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The map label for this gene is iciA [H]

Identifier: 157372159

GI number: 157372159

Start: 4348748

End: 4349641

Strand: Direct

Name: iciA [H]

Synonym: Spro_3925

Alternate gene names: 157372159

Gene position: 4348748-4349641 (Clockwise)

Preceding gene: 157372156

Following gene: 157372163

Centisome position: 79.81

GC content: 59.73

Gene sequence:

>894_bases
ATGAAACGCCCAGACTATCGAACGCTGCAAGCGCTGGACGCGGTGATCCGTGAGCGTGGCTTTGAGCGCGCCGCGCAAAA
ACTTTGCATCACACAATCGGCAGTATCCCAGCGCATCAAGCAGTTGGAAAATCTGTTTGGCCAACCGCTGTTGGTGCGTA
CCGTGCCGCCACGCCCGACCGAGCAGGGCCAAAAACTGCTGGCGCTGTTGCACCAGGTGGAATTACTGGAAGAAGAGTGG
CTGGGCAACGATACCGGCATTGATACGCCGCTGCTGCTGTCGCTGGCGGTTAACGCCGACAGCCTGGCGACCTGGCTGCT
ACCGGCATTGAAACCGGTGCTGACAGATTCCCCTATCCGCCTGAACCTGCAGGTAGAAGACGAAACCCGCACTCAGGAAC
GTCTGCGCCGGGGTGAAGTGGTTGGCGCTGTGAGTATCCAGCCACAGCCGCTGCCAAGCTGCCTGGTAGATCAACTGGGC
GCGCTGGATTATTTGTTTGTCGCCTCGAAGGGTTTTGCCGAGCGTTACTTCCCGAACGGGGTTACGCGCTCAGCGCTACT
CAAAGCGCCGGCGGTGGCGTTCGATCATCTCGACGACATGCACCAGGCTTTCCTGCAGCAGAACTTTGATCTGTCGCCGG
GGAGCGTGCCCTGCCATATAGTGAACTCGTCGGAAGCCTTCGTGCAGTTGGCACGCCAGGGCACCACCTGCTGTATGATC
CCGCATCTGCAGATTGAGAAAGAGTTGGCTTCGGGCGAACTGATCGATCTGACGCCGGGTCTGTTCCAGCGCCGCATGCT
GTTCTGGCACCGCTTCGCGCCGGAGAGCCGCATGATGCGTAAAGTGACCGATGCCCTGCTGGAGCACGGCCGCCAGGTTC
TGCGTCAGGATTAA

Upstream 100 bases:

>100_bases
AGGCGCGGTGCGGCAAGATGGCCCAGTGATTTTCCACCTGTAATACACCAGAGATGTGGCATAGTGCCTGTATCAATTTA
CTTTAAGGGATATCTTTCCG

Downstream 100 bases:

>100_bases
ATAGAAAAGGTTCGCCGCGGCGAACCTTTTGAGGTTACTGACAAAGTTGTTTTTAAGCCCGAGATACACGGGCCTAGCGC
ACCGAGGGGCACTCCGACGG

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLENLFGQPLLVRTVPPRPTEQGQKLLALLHQVELLEEEW
LGNDTGIDTPLLLSLAVNADSLATWLLPALKPVLTDSPIRLNLQVEDETRTQERLRRGEVVGAVSIQPQPLPSCLVDQLG
ALDYLFVASKGFAERYFPNGVTRSALLKAPAVAFDHLDDMHQAFLQQNFDLSPGSVPCHIVNSSEAFVQLARQGTTCCMI
PHLQIEKELASGELIDLTPGLFQRRMLFWHRFAPESRMMRKVTDALLEHGRQVLRQD

Sequences:

>Translated_297_residues
MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLENLFGQPLLVRTVPPRPTEQGQKLLALLHQVELLEEEW
LGNDTGIDTPLLLSLAVNADSLATWLLPALKPVLTDSPIRLNLQVEDETRTQERLRRGEVVGAVSIQPQPLPSCLVDQLG
ALDYLFVASKGFAERYFPNGVTRSALLKAPAVAFDHLDDMHQAFLQQNFDLSPGSVPCHIVNSSEAFVQLARQGTTCCMI
PHLQIEKELASGELIDLTPGLFQRRMLFWHRFAPESRMMRKVTDALLEHGRQVLRQD
>Mature_297_residues
MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLENLFGQPLLVRTVPPRPTEQGQKLLALLHQVELLEEEW
LGNDTGIDTPLLLSLAVNADSLATWLLPALKPVLTDSPIRLNLQVEDETRTQERLRRGEVVGAVSIQPQPLPSCLVDQLG
ALDYLFVASKGFAERYFPNGVTRSALLKAPAVAFDHLDDMHQAFLQQNFDLSPGSVPCHIVNSSEAFVQLARQGTTCCMI
PHLQIEKELASGELIDLTPGLFQRRMLFWHRFAPESRMMRKVTDALLEHGRQVLRQD

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=297, Percent_Identity=92.2558922558923, Blast_Score=558, Evalue=1e-160,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33431; Mature: 33431

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLENLFGQPLLVRTVPPRPT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
EQGQKLLALLHQVELLEEEWLGNDTGIDTPLLLSLAVNADSLATWLLPALKPVLTDSPIR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEE
LNLQVEDETRTQERLRRGEVVGAVSIQPQPLPSCLVDQLGALDYLFVASKGFAERYFPNG
EEEEECCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHCCHHHHHHHCCCHHHHHCCCC
VTRSALLKAPAVAFDHLDDMHQAFLQQNFDLSPGSVPCHIVNSSEAFVQLARQGTTCCMI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHCCCEEEEE
PHLQIEKELASGELIDLTPGLFQRRMLFWHRFAPESRMMRKVTDALLEHGRQVLRQD
CCHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLENLFGQPLLVRTVPPRPT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
EQGQKLLALLHQVELLEEEWLGNDTGIDTPLLLSLAVNADSLATWLLPALKPVLTDSPIR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEE
LNLQVEDETRTQERLRRGEVVGAVSIQPQPLPSCLVDQLGALDYLFVASKGFAERYFPNG
EEEEECCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHCCHHHHHHHCCCHHHHHCCCC
VTRSALLKAPAVAFDHLDDMHQAFLQQNFDLSPGSVPCHIVNSSEAFVQLARQGTTCCMI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHCCCEEEEE
PHLQIEKELASGELIDLTPGLFQRRMLFWHRFAPESRMMRKVTDALLEHGRQVLRQD
CCHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA