCAAAAGCGGTATAGGAATTGATTTAGTTCTAGAAGCAATAGTCAATAAATTACATCCACCAAA
AGAAAGTAGCACAGATATCTTAAAAGCGTTACTTGTCGATAGTTGGTATGATCCTTATCTTGGGGTAGTTATTTTAGTAC
GTATTATTGATGGGACTTTACGTAAAAACATGCGGATTAAAATGATGGCTACCAATTCTGTTTATACCGTTGAGAATGTC
GGCTTTTTTACTCCGAAAAAACATATTTCGGATGTTTTATATGCAGGTGAAATCGGTTTTGTTACTGCTTCTATAAAGCA
AGTAGCTGATTGTAAAGTCGGGGATACTATCACTGATGAGAAGAAACCTTGTGAGCAAGCACTTCCCGGCTTTAAGCCGA
ACCTACCTGTAGTGTTTTGTGGGCTTTATCCGACAGATAGTTCAGAGTTTGAACATCTAAAGGATTCGCTAGCTAAATTA
CGTCTTAATGATGCTAGTTTTGAGTATGAAATGGAAAGCTCTTCAGCTCTTGGAGTAGGTTTTAGGTGCGGTTTTTTAGG
GCTATTACATTTAGAGATAATCCAAGAACGTTTAAGTAGGGAATTCGATTTAGATTTAATCACTACTGCTCCAAGCGTGG
TATATAAAATTCATATGAGGTATGGTGAGAGCTTGGAGATTCATAACCCCGCAGATTTACCTGATTTGCAAAAAATCGAA
TCGATGGAAGAGCCATGGATTAAGGCAACTATAATGGTGCCTGATGAGTTTTTAGGTGCAGTATTATCGCTTTGTACAGA
AAAAAGAGGTGTGCAACTTGATCATAGCTATATAGCGAATAGAGCTAAAATAGTTTATAAATTACCGCTAAATGAGATAG
TTTACGATTTTTACGATCGTTTAAAAAGCTGCTCTAAAGGTTATGCAAGTTTTGAATGGCAAATAGATGTTTATGAACCT
TCTGAGCTTGTTAAGCTTGGTATCCTGGTTAACGGCGAGGTAATTGATGCATTATCGACAATCGTACACCGCTCACGTGC
CGAGCAAAGGGGTAAGGTGTTATGCGTAAGGTTAAAGGATTTAATACCAAGACAGCAGATCGATATAGCCATTCAGGCAA
GTATCGGTAGCCGTATTATTGCTCGTGAGACTATTAAAGCACTACGTAAAGATGTTTTATCAAAATGTTACGGCGGTGAT
ATAAGCCGTAAACGTAAACTACTTGAGAAGCAAAAAGCAGGTAAAAAGAGAATGAGACAGTACGGTAATATAGAAATCCC
GCAATCTGCATTTATCGCCGCTTTAAAAATAGGTGATGAATAG

Upstream 100 bases:

>100_bases
TCACGATATCTTGTATCACAGTTTGTGTCCTGAGATTCCACGATCAAGTTGTGGGACGGTACCGCTCCAACAAGCAAACG
CAAATTATATCACAATAATT

Downstream 100 bases:

>100_bases
ATGGAAACTATATTTTTAGAAGTAATTTGTACGTCGCTCTGTTACTCGAATGCTCATATACATTTGAGTACGTTGCAGTT
CGAAGTGAAGTGTCGGTGTA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MKNQKYIRNFSIIAHIDHGKSTLADRLIEHCGGLQAREMRTQVLDSMDIEKERGITIKAQTVRLVYKAKDGNTYYLNLMD
TPGHVDFAYEVSRSLAACEGSLLVVDSTQGVEAQTLANVYQAIEHDHEIVPVLNKIDLPASEPEQVKQQIEDIIGIDASE
AVLISAKSGIGIDLVLEAIVNKLHPPKESSTDILKALLVDSWYDPYLGVVILVRIIDGTLRKNMRIKMMATNSVYTVENV
GFFTPKKHISDVLYAGEIGFVTASIKQVADCKVGDTITDEKKPCEQALPGFKPNLPVVFCGLYPTDSSEFEHLKDSLAKL
RLNDASFEYEMESSSALGVGFRCGFLGLLHLEIIQERLSREFDLDLITTAPSVVYKIHMRYGESLEIHNPADLPDLQKIE
SMEEPWIKATIMVPDEFLGAVLSLCTEKRGVQLDHSYIANRAKIVYKLPLNEIVYDFYDRLKSCSKGYASFEWQIDVYEP
SELVKLGILVNGEVIDALSTIVHRSRAEQRGKVLCVRLKDLIPRQQIDIAIQASIGSRIIARETIKALRKDVLSKCYGGD
ISRKRKLLEKQKAGKKRMRQYGNIEIPQSAFIAALKIGDE

Sequences:

>Translated_600_residues
MKNQKYIRNFSIIAHIDHGKSTLADRLIEHCGGLQAREMRTQVLDSMDIEKERGITIKAQTVRLVYKAKDGNTYYLNLMD
TPGHVDFAYEVSRSLAACEGSLLVVDSTQGVEAQTLANVYQAIEHDHEIVPVLNKIDLPASEPEQVKQQIEDIIGIDASE
AVLISAKSGIGIDLVLEAIVNKLHPPKESSTDILKALLVDSWYDPYLGVVILVRIIDGTLRKNMRIKMMATNSVYTVENV
GFFTPKKHISDVLYAGEIGFVTASIKQVADCKVGDTITDEKKPCEQALPGFKPNLPVVFCGLYPTDSSEFEHLKDSLAKL
RLNDASFEYEMESSSALGVGFRCGFLGLLHLEIIQERLSREFDLDLITTAPSVVYKIHMRYGESLEIHNPADLPDLQKIE
SMEEPWIKATIMVPDEFLGAVLSLCTEKRGVQLDHSYIANRAKIVYKLPLNEIVYDFYDRLKSCSKGYASFEWQIDVYEP
SELVKLGILVNGEVIDALSTIVHRSRAEQRGKVLCVRLKDLIPRQQIDIAIQASIGSRIIARETIKALRKDVLSKCYGGD
ISRKRKLLEKQKAGKKRMRQYGNIEIPQSAFIAALKIGDE
>Mature_600_residues
MKNQKYIRNFSIIAHIDHGKSTLADRLIEHCGGLQAREMRTQVLDSMDIEKERGITIKAQTVRLVYKAKDGNTYYLNLMD
TPGHVDFAYEVSRSLAACEGSLLVVDSTQGVEAQTLANVYQAIEHDHEIVPVLNKIDLPASEPEQVKQQIEDIIGIDASE
AVLISAKSGIGIDLVLEAIVNKLHPPKESSTDILKALLVDSWYDPYLGVVILVRIIDGTLRKNMRIKMMATNSVYTVENV
GFFTPKKHISDVLYAGEIGFVTASIKQVADCKVGDTITDEKKPCEQALPGFKPNLPVVFCGLYPTDSSEFEHLKDSLAKL
RLNDASFEYEMESSSALGVGFRCGFLGLLHLEIIQERLSREFDLDLITTAPSVVYKIHMRYGESLEIHNPADLPDLQKIE
SMEEPWIKATIMVPDEFLGAVLSLCTEKRGVQLDHSYIANRAKIVYKLPLNEIVYDFYDRLKSCSKGYASFEWQIDVYEP
SELVKLGILVNGEVIDALSTIVHRSRAEQRGKVLCVRLKDLIPRQQIDIAIQASIGSRIIARETIKALRKDVLSKCYGGD
ISRKRKLLEKQKAGKKRMRQYGNIEIPQSAFIAALKIGDE

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=599, Percent_Identity=48.914858096828, Blast_Score=599, Evalue=1e-171,
Organism=Homo sapiens, GI94966754, Length=235, Percent_Identity=34.468085106383, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=41.6107382550336, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI18390331, Length=163, Percent_Identity=35.5828220858896, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI25306283, Length=134, Percent_Identity=43.2835820895522, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI25306287, Length=134, Percent_Identity=43.2835820895522, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI19923640, Length=134, Percent_Identity=43.2835820895522, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI217272894, Length=140, Percent_Identity=36.4285714285714, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI217272892, Length=140, Percent_Identity=36.4285714285714, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI310132016, Length=114, Percent_Identity=41.2280701754386, Blast_Score=91, Evalue=5e-18,
Organism=Homo sapiens, GI310110807, Length=114, Percent_Identity=41.2280701754386, Blast_Score=91, Evalue=5e-18,
Organism=Homo sapiens, GI310123363, Length=114, Percent_Identity=41.2280701754386, Blast_Score=91, Evalue=5e-18,
Organism=Escherichia coli, GI1788922, Length=598, Percent_Identity=58.3612040133779, Blast_Score=714, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=531, Percent_Identity=27.3069679849341, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI1790835, Length=156, Percent_Identity=30.7692307692308, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=32.9032258064516, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789559, Length=226, Percent_Identity=28.3185840707965, Blast_Score=72, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=610, Percent_Identity=39.344262295082, Blast_Score=457, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI17556745, Length=454, Percent_Identity=25.9911894273128, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17506493, Length=189, Percent_Identity=36.5079365079365, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71988819, Length=137, Percent_Identity=35.7664233576642, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71988811, Length=137, Percent_Identity=35.7664233576642, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=143, Percent_Identity=34.2657342657343, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=35.3383458646617, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32566303, Length=325, Percent_Identity=23.6923076923077, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=598, Percent_Identity=44.1471571906354, Blast_Score=519, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6324707, Length=143, Percent_Identity=40.5594405594406, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=143, Percent_Identity=40.5594405594406, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6323098, Length=188, Percent_Identity=36.7021276595745, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=163, Percent_Identity=32.5153374233129, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324166, Length=153, Percent_Identity=33.9869281045752, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI78706572, Length=599, Percent_Identity=44.4073455759599, Blast_Score=528, Evalue=1e-150,
Organism=Drosophila melanogaster, GI28574573, Length=141, Percent_Identity=43.2624113475177, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24582462, Length=159, Percent_Identity=36.4779874213836, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24585709, Length=179, Percent_Identity=34.6368715083799, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24585711, Length=179, Percent_Identity=34.6368715083799, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24585713, Length=179, Percent_Identity=34.6368715083799, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=38.9261744966443, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21357743, Length=139, Percent_Identity=34.5323741007194, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI28572034, Length=223, Percent_Identity=30.9417040358744, Blast_Score=73, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 67231; Mature: 67231

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNQKYIRNFSIIAHIDHGKSTLADRLIEHCGGLQAREMRTQVLDSMDIEKERGITIKAQ
CCCCHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEE
TVRLVYKAKDGNTYYLNLMDTPGHVDFAYEVSRSLAACEGSLLVVDSTQGVEAQTLANVY
EEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHH
QAIEHDHEIVPVLNKIDLPASEPEQVKQQIEDIIGIDASEAVLISAKSGIGIDLVLEAIV
HHHHCCCCEEHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHH
NKLHPPKESSTDILKALLVDSWYDPYLGVVILVRIIDGTLRKNMRIKMMATNSVYTVENV
HHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCC
GFFTPKKHISDVLYAGEIGFVTASIKQVADCKVGDTITDEKKPCEQALPGFKPNLPVVFC
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEE
GLYPTDSSEFEHLKDSLAKLRLNDASFEYEMESSSALGVGFRCGFLGLLHLEIIQERLSR
ECCCCCCHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCC
EFDLDLITTAPSVVYKIHMRYGESLEIHNPADLPDLQKIESMEEPWIKATIMVPDEFLGA
CCCCEEEECCCHHEEEEEECCCCEEECCCCCCCCHHHHHHHHCCCCEEEEEECCHHHHHH
VLSLCTEKRGVQLDHSYIANRAKIVYKLPLNEIVYDFYDRLKSCSKGYASFEWQIDVYEP
HHHHHHHHCCCEECHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEEECC
SELVKLGILVNGEVIDALSTIVHRSRAEQRGKVLCVRLKDLIPRQQIDIAIQASIGSRII
HHHEEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCEEEEECCCCHHH
ARETIKALRKDVLSKCYGGDISRKRKLLEKQKAGKKRMRQYGNIEIPQSAFIAALKIGDE
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCC
>Mature Secondary Structure
MKNQKYIRNFSIIAHIDHGKSTLADRLIEHCGGLQAREMRTQVLDSMDIEKERGITIKAQ
CCCCHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEE
TVRLVYKAKDGNTYYLNLMDTPGHVDFAYEVSRSLAACEGSLLVVDSTQGVEAQTLANVY
EEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHH
QAIEHDHEIVPVLNKIDLPASEPEQVKQQIEDIIGIDASEAVLISAKSGIGIDLVLEAIV
HHHHCCCCEEHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHH
NKLHPPKESSTDILKALLVDSWYDPYLGVVILVRIIDGTLRKNMRIKMMATNSVYTVENV
HHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCC
GFFTPKKHISDVLYAGEIGFVTASIKQVADCKVGDTITDEKKPCEQALPGFKPNLPVVFC
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEE
GLYPTDSSEFEHLKDSLAKLRLNDASFEYEMESSSALGVGFRCGFLGLLHLEIIQERLSR
ECCCCCCHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCC
EFDLDLITTAPSVVYKIHMRYGESLEIHNPADLPDLQKIESMEEPWIKATIMVPDEFLGA
CCCCEEEECCCHHEEEEEECCCCEEECCCCCCCCHHHHHHHHCCCCEEEEEECCHHHHHH
VLSLCTEKRGVQLDHSYIANRAKIVYKLPLNEIVYDFYDRLKSCSKGYASFEWQIDVYEP
HHHHHHHHCCCEECHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEEECC
SELVKLGILVNGEVIDALSTIVHRSRAEQRGKVLCVRLKDLIPRQQIDIAIQASIGSRII
HHHEEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCEEEEECCCCHHH
ARETIKALRKDVLSKCYGGDISRKRKLLEKQKAGKKRMRQYGNIEIPQSAFIAALKIGDE
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA