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The map label for this gene is ligT [H]

Identifier: 157372217

GI number: 157372217

Start: 4419324

End: 4419866

Strand: Direct

Name: ligT [H]

Synonym: Spro_3983

Alternate gene names: 157372217

Gene position: 4419324-4419866 (Clockwise)

Preceding gene: 157372208

Following gene: 157372218

Centisome position: 81.11

GC content: 62.8

Gene sequence:

>543_bases
ATGTCCAGCTCGCGCCGCCTGTTTTTCGCCCTGACCTTGCCTGATGCGCTGCAGCAACAGGTGATCCGCTGGCGCGCCGA
AGCCTTCGCGCCGGAAGTCGGCCGGCCGGTCGCGGCAGCCAATTTACATCTGACGCTGGCCTTTCTTGGTGACGTTTCGG
CGCAAAAAGAATCCGCATTGAAAAAGCTGGCCGCCCGTATCAGCCAGCCCGGTTTCAGCATGGTGCTCGATGACCTCGGC
CACTGGCCGCGCCCCGGCGTGGTCTGGCTCGGCACCCGGCGGGCACCGCGTGGGCTATTGCAGTTGGCGGAGCTGTTGCG
ATCGCAAGCGGCCCGCAGCGGCTGCTATCAAAACCCCATGCCGTTTCATCCGCATATCACCCTGCTGCGCAGCGCAACGC
AGCCGGTCGCCGTACCGCCCGCTACGCCGGGCTGGAACTTCAGCGCTAACGAATTCGCCCTGTACCAGTCAGTGTTTGAA
AACGGCCGCACGCGTTATCAGATGCTTGAGCAATGGTCTTTAGCCCATCAGGATACGCCATGA

Upstream 100 bases:

>100_bases
GCTGACGGGCAGTGAAGACACAGAACTCTCCGTAGTGGTTAACATTGGACGGCGCACATTGTAGCATTAGACTCCGCCTT
TCATTACTTGCCGGGAACCT

Downstream 100 bases:

>100_bases
TTTTTTCGCCACCGTTGCACCACGCCACGCTGATTAAACGTTACAAACGCTTTCTGGCGGACGTCATTACCCCGGAAGGG
GAAACCTTCACCCTGCACTG

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MSSSRRLFFALTLPDALQQQVIRWRAEAFAPEVGRPVAAANLHLTLAFLGDVSAQKESALKKLAARISQPGFSMVLDDLG
HWPRPGVVWLGTRRAPRGLLQLAELLRSQAARSGCYQNPMPFHPHITLLRSATQPVAVPPATPGWNFSANEFALYQSVFE
NGRTRYQMLEQWSLAHQDTP

Sequences:

>Translated_180_residues
MSSSRRLFFALTLPDALQQQVIRWRAEAFAPEVGRPVAAANLHLTLAFLGDVSAQKESALKKLAARISQPGFSMVLDDLG
HWPRPGVVWLGTRRAPRGLLQLAELLRSQAARSGCYQNPMPFHPHITLLRSATQPVAVPPATPGWNFSANEFALYQSVFE
NGRTRYQMLEQWSLAHQDTP
>Mature_179_residues
SSSRRLFFALTLPDALQQQVIRWRAEAFAPEVGRPVAAANLHLTLAFLGDVSAQKESALKKLAARISQPGFSMVLDDLGH
WPRPGVVWLGTRRAPRGLLQLAELLRSQAARSGCYQNPMPFHPHITLLRSATQPVAVPPATPGWNFSANEFALYQSVFEN
GRTRYQMLEQWSLAHQDTP

Specific function: Specifically ligates tRNA half-molecules containing nucleoside base modifications and shows a preference among different tRNA species. Acts in the absence of ATP to form a 2'-5' phosphodiester linkage [H]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081692, Length=176, Percent_Identity=60.7954545454545, Blast_Score=224, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 20056; Mature: 19925

Theoretical pI: Translated: 10.72; Mature: 10.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSRRLFFALTLPDALQQQVIRWRAEAFAPEVGRPVAAANLHLTLAFLGDVSAQKESAL
CCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEHHHHEEEEHHHHCCCHHHHHHH
KKLAARISQPGFSMVLDDLGHWPRPGVVWLGTRRAPRGLLQLAELLRSQAARSGCYQNPM
HHHHHHHCCCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCC
PFHPHITLLRSATQPVAVPPATPGWNFSANEFALYQSVFENGRTRYQMLEQWSLAHQDTP
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SSSRRLFFALTLPDALQQQVIRWRAEAFAPEVGRPVAAANLHLTLAFLGDVSAQKESAL
CCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEHHHHEEEEHHHHCCCHHHHHHH
KKLAARISQPGFSMVLDDLGHWPRPGVVWLGTRRAPRGLLQLAELLRSQAARSGCYQNPM
HHHHHHHCCCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCC
PFHPHITLLRSATQPVAVPPATPGWNFSANEFALYQSVFENGRTRYQMLEQWSLAHQDTP
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 2013578; 8940112 [H]