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The map label for this gene is nupC [H]

Identifier: 157371872

GI number: 157371872

Start: 4021081

End: 4022265

Strand: Reverse

Name: nupC [H]

Synonym: Spro_3637

Alternate gene names: 157371872

Gene position: 4022265-4021081 (Counterclockwise)

Preceding gene: 157371873

Following gene: 157371871

Centisome position: 73.82

GC content: 47.76

Gene sequence:

>1185_bases
ATGACGCAGGTTATTCACTTTATCCTCTCGTGTCTGTTAGTGACGATAATGGCATTGATGGTCAGCCATGACCGAAAGTT
AATTAAGATCCGCTATATTATTCAGCTGCTGGTGATTGAAGTATTACTTGCCTGGCTGTTCCTTCGTTCTGATATGGGTA
CCGGATTTACGGCACAGGTTTCTACGGTGTTTAACGGCTTGATGCTGTTTGCCGGTGAAGGCACTGACTTTTTATTCGGC
GGAATGCGGGCGCAAGGACTGGCATTCTTCCTTCTCAATGTATTGTGTCCTATTATTTTTATTTCTGCATTGATCGGTAT
TATTCAGCACATCAATCTGTTGCCGATTATTATCCGGGTAGTGGGGACGGTGTTGTCCAAAGTCAATGGCATGGGCAAGT
TGGAGTCTTTTAATGCTGTCAGCGCATTATTGCTGGGGCAGTCAGAGAACTTTATCGTTTATAAAGACGTGCTGGGCAAG
ATGTCCGAAAATCGCATTTACACTATGGCAGCCACCGCGATGTCAACCGTCTCGGTTTTTATGGTCGGTGCCTATATCAA
TATGGTTGATGCCCGCTATGTCGTAGTGTCATTGATTCTGAATATGTTTAGCACATTTATCGTGCTGTCACTGATTAATC
CGTATAGTGTCGATTTGGAGGAAGAGGTTGAGATGCAGGATCTGCATCGTGGACAAAGTTTATTTGAAATGTTGGGCGAA
TATATTCTTGCCGGCTTTAAGGTCGCAGTAATCATTGCCGCCATGCTGCTGGGCTTTATTGCCCTGATCGCCGCCATCAA
CGCGTTGTTTAGCAGCCTGTTTGGCATCAGTTTTCAAGGCTTGCTGGGCTATCTGCTTTATCCATTTGCCTGGGTGCTTG
GCGTGCCGGCTCATGAGGCGCAGCAGGTTGGCAGCATCATGGCGACCAAGATTGTCGCCAACGAGTTTGTCGCCATGACT
GAGTTGCAACAGATTAAAGCCGTGCTGTCGCCCCGCGCCGAAGGGATCTTGTCGGTATTCCTGATTTCCTTTGCCAACTT
CACCTCGGTGGGCATTGTGGCGGGTGCGATTAAAGCATTGAACGAGGAGCAGGGGAACGTGGTTTCCCGTTTCAGTATGA
AACTGCTGTATGGCTCAACGCTGGTGAGTTTCTTATCGGCAGCGGTAGCGGGCTTGGTCATTTAA

Upstream 100 bases:

>100_bases
TTGTTTACAGACGGTGTTATGTGATGTGAGTCTATATGATTGTTATTTGCTCTCATGGTATACCTTGAAAAAGGACGGTT
ATTATACTATGGAGTGTCGT

Downstream 100 bases:

>100_bases
ACCCTCTGGCAGGCTAGATTAAGGAATATTTATCCCGGCGGTATTTAAAATCAACCGACATAAGGAGGTGTTGCAATGAA
GATTTTATTTATTGGTGAGT

Product: Na+ dependent nucleoside transporter

Products: uridine [Cytoplasm]; thymidine [Cytoplasm]; inosine [Cytoplasm]; Deoxyuridine [Cytoplasm]; deoxyinosine [Cytoplasm]; deoxycytidine [Cytoplasm]; deoxyadenosine [Cytoplasm]; cytidine [Cytoplasm]; Proton [Cytoplasm]; adenosine [Cytoplasm] [C]

Alternate protein names: Nucleoside-transport system protein nupC [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MTQVIHFILSCLLVTIMALMVSHDRKLIKIRYIIQLLVIEVLLAWLFLRSDMGTGFTAQVSTVFNGLMLFAGEGTDFLFG
GMRAQGLAFFLLNVLCPIIFISALIGIIQHINLLPIIIRVVGTVLSKVNGMGKLESFNAVSALLLGQSENFIVYKDVLGK
MSENRIYTMAATAMSTVSVFMVGAYINMVDARYVVVSLILNMFSTFIVLSLINPYSVDLEEEVEMQDLHRGQSLFEMLGE
YILAGFKVAVIIAAMLLGFIALIAAINALFSSLFGISFQGLLGYLLYPFAWVLGVPAHEAQQVGSIMATKIVANEFVAMT
ELQQIKAVLSPRAEGILSVFLISFANFTSVGIVAGAIKALNEEQGNVVSRFSMKLLYGSTLVSFLSAAVAGLVI

Sequences:

>Translated_394_residues
MTQVIHFILSCLLVTIMALMVSHDRKLIKIRYIIQLLVIEVLLAWLFLRSDMGTGFTAQVSTVFNGLMLFAGEGTDFLFG
GMRAQGLAFFLLNVLCPIIFISALIGIIQHINLLPIIIRVVGTVLSKVNGMGKLESFNAVSALLLGQSENFIVYKDVLGK
MSENRIYTMAATAMSTVSVFMVGAYINMVDARYVVVSLILNMFSTFIVLSLINPYSVDLEEEVEMQDLHRGQSLFEMLGE
YILAGFKVAVIIAAMLLGFIALIAAINALFSSLFGISFQGLLGYLLYPFAWVLGVPAHEAQQVGSIMATKIVANEFVAMT
ELQQIKAVLSPRAEGILSVFLISFANFTSVGIVAGAIKALNEEQGNVVSRFSMKLLYGSTLVSFLSAAVAGLVI
>Mature_393_residues
TQVIHFILSCLLVTIMALMVSHDRKLIKIRYIIQLLVIEVLLAWLFLRSDMGTGFTAQVSTVFNGLMLFAGEGTDFLFGG
MRAQGLAFFLLNVLCPIIFISALIGIIQHINLLPIIIRVVGTVLSKVNGMGKLESFNAVSALLLGQSENFIVYKDVLGKM
SENRIYTMAATAMSTVSVFMVGAYINMVDARYVVVSLILNMFSTFIVLSLINPYSVDLEEEVEMQDLHRGQSLFEMLGEY
ILAGFKVAVIIAAMLLGFIALIAAINALFSSLFGISFQGLLGYLLYPFAWVLGVPAHEAQQVGSIMATKIVANEFVAMTE
LQQIKAVLSPRAEGILSVFLISFANFTSVGIVAGAIKALNEEQGNVVSRFSMKLLYGSTLVSFLSAAVAGLVI

Specific function: Transports nucleosides with a high affinity except guanosine and deoxyguanosine. Driven by a proton motive force [H]

COG id: COG1972

COG function: function code F; Nucleoside permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family [H]

Homologues:

Organism=Homo sapiens, GI42542381, Length=423, Percent_Identity=23.4042553191489, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI227116277, Length=426, Percent_Identity=24.8826291079812, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI11545853, Length=350, Percent_Identity=23.1428571428571, Blast_Score=99, Evalue=5e-21,
Organism=Escherichia coli, GI1788737, Length=400, Percent_Identity=70.25, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI1788485, Length=414, Percent_Identity=27.536231884058, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1788488, Length=400, Percent_Identity=26.5, Blast_Score=142, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17560276, Length=392, Percent_Identity=24.234693877551, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71991794, Length=389, Percent_Identity=22.107969151671, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI45552519, Length=406, Percent_Identity=23.6453201970443, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI45552517, Length=406, Percent_Identity=23.6453201970443, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19921868, Length=406, Percent_Identity=23.6453201970443, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI281360430, Length=339, Percent_Identity=23.0088495575221, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008276
- InterPro:   IPR018270
- InterPro:   IPR011657
- InterPro:   IPR002668 [H]

Pfam domain/function: PF07662 Nucleos_tra2_C; PF01773 Nucleos_tra2_N [H]

EC number: NA

Molecular weight: Translated: 42874; Mature: 42743

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQVIHFILSCLLVTIMALMVSHDRKLIKIRYIIQLLVIEVLLAWLFLRSDMGTGFTAQV
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
STVFNGLMLFAGEGTDFLFGGMRAQGLAFFLLNVLCPIIFISALIGIIQHINLLPIIIRV
HHHHHHHHHEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGTVLSKVNGMGKLESFNAVSALLLGQSENFIVYKDVLGKMSENRIYTMAATAMSTVSVF
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEHHHHCCCCCCCEEHHHHHHHHHHHHH
MVGAYINMVDARYVVVSLILNMFSTFIVLSLINPYSVDLEEEVEMQDLHRGQSLFEMLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YILAGFKVAVIIAAMLLGFIALIAAINALFSSLFGISFQGLLGYLLYPFAWVLGVPAHEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHH
QQVGSIMATKIVANEFVAMTELQQIKAVLSPRAEGILSVFLISFANFTSVGIVAGAIKAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NEEQGNVVSRFSMKLLYGSTLVSFLSAAVAGLVI
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQVIHFILSCLLVTIMALMVSHDRKLIKIRYIIQLLVIEVLLAWLFLRSDMGTGFTAQV
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
STVFNGLMLFAGEGTDFLFGGMRAQGLAFFLLNVLCPIIFISALIGIIQHINLLPIIIRV
HHHHHHHHHEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGTVLSKVNGMGKLESFNAVSALLLGQSENFIVYKDVLGKMSENRIYTMAATAMSTVSVF
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEHHHHCCCCCCCEEHHHHHHHHHHHHH
MVGAYINMVDARYVVVSLILNMFSTFIVLSLINPYSVDLEEEVEMQDLHRGQSLFEMLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YILAGFKVAVIIAAMLLGFIALIAAINALFSSLFGISFQGLLGYLLYPFAWVLGVPAHEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHH
QQVGSIMATKIVANEFVAMTELQQIKAVLSPRAEGILSVFLISFANFTSVGIVAGAIKAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NEEQGNVVSRFSMKLLYGSTLVSFLSAAVAGLVI
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine [Periplasm]; thymidine [Periplasm]; inosine [Periplasm]; Deoxyuridine [Periplasm]; deoxyinosine [Periplasm]; deoxycytidine [Periplasm]; deoxyadenosine [Periplasm]; cytidine [Periplasm]; Proton [Periplasm]; adenosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uridine [Periplasm] = Proton [Cytoplasm] + uridine [Cytoplasm] Proton [Periplasm] + thymidine [Periplasm] = Proton [Cytoplasm] + thymidine [Cytoplasm] Proton [Periplasm] + inosine [Periplasm] = Proton [Cytoplasm] + inosine [Cytoplasm]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]