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The map label for this gene is mrcB [H]

Identifier: 157372215

GI number: 157372215

Start: 4414196

End: 4416706

Strand: Reverse

Name: mrcB [H]

Synonym: Spro_3981

Alternate gene names: 157372215

Gene position: 4416706-4414196 (Counterclockwise)

Preceding gene: 157372216

Following gene: 157372214

Centisome position: 81.06

GC content: 58.5

Gene sequence:

>2511_bases
ATGGCTGGGGATGACCGCGAGCCCATCGGGCGCAAAGGGAAACAACCAAAACGCACCGCGTCGCGCAAACCGTCGCGCCG
TCGTCGAGATGACGATTACGATGATTACGAGGAAGACGAGTACCAGGATGACGAAGATGATTATGAAGACGAGGAAGAAC
GCATGCCGCGTAAGGTAAAAGCGACTCCGCCGCGTAAAAAACGCCGCTGGCTCGGCTTGCTGATCAAACTGATTCTGGTG
GTTGCCGTGCTGTTGGCAATCTACGGCGTTTATCTGGATTCGCAGATCCGCAGCCGCATTGACGGCAAGGTCTGGCAGTT
ACCGGCGGCGGTGTATGGCCGCATGGTCAACCTGGAACCGGGTATGCCGTACAGCAAAAAGGAAATGGTCGATCTGCTGC
AGGGCATGCAATACCGCCAGGTCAGCCGCATGACCCGTCCGGGCGAATTTACCGTACAGGCCAACAGCATTGAGATGCTG
CGTCGTCCATTTGACTTCCCGGACGGTAAAGAAGGGCAGATCCACGCCCGTCTGGATTTCCAGAACAACCGCCTGGCGAA
AATCGAGAACATGGAAAACCAGCGCAGTTTCGGGTTCTTCCGCCTCGATCCGCGCCTGATCACCATGTTGCAGTCACCTA
ACGGTGAACAACGTCTGTTTGTCCCGCGCGCCGGTTTCCCGGACCTGCTGGTGGACACGTTGATCGCCACCGAAGACCGT
CACTTCTATGAACATGACGGCATCAGCCCATACTCCATCGGCCGTGCCGTGCTGGCTAACCTGACCGCCGGGCGTGCGGT
GCAGGGCGGCAGTACCCTGACGCAGCAGCTGGTGAAAAACCTGTTCCTGACCAACGAGCGTTCGCTGTGGCGTAAGGCCA
ACGAAGCCTATATGGCGTTGCTGGTGGATTACCGTTACAGCAAGGATCGTATCCTTGAGCTGTATCTGAACGAGGTTTAC
CTCGGCCAGAGCGGCAACGACCAGATCCGCGGTTTCCCGCTGGCCAGCCTGTATTACTTCGGTCGCCCGGTGGATGAACT
GAGTCTGGATCAGCAGGCGCTGCTGGTTGGCATGGTTAAAGGGGCGTCGCTGTATAACCCTTGGCGTAACCCTAAACTGG
CGCTGGAACGTCGTAACCTGGTACTGAAACTGTTGCAGAACCAGGGGGTTATCGATGCCGAACTGTATAACATGCTCAGC
GCCAGGCCGTTGGGCGTGCAGCCGAAAGGTGGGGTGATCACACCACAGCCGGCGTTTATGCAGATGGTGCGTCAGGAACT
GCAAGCCAAGTTGGGCGACAAGGTTAACGATCTGTCCGGTGTGAAAATCTTCACCACGCTGGATCCGGTTTCGCAGGATG
CGGCGGAAAAAGCGGTCGAAGACGGTATTCCGGCATTGAGGGCGGCTCGTCACGTGCAGGATCTGGAAGCGGCGATGGTG
ATTGTCGATCGTTTCAGTGGTGAAGTCCGCGCCATGGTCGGCGGTGCCAACCCGCAGTTCGCCGGGTTTAATCGTGCGAT
GCAGGCGCGCCGTTTGGTCGGTTCACTGGCCAAGCCACCCACTTACCTGACCGCGCTTTCCGAGCCGGATAAATACCGTT
TGAATACCTGGCTGGCCGATCAGCCGTTGACGCTGAAACTGCAGAACGGCACCGTCTGGCAGCCGAACAACTATGACCGT
AAGTTCCGTGGGCAGGTGATGTTGGTGGATGGCCTTGCCTTCTCACTCAACGTGCCGACGGTGAACCTGGGTATGGCGGT
TGGGCTGGATCAAATCAGTGCCACGTTGCAACGTCTGGGCATTCCGAAAGCGGAAATCAATCCGGTACCTTCCATGCTGC
TCGGTGCGATTGGCCTGACGCCGATGGAAGTGGCGCAGGAATACCAGACCATCGCCGGTGGCGGTAACCGTGCGACGCTG
TCTGCGGTGCGCTCGGTGATCGCCGAAGACGGTTCGGTGCTGTACCAGAGCTTCCCGCAGGCGGAACGCGTGGTACCGGC
ACAGGCCGCTTATCTGACGCTGTACGCCATGCAACAGGGTGTCGCACGCGGGACTTCTCGCTCGTTGTCGGTGAAATTCC
CCAACTACAATCTGGCGGCGAAAACCGGTACCACCAACGATCTGCGCGACAGCTGGTTTGCCGGTATTGATGGCAAGGAA
GTGGCTATCGCCTGGGTGGGGCGTGATAACAACGGCCCGGCCAAACTGACCGGTGCCAACGGTGCGCTGACCCTGTATCG
CCGCTATCTGGAAAACCAGACGCCGCTGCCGCTGATGCTGCAGCCGCCGGAAGGTATCAACCAGATGGGCATCGATTCAG
GCGGTAACTTTGTCTGTGGCGGCGGCGGGCGCACCATTCCGGTGTGGACCGATAATCCGCAAGGATTGTGTCAGGCAAGC
CAGGCAGCTCAGCAACAGCAGCAACAGCAACAGCAACAGCCGCAGGATGCGGGTGAGCAAAAAGATGCTGACGGCGTTGC
CGGTTGGATCAAGGACATGTTCGGCAAGTAA

Upstream 100 bases:

>100_bases
AATGAATTCAGATGTTTCTTCTGTTCAGCGGCGTCACGCCACGACAGATTAAGCGACTTATAATGGCCGGCAGTGCCGCC
AAGTCTGGAGATTAACAGCA

Downstream 100 bases:

>100_bases
ACATTGCTGAAACCTTCCCTAAAGGGCACAAATTTTTGTGCCCTTTTTCATGTTTATATCAATATGTTGGCATTTTCTAT
TCCCTATTATCGGTGTCGAT

Product: penicillin-binding protein 1b

Products: NA

Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 836; Mature: 835

Protein sequence:

>836_residues
MAGDDREPIGRKGKQPKRTASRKPSRRRRDDDYDDYEEDEYQDDEDDYEDEEERMPRKVKATPPRKKRRWLGLLIKLILV
VAVLLAIYGVYLDSQIRSRIDGKVWQLPAAVYGRMVNLEPGMPYSKKEMVDLLQGMQYRQVSRMTRPGEFTVQANSIEML
RRPFDFPDGKEGQIHARLDFQNNRLAKIENMENQRSFGFFRLDPRLITMLQSPNGEQRLFVPRAGFPDLLVDTLIATEDR
HFYEHDGISPYSIGRAVLANLTAGRAVQGGSTLTQQLVKNLFLTNERSLWRKANEAYMALLVDYRYSKDRILELYLNEVY
LGQSGNDQIRGFPLASLYYFGRPVDELSLDQQALLVGMVKGASLYNPWRNPKLALERRNLVLKLLQNQGVIDAELYNMLS
ARPLGVQPKGGVITPQPAFMQMVRQELQAKLGDKVNDLSGVKIFTTLDPVSQDAAEKAVEDGIPALRAARHVQDLEAAMV
IVDRFSGEVRAMVGGANPQFAGFNRAMQARRLVGSLAKPPTYLTALSEPDKYRLNTWLADQPLTLKLQNGTVWQPNNYDR
KFRGQVMLVDGLAFSLNVPTVNLGMAVGLDQISATLQRLGIPKAEINPVPSMLLGAIGLTPMEVAQEYQTIAGGGNRATL
SAVRSVIAEDGSVLYQSFPQAERVVPAQAAYLTLYAMQQGVARGTSRSLSVKFPNYNLAAKTGTTNDLRDSWFAGIDGKE
VAIAWVGRDNNGPAKLTGANGALTLYRRYLENQTPLPLMLQPPEGINQMGIDSGGNFVCGGGGRTIPVWTDNPQGLCQAS
QAAQQQQQQQQQQPQDAGEQKDADGVAGWIKDMFGK

Sequences:

>Translated_836_residues
MAGDDREPIGRKGKQPKRTASRKPSRRRRDDDYDDYEEDEYQDDEDDYEDEEERMPRKVKATPPRKKRRWLGLLIKLILV
VAVLLAIYGVYLDSQIRSRIDGKVWQLPAAVYGRMVNLEPGMPYSKKEMVDLLQGMQYRQVSRMTRPGEFTVQANSIEML
RRPFDFPDGKEGQIHARLDFQNNRLAKIENMENQRSFGFFRLDPRLITMLQSPNGEQRLFVPRAGFPDLLVDTLIATEDR
HFYEHDGISPYSIGRAVLANLTAGRAVQGGSTLTQQLVKNLFLTNERSLWRKANEAYMALLVDYRYSKDRILELYLNEVY
LGQSGNDQIRGFPLASLYYFGRPVDELSLDQQALLVGMVKGASLYNPWRNPKLALERRNLVLKLLQNQGVIDAELYNMLS
ARPLGVQPKGGVITPQPAFMQMVRQELQAKLGDKVNDLSGVKIFTTLDPVSQDAAEKAVEDGIPALRAARHVQDLEAAMV
IVDRFSGEVRAMVGGANPQFAGFNRAMQARRLVGSLAKPPTYLTALSEPDKYRLNTWLADQPLTLKLQNGTVWQPNNYDR
KFRGQVMLVDGLAFSLNVPTVNLGMAVGLDQISATLQRLGIPKAEINPVPSMLLGAIGLTPMEVAQEYQTIAGGGNRATL
SAVRSVIAEDGSVLYQSFPQAERVVPAQAAYLTLYAMQQGVARGTSRSLSVKFPNYNLAAKTGTTNDLRDSWFAGIDGKE
VAIAWVGRDNNGPAKLTGANGALTLYRRYLENQTPLPLMLQPPEGINQMGIDSGGNFVCGGGGRTIPVWTDNPQGLCQAS
QAAQQQQQQQQQQPQDAGEQKDADGVAGWIKDMFGK
>Mature_835_residues
AGDDREPIGRKGKQPKRTASRKPSRRRRDDDYDDYEEDEYQDDEDDYEDEEERMPRKVKATPPRKKRRWLGLLIKLILVV
AVLLAIYGVYLDSQIRSRIDGKVWQLPAAVYGRMVNLEPGMPYSKKEMVDLLQGMQYRQVSRMTRPGEFTVQANSIEMLR
RPFDFPDGKEGQIHARLDFQNNRLAKIENMENQRSFGFFRLDPRLITMLQSPNGEQRLFVPRAGFPDLLVDTLIATEDRH
FYEHDGISPYSIGRAVLANLTAGRAVQGGSTLTQQLVKNLFLTNERSLWRKANEAYMALLVDYRYSKDRILELYLNEVYL
GQSGNDQIRGFPLASLYYFGRPVDELSLDQQALLVGMVKGASLYNPWRNPKLALERRNLVLKLLQNQGVIDAELYNMLSA
RPLGVQPKGGVITPQPAFMQMVRQELQAKLGDKVNDLSGVKIFTTLDPVSQDAAEKAVEDGIPALRAARHVQDLEAAMVI
VDRFSGEVRAMVGGANPQFAGFNRAMQARRLVGSLAKPPTYLTALSEPDKYRLNTWLADQPLTLKLQNGTVWQPNNYDRK
FRGQVMLVDGLAFSLNVPTVNLGMAVGLDQISATLQRLGIPKAEINPVPSMLLGAIGLTPMEVAQEYQTIAGGGNRATLS
AVRSVIAEDGSVLYQSFPQAERVVPAQAAYLTLYAMQQGVARGTSRSLSVKFPNYNLAAKTGTTNDLRDSWFAGIDGKEV
AIAWVGRDNNGPAKLTGANGALTLYRRYLENQTPLPLMLQPPEGINQMGIDSGGNFVCGGGGRTIPVWTDNPQGLCQASQ
AAQQQQQQQQQQPQDAGEQKDADGVAGWIKDMFGK

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=851, Percent_Identity=71.7978848413631, Blast_Score=1170, Evalue=0.0,
Organism=Escherichia coli, GI87082258, Length=281, Percent_Identity=34.8754448398576, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1788867, Length=513, Percent_Identity=29.6296296296296, Blast_Score=153, Evalue=5e-38,
Organism=Escherichia coli, GI1789601, Length=127, Percent_Identity=37.7952755905512, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011813
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: =2.4.1.129 [H]

Molecular weight: Translated: 93177; Mature: 93045

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGDDREPIGRKGKQPKRTASRKPSRRRRDDDYDDYEEDEYQDDEDDYEDEEERMPRKVK
CCCCCCCCCCCCCCCCCHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCC
ATPPRKKRRWLGLLIKLILVVAVLLAIYGVYLDSQIRSRIDGKVWQLPAAVYGRMVNLEP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECHHHHCEEEECCC
GMPYSKKEMVDLLQGMQYRQVSRMTRPGEFTVQANSIEMLRRPFDFPDGKEGQIHARLDF
CCCCCHHHHHHHHHCCHHHHHHHCCCCCCEEEECCHHHHHHCCCCCCCCCCCEEEEEEEC
QNNRLAKIENMENQRSFGFFRLDPRLITMLQSPNGEQRLFVPRAGFPDLLVDTLIATEDR
CCCCEEEHHCCCCCCCCCEEEECHHHHHEECCCCCCCEEEEECCCCHHHHHHHHHHCCCC
HFYEHDGISPYSIGRAVLANLTAGRAVQGGSTLTQQLVKNLFLTNERSLWRKANEAYMAL
CHHHCCCCCHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHEEE
LVDYRYSKDRILELYLNEVYLGQSGNDQIRGFPLASLYYFGRPVDELSLDQQALLVGMVK
EEECCCCHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHCCCHHHHCCCHHHHHHHHHC
GASLYNPWRNPKLALERRNLVLKLLQNQGVIDAELYNMLSARPLGVQPKGGVITPQPAFM
CCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
QMVRQELQAKLGDKVNDLSGVKIFTTLDPVSQDAAEKAVEDGIPALRAARHVQDLEAAMV
HHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
IVDRFSGEVRAMVGGANPQFAGFNRAMQARRLVGSLAKPPTYLTALSEPDKYRLNTWLAD
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEECC
QPLTLKLQNGTVWQPNNYDRKFRGQVMLVDGLAFSLNVPTVNLGMAVGLDQISATLQRLG
CCEEEEECCCEEECCCCCCHHHCCCEEEEECEEEEEECCEEECHHHHHHHHHHHHHHHHC
IPKAEINPVPSMLLGAIGLTPMEVAQEYQTIAGGGNRATLSAVRSVIAEDGSVLYQSFPQ
CCHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHCCCH
AERVVPAQAAYLTLYAMQQGVARGTSRSLSVKFPNYNLAAKTGTTNDLRDSWFAGIDGKE
HHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCHHHHHHHCCCCCCE
VAIAWVGRDNNGPAKLTGANGALTLYRRYLENQTPLPLMLQPPEGINQMGIDSGGNFVCG
EEEEEEECCCCCCEEEECCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCEEEC
GGGRTIPVWTDNPQGLCQASQAAQQQQQQQQQQPQDAGEQKDADGVAGWIKDMFGK
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AGDDREPIGRKGKQPKRTASRKPSRRRRDDDYDDYEEDEYQDDEDDYEDEEERMPRKVK
CCCCCCCCCCCCCCCCHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCC
ATPPRKKRRWLGLLIKLILVVAVLLAIYGVYLDSQIRSRIDGKVWQLPAAVYGRMVNLEP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECHHHHCEEEECCC
GMPYSKKEMVDLLQGMQYRQVSRMTRPGEFTVQANSIEMLRRPFDFPDGKEGQIHARLDF
CCCCCHHHHHHHHHCCHHHHHHHCCCCCCEEEECCHHHHHHCCCCCCCCCCCEEEEEEEC
QNNRLAKIENMENQRSFGFFRLDPRLITMLQSPNGEQRLFVPRAGFPDLLVDTLIATEDR
CCCCEEEHHCCCCCCCCCEEEECHHHHHEECCCCCCCEEEEECCCCHHHHHHHHHHCCCC
HFYEHDGISPYSIGRAVLANLTAGRAVQGGSTLTQQLVKNLFLTNERSLWRKANEAYMAL
CHHHCCCCCHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHEEE
LVDYRYSKDRILELYLNEVYLGQSGNDQIRGFPLASLYYFGRPVDELSLDQQALLVGMVK
EEECCCCHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHCCCHHHHCCCHHHHHHHHHC
GASLYNPWRNPKLALERRNLVLKLLQNQGVIDAELYNMLSARPLGVQPKGGVITPQPAFM
CCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
QMVRQELQAKLGDKVNDLSGVKIFTTLDPVSQDAAEKAVEDGIPALRAARHVQDLEAAMV
HHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
IVDRFSGEVRAMVGGANPQFAGFNRAMQARRLVGSLAKPPTYLTALSEPDKYRLNTWLAD
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEECC
QPLTLKLQNGTVWQPNNYDRKFRGQVMLVDGLAFSLNVPTVNLGMAVGLDQISATLQRLG
CCEEEEECCCEEECCCCCCHHHCCCEEEEECEEEEEECCEEECHHHHHHHHHHHHHHHHC
IPKAEINPVPSMLLGAIGLTPMEVAQEYQTIAGGGNRATLSAVRSVIAEDGSVLYQSFPQ
CCHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHCCCH
AERVVPAQAAYLTLYAMQQGVARGTSRSLSVKFPNYNLAAKTGTTNDLRDSWFAGIDGKE
HHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCHHHHHHHCCCCCCE
VAIAWVGRDNNGPAKLTGANGALTLYRRYLENQTPLPLMLQPPEGINQMGIDSGGNFVCG
EEEEEEECCCCCCEEEECCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCEEEC
GGGRTIPVWTDNPQGLCQASQAAQQQQQQQQQQPQDAGEQKDADGVAGWIKDMFGK
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3882429; 8202364; 9278503; 3920658; 8645198; 9244263; 3330753; 1885547; 10037771; 10564478; 9841666 [H]