Click here to switch to the map view.

The map label for this gene is pcaK [H]

Identifier: 157372175

GI number: 157372175

Start: 4365279

End: 4366628

Strand: Direct

Name: pcaK [H]

Synonym: Spro_3941

Alternate gene names: 157372175

Gene position: 4365279-4366628 (Clockwise)

Preceding gene: 157372174

Following gene: 157372177

Centisome position: 80.11

GC content: 61.78

Gene sequence:

>1350_bases
ATGAGCCAAAACACCGCTCGTATCAGTGAGATTATCGACAACGCCAAGGTCTCGCCTTACCAGATCCTGATCCTCAGCCT
GTGTTTTCTGATCGTCCTGCTGGACGGTTTTGATACCGCCATCATTGGCTATATCGCCCCCGCGCTGCGCGAAGAGTGGG
ATCTGCTGCCTGCGCAGTTATCCCCCGCCTTTGGTGCCGGGTTGTTCGGTCTGCTGCTCGGCAGTCTGATCTTCGGTCCG
GTCGCCGATGCCATTGGCCGCAAACGGGTGCTGCTGGTTTCGATACTGATTTTTGGCGGGGGGACTTTGGCCTCGGCCTA
TACCCATTCCATTGAGTCGCTGACCCTGCTGCGGTTAATTACCGGCATTGGTTTGGGGGGCGCGATGCCCACCTGCATCA
CCCTCAGTTCAGAGTACTCCCCGGCACGTCGGCGGATGATCATGGTCACCCTGAGTTGGAGCGGTTTCACCGCCGGTCTG
GCCCTGGGTGGCCTGCTGGCCGGGCAGATTATTCCGGTGTTCGGCTGGCGTGGCGTGCTGATGTTGGGTGGCATAGCGCC
GTTAATGCTGCTGCCGTTGCTGGCCTGGCAGATGCCCGAGTCAGTGCGCTTTATGGCCTCCAGCCCGAAACATGCCGCGG
GCCTGCGCCAGGTGGTTGAGCGTATCACTGGCAAAAGCTGGGCTGGCGTAACGATCCTCGATGACGAGCGCCCAACGCAG
GCGCGGTCGCCGATGACCCATCTGTTCACCGAAGGCCGCGCAGTGCGGACCCTGCTGCTGTGGGCCGCGTTCTTCTGCTC
GCTGTTCGTGTTTTACCAGCTCACCAGTTGGCTGCCGAGCATTCTCAAGGACGCCGGTTACGATATCGTTCACGCATCAC
GCATTGGTGCGATGCTGCCACTGGGCGGGACTCTCGGGGCTATCCTGATGGCGCTGCTGATGGACCGCGTCGGGCCTTAC
CCGGTTCTGGCCCTGTCCTACCTGGGTGCCGCGCTGGTTGTCGGTGCTACCGGCTATCTGATGGGGGATGTCTACACGCT
GGCCGCCACCGTGTTCCTTGTCGGCTTCGGTGTGGCAGGCGCACAGAACGGTCTGAATCTGGTTTCCGCCACTATCTACC
CCACTGCGGCTCGCGTCACCGGGGTAAGCTGGGCGATGGCGAGTGGCCGCTTCGGCTCCATTGTCGGCTCCATGCTCGGT
GCCTGGATGATTGCCGCTGCAGGTTCCCCGGAGACGTTCTTTATCTGGCTGGCGCTACCGGTGTTGGTGGGTTCTGCCGC
CATCTTCCTGCTCTACCGGCTCAGTTTGAGCCGCCGGGCATCTGCGCATGACCCTGCGTTGCCCAACTGA

Upstream 100 bases:

>100_bases
CCAGCCAAAGCGGCTTTGGCTCAGGCCCGTTTCAGCCGGAACCTGACGCCTATTCTGCCCTCCAGCCAGCTCCACACCAG
CCCAATTTAGTGGAACTACC

Downstream 100 bases:

>100_bases
ACAAATAAAAAAGGGGCCAAGGCCCCTTTCCGTTATTCCGCTTTCGCGGTGGTCGCATCGTTGCGATACAGATGAACGTC
CATCTGCGGGAACGGAATAC

Product: major facilitator transporter

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSQNTARISEIIDNAKVSPYQILILSLCFLIVLLDGFDTAIIGYIAPALREEWDLLPAQLSPAFGAGLFGLLLGSLIFGP
VADAIGRKRVLLVSILIFGGGTLASAYTHSIESLTLLRLITGIGLGGAMPTCITLSSEYSPARRRMIMVTLSWSGFTAGL
ALGGLLAGQIIPVFGWRGVLMLGGIAPLMLLPLLAWQMPESVRFMASSPKHAAGLRQVVERITGKSWAGVTILDDERPTQ
ARSPMTHLFTEGRAVRTLLLWAAFFCSLFVFYQLTSWLPSILKDAGYDIVHASRIGAMLPLGGTLGAILMALLMDRVGPY
PVLALSYLGAALVVGATGYLMGDVYTLAATVFLVGFGVAGAQNGLNLVSATIYPTAARVTGVSWAMASGRFGSIVGSMLG
AWMIAAAGSPETFFIWLALPVLVGSAAIFLLYRLSLSRRASAHDPALPN

Sequences:

>Translated_449_residues
MSQNTARISEIIDNAKVSPYQILILSLCFLIVLLDGFDTAIIGYIAPALREEWDLLPAQLSPAFGAGLFGLLLGSLIFGP
VADAIGRKRVLLVSILIFGGGTLASAYTHSIESLTLLRLITGIGLGGAMPTCITLSSEYSPARRRMIMVTLSWSGFTAGL
ALGGLLAGQIIPVFGWRGVLMLGGIAPLMLLPLLAWQMPESVRFMASSPKHAAGLRQVVERITGKSWAGVTILDDERPTQ
ARSPMTHLFTEGRAVRTLLLWAAFFCSLFVFYQLTSWLPSILKDAGYDIVHASRIGAMLPLGGTLGAILMALLMDRVGPY
PVLALSYLGAALVVGATGYLMGDVYTLAATVFLVGFGVAGAQNGLNLVSATIYPTAARVTGVSWAMASGRFGSIVGSMLG
AWMIAAAGSPETFFIWLALPVLVGSAAIFLLYRLSLSRRASAHDPALPN
>Mature_448_residues
SQNTARISEIIDNAKVSPYQILILSLCFLIVLLDGFDTAIIGYIAPALREEWDLLPAQLSPAFGAGLFGLLLGSLIFGPV
ADAIGRKRVLLVSILIFGGGTLASAYTHSIESLTLLRLITGIGLGGAMPTCITLSSEYSPARRRMIMVTLSWSGFTAGLA
LGGLLAGQIIPVFGWRGVLMLGGIAPLMLLPLLAWQMPESVRFMASSPKHAAGLRQVVERITGKSWAGVTILDDERPTQA
RSPMTHLFTEGRAVRTLLLWAAFFCSLFVFYQLTSWLPSILKDAGYDIVHASRIGAMLPLGGTLGAILMALLMDRVGPYP
VLALSYLGAALVVGATGYLMGDVYTLAATVFLVGFGVAGAQNGLNLVSATIYPTAARVTGVSWAMASGRFGSIVGSMLGA
WMIAAAGSPETFFIWLALPVLVGSAAIFLLYRLSLSRRASAHDPALPN

Specific function: Could Be A Transporter For 3-Phenylpropionate (Hydrocinnamic Acid). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=430, Percent_Identity=23.4883720930233, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI24497476, Length=400, Percent_Identity=26.25, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI20070188, Length=400, Percent_Identity=26.25, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI213021148, Length=405, Percent_Identity=23.9506172839506, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI11415038, Length=362, Percent_Identity=27.0718232044199, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI8923870, Length=365, Percent_Identity=26.3013698630137, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI7662270, Length=332, Percent_Identity=23.4939759036145, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI4506999, Length=335, Percent_Identity=27.1641791044776, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI24497478, Length=188, Percent_Identity=31.3829787234043, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI24497480, Length=186, Percent_Identity=31.1827956989247, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI166064021, Length=366, Percent_Identity=25.9562841530055, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI23510410, Length=315, Percent_Identity=27.3015873015873, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI262399383, Length=264, Percent_Identity=26.1363636363636, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI296080721, Length=420, Percent_Identity=24.7619047619048, Blast_Score=66, Evalue=6e-11,
Organism=Homo sapiens, GI296080719, Length=402, Percent_Identity=25.1243781094527, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI24497499, Length=402, Percent_Identity=25.1243781094527, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI87081723, Length=428, Percent_Identity=31.7757009345794, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI87082159, Length=406, Percent_Identity=29.3103448275862, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1786229, Length=375, Percent_Identity=25.6, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1788074, Length=370, Percent_Identity=25.4054054054054, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87082231, Length=198, Percent_Identity=28.2828282828283, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI87082404, Length=307, Percent_Identity=24.1042345276873, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789312, Length=419, Percent_Identity=26.491646778043, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1788068, Length=270, Percent_Identity=25.5555555555556, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI32564663, Length=459, Percent_Identity=25.2723311546841, Blast_Score=104, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24640198, Length=477, Percent_Identity=21.1740041928721, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640196, Length=477, Percent_Identity=21.1740041928721, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640200, Length=477, Percent_Identity=21.1740041928721, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19922874, Length=387, Percent_Identity=23.2558139534884, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47550; Mature: 47419

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNTARISEIIDNAKVSPYQILILSLCFLIVLLDGFDTAIIGYIAPALREEWDLLPAQL
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHC
SPAFGAGLFGLLLGSLIFGPVADAIGRKRVLLVSILIFGGGTLASAYTHSIESLTLLRLI
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
TGIGLGGAMPTCITLSSEYSPARRRMIMVTLSWSGFTAGLALGGLLAGQIIPVFGWRGVL
HHCCCCCCCCCEEEECCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHH
MLGGIAPLMLLPLLAWQMPESVRFMASSPKHAAGLRQVVERITGKSWAGVTILDDERPTQ
HHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCC
ARSPMTHLFTEGRAVRTLLLWAAFFCSLFVFYQLTSWLPSILKDAGYDIVHASRIGAMLP
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCC
LGGTLGAILMALLMDRVGPYPVLALSYLGAALVVGATGYLMGDVYTLAATVFLVGFGVAG
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AQNGLNLVSATIYPTAARVTGVSWAMASGRFGSIVGSMLGAWMIAAAGSPETFFIWLALP
CCCCCHHHEEECCCCHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
VLVGSAAIFLLYRLSLSRRASAHDPALPN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SQNTARISEIIDNAKVSPYQILILSLCFLIVLLDGFDTAIIGYIAPALREEWDLLPAQL
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHC
SPAFGAGLFGLLLGSLIFGPVADAIGRKRVLLVSILIFGGGTLASAYTHSIESLTLLRLI
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
TGIGLGGAMPTCITLSSEYSPARRRMIMVTLSWSGFTAGLALGGLLAGQIIPVFGWRGVL
HHCCCCCCCCCEEEECCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHH
MLGGIAPLMLLPLLAWQMPESVRFMASSPKHAAGLRQVVERITGKSWAGVTILDDERPTQ
HHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCC
ARSPMTHLFTEGRAVRTLLLWAAFFCSLFVFYQLTSWLPSILKDAGYDIVHASRIGAMLP
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCC
LGGTLGAILMALLMDRVGPYPVLALSYLGAALVVGATGYLMGDVYTLAATVFLVGFGVAG
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AQNGLNLVSATIYPTAARVTGVSWAMASGRFGSIVGSMLGAWMIAAAGSPETFFIWLALP
CCCCCHHHEEECCCCHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
VLVGSAAIFLLYRLSLSRRASAHDPALPN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8063101 [H]