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The map label for this gene is rluF [H]

Identifier: 157372205

GI number: 157372205

Start: 4399088

End: 4399948

Strand: Reverse

Name: rluF [H]

Synonym: Spro_3971

Alternate gene names: 157372205

Gene position: 4399948-4399088 (Counterclockwise)

Preceding gene: 157372206

Following gene: 157372203

Centisome position: 80.75

GC content: 54.7

Gene sequence:

>861_bases
ATGCTGACCAAACCTTCCACACGTCTCAACAAATATATAAGCGAGAGCGGTATCTGCTCGCGCCGCGATGCCGATCGTTA
CATCGAACAAGGCAATGTTTTTATCAATGGCAAGCGCGTGACGCTTGGCGATCAGGTGTTCCCAGGCGATGTCGTCAAGG
TCAACGGTCAGCTGATTGAACCGCGTAATGAAGACAATCTGATCTTTATCGCGTTGAACAAGCCAGTCGGTATTGTCAGC
ACCACGGAAGAAGGCGAGAAAGACAATATTGTCGATTTCGTCAATCACAGCACCCGTATCTTCCCGATCGGGCGATTGGA
TAAAGATTCACAGGGCCTGATCTTCCTCACCAACCACGGCGATCTGGTGAACAAGATCCTGCGGGCGGGCAACGACCACG
AGAAAGAGTATTTGGTCACGGTCAACAAACCGGTAACCGATGACTTCATTCGTGGCCTGGGGGCCGGCGTGCCAATGCTG
GGTACGGTGACCAAAAAGTGCAAGGTGAAGAAAGAAGCGCCATTTGTGTTTCGCATCGTGCTGGTGCAGGGGCTTAACCG
CCAGATCCGCCGTATGTGCGAGCATTTCGGCTATGAGGTGACCAAGCTGGAGCGTACCCGCATTATGAATGTGGGCATGG
CCGGGTTGCCTCCGGGTGAATGGCGCGACCTAACCGACGATGAGCTGGTCGAGCTGTTCAAATCGATCGAAGGCTCTTCC
TCGGAAGCCAAGCCGGCGAAGAAAGCCAAACCGGCGGCGAAAAAGCCCGCCAGTGCGCCGAAAAGCGTCGAAAAGCCAGC
CGGAGGTGCTCCTGCGCGTAAGCGCTTCGCTCAGCCTGGCCGTAAAAAGAAAGGGCGTTAA

Upstream 100 bases:

>100_bases
CAGCTCGCTCATCCCCAGGAGCTTACTTTTGTAAGTGACTGGGGTGGGTGAGTGCAGGTAACAACGCTGTGGCTTGAAAG
ACGACGGGTATGGAGAAAAT

Downstream 100 bases:

>100_bases
GCCGCAGTAACATGAATATGAGTCGGGGCGCGTTATCTGCCCCGACCCTTTTACGCCCGCCTTGCCTCACGCGTCATCAG
CATCCGGCTGAACAGCACGC

Product: 23S rRNA pseudouridine synthase F

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MLTKPSTRLNKYISESGICSRRDADRYIEQGNVFINGKRVTLGDQVFPGDVVKVNGQLIEPRNEDNLIFIALNKPVGIVS
TTEEGEKDNIVDFVNHSTRIFPIGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVNKPVTDDFIRGLGAGVPML
GTVTKKCKVKKEAPFVFRIVLVQGLNRQIRRMCEHFGYEVTKLERTRIMNVGMAGLPPGEWRDLTDDELVELFKSIEGSS
SEAKPAKKAKPAAKKPASAPKSVEKPAGGAPARKRFAQPGRKKKGR

Sequences:

>Translated_286_residues
MLTKPSTRLNKYISESGICSRRDADRYIEQGNVFINGKRVTLGDQVFPGDVVKVNGQLIEPRNEDNLIFIALNKPVGIVS
TTEEGEKDNIVDFVNHSTRIFPIGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVNKPVTDDFIRGLGAGVPML
GTVTKKCKVKKEAPFVFRIVLVQGLNRQIRRMCEHFGYEVTKLERTRIMNVGMAGLPPGEWRDLTDDELVELFKSIEGSS
SEAKPAKKAKPAAKKPASAPKSVEKPAGGAPARKRFAQPGRKKKGR
>Mature_286_residues
MLTKPSTRLNKYISESGICSRRDADRYIEQGNVFINGKRVTLGDQVFPGDVVKVNGQLIEPRNEDNLIFIALNKPVGIVS
TTEEGEKDNIVDFVNHSTRIFPIGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVNKPVTDDFIRGLGAGVPML
GTVTKKCKVKKEAPFVFRIVLVQGLNRQIRRMCEHFGYEVTKLERTRIMNVGMAGLPPGEWRDLTDDELVELFKSIEGSS
SEAKPAKKAKPAAKKPASAPKSVEKPAGGAPARKRFAQPGRKKKGR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=296, Percent_Identity=76.3513513513514, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1787524, Length=282, Percent_Identity=30.8510638297872, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1788510, Length=231, Percent_Identity=29.4372294372294, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI87081838, Length=187, Percent_Identity=29.4117647058824, Blast_Score=78, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 31720; Mature: 31720

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKPSTRLNKYISESGICSRRDADRYIEQGNVFINGKRVTLGDQVFPGDVVKVNGQLIE
CCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCEEECCEEEEECCCCCCCCEEEECCEEEC
PRNEDNLIFIALNKPVGIVSTTEEGEKDNIVDFVNHSTRIFPIGRLDKDSQGLIFLTNHG
CCCCCCEEEEEECCCCEEEECCCCCCCCCHHHHHCCCEEEEECCCCCCCCCCEEEEECCC
DLVNKILRAGNDHEKEYLVTVNKPVTDDFIRGLGAGVPMLGTVTKKCKVKKEAPFVFRIV
HHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHH
LVQGLNRQIRRMCEHFGYEVTKLERTRIMNVGMAGLPPGEWRDLTDDELVELFKSIEGSS
HHHCCCHHHHHHHHHHCCEEEHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
SEAKPAKKAKPAAKKPASAPKSVEKPAGGAPARKRFAQPGRKKKGR
CCCCCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLTKPSTRLNKYISESGICSRRDADRYIEQGNVFINGKRVTLGDQVFPGDVVKVNGQLIE
CCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCEEECCEEEEECCCCCCCCEEEECCEEEC
PRNEDNLIFIALNKPVGIVSTTEEGEKDNIVDFVNHSTRIFPIGRLDKDSQGLIFLTNHG
CCCCCCEEEEEECCCCEEEECCCCCCCCCHHHHHCCCEEEEECCCCCCCCCCEEEEECCC
DLVNKILRAGNDHEKEYLVTVNKPVTDDFIRGLGAGVPMLGTVTKKCKVKKEAPFVFRIV
HHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHH
LVQGLNRQIRRMCEHFGYEVTKLERTRIMNVGMAGLPPGEWRDLTDDELVELFKSIEGSS
HHHCCCHHHHHHHHHHCCEEEHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
SEAKPAKKAKPAAKKPASAPKSVEKPAGGAPARKRFAQPGRKKKGR
CCCCCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]