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The map label for this gene is mscM [H]

Identifier: 157825201

GI number: 157825201

Start: 62087

End: 63241

Strand: Direct

Name: mscM [H]

Synonym: A1C_00400

Alternate gene names: 157825201

Gene position: 62087-63241 (Clockwise)

Preceding gene: 157825198

Following gene: 157825202

Centisome position: 5.04

GC content: 27.36

Gene sequence:

>1155_bases
ATGGATCTATATCATACCGCAAGTACTGAGTTAGTATTATTGTTTGTTATGTTAGTATCTCTTATACCGGTGATATTTCT
AATTAAAAGATTAATTTTTATCCCTGTACAAAATTATTTAACTAGACATCATGATTATAAGTATGAAAGGATACTAAAAA
AATATCCTATATTTCGTTATTTATTACATACTTTATTAGCACTTTACTTTGTAGGTTGGGGAAATATTTTCCATATTACT
TCTTTTAAAGCACATGTATTACTTGGAATCAAAGATATTATAGTAATCTTATATACTAGTATATCAGTGACGATGTTATT
ATTAACGCTTATAGATGCTTTTGCAGATCTGTATCACGATAGGATCAAAGTTGTTGCAAAAAATGCACCGCTTAGCTTAT
ATTTTCAAATATTGAAAATTATTGTTGTAGTTATTGCAGTAATGGTAACTATTTCATATATGTTAAATATTTCGCTTAGT
ACATTCTTAACAAGTTTAGGTGCAGCCGCAGCTCTTTTAACATTTGTTTTCAAAGATACCGTTTTAGGGTTACTTGCAAG
TTTGCAATTAACTACTCAAGAAATTATTAATATTGGAGACTGGGTACGTATAGGAAAAATTGAGGGAACAGTTGAAAAAA
TTACTATTTCCGTAGTAACAATTAGGAATTTTGATCAATCTATTTCTACAGTTCCTACTTACAGCATTTTAAATTCTAAT
GTAACTAATTATAGAGGAATTAGTGAATCAGGAGCACGAAGGGTAAAGAGAGTATTTAATATTAATATGGCAACTATTAA
CTTCTGTGATGCGACTATTTTAAAAGAACTAAAAAAATCTCCTCATATATCAAAGGATGTAATAGATAAAATCACTATTG
ATAAAGAACAGAAAGATTTAACAAACATTAAGCTGTTCAAATTATATATTCAGGAATATCTAAAAAATAATCCGGCAGTT
TATACTGAAGGATTTACTTTTTTAGTAAGACAACTTGAACCTACAATTACCGGGTTGCCTATAGAAATATATATATTCAT
TAAAGAAGTGAATTTAGTAGGTTATGAAAATATACAAGACAATATTTCTGAACATTTAATTTCTATACTTCCTGAATTTA
AATTAAAAATATTTCAGAATATAGGAGTGGTATAA

Upstream 100 bases:

>100_bases
TTCTTTTGAGTTAAAATTATTGATAAAAATAAAATGCTTAGTCATAATAAAAAAGATTTATATTTATAATAATAATGATA
GGTAAAATATGCAATATTTA

Downstream 100 bases:

>100_bases
TATCCCTGAAGTTAAATTTGTATACAAGATGGAAGCCGCACTTCATCTTGTATATATACTACTTGATTATTTTCTCTAAA
TGCTTTAGAACTTAAGGCTT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MDLYHTASTELVLLFVMLVSLIPVIFLIKRLIFIPVQNYLTRHHDYKYERILKKYPIFRYLLHTLLALYFVGWGNIFHIT
SFKAHVLLGIKDIIVILYTSISVTMLLLTLIDAFADLYHDRIKVVAKNAPLSLYFQILKIIVVVIAVMVTISYMLNISLS
TFLTSLGAAAALLTFVFKDTVLGLLASLQLTTQEIINIGDWVRIGKIEGTVEKITISVVTIRNFDQSISTVPTYSILNSN
VTNYRGISESGARRVKRVFNINMATINFCDATILKELKKSPHISKDVIDKITIDKEQKDLTNIKLFKLYIQEYLKNNPAV
YTEGFTFLVRQLEPTITGLPIEIYIFIKEVNLVGYENIQDNISEHLISILPEFKLKIFQNIGVV

Sequences:

>Translated_384_residues
MDLYHTASTELVLLFVMLVSLIPVIFLIKRLIFIPVQNYLTRHHDYKYERILKKYPIFRYLLHTLLALYFVGWGNIFHIT
SFKAHVLLGIKDIIVILYTSISVTMLLLTLIDAFADLYHDRIKVVAKNAPLSLYFQILKIIVVVIAVMVTISYMLNISLS
TFLTSLGAAAALLTFVFKDTVLGLLASLQLTTQEIINIGDWVRIGKIEGTVEKITISVVTIRNFDQSISTVPTYSILNSN
VTNYRGISESGARRVKRVFNINMATINFCDATILKELKKSPHISKDVIDKITIDKEQKDLTNIKLFKLYIQEYLKNNPAV
YTEGFTFLVRQLEPTITGLPIEIYIFIKEVNLVGYENIQDNISEHLISILPEFKLKIFQNIGVV
>Mature_384_residues
MDLYHTASTELVLLFVMLVSLIPVIFLIKRLIFIPVQNYLTRHHDYKYERILKKYPIFRYLLHTLLALYFVGWGNIFHIT
SFKAHVLLGIKDIIVILYTSISVTMLLLTLIDAFADLYHDRIKVVAKNAPLSLYFQILKIIVVVIAVMVTISYMLNISLS
TFLTSLGAAAALLTFVFKDTVLGLLASLQLTTQEIINIGDWVRIGKIEGTVEKITISVVTIRNFDQSISTVPTYSILNSN
VTNYRGISESGARRVKRVFNINMATINFCDATILKELKKSPHISKDVIDKITIDKEQKDLTNIKLFKLYIQEYLKNNPAV
YTEGFTFLVRQLEPTITGLPIEIYIFIKEVNLVGYENIQDNISEHLISILPEFKLKIFQNIGVV

Specific function: Mechanosensitive channel of miniconductance that confers protection against mild hypoosmotic shock. Overexpression confers protection against severe shocks [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786791, Length=315, Percent_Identity=33.015873015873, Blast_Score=185, Evalue=5e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 43858; Mature: 43858

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLYHTASTELVLLFVMLVSLIPVIFLIKRLIFIPVQNYLTRHHDYKYERILKKYPIFRY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHH
LLHTLLALYFVGWGNIFHITSFKAHVLLGIKDIIVILYTSISVTMLLLTLIDAFADLYHD
HHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIKVVAKNAPLSLYFQILKIIVVVIAVMVTISYMLNISLSTFLTSLGAAAALLTFVFKDT
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLGLLASLQLTTQEIINIGDWVRIGKIEGTVEKITISVVTIRNFDQSISTVPTYSILNSN
HHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEEEEEEECCCCHHHCCCCHHHHCCC
VTNYRGISESGARRVKRVFNINMATINFCDATILKELKKSPHISKDVIDKITIDKEQKDL
CCCCCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHH
TNIKLFKLYIQEYLKNNPAVYTEGFTFLVRQLEPTITGLPIEIYIFIKEVNLVGYENIQD
HHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCHHHHH
NISEHLISILPEFKLKIFQNIGVV
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDLYHTASTELVLLFVMLVSLIPVIFLIKRLIFIPVQNYLTRHHDYKYERILKKYPIFRY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHH
LLHTLLALYFVGWGNIFHITSFKAHVLLGIKDIIVILYTSISVTMLLLTLIDAFADLYHD
HHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIKVVAKNAPLSLYFQILKIIVVVIAVMVTISYMLNISLSTFLTSLGAAAALLTFVFKDT
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLGLLASLQLTTQEIINIGDWVRIGKIEGTVEKITISVVTIRNFDQSISTVPTYSILNSN
HHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEEEEEEECCCCHHHCCCCHHHHCCC
VTNYRGISESGARRVKRVFNINMATINFCDATILKELKKSPHISKDVIDKITIDKEQKDL
CCCCCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHH
TNIKLFKLYIQEYLKNNPAVYTEGFTFLVRQLEPTITGLPIEIYIFIKEVNLVGYENIQD
HHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCHHHHH
NISEHLISILPEFKLKIFQNIGVV
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 7813889 [H]