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The map label for this gene is proP [H]

Identifier: 157825237

GI number: 157825237

Start: 98049

End: 99305

Strand: Direct

Name: proP [H]

Synonym: A1C_00580

Alternate gene names: 157825237

Gene position: 98049-99305 (Clockwise)

Preceding gene: 157825236

Following gene: 157825239

Centisome position: 7.96

GC content: 32.94

Gene sequence:

>1257_bases
ATGAATCGCAGTAAGCTAGTATTTTCAAGCGGTATCGCTAACACTTTTGAATGGTATGATTATGTATTATTCGGTTATTT
TGCACCCATAATAGGAGCAAAATTTTTCCCTAATTATGATGCTAATACTGCTTTACTGCAAGCTTTTTTAGTTTTTGCTA
TAGGTTATTTGGCAAGACCGTTGGGTGGCATATTTTTTGGTGTTATTGGTGATAGATTGGGACGCAAAGTAGCCTTAACT
AGTGCGTTATTTTGCATGTCTTCCCCAACCGTATTAATAGGAATATTACCTACGTACCATACTATAGGTATAACTGCGAC
TATATTAATGATTTGTGTACGCATTCTACAAGGATTATCAATGGGAGGAGCTTTGACCGGTTCTATTTCTTTTGTTATTG
AACATACTAGCCATAAACACCGAGGCTTTACCGGTAGTATTTCAATGTCTAGTATATGCTCCGGTTTATTGCTTGGTTCT
TTTGTTTCTTATATTGTTAAGAATATCCTAACAGCTTCACAATTTGATAGTTTTGGCTGGAGAATACCTTTTTTACTTGG
ATTTTTTATTCTTTTTGCAGCATTTTATATTAAAAAAAATACACACGAAACACCGGATTTTAAAAATCTAAGAGAGCAGA
AAAAGATTTTACAATCACCGTTAAAAAGAGTCATTACCAATCATTGGTTTGATATATTAATCTCGATTTTTATCAATGCT
CCCGGTTCGATAATATTCTATCTAACTACAATTTACTTGGTATCGTTTTTAAAAATTAGCCGTAACTTTACTGAAAATGA
GGTAAATAGCTTCGCTAGTGTATGTTATGTGATTATGATAGCCGTAACTTTATTAAGCGGTTATCTATCCGATATTATAG
GTCGCAGGAAAATTTTTGTAATTAATCTGATAATAATTATTGTAACAACACCCTTTTTATTTCATAATTTTGAGAGCGGG
GATTTCACAAGTGTAATTATTTCACAATTTATACTTGCTATACTGGCGGCTAGCTATATCGGTCCTGAACCGGCATTACA
AGCAGAATTTTATCCTACAAATATACGTAACACTGCTCTCTCTATTTCATATAACACTGCTACAAGTATTTTTGGTGGTA
CTACGCCTCTTGTCTTTGAATACTTAGTACAAAAAACAGGGAATGTCACCTCGGCAATTTATTATGTCATATTAAGCTGT
ATTTTTGCATTGATCGCCTTATCTTTTTACAAAAATAGAAGTTTAGATACAATATGA

Upstream 100 bases:

>100_bases
AACGAGTTTCCTGATAAAAATACACTTCATGGAGCAGTAATAATAATTCCTGCCACCTTATTTATAATATACTCCGAAAA
AAAAGCTATGAGTAGAAAAC

Downstream 100 bases:

>100_bases
AAAAATTACAAAACCTGCAAACATAGTTGAGTTTAATGATAAAATTGTAGTCTTGACACCTAAGAGTTCTTACTATGGCA
ATGCTTACGAACACAAAATA

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MNRSKLVFSSGIANTFEWYDYVLFGYFAPIIGAKFFPNYDANTALLQAFLVFAIGYLARPLGGIFFGVIGDRLGRKVALT
SALFCMSSPTVLIGILPTYHTIGITATILMICVRILQGLSMGGALTGSISFVIEHTSHKHRGFTGSISMSSICSGLLLGS
FVSYIVKNILTASQFDSFGWRIPFLLGFFILFAAFYIKKNTHETPDFKNLREQKKILQSPLKRVITNHWFDILISIFINA
PGSIIFYLTTIYLVSFLKISRNFTENEVNSFASVCYVIMIAVTLLSGYLSDIIGRRKIFVINLIIIIVTTPFLFHNFESG
DFTSVIISQFILAILAASYIGPEPALQAEFYPTNIRNTALSISYNTATSIFGGTTPLVFEYLVQKTGNVTSAIYYVILSC
IFALIALSFYKNRSLDTI

Sequences:

>Translated_418_residues
MNRSKLVFSSGIANTFEWYDYVLFGYFAPIIGAKFFPNYDANTALLQAFLVFAIGYLARPLGGIFFGVIGDRLGRKVALT
SALFCMSSPTVLIGILPTYHTIGITATILMICVRILQGLSMGGALTGSISFVIEHTSHKHRGFTGSISMSSICSGLLLGS
FVSYIVKNILTASQFDSFGWRIPFLLGFFILFAAFYIKKNTHETPDFKNLREQKKILQSPLKRVITNHWFDILISIFINA
PGSIIFYLTTIYLVSFLKISRNFTENEVNSFASVCYVIMIAVTLLSGYLSDIIGRRKIFVINLIIIIVTTPFLFHNFESG
DFTSVIISQFILAILAASYIGPEPALQAEFYPTNIRNTALSISYNTATSIFGGTTPLVFEYLVQKTGNVTSAIYYVILSC
IFALIALSFYKNRSLDTI
>Mature_418_residues
MNRSKLVFSSGIANTFEWYDYVLFGYFAPIIGAKFFPNYDANTALLQAFLVFAIGYLARPLGGIFFGVIGDRLGRKVALT
SALFCMSSPTVLIGILPTYHTIGITATILMICVRILQGLSMGGALTGSISFVIEHTSHKHRGFTGSISMSSICSGLLLGS
FVSYIVKNILTASQFDSFGWRIPFLLGFFILFAAFYIKKNTHETPDFKNLREQKKILQSPLKRVITNHWFDILISIFINA
PGSIIFYLTTIYLVSFLKISRNFTENEVNSFASVCYVIMIAVTLLSGYLSDIIGRRKIFVINLIIIIVTTPFLFHNFESG
DFTSVIISQFILAILAASYIGPEPALQAEFYPTNIRNTALSISYNTATSIFGGTTPLVFEYLVQKTGNVTSAIYYVILSC
IFALIALSFYKNRSLDTI

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=410, Percent_Identity=28.780487804878, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1789941, Length=415, Percent_Identity=31.566265060241, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1788292, Length=413, Percent_Identity=27.6029055690073, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1788942, Length=410, Percent_Identity=30.2439024390244, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI87082404, Length=400, Percent_Identity=23.5, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI87082231, Length=184, Percent_Identity=26.6304347826087, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46440; Mature: 46440

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00189 LIPOYL ; PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSKLVFSSGIANTFEWYDYVLFGYFAPIIGAKFFPNYDANTALLQAFLVFAIGYLARP
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LGGIFFGVIGDRLGRKVALTSALFCMSSPTVLIGILPTYHTIGITATILMICVRILQGLS
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCC
MGGALTGSISFVIEHTSHKHRGFTGSISMSSICSGLLLGSFVSYIVKNILTASQFDSFGW
CCCCHHCHHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RIPFLLGFFILFAAFYIKKNTHETPDFKNLREQKKILQSPLKRVITNHWFDILISIFINA
CHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGSIIFYLTTIYLVSFLKISRNFTENEVNSFASVCYVIMIAVTLLSGYLSDIIGRRKIFV
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
INLIIIIVTTPFLFHNFESGDFTSVIISQFILAILAASYIGPEPALQAEFYPTNIRNTAL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
SISYNTATSIFGGTTPLVFEYLVQKTGNVTSAIYYVILSCIFALIALSFYKNRSLDTI
EEEECCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNRSKLVFSSGIANTFEWYDYVLFGYFAPIIGAKFFPNYDANTALLQAFLVFAIGYLARP
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LGGIFFGVIGDRLGRKVALTSALFCMSSPTVLIGILPTYHTIGITATILMICVRILQGLS
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCC
MGGALTGSISFVIEHTSHKHRGFTGSISMSSICSGLLLGSFVSYIVKNILTASQFDSFGW
CCCCHHCHHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RIPFLLGFFILFAAFYIKKNTHETPDFKNLREQKKILQSPLKRVITNHWFDILISIFINA
CHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGSIIFYLTTIYLVSFLKISRNFTENEVNSFASVCYVIMIAVTLLSGYLSDIIGRRKIFV
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
INLIIIIVTTPFLFHNFESGDFTSVIISQFILAILAASYIGPEPALQAEFYPTNIRNTAL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
SISYNTATSIFGGTTPLVFEYLVQKTGNVTSAIYYVILSCIFALIALSFYKNRSLDTI
EEEECCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]