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The map label for this gene is rlmN

Identifier: 157371847

GI number: 157371847

Start: 3991309

End: 3992505

Strand: Reverse

Name: rlmN

Synonym: Spro_3612

Alternate gene names: 157371847

Gene position: 3992505-3991309 (Counterclockwise)

Preceding gene: 157371848

Following gene: 157371846

Centisome position: 73.27

GC content: 54.72

Gene sequence:

>1197_bases
ATGTTAGAACCCATCACGTCCGAGCACATCGTGTCTGAAAATAATTCGCTGACTACTCAATCCGTTAACGCGGAACAACC
GGCTGTGGCCAAAATTAACCTGCTGGATTTGAACCGTCAGCAACTGCGTGAGTTTTTCGCCAAAATGGGCGAGAAACCCT
TCCGTGCCGATCAGGTCATGAAGTGGATGTACCACTATTGCTGCGATGACTTTGAGCAGATGACCGACATTAACAAGGTC
CTGCGCAATAAATTGCAGAGCGTCGCCGAGATCCGTGCGCCAGAAGTGGCGGAAGAACAACGCTCAGCCGATGGCACCAT
CAAATGGGCGATCAAAGTGGGCGATCAGCAGGTCGAAACCGTGTATATCCCGGACGGCGACCGTGCGACCCTGTGTGTCT
CTTCCCAGGTGGGTTGTGCACTGGAATGCAAATTCTGTTCGACCGCTCAGCAAGGCTTTAACCGCAACCTGCGCGTGTCG
GAAATCATTGGTCAGGTTTGGCGTGCGGCGAAAATTATCGGTGCGCTGAAAGTGACCGGCGAACGTCCGATCACCAACGT
GGTGATGATGGGCATGGGTGAGCCGTTGCTCAACCTGAACAACGTGGTACCGGCGATGGAAATCATGCTGGACGACTTCG
GTTTTGGCCTGTCCAAGCGTCGTGTGACCCTGTCTACTTCCGGCGTGGTGCCGGCACTGGACAAGTTGGGCGATATGATC
GACGTTGCACTGGCAATCTCGCTGCATGCGCCGAACGACAAGATCCGCGACGACATCGTGCCGATCAACCGTAAATACAA
TATCGAGACCTTCCTGGCGGCGGTACGTCGCTACCTGGCAAAATCCAATGCCAACCAGGGGCGGGTAACGGTTGAATACG
TGATGCTGGACCACATCAACGACAGCACTGACGACGCGCACCAGCTGGCAGAAGTCCTGAAGGACACGCCATGCAAGATC
AACCTGATCCCATGGAACCCGTTCCCGGGGGCACCTTACGGCCGAAGCTCCAACAGCCGGGTGGATCGTTTTTCCAAAGT
GTTGATGGAATACGGCTTTACGACTATTGTTCGTAAAACCCGTGGTGATGATATCGATGCCGCCTGCGGGCAACTGGCGG
GTGAAGTGATCGACCGTACCAAGCGTACCCTGAAAAAGAAAATGGCCGGGGAACCTATCTCTGTACGTGCGGTCTGA

Upstream 100 bases:

>100_bases
CGCTTTTAGCGCCGTCACCGCAATTTATGCGGGTTGAACGACGCACTTACTTTCCACTCTTTCGCGCCGTAACGTGTAAC
AACGAGGCCAGAGATCAATT

Downstream 100 bases:

>100_bases
ATCCTATAGCATGATTTTTTTGTTATCTTACAGTCTGTTATGTGAATGGACGTCAAGGTGACATCAGACAAGGAAATGAA
TGCAGGCATGAAGCTGACTC

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MLEPITSEHIVSENNSLTTQSVNAEQPAVAKINLLDLNRQQLREFFAKMGEKPFRADQVMKWMYHYCCDDFEQMTDINKV
LRNKLQSVAEIRAPEVAEEQRSADGTIKWAIKVGDQQVETVYIPDGDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVS
EIIGQVWRAAKIIGALKVTGERPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMI
DVALAISLHAPNDKIRDDIVPINRKYNIETFLAAVRRYLAKSNANQGRVTVEYVMLDHINDSTDDAHQLAEVLKDTPCKI
NLIPWNPFPGAPYGRSSNSRVDRFSKVLMEYGFTTIVRKTRGDDIDAACGQLAGEVIDRTKRTLKKKMAGEPISVRAV

Sequences:

>Translated_398_residues
MLEPITSEHIVSENNSLTTQSVNAEQPAVAKINLLDLNRQQLREFFAKMGEKPFRADQVMKWMYHYCCDDFEQMTDINKV
LRNKLQSVAEIRAPEVAEEQRSADGTIKWAIKVGDQQVETVYIPDGDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVS
EIIGQVWRAAKIIGALKVTGERPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMI
DVALAISLHAPNDKIRDDIVPINRKYNIETFLAAVRRYLAKSNANQGRVTVEYVMLDHINDSTDDAHQLAEVLKDTPCKI
NLIPWNPFPGAPYGRSSNSRVDRFSKVLMEYGFTTIVRKTRGDDIDAACGQLAGEVIDRTKRTLKKKMAGEPISVRAV
>Mature_398_residues
MLEPITSEHIVSENNSLTTQSVNAEQPAVAKINLLDLNRQQLREFFAKMGEKPFRADQVMKWMYHYCCDDFEQMTDINKV
LRNKLQSVAEIRAPEVAEEQRSADGTIKWAIKVGDQQVETVYIPDGDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVS
EIIGQVWRAAKIIGALKVTGERPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMI
DVALAISLHAPNDKIRDDIVPINRKYNIETFLAAVRRYLAKSNANQGRVTVEYVMLDHINDSTDDAHQLAEVLKDTPCKI
NLIPWNPFPGAPYGRSSNSRVDRFSKVLMEYGFTTIVRKTRGDDIDAACGQLAGEVIDRTKRTLKKKMAGEPISVRAV

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=392, Percent_Identity=85.2040816326531, Blast_Score=706, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_SERP5 (A8GHW8)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479836.1
- STRING:   A8GHW8
- GeneID:   5605865
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3612
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK11194
- BioCyc:   SPRO399741:SPRO_3612-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 44379; Mature: 44379

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEPITSEHIVSENNSLTTQSVNAEQPAVAKINLLDLNRQQLREFFAKMGEKPFRADQVM
CCCCCCCCHHCCCCCCCEEECCCCCCCCEEEEEEEECCHHHHHHHHHHHCCCCCCHHHHH
KWMYHYCCDDFEQMTDINKVLRNKLQSVAEIRAPEVAEEQRSADGTIKWAIKVGDQQVET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEEEECCCEEEE
VYIPDGDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIIGALKVTG
EEECCCCCEEEEEECCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEECC
ERPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMI
CCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHH
DVALAISLHAPNDKIRDDIVPINRKYNIETFLAAVRRYLAKSNANQGRVTVEYVMLDHIN
EEHHEEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCC
DSTDDAHQLAEVLKDTPCKINLIPWNPFPGAPYGRSSNSRVDRFSKVLMEYGFTTIVRKT
CCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHC
RGDDIDAACGQLAGEVIDRTKRTLKKKMAGEPISVRAV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MLEPITSEHIVSENNSLTTQSVNAEQPAVAKINLLDLNRQQLREFFAKMGEKPFRADQVM
CCCCCCCCHHCCCCCCCEEECCCCCCCCEEEEEEEECCHHHHHHHHHHHCCCCCCHHHHH
KWMYHYCCDDFEQMTDINKVLRNKLQSVAEIRAPEVAEEQRSADGTIKWAIKVGDQQVET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEEEECCCEEEE
VYIPDGDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIIGALKVTG
EEECCCCCEEEEEECCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEECC
ERPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMI
CCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHH
DVALAISLHAPNDKIRDDIVPINRKYNIETFLAAVRRYLAKSNANQGRVTVEYVMLDHIN
EEHHEEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCC
DSTDDAHQLAEVLKDTPCKINLIPWNPFPGAPYGRSSNSRVDRFSKVLMEYGFTTIVRKT
CCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHC
RGDDIDAACGQLAGEVIDRTKRTLKKKMAGEPISVRAV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA