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The map label for this gene is dusA [H]

Identifier: 157372686

GI number: 157372686

Start: 4925978

End: 4926988

Strand: Reverse

Name: dusA [H]

Synonym: Spro_4453

Alternate gene names: 157372686

Gene position: 4926988-4925978 (Counterclockwise)

Preceding gene: 157372691

Following gene: 157372685

Centisome position: 90.42

GC content: 57.17

Gene sequence:

>1011_bases
ATGACGACAGACAACAACGCTTCAAAATACGCCGCCAATCGTTTCTCCATCGCGCCGATGCTCGACTGGACCGATCGTCA
TTGCCGTTACTTCCACCGCCTGCTGACCAAGGACACGCTGCTGTACACCGAAATGGTGACTACCGGCGCGATTATCCATG
GCAAGGGCGACTATCTGGCTTACAGCGAAGAAGAACATCCGGTGGCGCTGCAACTGGGCGGTAGCGATCCGGCTGCGTTG
GCACACTGTGCCAAATTGGCTGAGCAGCGCGGCTACGACGAGATTAATCTCAACGTCGGTTGTCCGTCCGATCGGGTGCA
GAATGGCATGTTCGGTGCCTGTCTGATGGGGCAGGCAACGCTGGTTGCCGACTGCATCAAGGCGATGCGTGACGTGGTGT
CGATCCCGGTGACCGTGAAAACCCGTATCGGTATTGACGACCAGGACAGCTATGAGTTTCTGTGTGATTTTATCGCTACC
GTTGCCGGACGCGGTGAATGCGATATGTTCACCATTCATGCGCGCAAAGCCTGGCTGTCGGGCCTGAGCCCGAAAGAGAA
TCGTGAAGTGCCGCCGCTGGATTATCCGCGTGTCTATCAGTTAAAACGTGATTTCCCAGAACTGACTCTTGCCATCAATG
GCGGGGTGAAAACTCTGGAGGAGGCGAAGCAGCACCTACAGCATCTGGACGGCGTAATGATGGGGCGCGAAGCCTATCAG
AACCCAGGCATTCTGGCGCGGGTCGACAGCGAACTGTTTGGCCGCCAGACCGAGGTGATGGACAGCGTAGCGATTATTGA
GTCGCTGTATCCTTACATCGAACGCGAACTGTCGAACGGCACCTATTTGGGCCATATTACCCGCCATATTCTGGGCCTGT
TCCAGGGCGTGCCGGGTGCGCGTCAGTGGCGCCGCCACCTGAGCGAAAATGCCCATAAGCCAGGGGCAGATGCGCGCGTG
GTTGAACAAGCACTGGATTTGGTGCGCCTGCCGCGTACCGAAATGGCGTAA

Upstream 100 bases:

>100_bases
AGGTGGTTTTCCCAAGGCGGCCGATTGTCGTTATAATAGCCGTTTGCGGCAGTACGCCGTTCGTTGAAACCAGACAGAAT
CAGGTAGCCAGTAGCCCAGC

Downstream 100 bases:

>100_bases
TTGACATCAATATCTGACTAAAAGTTAGCGTTTTTGACCAGGCATGTCGCATTGTGTGATGTGCCTTTTTTTTAATTTCA
ATCACTTAGTTTCCACGCCG

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTTDNNASKYAANRFSIAPMLDWTDRHCRYFHRLLTKDTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAAL
AHCAKLAEQRGYDEINLNVGCPSDRVQNGMFGACLMGQATLVADCIKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFIAT
VAGRGECDMFTIHARKAWLSGLSPKENREVPPLDYPRVYQLKRDFPELTLAINGGVKTLEEAKQHLQHLDGVMMGREAYQ
NPGILARVDSELFGRQTEVMDSVAIIESLYPYIERELSNGTYLGHITRHILGLFQGVPGARQWRRHLSENAHKPGADARV
VEQALDLVRLPRTEMA

Sequences:

>Translated_336_residues
MTTDNNASKYAANRFSIAPMLDWTDRHCRYFHRLLTKDTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAAL
AHCAKLAEQRGYDEINLNVGCPSDRVQNGMFGACLMGQATLVADCIKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFIAT
VAGRGECDMFTIHARKAWLSGLSPKENREVPPLDYPRVYQLKRDFPELTLAINGGVKTLEEAKQHLQHLDGVMMGREAYQ
NPGILARVDSELFGRQTEVMDSVAIIESLYPYIERELSNGTYLGHITRHILGLFQGVPGARQWRRHLSENAHKPGADARV
VEQALDLVRLPRTEMA
>Mature_335_residues
TTDNNASKYAANRFSIAPMLDWTDRHCRYFHRLLTKDTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALA
HCAKLAEQRGYDEINLNVGCPSDRVQNGMFGACLMGQATLVADCIKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFIATV
AGRGECDMFTIHARKAWLSGLSPKENREVPPLDYPRVYQLKRDFPELTLAINGGVKTLEEAKQHLQHLDGVMMGREAYQN
PGILARVDSELFGRQTEVMDSVAIIESLYPYIERELSNGTYLGHITRHILGLFQGVPGARQWRRHLSENAHKPGADARVV
EQALDLVRLPRTEMA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=260, Percent_Identity=23.8461538461538, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI145693211, Length=317, Percent_Identity=83.9116719242902, Blast_Score=575, Evalue=1e-165,
Organism=Escherichia coli, GI1789660, Length=245, Percent_Identity=25.3061224489796, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=238, Percent_Identity=25.2100840336134, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24580595, Length=299, Percent_Identity=21.4046822742475, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19920448, Length=299, Percent_Identity=21.4046822742475, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 37550; Mature: 37419

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDNNASKYAANRFSIAPMLDWTDRHCRYFHRLLTKDTLLYTEMVTTGAIIHGKGDYLA
CCCCCCCHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEE
YSEEEHPVALQLGGSDPAALAHCAKLAEQRGYDEINLNVGCPSDRVQNGMFGACLMGQAT
ECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCHHHHHHCCHH
LVADCIKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFIATVAGRGECDMFTIHARKAWLS
HHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHC
GLSPKENREVPPLDYPRVYQLKRDFPELTLAINGGVKTLEEAKQHLQHLDGVMMGREAYQ
CCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHC
NPGILARVDSELFGRQTEVMDSVAIIESLYPYIERELSNGTYLGHITRHILGLFQGVPGA
CCCEEEEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCH
RQWRRHLSENAHKPGADARVVEQALDLVRLPRTEMA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TTDNNASKYAANRFSIAPMLDWTDRHCRYFHRLLTKDTLLYTEMVTTGAIIHGKGDYLA
CCCCCCHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEE
YSEEEHPVALQLGGSDPAALAHCAKLAEQRGYDEINLNVGCPSDRVQNGMFGACLMGQAT
ECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCHHHHHHCCHH
LVADCIKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFIATVAGRGECDMFTIHARKAWLS
HHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHC
GLSPKENREVPPLDYPRVYQLKRDFPELTLAINGGVKTLEEAKQHLQHLDGVMMGREAYQ
CCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHC
NPGILARVDSELFGRQTEVMDSVAIIESLYPYIERELSNGTYLGHITRHILGLFQGVPGA
CCCEEEEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCH
RQWRRHLSENAHKPGADARVVEQALDLVRLPRTEMA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]