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The map label for this gene is proS

Identifier: 157371993

GI number: 157371993

Start: 4154267

End: 4155985

Strand: Direct

Name: proS

Synonym: Spro_3758

Alternate gene names: 157371993

Gene position: 4154267-4155985 (Clockwise)

Preceding gene: 157371992

Following gene: 157371997

Centisome position: 76.24

GC content: 56.2

Gene sequence:

>1719_bases
ATGCGTACTAGCCAATATCTGCTCTCCACTCTGAAGGAGACACCTGCCGATGCCGAAGTGATCAGCCACCAGCTGATGCT
GCGCGCCGGGATGATTCGCAAGCTGGCCTCCGGTCTTTATACCTGGTTGCCAACCGGCCTGCGCGTTCTGAAAAAGGTTG
AAAACATCGTTCGCGAAGAAATGAACAATGCTAACGCGATCGAAGTTTGCATGCCGGTGGTACAGCCTGCCGATCTGTGG
CAGGAAAGCGGCCGTTGGGAACAGTACGGCCCTGAGCTGCTGCGCTTTGTCGATCGCGGCGATCGTCCATTCGTGCTGGG
TCCAACCCACGAAGAAGTGATCACCGACCTGATCCGTAACGAGATCAGCTCTTACAAGCAGCTGCCGCTGAACTTCTTCC
AGATTCAGACCAAGTTCCGTGATGAAGTCCGCCCACGTTTCGGCGTCATGCGCTCGCGTGAATTCCTGATGAAGGATGCT
TACTCGTTCCATACTTCGCAGGAATCGCTGCAGGCAACCTACGAAGCGATGTACGAAGCCTACAGCAAAATCTTCAGCCG
TATGGGCCTGGACTTCCGTCCGGTACATGCCGATACCGGCTCGATCGGCGGTAGTGCATCACACGAGTTCCAGGTACTGG
CCGACAGCGGTGAAGACGATATCGTGTTTTCCACCGGCTCCGACTTTGCCGCCAATATCGAACTGGCAGAAGCGGTTGCA
CCGGCTGAGCCGCGTGCGGCAGCCAGTGAAGAACTGCGCATTGTGGATACCCCGAACGCCAAGACCATTGCTGAACTGGT
AGAACAATTCCAGCTGCCGGTGACGAAAACCGTCAAGACGCTGCTGGTACGTGCTAAAGAAGAGAGCGGCCACAAGCTGG
TTGCCCTGTTGGTGCGTGGCGATCACGAACTAAACGAAATCAAAGCCGAGAAGCTGCCGCAGGTTGCTGCTCCGTTGGTC
TTTGCTACCGAAGAAGAGATCCGCGCTATCGTGGGTGCAGGCCCTGGTTCACTCGGCCCGGTCAATTTGCAGGTTCCCGT
GGTTGCCGATCGCAGCGTAGCGGCGATGAGTGATTTTGGCGCCGGTGCCAACATCGACGGCAAACACTACTTTGGCATCA
ACTGGGAGCGCGATCTGCCGCTGCCACAGGTTGCCGATATCCGTAACGTCGTTGAAGGCGATGCCAGCCCGGACGGTAAA
GGCACACTGCTGATCAAACGCGGTATCGAAGTGGGCCATATCTTCCAGCTCGGCACCAAATACTCGGAAGCGATGAAAGC
CACCGTTCAGGGTGAAGATGGCCGTAATCAGGTGCTGACCATGGGTTGTTACGGTATCGGGGTCACCCGCGTGGTCGCTG
CCGCTATCGAGCAGAACCATGACGAACGCGGTATCATTTGGCCTGACGCTATCGCGCCATTCCAGGTGGCTATTCTGCCA
ATGAACATGCACAAATCCTTCCGCGTGCAGGCGCTGGCGGAGGAGTTGTACAACACTCTGCGTTCCCACGGTATCGACGT
GATCCTCGACGACCGTAAAGAACGTCCGGGCGTGATGTTCGCGGATATGGAACTGATTGGCGTACCGCACTCTATCGTGA
TCGGCGACCGTAACCTCGACAGCGAAGAGATTGAGTACAAAAACCGCCGTGTCGGCGAGAAGCAAATGATTAAAACCGGC
GAAGTCATTGATTTCTTGCTGGGGCAGATCAAGCGCTAA

Upstream 100 bases:

>100_bases
AACCCGCTCGCACTGGTAAACTAAATCACTTTCGAAAATTTTGGCTGCCCTACTACTGGCAGCCGGTTGCAATTTGCAGT
TCTAACGGAACCAAAACACC

Downstream 100 bases:

>100_bases
TCCACTATCGCAGAAACTAAAAACCCGCCTAGGCGGGTTTTTTTGTAGCGGTTATTTGCTGTCGCAATTTTTACTGCGGT
CGAAGGCTACTTTACCGTCC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGLRVLKKVENIVREEMNNANAIEVCMPVVQPADLW
QESGRWEQYGPELLRFVDRGDRPFVLGPTHEEVITDLIRNEISSYKQLPLNFFQIQTKFRDEVRPRFGVMRSREFLMKDA
YSFHTSQESLQATYEAMYEAYSKIFSRMGLDFRPVHADTGSIGGSASHEFQVLADSGEDDIVFSTGSDFAANIELAEAVA
PAEPRAAASEELRIVDTPNAKTIAELVEQFQLPVTKTVKTLLVRAKEESGHKLVALLVRGDHELNEIKAEKLPQVAAPLV
FATEEEIRAIVGAGPGSLGPVNLQVPVVADRSVAAMSDFGAGANIDGKHYFGINWERDLPLPQVADIRNVVEGDASPDGK
GTLLIKRGIEVGHIFQLGTKYSEAMKATVQGEDGRNQVLTMGCYGIGVTRVVAAAIEQNHDERGIIWPDAIAPFQVAILP
MNMHKSFRVQALAEELYNTLRSHGIDVILDDRKERPGVMFADMELIGVPHSIVIGDRNLDSEEIEYKNRRVGEKQMIKTG
EVIDFLLGQIKR

Sequences:

>Translated_572_residues
MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGLRVLKKVENIVREEMNNANAIEVCMPVVQPADLW
QESGRWEQYGPELLRFVDRGDRPFVLGPTHEEVITDLIRNEISSYKQLPLNFFQIQTKFRDEVRPRFGVMRSREFLMKDA
YSFHTSQESLQATYEAMYEAYSKIFSRMGLDFRPVHADTGSIGGSASHEFQVLADSGEDDIVFSTGSDFAANIELAEAVA
PAEPRAAASEELRIVDTPNAKTIAELVEQFQLPVTKTVKTLLVRAKEESGHKLVALLVRGDHELNEIKAEKLPQVAAPLV
FATEEEIRAIVGAGPGSLGPVNLQVPVVADRSVAAMSDFGAGANIDGKHYFGINWERDLPLPQVADIRNVVEGDASPDGK
GTLLIKRGIEVGHIFQLGTKYSEAMKATVQGEDGRNQVLTMGCYGIGVTRVVAAAIEQNHDERGIIWPDAIAPFQVAILP
MNMHKSFRVQALAEELYNTLRSHGIDVILDDRKERPGVMFADMELIGVPHSIVIGDRNLDSEEIEYKNRRVGEKQMIKTG
EVIDFLLGQIKR
>Mature_572_residues
MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGLRVLKKVENIVREEMNNANAIEVCMPVVQPADLW
QESGRWEQYGPELLRFVDRGDRPFVLGPTHEEVITDLIRNEISSYKQLPLNFFQIQTKFRDEVRPRFGVMRSREFLMKDA
YSFHTSQESLQATYEAMYEAYSKIFSRMGLDFRPVHADTGSIGGSASHEFQVLADSGEDDIVFSTGSDFAANIELAEAVA
PAEPRAAASEELRIVDTPNAKTIAELVEQFQLPVTKTVKTLLVRAKEESGHKLVALLVRGDHELNEIKAEKLPQVAAPLV
FATEEEIRAIVGAGPGSLGPVNLQVPVVADRSVAAMSDFGAGANIDGKHYFGINWERDLPLPQVADIRNVVEGDASPDGK
GTLLIKRGIEVGHIFQLGTKYSEAMKATVQGEDGRNQVLTMGCYGIGVTRVVAAAIEQNHDERGIIWPDAIAPFQVAILP
MNMHKSFRVQALAEELYNTLRSHGIDVILDDRKERPGVMFADMELIGVPHSIVIGDRNLDSEEIEYKNRRVGEKQMIKTG
EVIDFLLGQIKR

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=237, Percent_Identity=43.8818565400844, Blast_Score=213, Evalue=3e-55,
Organism=Escherichia coli, GI1786392, Length=571, Percent_Identity=85.6392294220665, Blast_Score=1032, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=243, Percent_Identity=38.6831275720165, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI193203271, Length=115, Percent_Identity=35.6521739130435, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=549, Percent_Identity=30.6010928961749, Blast_Score=252, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24656200, Length=235, Percent_Identity=36.5957446808511, Blast_Score=177, Evalue=2e-44,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_SERP5 (A8GIB4)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479982.1
- STRING:   A8GIB4
- GeneID:   5603204
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3758
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   SPRO399741:SPRO_3758-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63578; Mature: 63578

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGLRVLKKVENIVREE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNNANAIEVCMPVVQPADLWQESGRWEQYGPELLRFVDRGDRPFVLGPTHEEVITDLIRN
HCCCCHHHHHHHHCCCHHHHHHCCCCHHHCHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
EISSYKQLPLNFFQIQTKFRDEVRPRFGVMRSREFLMKDAYSFHTSQESLQATYEAMYEA
HHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YSKIFSRMGLDFRPVHADTGSIGGSASHEFQVLADSGEDDIVFSTGSDFAANIELAEAVA
HHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCEECCCHHHHCC
PAEPRAAASEELRIVDTPNAKTIAELVEQFQLPVTKTVKTLLVRAKEESGHKLVALLVRG
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEEEEC
DHELNEIKAEKLPQVAAPLVFATEEEIRAIVGAGPGSLGPVNLQVPVVADRSVAAMSDFG
CCHHHHHHHHHCCHHHCCEEEECHHHHHHHHCCCCCCCCCEEEEEEEEECCCHHHHHHCC
AGANIDGKHYFGINWERDLPLPQVADIRNVVEGDASPDGKGTLLIKRGIEVGHIFQLGTK
CCCCCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCEEHHHHHHHH
YSEAMKATVQGEDGRNQVLTMGCYGIGVTRVVAAAIEQNHDERGIIWPDAIAPFQVAILP
HHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEE
MNMHKSFRVQALAEELYNTLRSHGIDVILDDRKERPGVMFADMELIGVPHSIVIGDRNLD
ECCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECEEEECCCCEEEEECCCCC
SEEIEYKNRRVGEKQMIKTGEVIDFLLGQIKR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGLRVLKKVENIVREE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNNANAIEVCMPVVQPADLWQESGRWEQYGPELLRFVDRGDRPFVLGPTHEEVITDLIRN
HCCCCHHHHHHHHCCCHHHHHHCCCCHHHCHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
EISSYKQLPLNFFQIQTKFRDEVRPRFGVMRSREFLMKDAYSFHTSQESLQATYEAMYEA
HHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YSKIFSRMGLDFRPVHADTGSIGGSASHEFQVLADSGEDDIVFSTGSDFAANIELAEAVA
HHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCEECCCHHHHCC
PAEPRAAASEELRIVDTPNAKTIAELVEQFQLPVTKTVKTLLVRAKEESGHKLVALLVRG
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEEEEC
DHELNEIKAEKLPQVAAPLVFATEEEIRAIVGAGPGSLGPVNLQVPVVADRSVAAMSDFG
CCHHHHHHHHHCCHHHCCEEEECHHHHHHHHCCCCCCCCCEEEEEEEEECCCHHHHHHCC
AGANIDGKHYFGINWERDLPLPQVADIRNVVEGDASPDGKGTLLIKRGIEVGHIFQLGTK
CCCCCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCEEHHHHHHHH
YSEAMKATVQGEDGRNQVLTMGCYGIGVTRVVAAAIEQNHDERGIIWPDAIAPFQVAILP
HHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEE
MNMHKSFRVQALAEELYNTLRSHGIDVILDDRKERPGVMFADMELIGVPHSIVIGDRNLD
ECCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECEEEECCCCEEEEECCCCC
SEEIEYKNRRVGEKQMIKTGEVIDFLLGQIKR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA