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The map label for this gene is pucA [H]

Identifier: 157372254

GI number: 157372254

Start: 4462375

End: 4463427

Strand: Direct

Name: pucA [H]

Synonym: Spro_4020

Alternate gene names: 157372254

Gene position: 4462375-4463427 (Clockwise)

Preceding gene: 157372253

Following gene: 157372257

Centisome position: 81.9

GC content: 59.64

Gene sequence:

>1053_bases
ATGCAACATCTCGATATCACCGTGGTCAACCAGGCGATAAGCTGGCTGAAGCAACAGCCGGTGTGGCTGTGCACCGTACT
GAACACCTACGGCTCCTCACCGCGATCGCCAGGAGCGCTGCTGGTGGCCACCCAGGATGGGCATTACTGTGGTTCGCTGT
CGGGTGGCTGCGTGGAAGAGGATTTTCTGCGGCGCGTAGCGGCCGGGGAATATCAGGCGGCCAGCCAGGTGATCCGTTAT
GGTGAAGGGGGTCTGGAGCCGAATGTAGCACTGCCGTGCGGCGGCGTATTGGAAGTGCTGATAGAATCCCTGCCAGCCGG
TGACGACAGTATTGCCTATCTGACAAAAATTGCCGGTGCACTGAACGGACACCACGCGCTGGTCAAACGCCTGACCTTGC
CCGATGCCTGTACCAGCCTGACATTGACCGATTTCGCCAGCGCCACCCAGGTGGAACGTCGTGGTCCGCAGGTCACTTTG
CATATCGCCGCGGCACCGCGTTTGTTGATCGCCGGGCTGTCCAGCGTAGCGCTGTACTGCGCCGACTTCGCCAGTTCACT
GGGCTTTGAAGTGTTGGTTTGTGAGAATCGGCCGGAGGCGCTGGATAATTTCGCCCCTCAGCTCAGAGGCAACGTCACAT
TGTTGCGCCAGTTCCCGGCCAAGTTTATTGAAGACGGCGGCTGCCACGCCAATACGGCGATTGTGGCGCTGACCCACGAT
CCGCGCATGGACGATTTGACGCTGATGGAAGCGATCCACACCCCGGCATTTTACATCGGTGCCATGGGGTCATTGCGCAA
CAGCGCGCGCCGCCGCCAGCGTCTGCAGCAGATTGCCGAATTCAGCCCACAGGAACTGGAACGTATTCATGCCCCGATCG
GGTTGCCACTCGGCAGTAAAACCCCGGCGGAAATCGCGCTGGCGGTAATGGCGGATATCGTGCAGCAGAAAAACCGCACT
ACCGCGGTCCGATCGGTCAACCAATTGGAGTCACTGGCACCAGATGCTGGAGCTCAAGCTGCAACTCCTCCCGCTCGTTC
AGCGAATAGTTGA

Upstream 100 bases:

>100_bases
CTTAAAGGGCAGCCAGTGTTCCTGCTTTCTCTTGAAACGTCCGAAGTGGTGCAGGATATTGATCTTCCGTCACAGCTACC
GGCCAGCGAGTAAATCATCT

Downstream 100 bases:

>100_bases
ACATCGTCAGGTCTGGCCCTACTCTGCGGGCCAGGCCCAATTCCGGTAGTGCCTGGGTGTAAATCTGCACCACATGGTCG
TGCAAGGCCACCGGCTTGCC

Product: hypothetical protein

Products: NA

Alternate protein names: XDHase subunit A [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MQHLDITVVNQAISWLKQQPVWLCTVLNTYGSSPRSPGALLVATQDGHYCGSLSGGCVEEDFLRRVAAGEYQAASQVIRY
GEGGLEPNVALPCGGVLEVLIESLPAGDDSIAYLTKIAGALNGHHALVKRLTLPDACTSLTLTDFASATQVERRGPQVTL
HIAAAPRLLIAGLSSVALYCADFASSLGFEVLVCENRPEALDNFAPQLRGNVTLLRQFPAKFIEDGGCHANTAIVALTHD
PRMDDLTLMEAIHTPAFYIGAMGSLRNSARRRQRLQQIAEFSPQELERIHAPIGLPLGSKTPAEIALAVMADIVQQKNRT
TAVRSVNQLESLAPDAGAQAATPPARSANS

Sequences:

>Translated_350_residues
MQHLDITVVNQAISWLKQQPVWLCTVLNTYGSSPRSPGALLVATQDGHYCGSLSGGCVEEDFLRRVAAGEYQAASQVIRY
GEGGLEPNVALPCGGVLEVLIESLPAGDDSIAYLTKIAGALNGHHALVKRLTLPDACTSLTLTDFASATQVERRGPQVTL
HIAAAPRLLIAGLSSVALYCADFASSLGFEVLVCENRPEALDNFAPQLRGNVTLLRQFPAKFIEDGGCHANTAIVALTHD
PRMDDLTLMEAIHTPAFYIGAMGSLRNSARRRQRLQQIAEFSPQELERIHAPIGLPLGSKTPAEIALAVMADIVQQKNRT
TAVRSVNQLESLAPDAGAQAATPPARSANS
>Mature_350_residues
MQHLDITVVNQAISWLKQQPVWLCTVLNTYGSSPRSPGALLVATQDGHYCGSLSGGCVEEDFLRRVAAGEYQAASQVIRY
GEGGLEPNVALPCGGVLEVLIESLPAGDDSIAYLTKIAGALNGHHALVKRLTLPDACTSLTLTDFASATQVERRGPQVTL
HIAAAPRLLIAGLSSVALYCADFASSLGFEVLVCENRPEALDNFAPQLRGNVTLLRQFPAKFIEDGGCHANTAIVALTHD
PRMDDLTLMEAIHTPAFYIGAMGSLRNSARRRQRLQQIAEFSPQELERIHAPIGLPLGSKTPAEIALAVMADIVQQKNRT
TAVRSVNQLESLAPDAGAQAATPPARSANS

Specific function: Oxidizes hypoxanthine and xanthine to uric acid. PucA subunit could exert a molybdenum cofactor recruiting function [H]

COG id: COG1975

COG function: function code O; Xanthine and CO dehydrogenases maturation factor, XdhC/CoxF family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786477, Length=291, Percent_Identity=27.1477663230241, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003777 [H]

Pfam domain/function: PF02625 XdhC_CoxI [H]

EC number: =1.17.1.4 [H]

Molecular weight: Translated: 37321; Mature: 37321

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHLDITVVNQAISWLKQQPVWLCTVLNTYGSSPRSPGALLVATQDGHYCGSLSGGCVEE
CCCCCHHHHHHHHHHHHHCCEEEEEEHHHHCCCCCCCCEEEEEECCCCEECCCCCCCCCH
DFLRRVAAGEYQAASQVIRYGEGGLEPNVALPCGGVLEVLIESLPAGDDSIAYLTKIAGA
HHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
LNGHHALVKRLTLPDACTSLTLTDFASATQVERRGPQVTLHIAAAPRLLIAGLSSVALYC
HCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHH
ADFASSLGFEVLVCENRPEALDNFAPQLRGNVTLLRQFPAKFIEDGGCHANTAIVALTHD
HHHHHHCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECC
PRMDDLTLMEAIHTPAFYIGAMGSLRNSARRRQRLQQIAEFSPQELERIHAPIGLPLGSK
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCC
TPAEIALAVMADIVQQKNRTTAVRSVNQLESLAPDAGAQAATPPARSANS
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQHLDITVVNQAISWLKQQPVWLCTVLNTYGSSPRSPGALLVATQDGHYCGSLSGGCVEE
CCCCCHHHHHHHHHHHHHCCEEEEEEHHHHCCCCCCCCEEEEEECCCCEECCCCCCCCCH
DFLRRVAAGEYQAASQVIRYGEGGLEPNVALPCGGVLEVLIESLPAGDDSIAYLTKIAGA
HHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
LNGHHALVKRLTLPDACTSLTLTDFASATQVERRGPQVTLHIAAAPRLLIAGLSSVALYC
HCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHH
ADFASSLGFEVLVCENRPEALDNFAPQLRGNVTLLRQFPAKFIEDGGCHANTAIVALTHD
HHHHHHCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECC
PRMDDLTLMEAIHTPAFYIGAMGSLRNSARRRQRLQQIAEFSPQELERIHAPIGLPLGSK
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCC
TPAEIALAVMADIVQQKNRTTAVRSVNQLESLAPDAGAQAATPPARSANS
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]