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The map label for this gene is tpiA

Identifier: 157373037

GI number: 157373037

Start: 5324170

End: 5324937

Strand: Direct

Name: tpiA

Synonym: Spro_4805

Alternate gene names: 157373037

Gene position: 5324170-5324937 (Clockwise)

Preceding gene: 157373036

Following gene: 157373042

Centisome position: 97.71

GC content: 55.86

Gene sequence:

>768_bases
ATGCGTCATCCATTAGTTATGGGTAACTGGAAGCTTAACGGCAGCACTCACATGGTTAACGAACTGATCGCCGCACTGCG
CAATGAACTGAGCAGTGTTGACGGTTGTGGCGTCGCTATCGCACCACCGGTCATGTACCTGGACCAGGCCAAGCACGCGC
TGGCCGGCAGCCGCATCGCTCTGGGCGCCCAAAACGTAGACGTGAACCTGTCCGGCGCATTCACCGGTGAAGTTTCCGCC
GACATGCTGAAAGATATTGGTGCGCAATACATCATCATCGGCCACTCCGAGCGTCGTACTTACCACAAAGAAACTGACGC
TGCGATCGCCGAGAAATTTGCCGTGCTGAAAACTGCCGGTCTGATCCCAGTGCTGTGCATCGGTGAAACTGACGCTGAAA
ACGAAGCGGGTAAAACCGAAGAAGTTTGCGCACGTCAGATCGACGCCGTACTGAAAACCCAGGGTGCAGAAGCCTTTAAA
GGCGCGGTTATCGCTTATGAGCCAATTTGGGCTATCGGTACCGGCAAGTCTGCCACTCCTGCGCAAGCACAGGCAGTACA
CAAATTTATCCGCGATCACATCGCCAAGCAGGACGCAGCCGTGGCTGCAGAAGTGATCATCCAGTACGGCGGCTCCGTGA
ACGACAAAAATGCTGCCGAGCTGTTCACTCAGCCGGACATCGACGGCGCGCTGGTTGGCGGCGCATCACTGAAAGCCGAT
GCTTTCGCCGTGATCGTCAAAGCCGCTGCTGCTGCTAAAAAAGCCTGA

Upstream 100 bases:

>100_bases
AAGCCTTCGCGCTTAATCATCTCTATACTGTTGGGCAGGTAAACTGCCGGTCCGGCAGTGGTTATTTCGACTCTCGCGTT
CCCTTGTTGGAGGAAAAAAC

Downstream 100 bases:

>100_bases
TTTCTCCCGGCTTATAAATAAAAAACCCCGGCTTGCCGGGGTTTTTTATTGGTGCCCATTGAGCATCAACGTTTGCTGAT
TTCGTCAAACACGCCGCCGG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MRHPLVMGNWKLNGSTHMVNELIAALRNELSSVDGCGVAIAPPVMYLDQAKHALAGSRIALGAQNVDVNLSGAFTGEVSA
DMLKDIGAQYIIIGHSERRTYHKETDAAIAEKFAVLKTAGLIPVLCIGETDAENEAGKTEEVCARQIDAVLKTQGAEAFK
GAVIAYEPIWAIGTGKSATPAQAQAVHKFIRDHIAKQDAAVAAEVIIQYGGSVNDKNAAELFTQPDIDGALVGGASLKAD
AFAVIVKAAAAAKKA

Sequences:

>Translated_255_residues
MRHPLVMGNWKLNGSTHMVNELIAALRNELSSVDGCGVAIAPPVMYLDQAKHALAGSRIALGAQNVDVNLSGAFTGEVSA
DMLKDIGAQYIIIGHSERRTYHKETDAAIAEKFAVLKTAGLIPVLCIGETDAENEAGKTEEVCARQIDAVLKTQGAEAFK
GAVIAYEPIWAIGTGKSATPAQAQAVHKFIRDHIAKQDAAVAAEVIIQYGGSVNDKNAAELFTQPDIDGALVGGASLKAD
AFAVIVKAAAAAKKA
>Mature_255_residues
MRHPLVMGNWKLNGSTHMVNELIAALRNELSSVDGCGVAIAPPVMYLDQAKHALAGSRIALGAQNVDVNLSGAFTGEVSA
DMLKDIGAQYIIIGHSERRTYHKETDAAIAEKFAVLKTAGLIPVLCIGETDAENEAGKTEEVCARQIDAVLKTQGAEAFK
GAVIAYEPIWAIGTGKSATPAQAQAVHKFIRDHIAKQDAAVAAEVIIQYGGSVNDKNAAELFTQPDIDGALVGGASLKAD
AFAVIVKAAAAAKKA

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=245, Percent_Identity=44.4897959183673, Blast_Score=185, Evalue=4e-47,
Organism=Homo sapiens, GI226529917, Length=245, Percent_Identity=44.4897959183673, Blast_Score=185, Evalue=4e-47,
Organism=Escherichia coli, GI1790353, Length=255, Percent_Identity=83.1372549019608, Blast_Score=431, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17536593, Length=247, Percent_Identity=47.7732793522267, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6320255, Length=248, Percent_Identity=46.3709677419355, Blast_Score=199, Evalue=4e-52,
Organism=Drosophila melanogaster, GI28572008, Length=249, Percent_Identity=48.5943775100402, Blast_Score=200, Evalue=6e-52,
Organism=Drosophila melanogaster, GI28572006, Length=249, Percent_Identity=48.5943775100402, Blast_Score=200, Evalue=6e-52,
Organism=Drosophila melanogaster, GI28572004, Length=249, Percent_Identity=48.5943775100402, Blast_Score=199, Evalue=1e-51,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_SERP5 (A8GLA8)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001481026.1
- ProteinModelPortal:   A8GLA8
- SMR:   A8GLA8
- STRING:   A8GLA8
- GeneID:   5605476
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4805
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   SPRO399741:SPRO_4805-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26643; Mature: 26643

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 95-95 ACT_SITE 167-167 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHPLVMGNWKLNGSTHMVNELIAALRNELSSVDGCGVAIAPPVMYLDQAKHALAGSRIA
CCCCEEEECEEECCCHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHCCCEEE
LGAQNVDVNLSGAFTGEVSADMLKDIGAQYIIIGHSERRTYHKETDAAIAEKFAVLKTAG
ECCCCCEEEECCEECCCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
LIPVLCIGETDAENEAGKTEEVCARQIDAVLKTQGAEAFKGAVIAYEPIWAIGTGKSATP
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCEEEECCEEEEECCCCCCC
AQAQAVHKFIRDHIAKQDAAVAAEVIIQYGGSVNDKNAAELFTQPDIDGALVGGASLKAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEEECCCCCCHH
AFAVIVKAAAAAKKA
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MRHPLVMGNWKLNGSTHMVNELIAALRNELSSVDGCGVAIAPPVMYLDQAKHALAGSRIA
CCCCEEEECEEECCCHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHCCCEEE
LGAQNVDVNLSGAFTGEVSADMLKDIGAQYIIIGHSERRTYHKETDAAIAEKFAVLKTAG
ECCCCCEEEECCEECCCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
LIPVLCIGETDAENEAGKTEEVCARQIDAVLKTQGAEAFKGAVIAYEPIWAIGTGKSATP
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCEEEECCEEEEECCCCCCC
AQAQAVHKFIRDHIAKQDAAVAAEVIIQYGGSVNDKNAAELFTQPDIDGALVGGASLKAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEEECCCCCCHH
AFAVIVKAAAAAKKA
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA