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The map label for this gene is dus [H]

Identifier: 157825185

GI number: 157825185

Start: 48532

End: 49515

Strand: Reverse

Name: dus [H]

Synonym: A1C_00320

Alternate gene names: 157825185

Gene position: 49515-48532 (Counterclockwise)

Preceding gene: 157825186

Following gene: 157825184

Centisome position: 4.02

GC content: 40.14

Gene sequence:

>984_bases
ATGATCAAAATCGGTAATATAGAACTTTCTTCAAATGTAATGCTTGCTCCAATGTCTGATATTACGGACTTAGAATTTAG
AAAATTGGTAAAAAGATTTGGGGCAGGGCTTGTAGTTTCTGAGATGATAGCAAGTCGTGCAATGGTTGTGAAATCTAGGC
AGTCAATGCAAAAATGTGCTATCATGCATGACGATCCGACAAGTGCATGCGTGCAGCTAGCAGGCTGTGAGCCTAATGTA
ATAGCTGAAGCTGCTAAAATGAACGAGGATATGGGAGCGAAAATTATCGATCTTAATTTCGGCTGTCCAGCGAAAAAAGT
AGTAGGCGGTTATGCAGGTGCGGCTCTAATGAGTGATGAACAGCTGGCATCTAAGATTTTTGAAGCTACCGTTAAAGCGG
TGAAGATTCCAATAACGGTTAAAATGCGTATGGGGTGGGATGATAATACGAAAAACGCTCCGACTTTATCTAAAATTGCT
GAAAACTCAGGGATTCAAATGGTCACCGTTCACGGTAGAACGAGATGTCAGTTTTACTCCGGTAATGCCGATTGGAATTT
TATAAGATCAGTTAAAGAAGCAGTAAAAATTCCGGTTATTGCTAACGGTGACATCACTAATTTTGCTAAAGCAAAAGAAG
CCCTGCAGAAATCCGGTGCAGATGGTATTATGGTCGGTAGAGGAGCATACGGCAAACCTTGGATTATTTCACAAATTGAT
CATTACCTTAAAACCGGTGAAAGAAAACCTGATCCATCTATAGCAGAGCAGCTAGATATCGTAACCGAGCATTGTGAGGC
AATACTTGATTATTATGGCAAATCTGTTGGCATACCTATTGCTCGTAAGCATATTAGTTGGTATAGTAGCGGTTTACCAA
GTTCAGCAGAGTTTAGAGGTGCCGTTAATTTGATGCATGACCCCGCAGAGTTAAAAGAGAAAATTGCAGAATTTTATACG
AGCATTATGGATACGAATAAATGA

Upstream 100 bases:

>100_bases
TATAATAGATTAATCGGCTTTTGTCTATTCTTAATTAGTAATATTAGACAAAAATACTTAATTATGATATCATGAGTGTT
TATTTTTATAACGGTTTTTT

Downstream 100 bases:

>100_bases
AAAGAATTTTAAGTTTTATTTTTATAATATTGTTTTTTAATTTAAGTTATGCGGATGAAAAAGCAGCGATTATAACTGAT
TATAAACCGGTATTTTTGCC

Product: nifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MIKIGNIELSSNVMLAPMSDITDLEFRKLVKRFGAGLVVSEMIASRAMVVKSRQSMQKCAIMHDDPTSACVQLAGCEPNV
IAEAAKMNEDMGAKIIDLNFGCPAKKVVGGYAGAALMSDEQLASKIFEATVKAVKIPITVKMRMGWDDNTKNAPTLSKIA
ENSGIQMVTVHGRTRCQFYSGNADWNFIRSVKEAVKIPVIANGDITNFAKAKEALQKSGADGIMVGRGAYGKPWIISQID
HYLKTGERKPDPSIAEQLDIVTEHCEAILDYYGKSVGIPIARKHISWYSSGLPSSAEFRGAVNLMHDPAELKEKIAEFYT
SIMDTNK

Sequences:

>Translated_327_residues
MIKIGNIELSSNVMLAPMSDITDLEFRKLVKRFGAGLVVSEMIASRAMVVKSRQSMQKCAIMHDDPTSACVQLAGCEPNV
IAEAAKMNEDMGAKIIDLNFGCPAKKVVGGYAGAALMSDEQLASKIFEATVKAVKIPITVKMRMGWDDNTKNAPTLSKIA
ENSGIQMVTVHGRTRCQFYSGNADWNFIRSVKEAVKIPVIANGDITNFAKAKEALQKSGADGIMVGRGAYGKPWIISQID
HYLKTGERKPDPSIAEQLDIVTEHCEAILDYYGKSVGIPIARKHISWYSSGLPSSAEFRGAVNLMHDPAELKEKIAEFYT
SIMDTNK
>Mature_327_residues
MIKIGNIELSSNVMLAPMSDITDLEFRKLVKRFGAGLVVSEMIASRAMVVKSRQSMQKCAIMHDDPTSACVQLAGCEPNV
IAEAAKMNEDMGAKIIDLNFGCPAKKVVGGYAGAALMSDEQLASKIFEATVKAVKIPITVKMRMGWDDNTKNAPTLSKIA
ENSGIQMVTVHGRTRCQFYSGNADWNFIRSVKEAVKIPVIANGDITNFAKAKEALQKSGADGIMVGRGAYGKPWIISQID
HYLKTGERKPDPSIAEQLDIVTEHCEAILDYYGKSVGIPIARKHISWYSSGLPSSAEFRGAVNLMHDPAELKEKIAEFYT
SIMDTNK

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=290, Percent_Identity=30.6896551724138, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI239788462, Length=290, Percent_Identity=30.6896551724138, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI40807366, Length=276, Percent_Identity=30.7971014492754, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI31742496, Length=253, Percent_Identity=29.2490118577075, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI8923374, Length=257, Percent_Identity=27.6264591439689, Blast_Score=85, Evalue=9e-17,
Organism=Escherichia coli, GI1789660, Length=322, Percent_Identity=41.9254658385093, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1788462, Length=260, Percent_Identity=31.5384615384615, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI145693211, Length=256, Percent_Identity=30.078125, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17543114, Length=287, Percent_Identity=29.9651567944251, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17507177, Length=236, Percent_Identity=30.5084745762712, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25144369, Length=225, Percent_Identity=28.8888888888889, Blast_Score=98, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6323433, Length=313, Percent_Identity=25.2396166134185, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323560, Length=264, Percent_Identity=28.030303030303, Blast_Score=96, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323437, Length=227, Percent_Identity=25.1101321585903, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24585320, Length=297, Percent_Identity=29.6296296296296, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24580595, Length=257, Percent_Identity=31.1284046692607, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI19920448, Length=257, Percent_Identity=31.1284046692607, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI19921524, Length=251, Percent_Identity=25.0996015936255, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI45549423, Length=241, Percent_Identity=26.5560165975104, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35696; Mature: 35696

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKIGNIELSSNVMLAPMSDITDLEFRKLVKRFGAGLVVSEMIASRAMVVKSRQSMQKCA
CEEECCEEECCCEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IMHDDPTSACVQLAGCEPNVIAEAAKMNEDMGAKIIDLNFGCPAKKVVGGYAGAALMSDE
CCCCCCHHHHHEECCCCCHHHHHHHHCCCCCCCEEEEECCCCCHHHHHCCHHHHHHCCHH
QLASKIFEATVKAVKIPITVKMRMGWDDNTKNAPTLSKIAENSGIQMVTVHGRTRCQFYS
HHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCCCHHHHHHCCCCEEEEEECCCEEEEEEC
GNADWNFIRSVKEAVKIPVIANGDITNFAKAKEALQKSGADGIMVGRGAYGKPWIISQID
CCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHH
HYLKTGERKPDPSIAEQLDIVTEHCEAILDYYGKSVGIPIARKHISWYSSGLPSSAEFRG
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHH
AVNLMHDPAELKEKIAEFYTSIMDTNK
HHHHCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIKIGNIELSSNVMLAPMSDITDLEFRKLVKRFGAGLVVSEMIASRAMVVKSRQSMQKCA
CEEECCEEECCCEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IMHDDPTSACVQLAGCEPNVIAEAAKMNEDMGAKIIDLNFGCPAKKVVGGYAGAALMSDE
CCCCCCHHHHHEECCCCCHHHHHHHHCCCCCCCEEEEECCCCCHHHHHCCHHHHHHCCHH
QLASKIFEATVKAVKIPITVKMRMGWDDNTKNAPTLSKIAENSGIQMVTVHGRTRCQFYS
HHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCCCHHHHHHCCCCEEEEEECCCEEEEEEC
GNADWNFIRSVKEAVKIPVIANGDITNFAKAKEALQKSGADGIMVGRGAYGKPWIISQID
CCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHH
HYLKTGERKPDPSIAEQLDIVTEHCEAILDYYGKSVGIPIARKHISWYSSGLPSSAEFRG
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHH
AVNLMHDPAELKEKIAEFYTSIMDTNK
HHHHCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]