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The map label for this gene is yhcA [H]

Identifier: 157371950

GI number: 157371950

Start: 4104909

End: 4106288

Strand: Direct

Name: yhcA [H]

Synonym: Spro_3715

Alternate gene names: 157371950

Gene position: 4104909-4106288 (Clockwise)

Preceding gene: 157371948

Following gene: 157371951

Centisome position: 75.34

GC content: 59.93

Gene sequence:

>1380_bases
ATGCCCTATCGCATCAAAGTCGCAATCGTCTATCTGCTCGGATTTTTTGTCGATCTGATCAACATGTTTATCGCCAACGT
CGCTTATCCGTCGATCGGCCATGCGCTGCAGGCTTCGGTCGGTGAACTGGCCTGGGTCAGTAACGGTTATATTCTCGGCC
TGACGCTGGTGATCCCGCTCAGCGCCTGGCTGGCACAGCGCATCGGCGGACGACGGGTATTTTTGTTGTCGCTGACGCTG
TTTATGCTGGCGACAACCGCGGCGGGCTACGCCAGCTCAATTGGCGAGTTGATTGGTTGGCGGGTACTGCAAGGTATGGG
CGGCGGTTTGCTGATCCCTATCGGCCAAACGCTGACCTATCAACTGTATCGCAGCCACGAACGCGCCGGGCTTTCCGCCG
CCATTATGCTGGTCGGCCTGCTGGCACCGGCACTGTCACCGGCGCTGGGCGGGTTGATTGTCGACAGTCTTGACTGGCGT
TGGGTGTTCTTCGCCAACCTACCGCTGGCGGCGCTGGCGTTGCTGCTGGCCGCGCTATGGTTACGCCCGCAGGCGGCCGA
TCCGCAGCGCAGGCCGCTCGATCTCAATGGGCTGCTCAGCGCCTGCGCTGCGCTGACACTAATCCTGCTGGGTCTGACCC
GGCTGGGTGAAGCCGATGGCCTGACGCAGGGCATGGTGTTATTGGCTGCCGGGGCACTGATGCTGGCCTATTACCTGCGA
CGCAGTCTGCGCATTGCCCAACCGCTGCTGAATTTGCGGCTGATGCAGGATCCGTTGCTGCGCACTTCAATGATGATTTA
TCAATGCATTCCCGGCCTGTTTATCGGCGTCAGCCTGGTCGCCATGCTGTATCTGCAAAATCAATTGGGTATGCACGCCG
CACAGGTCGGTAGCCTGATGCTGCCCTGGTCGCTGGCCTCGTTTTTGGCCATCACGCTGACCGGCAAGAAGTTTAACCGC
CTTGGCCCGCGACCGCTGTTTATCGCCGGTTGCCTGCTGCAGGGGTTGGGCATTCTGGCGCTGTCGCAAATTACCCATGC
CGGGGATCATGGGTTGCAGCTGGCGGCCTTCGCCCTGATGGGCTTTGGCGGCAGCCTGTGCAGCAGCACCGCGCAAAGCA
GCGCTTTCCTGCAGATTGCCGATGAGCAACTGGCGGATGCCAGCGCACTGTGGAATATCAATCGACAGCTCAGTTTTTGC
CTGGGGGTCGCCCTGCTCAGCCTGCTGCTGAATCTGCTGCTTGAACATTTTTCCCCCGCCGTCGCTTACCAAAGCTGTTT
TTACCTGGCCGCCGCCAGCACGCTGATCCCACTGCTGCTGTGTCTGCGCATCGCTAACCGCGCCATCGTGCGCCATCTCA
ATACTCAACAGGAAAACTGA

Upstream 100 bases:

>100_bases
GCCCGTTATTGTCAAAATTATTTTGACAGTGTAATCGTTCGCAGCCGATTTATCCGCAGTTTCCCACCCGCTACACTGCG
GGCCTTCAACCGGAGGCCTC

Downstream 100 bases:

>100_bases
TGAACCGCTATTTTACCGAAGTGATCGACGCTCATGTCGCCATTGAAAACTGGCTGGAGAAAGGTGCAGGGGAAGAACAG
GCCTTATTGGCACGTTTTGA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MPYRIKVAIVYLLGFFVDLINMFIANVAYPSIGHALQASVGELAWVSNGYILGLTLVIPLSAWLAQRIGGRRVFLLSLTL
FMLATTAAGYASSIGELIGWRVLQGMGGGLLIPIGQTLTYQLYRSHERAGLSAAIMLVGLLAPALSPALGGLIVDSLDWR
WVFFANLPLAALALLLAALWLRPQAADPQRRPLDLNGLLSACAALTLILLGLTRLGEADGLTQGMVLLAAGALMLAYYLR
RSLRIAQPLLNLRLMQDPLLRTSMMIYQCIPGLFIGVSLVAMLYLQNQLGMHAAQVGSLMLPWSLASFLAITLTGKKFNR
LGPRPLFIAGCLLQGLGILALSQITHAGDHGLQLAAFALMGFGGSLCSSTAQSSAFLQIADEQLADASALWNINRQLSFC
LGVALLSLLLNLLLEHFSPAVAYQSCFYLAAASTLIPLLLCLRIANRAIVRHLNTQQEN

Sequences:

>Translated_459_residues
MPYRIKVAIVYLLGFFVDLINMFIANVAYPSIGHALQASVGELAWVSNGYILGLTLVIPLSAWLAQRIGGRRVFLLSLTL
FMLATTAAGYASSIGELIGWRVLQGMGGGLLIPIGQTLTYQLYRSHERAGLSAAIMLVGLLAPALSPALGGLIVDSLDWR
WVFFANLPLAALALLLAALWLRPQAADPQRRPLDLNGLLSACAALTLILLGLTRLGEADGLTQGMVLLAAGALMLAYYLR
RSLRIAQPLLNLRLMQDPLLRTSMMIYQCIPGLFIGVSLVAMLYLQNQLGMHAAQVGSLMLPWSLASFLAITLTGKKFNR
LGPRPLFIAGCLLQGLGILALSQITHAGDHGLQLAAFALMGFGGSLCSSTAQSSAFLQIADEQLADASALWNINRQLSFC
LGVALLSLLLNLLLEHFSPAVAYQSCFYLAAASTLIPLLLCLRIANRAIVRHLNTQQEN
>Mature_458_residues
PYRIKVAIVYLLGFFVDLINMFIANVAYPSIGHALQASVGELAWVSNGYILGLTLVIPLSAWLAQRIGGRRVFLLSLTLF
MLATTAAGYASSIGELIGWRVLQGMGGGLLIPIGQTLTYQLYRSHERAGLSAAIMLVGLLAPALSPALGGLIVDSLDWRW
VFFANLPLAALALLLAALWLRPQAADPQRRPLDLNGLLSACAALTLILLGLTRLGEADGLTQGMVLLAAGALMLAYYLRR
SLRIAQPLLNLRLMQDPLLRTSMMIYQCIPGLFIGVSLVAMLYLQNQLGMHAAQVGSLMLPWSLASFLAITLTGKKFNRL
GPRPLFIAGCLLQGLGILALSQITHAGDHGLQLAAFALMGFGGSLCSSTAQSSAFLQIADEQLADASALWNINRQLSFCL
GVALLSLLLNLLLEHFSPAVAYQSCFYLAAASTLIPLLLCLRIANRAIVRHLNTQQEN

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=463, Percent_Identity=25.9179265658747, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1788710, Length=410, Percent_Identity=26.5853658536585, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI87081983, Length=418, Percent_Identity=25.5980861244019, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789042, Length=400, Percent_Identity=26.25, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1788392, Length=414, Percent_Identity=28.5024154589372, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6325455, Length=232, Percent_Identity=22.4137931034483, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=145, Percent_Identity=29.6551724137931, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=392, Percent_Identity=23.7244897959184, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49361; Mature: 49229

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYRIKVAIVYLLGFFVDLINMFIANVAYPSIGHALQASVGELAWVSNGYILGLTLVIPL
CCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEECCCCHHHHHHHHHH
SAWLAQRIGGRRVFLLSLTLFMLATTAAGYASSIGELIGWRVLQGMGGGLLIPIGQTLTY
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHH
QLYRSHERAGLSAAIMLVGLLAPALSPALGGLIVDSLDWRWVFFANLPLAALALLLAALW
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHH
LRPQAADPQRRPLDLNGLLSACAALTLILLGLTRLGEADGLTQGMVLLAAGALMLAYYLR
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
RSLRIAQPLLNLRLMQDPLLRTSMMIYQCIPGLFIGVSLVAMLYLQNQLGMHAAQVGSLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCH
LPWSLASFLAITLTGKKFNRLGPRPLFIAGCLLQGLGILALSQITHAGDHGLQLAAFALM
HHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
GFGGSLCSSTAQSSAFLQIADEQLADASALWNINRQLSFCLGVALLSLLLNLLLEHFSPA
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
VAYQSCFYLAAASTLIPLLLCLRIANRAIVRHLNTQQEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PYRIKVAIVYLLGFFVDLINMFIANVAYPSIGHALQASVGELAWVSNGYILGLTLVIPL
CEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEECCCCHHHHHHHHHH
SAWLAQRIGGRRVFLLSLTLFMLATTAAGYASSIGELIGWRVLQGMGGGLLIPIGQTLTY
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHH
QLYRSHERAGLSAAIMLVGLLAPALSPALGGLIVDSLDWRWVFFANLPLAALALLLAALW
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHH
LRPQAADPQRRPLDLNGLLSACAALTLILLGLTRLGEADGLTQGMVLLAAGALMLAYYLR
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
RSLRIAQPLLNLRLMQDPLLRTSMMIYQCIPGLFIGVSLVAMLYLQNQLGMHAAQVGSLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCH
LPWSLASFLAITLTGKKFNRLGPRPLFIAGCLLQGLGILALSQITHAGDHGLQLAAFALM
HHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
GFGGSLCSSTAQSSAFLQIADEQLADASALWNINRQLSFCLGVALLSLLLNLLLEHFSPA
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
VAYQSCFYLAAASTLIPLLLCLRIANRAIVRHLNTQQEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]