Click here to switch to the map view.

The map label for this gene is pdhB [H]

Identifier: 157825477

GI number: 157825477

Start: 354594

End: 355574

Strand: Direct

Name: pdhB [H]

Synonym: A1C_01890

Alternate gene names: 157825477

Gene position: 354594-355574 (Clockwise)

Preceding gene: 157825476

Following gene: 157825479

Centisome position: 28.8

GC content: 39.25

Gene sequence:

>981_bases
ATGCAAATAACGGTACGTGAAGCGTTGCGTGATGCGATGCGAGAAGAGATGATAAGAGACGATAAAGTTTTTGTCATGGG
TGAGGAAGTTGCAGAGTATCAAGGAGCTTACAAGGTAACTCAAGGATTACTAGAGCAATTTGGTCCTAAGAGAGTAATTG
ATACGCCAATAACGGAATATGGTTTTGCAGGGCTTGCAGTCGGAGCAGCTTTTGCAGGACTGCGCCCGATCGTGGAGTTT
ATGACCTTTAACTTTGCTATGCAAGCATTCGATCATATAGTTAATTCAGCTGCAAAAACGCATTACATGTCAGGCGGGCA
GGTCAAATGTCCGATAGTATTTAGAGGACCAAACGGAGCAGCAAGTAGAGTAGCCGCACAACATAGCCAAAATTATACAG
CTTGTTATTCTCACATTCCAGGGTTAAAAGTAGTAGCTCCTTATGGTGCAGAAGATCATAAAGGGCTTATGCTTACAGCT
ATTAGGGACAATAATCCCGTTGTTTTTTTAGAAAATGAAATTTTATACGGTCATAGTTTTTACGTACCGGAAACAATCGA
GCCTATACCCTATGGTCAGGCAAAAATTTTAAAAGAAGGTAGTAGCGTCACTATAGTCACTTTTTCGATTCAAGTAAAAC
TTGCCTTAGATGCCGCAAATGTTTTACATGGTGATAATATTGATTGTGAAGTTATTGATCTGCGCACTATTAAACCGCTT
GATACGGATACAATAATAGAATCGGTGAAAAAAACTAATCGTTTAGTTGTAGTAGAAGAAGGGTGGTTTTTTGCAGGTGT
TGGAGCAAGTATTGCTTCTATTGTTATGAAAGAAGCATTCGATTATTTAGATGCTCCGATAGAGATTGTAAGCGGTAAAG
ACGTACCACTTCCTTATGCCATTAATTTAGAAAAATTAGCTCTGCCGAGCGAGAGCGACGTAATAGAAGCAGTAAAGAAA
GTTTGTTATTATAGTGTTTAG

Upstream 100 bases:

>100_bases
TATGTCATTCCTGCAAAAGCAGGAATGACATAGATATCCACGCAACAATACCATAGGCACTATGTGAATGACACAAACTT
TAATCAAATAAAAAAGAAAA

Downstream 100 bases:

>100_bases
GGGTATTTAGGTTATTCCTGAGACATTGTGGTATGACCTGATGTCATGCCCGTGTAGGCGGGAATCCAGCAAAACCTATA
ACAACTAGTTTTTAGGTTTA

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MQITVREALRDAMREEMIRDDKVFVMGEEVAEYQGAYKVTQGLLEQFGPKRVIDTPITEYGFAGLAVGAAFAGLRPIVEF
MTFNFAMQAFDHIVNSAAKTHYMSGGQVKCPIVFRGPNGAASRVAAQHSQNYTACYSHIPGLKVVAPYGAEDHKGLMLTA
IRDNNPVVFLENEILYGHSFYVPETIEPIPYGQAKILKEGSSVTIVTFSIQVKLALDAANVLHGDNIDCEVIDLRTIKPL
DTDTIIESVKKTNRLVVVEEGWFFAGVGASIASIVMKEAFDYLDAPIEIVSGKDVPLPYAINLEKLALPSESDVIEAVKK
VCYYSV

Sequences:

>Translated_326_residues
MQITVREALRDAMREEMIRDDKVFVMGEEVAEYQGAYKVTQGLLEQFGPKRVIDTPITEYGFAGLAVGAAFAGLRPIVEF
MTFNFAMQAFDHIVNSAAKTHYMSGGQVKCPIVFRGPNGAASRVAAQHSQNYTACYSHIPGLKVVAPYGAEDHKGLMLTA
IRDNNPVVFLENEILYGHSFYVPETIEPIPYGQAKILKEGSSVTIVTFSIQVKLALDAANVLHGDNIDCEVIDLRTIKPL
DTDTIIESVKKTNRLVVVEEGWFFAGVGASIASIVMKEAFDYLDAPIEIVSGKDVPLPYAINLEKLALPSESDVIEAVKK
VCYYSV
>Mature_326_residues
MQITVREALRDAMREEMIRDDKVFVMGEEVAEYQGAYKVTQGLLEQFGPKRVIDTPITEYGFAGLAVGAAFAGLRPIVEF
MTFNFAMQAFDHIVNSAAKTHYMSGGQVKCPIVFRGPNGAASRVAAQHSQNYTACYSHIPGLKVVAPYGAEDHKGLMLTA
IRDNNPVVFLENEILYGHSFYVPETIEPIPYGQAKILKEGSSVTIVTFSIQVKLALDAANVLHGDNIDCEVIDLRTIKPL
DTDTIIESVKKTNRLVVVEEGWFFAGVGASIASIVMKEAFDYLDAPIEIVSGKDVPLPYAINLEKLALPSESDVIEAVKK
VCYYSV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI156564403, Length=325, Percent_Identity=57.5384615384615, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI291084858, Length=325, Percent_Identity=54.1538461538462, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI4557353, Length=328, Percent_Identity=37.5, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI34101272, Length=328, Percent_Identity=37.5, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI225637461, Length=233, Percent_Identity=31.3304721030043, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI225637459, Length=233, Percent_Identity=31.3304721030043, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI225637463, Length=233, Percent_Identity=31.3304721030043, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI205277463, Length=242, Percent_Identity=28.5123966942149, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI4507521, Length=242, Percent_Identity=28.5123966942149, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1786622, Length=276, Percent_Identity=23.9130434782609, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17538422, Length=323, Percent_Identity=60.6811145510836, Blast_Score=396, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17506935, Length=326, Percent_Identity=40.7975460122699, Blast_Score=209, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6319698, Length=326, Percent_Identity=60.4294478527607, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI21358145, Length=325, Percent_Identity=57.8461538461538, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24650940, Length=325, Percent_Identity=57.8461538461538, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI160714828, Length=323, Percent_Identity=35.6037151702786, Blast_Score=205, Evalue=4e-53,
Organism=Drosophila melanogaster, GI160714832, Length=323, Percent_Identity=35.6037151702786, Blast_Score=204, Evalue=5e-53,
Organism=Drosophila melanogaster, GI24650943, Length=91, Percent_Identity=68.1318681318681, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24650945, Length=91, Percent_Identity=68.1318681318681, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24645119, Length=252, Percent_Identity=29.7619047619048, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45551847, Length=252, Percent_Identity=29.7619047619048, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45550715, Length=252, Percent_Identity=29.7619047619048, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 35791; Mature: 35791

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQITVREALRDAMREEMIRDDKVFVMGEEVAEYQGAYKVTQGLLEQFGPKRVIDTPITEY
CEEEHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHH
GFAGLAVGAAFAGLRPIVEFMTFNFAMQAFDHIVNSAAKTHYMSGGQVKCPIVFRGPNGA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCH
ASRVAAQHSQNYTACYSHIPGLKVVAPYGAEDHKGLMLTAIRDNNPVVFLENEILYGHSF
HHHHHHHHCCCCHHHHHCCCCCEEEECCCCCCCCCEEEEEEECCCCEEEEECCEEECCEE
YVPETIEPIPYGQAKILKEGSSVTIVTFSIQVKLALDAANVLHGDNIDCEVIDLRTIKPL
ECCCCCCCCCCCHHHHEECCCCEEEEEEEEEEEEEEEHHHHHCCCCCCEEEEEEEECCCC
DTDTIIESVKKTNRLVVVEEGWFFAGVGASIASIVMKEAFDYLDAPIEIVSGKDVPLPYA
CHHHHHHHHHHCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEE
INLEKLALPSESDVIEAVKKVCYYSV
ECHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQITVREALRDAMREEMIRDDKVFVMGEEVAEYQGAYKVTQGLLEQFGPKRVIDTPITEY
CEEEHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHH
GFAGLAVGAAFAGLRPIVEFMTFNFAMQAFDHIVNSAAKTHYMSGGQVKCPIVFRGPNGA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCH
ASRVAAQHSQNYTACYSHIPGLKVVAPYGAEDHKGLMLTAIRDNNPVVFLENEILYGHSF
HHHHHHHHCCCCHHHHHCCCCCEEEECCCCCCCCCEEEEEEECCCCEEEEECCEEECCEE
YVPETIEPIPYGQAKILKEGSSVTIVTFSIQVKLALDAANVLHGDNIDCEVIDLRTIKPL
ECCCCCCCCCCCHHHHEECCCCEEEEEEEEEEEEEEEHHHHHCCCCCCEEEEEEEECCCC
DTDTIIESVKKTNRLVVVEEGWFFAGVGASIASIVMKEAFDYLDAPIEIVSGKDVPLPYA
CHHHHHHHHHHCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEE
INLEKLALPSESDVIEAVKKVCYYSV
ECHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]