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The map label for this gene is ygfZ [C]

Identifier: 157372133

GI number: 157372133

Start: 4318967

End: 4319959

Strand: Direct

Name: ygfZ [C]

Synonym: Spro_3899

Alternate gene names: 157372133

Gene position: 4318967-4319959 (Clockwise)

Preceding gene: 157372127

Following gene: 157372135

Centisome position: 79.26

GC content: 59.11

Gene sequence:

>993_bases
ATGGCGTATAAAATTCCATTTCCACCCCGTCAGCCTTCCGCTTCCTCCCATCTGCCCCTGACCCTGATCTCACTGGAAGA
TTGGGCGCTGGTGACATTGAACGGGCCGGACCGGGTGAAATACCTTCAGGGCCAGGTAACCGCCGATATCGAAGCGCTGC
CGGCCGATAGCCACGTGTTGTGCGGCCACTGTGATGCCAAAGGCAAAATGTGGAGCAACCTGCGTTTATTCCATCGCGGT
GAAGGCTTTGCCTATCTGGAACGCCGCAGCGTGCTGGATAGCCAACTGGCTGAAATCAAAAAGTACGCGGTATTTTCCAA
ACTCACCATTGCCGCCGACAGCGAAGCAGTGTTGCTGGGCGTAGCCGGTTTTCAGGCACGCGCGGCGCTGGCGGGCGTTT
TCAACAGCCTGCCGGATGCCGAACATCAGGTCGTGCAGGACGGTGAAACTACCCTGTTGCACTTTTCACTCCCGGCAGAA
CGTTTTCTGTTGGTCACCACCGCCGCCGTTGCCGAACAGTTGGTCGACAAACTGCACGAGCAGGCCGAATTGAACGACAG
CCAGCAGTGGCTGACGCTGGATATCGAAGCCGGCTACCCGGTGATTGACGCCGCCAACAGCGGGCAGTTTATCCCACAGG
CCACCAACCTGCAGGCGCTGGATGGCATCAGCTTCAGCAAGGGCTGCTACACCGGTCAGGAAATGGTGGCGCGCGCCAAG
TTCCGCGGGGCCAACAAACGGGCATTGTACTGGCTGGAAGGCAAGGCTGGGCGAGTGCCGCAGGCCGCTGAAGACGTGGA
ATTGCAGTTGGGTGAAAACTGGCGCCGTACCGGTACGGTGCTGAGCGCTGCCAGGTTGGCCGACGGCACGCTGTGGGTAC
AAGTGGTGCTGAATAACGATCTGGACGCCGACAGCAAGCTGCGGGTTCGCGACGATGCCACCAGTCAACTGGCCATCAAG
CCACTGCCATACTCACTGGCGGAAGAGAAATAA

Upstream 100 bases:

>100_bases
TGCGAGGCAAGCGTGAATAACAGTTTGCAAAGGCCGCCACCTCTTTTACCATGGGGCCAACACACCTTCAGGTAAGCTAA
ACGTTAAGCAGGATCTTACT

Downstream 100 bases:

>100_bases
AGAAGGGAGCAGTCAGAGCTTCCTGGTCTGTCTGCAATAGATTTCGCACTGCAGCCAACAACGCTGCAGTGCGAAAGATA
TCAGCGGGTATCAGACGTAC

Product: putative global regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MAYKIPFPPRQPSASSHLPLTLISLEDWALVTLNGPDRVKYLQGQVTADIEALPADSHVLCGHCDAKGKMWSNLRLFHRG
EGFAYLERRSVLDSQLAEIKKYAVFSKLTIAADSEAVLLGVAGFQARAALAGVFNSLPDAEHQVVQDGETTLLHFSLPAE
RFLLVTTAAVAEQLVDKLHEQAELNDSQQWLTLDIEAGYPVIDAANSGQFIPQATNLQALDGISFSKGCYTGQEMVARAK
FRGANKRALYWLEGKAGRVPQAAEDVELQLGENWRRTGTVLSAARLADGTLWVQVVLNNDLDADSKLRVRDDATSQLAIK
PLPYSLAEEK

Sequences:

>Translated_330_residues
MAYKIPFPPRQPSASSHLPLTLISLEDWALVTLNGPDRVKYLQGQVTADIEALPADSHVLCGHCDAKGKMWSNLRLFHRG
EGFAYLERRSVLDSQLAEIKKYAVFSKLTIAADSEAVLLGVAGFQARAALAGVFNSLPDAEHQVVQDGETTLLHFSLPAE
RFLLVTTAAVAEQLVDKLHEQAELNDSQQWLTLDIEAGYPVIDAANSGQFIPQATNLQALDGISFSKGCYTGQEMVARAK
FRGANKRALYWLEGKAGRVPQAAEDVELQLGENWRRTGTVLSAARLADGTLWVQVVLNNDLDADSKLRVRDDATSQLAIK
PLPYSLAEEK
>Mature_329_residues
AYKIPFPPRQPSASSHLPLTLISLEDWALVTLNGPDRVKYLQGQVTADIEALPADSHVLCGHCDAKGKMWSNLRLFHRGE
GFAYLERRSVLDSQLAEIKKYAVFSKLTIAADSEAVLLGVAGFQARAALAGVFNSLPDAEHQVVQDGETTLLHFSLPAER
FLLVTTAAVAEQLVDKLHEQAELNDSQQWLTLDIEAGYPVIDAANSGQFIPQATNLQALDGISFSKGCYTGQEMVARAKF
RGANKRALYWLEGKAGRVPQAAEDVELQLGENWRRTGTVLSAARLADGTLWVQVVLNNDLDADSKLRVRDDATSQLAIKP
LPYSLAEEK

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family

Homologues:

Organism=Escherichia coli, GI1789265, Length=328, Percent_Identity=69.8170731707317, Blast_Score=463, Evalue=1e-132,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): YGFZ_SERP5 (A8GIQ4)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001480122.1
- ProteinModelPortal:   A8GIQ4
- SMR:   A8GIQ4
- STRING:   A8GIQ4
- GeneID:   5605065
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3899
- eggNOG:   COG0354
- HOGENOM:   HBG700628
- OMA:   GAHCDPK
- ProtClustDB:   PRK09559
- BioCyc:   SPRO399741:SPRO_3899-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01175
- InterPro:   IPR017703
- InterPro:   IPR006222
- TIGRFAMs:   TIGR03317

Pfam domain/function: PF01571 GCV_T

EC number: NA

Molecular weight: Translated: 36138; Mature: 36007

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: BINDING 28-28 BINDING 190-190

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYKIPFPPRQPSASSHLPLTLISLEDWALVTLNGPDRVKYLQGQVTADIEALPADSHVL
CCEECCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCCEEEECCCEEECEEECCCCCEEE
CGHCDAKGKMWSNLRLFHRGEGFAYLERRSVLDSQLAEIKKYAVFSKLTIAADSEAVLLG
EEECCCCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEE
VAGFQARAALAGVFNSLPDAEHQVVQDGETTLLHFSLPAERFLLVTTAAVAEQLVDKLHE
ECCHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHH
QAELNDSQQWLTLDIEAGYPVIDAANSGQFIPQATNLQALDGISFSKGCYTGQEMVARAK
HHCCCCCCEEEEEEEECCCCEEECCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHH
FRGANKRALYWLEGKAGRVPQAAEDVELQLGENWRRTGTVLSAARLADGTLWVQVVLNND
HCCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEECCC
LDADSKLRVRDDATSQLAIKPLPYSLAEEK
CCCCCEEEEECCCCCEEEEECCCCHHCCCC
>Mature Secondary Structure 
AYKIPFPPRQPSASSHLPLTLISLEDWALVTLNGPDRVKYLQGQVTADIEALPADSHVL
CEECCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCCEEEECCCEEECEEECCCCCEEE
CGHCDAKGKMWSNLRLFHRGEGFAYLERRSVLDSQLAEIKKYAVFSKLTIAADSEAVLLG
EEECCCCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEE
VAGFQARAALAGVFNSLPDAEHQVVQDGETTLLHFSLPAERFLLVTTAAVAEQLVDKLHE
ECCHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHH
QAELNDSQQWLTLDIEAGYPVIDAANSGQFIPQATNLQALDGISFSKGCYTGQEMVARAK
HHCCCCCCEEEEEEEECCCCEEECCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHH
FRGANKRALYWLEGKAGRVPQAAEDVELQLGENWRRTGTVLSAARLADGTLWVQVVLNND
HCCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEECCC
LDADSKLRVRDDATSQLAIKPLPYSLAEEK
CCCCCEEEEECCCCCEEEEECCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA