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The map label for this gene is recF [H]

Identifier: 157825165

GI number: 157825165

Start: 29260

End: 30342

Strand: Direct

Name: recF [H]

Synonym: A1C_00220

Alternate gene names: 157825165

Gene position: 29260-30342 (Clockwise)

Preceding gene: 157825164

Following gene: 157825166

Centisome position: 2.38

GC content: 31.39

Gene sequence:

>1083_bases
ATGAAAAATATCTTCCTACATTCCTTAAGCCTCGCAAATTATCGTAACTTTAAAAATCTTGAATTAAAAACAGATAATAC
ACCAATAATTTTAATAGGTGAAAACGGTAGTGGTAAAACTAATATTTTAGAAGCTATATCGTTGTTTTATCCAGGTAGAG
GACTGCGGTCGGCTAAACTTGACGATATATGCAAAGCTTCAGAAGATTATTGCATCGTTAAAGCTTTATTACAAAGTCAA
TTAGGACTAGCAGAGTTTAGTACGCATATCAAACGCAACTCAAATAGGCGAATAACCGAATATAATGAAAGTAAAATAGC
TAATAATGAGTTAAGTAAATTCACTAGTATGGTATGGCTGACTCCACAAATGGAAGGAATCTTTACAAGCGGTAGCAGTG
ATAGAAGAAAATTTCTCGATAGAATAGTTTATAATTTTTATCCAAAGCATGCAGAGTTAGTTAGTAAATACGAATATTAC
ATGCATGAGAGAAATAAAATTCTAGCAGAAGACATAAGAGATGATAATTGGCTTAAAATTATTGAAGGAAAGATGGCTGA
TATGTCTAGTCACATTGCTAATAATCGCTTAAAAACCTTAGAATTTATGCAGCAAGCAATAGATGAACTTGAAAATGAGT
TTCCAAAAGCCGATTTATCGATTGACGGCATTGTTGAACAAAGAATATTAGATGGCGAAGAAAATCTTGTTAATTTTATT
ACTGCAGAACTTTATCAAACAAGAAGCAAAGATAAACTACTTGGTCGTACTAGCTTTGGAGTTCATAAAAGCGACTTTTT
AGTAAAGCATCAAAAGAAAAATATCTTAGCGAAATTTTGTTCTACTGGTGAACAAAAAGCTATACTAATTGCAATAATCC
TTGCTGAAATGAATTATGTTATAAAGCTAACTAAAATAGCACCAATTTTACTTTTAGATGAGGTCTTTGTGCATCTAGAC
GATACAAGACGTCAATATTTAATCGAATTTTTTACCACTTTAAGCATGCAACTATGGGTTACAGATACAAATCTAGAGGG
AATAGAAAATTTTGCAAGTAAAGCACAATTGATTAAGTTATAG

Upstream 100 bases:

>100_bases
AATACGTGAACCACAGCTCTTTAAGAACGACATAGCCAATTTTTGAAATTCACCTAGTATACAACGTATCAATAATTTAA
CTATTATTCTCTTTCATAAA

Downstream 100 bases:

>100_bases
GTTATATAATAATGTCATTCCTACTTAGAACAATCCACCGCTTTCGTCCTACGATTTGATCGCGGGATCCAGTTAAAAAT
ACTAATGATTTTAGTATTTT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKNIFLHSLSLANYRNFKNLELKTDNTPIILIGENGSGKTNILEAISLFYPGRGLRSAKLDDICKASEDYCIVKALLQSQ
LGLAEFSTHIKRNSNRRITEYNESKIANNELSKFTSMVWLTPQMEGIFTSGSSDRRKFLDRIVYNFYPKHAELVSKYEYY
MHERNKILAEDIRDDNWLKIIEGKMADMSSHIANNRLKTLEFMQQAIDELENEFPKADLSIDGIVEQRILDGEENLVNFI
TAELYQTRSKDKLLGRTSFGVHKSDFLVKHQKKNILAKFCSTGEQKAILIAIILAEMNYVIKLTKIAPILLLDEVFVHLD
DTRRQYLIEFFTTLSMQLWVTDTNLEGIENFASKAQLIKL

Sequences:

>Translated_360_residues
MKNIFLHSLSLANYRNFKNLELKTDNTPIILIGENGSGKTNILEAISLFYPGRGLRSAKLDDICKASEDYCIVKALLQSQ
LGLAEFSTHIKRNSNRRITEYNESKIANNELSKFTSMVWLTPQMEGIFTSGSSDRRKFLDRIVYNFYPKHAELVSKYEYY
MHERNKILAEDIRDDNWLKIIEGKMADMSSHIANNRLKTLEFMQQAIDELENEFPKADLSIDGIVEQRILDGEENLVNFI
TAELYQTRSKDKLLGRTSFGVHKSDFLVKHQKKNILAKFCSTGEQKAILIAIILAEMNYVIKLTKIAPILLLDEVFVHLD
DTRRQYLIEFFTTLSMQLWVTDTNLEGIENFASKAQLIKL
>Mature_360_residues
MKNIFLHSLSLANYRNFKNLELKTDNTPIILIGENGSGKTNILEAISLFYPGRGLRSAKLDDICKASEDYCIVKALLQSQ
LGLAEFSTHIKRNSNRRITEYNESKIANNELSKFTSMVWLTPQMEGIFTSGSSDRRKFLDRIVYNFYPKHAELVSKYEYY
MHERNKILAEDIRDDNWLKIIEGKMADMSSHIANNRLKTLEFMQQAIDELENEFPKADLSIDGIVEQRILDGEENLVNFI
TAELYQTRSKDKLLGRTSFGVHKSDFLVKHQKKNILAKFCSTGEQKAILIAIILAEMNYVIKLTKIAPILLLDEVFVHLD
DTRRQYLIEFFTTLSMQLWVTDTNLEGIENFASKAQLIKL

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=349, Percent_Identity=23.2091690544413, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 41510; Mature: 41510

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIFLHSLSLANYRNFKNLELKTDNTPIILIGENGSGKTNILEAISLFYPGRGLRSAKL
CCCEEEEHHHHHHCCCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCH
DDICKASEDYCIVKALLQSQLGLAEFSTHIKRNSNRRITEYNESKIANNELSKFTSMVWL
HHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHEEE
TPQMEGIFTSGSSDRRKFLDRIVYNFYPKHAELVSKYEYYMHERNKILAEDIRDDNWLKI
CCCCCCEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEE
IEGKMADMSSHIANNRLKTLEFMQQAIDELENEFPKADLSIDGIVEQRILDGEENLVNFI
ECCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHH
TAELYQTRSKDKLLGRTSFGVHKSDFLVKHQKKNILAKFCSTGEQKAILIAIILAEMNYV
HHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHE
IKLTKIAPILLLDEVFVHLDDTRRQYLIEFFTTLSMQLWVTDTNLEGIENFASKAQLIKL
EEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNIFLHSLSLANYRNFKNLELKTDNTPIILIGENGSGKTNILEAISLFYPGRGLRSAKL
CCCEEEEHHHHHHCCCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCH
DDICKASEDYCIVKALLQSQLGLAEFSTHIKRNSNRRITEYNESKIANNELSKFTSMVWL
HHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHEEE
TPQMEGIFTSGSSDRRKFLDRIVYNFYPKHAELVSKYEYYMHERNKILAEDIRDDNWLKI
CCCCCCEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEE
IEGKMADMSSHIANNRLKTLEFMQQAIDELENEFPKADLSIDGIVEQRILDGEENLVNFI
ECCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHH
TAELYQTRSKDKLLGRTSFGVHKSDFLVKHQKKNILAKFCSTGEQKAILIAIILAEMNYV
HHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHE
IKLTKIAPILLLDEVFVHLDDTRRQYLIEFFTTLSMQLWVTDTNLEGIENFASKAQLIKL
EEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA