Click here to switch to the map view.

The map label for this gene is iscA

Identifier: 157371860

GI number: 157371860

Start: 4010968

End: 4011291

Strand: Reverse

Name: iscA

Synonym: Spro_3625

Alternate gene names: 157371860

Gene position: 4011291-4010968 (Counterclockwise)

Preceding gene: 157371861

Following gene: 157371859

Centisome position: 73.62

GC content: 51.23

Gene sequence:

>324_bases
ATGTCAATTACCATGAGCGACAGCGCTGCACAGCGCGTTCAGGCATTTATGAATAACCGTGGCAAAGGCCTTGGCCTGCG
TCTGGGGGTGAGAACCTCTGGCTGCTCCGGGATGGCGTATGTGCTGGAATTTGTTGACGATATGAACGATGACGACATCG
TTTTTGAAAACAAAGGCGTTAAGGTGATCATTGACGGCAAGAGCCTGGTCTACCTTGACGGCACCGAGCTTGATTTCGTT
AAGGAAGGCCTGAACGAAGGCTTCAAGTTCAACAACCCCAATGTCTCAAGCGAGTGTGGCTGCGGCGAAAGTTTCAACGT
CTGA

Upstream 100 bases:

>100_bases
AAAGCAGCGATTGCCGACTACAAGAGCAAACACAGCGCCAAGTAATTATTTCTGAGTCATTTAGCCCGGCCTTGGTTGGG
CTTTGAAGTGTGAGGTTGTG

Downstream 100 bases:

>100_bases
CAGTCCATCGTGCTCCCCCGCCAGGTGCGGGGGAACTTCATCCGATAACGCCCAGAGCACGCTATGGATTACTTTACTTT
ATTCGGGCTGCCAGTTCGCT

Product: iron-sulfur cluster assembly protein

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein

Number of amino acids: Translated: 107; Mature: 106

Protein sequence:

>107_residues
MSITMSDSAAQRVQAFMNNRGKGLGLRLGVRTSGCSGMAYVLEFVDDMNDDDIVFENKGVKVIIDGKSLVYLDGTELDFV
KEGLNEGFKFNNPNVSSECGCGESFNV

Sequences:

>Translated_107_residues
MSITMSDSAAQRVQAFMNNRGKGLGLRLGVRTSGCSGMAYVLEFVDDMNDDDIVFENKGVKVIIDGKSLVYLDGTELDFV
KEGLNEGFKFNNPNVSSECGCGESFNV
>Mature_106_residues
SITMSDSAAQRVQAFMNNRGKGLGLRLGVRTSGCSGMAYVLEFVDDMNDDDIVFENKGVKVIIDGKSLVYLDGTELDFVK
EGLNEGFKFNNPNVSSECGCGESFNV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family

Homologues:

Organism=Homo sapiens, GI52426768, Length=106, Percent_Identity=39.622641509434, Blast_Score=100, Evalue=2e-22,
Organism=Homo sapiens, GI160420328, Length=106, Percent_Identity=36.7924528301887, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788877, Length=107, Percent_Identity=79.4392523364486, Blast_Score=179, Evalue=5e-47,
Organism=Escherichia coli, GI1787974, Length=105, Percent_Identity=43.8095238095238, Blast_Score=110, Evalue=1e-26,
Organism=Escherichia coli, GI1786351, Length=110, Percent_Identity=38.1818181818182, Blast_Score=75, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI193209025, Length=106, Percent_Identity=36.7924528301887, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI25146411, Length=106, Percent_Identity=36.7924528301887, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71998452, Length=106, Percent_Identity=33.9622641509434, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323001, Length=105, Percent_Identity=39.0476190476191, Blast_Score=86, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24642263, Length=106, Percent_Identity=39.622641509434, Blast_Score=95, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24649697, Length=108, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISCA_SERP5 (A8GHY1)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479849.1
- ProteinModelPortal:   A8GHY1
- SMR:   A8GHY1
- STRING:   A8GHY1
- GeneID:   5606010
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3625
- eggNOG:   COG0316
- HOGENOM:   HBG730174
- OMA:   ELAPEDQ
- ProtClustDB:   PRK09502
- BioCyc:   SPRO399741:SPRO_3625-MONOMER
- HAMAP:   MF_01429
- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302
- Gene3D:   G3DSA:2.60.300.12
- PANTHER:   PTHR10072
- TIGRFAMs:   TIGR00049
- TIGRFAMs:   TIGR02011

Pfam domain/function: PF01521 Fe-S_biosyn; SSF89360 HesB_yadR_yfhF-like

EC number: NA

Molecular weight: Translated: 11615; Mature: 11484

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITMSDSAAQRVQAFMNNRGKGLGLRLGVRTSGCSGMAYVLEFVDDMNDDDIVFENKGV
CEEECCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEECCCE
KVIIDGKSLVYLDGTELDFVKEGLNEGFKFNNPNVSSECGCGESFNV
EEEECCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SITMSDSAAQRVQAFMNNRGKGLGLRLGVRTSGCSGMAYVLEFVDDMNDDDIVFENKGV
EEECCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEECCCE
KVIIDGKSLVYLDGTELDFVKEGLNEGFKFNNPNVSSECGCGESFNV
EEEECCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA