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The map label for this gene is hisS

Identifier: 157371843

GI number: 157371843

Start: 3986929

End: 3988203

Strand: Reverse

Name: hisS

Synonym: Spro_3608

Alternate gene names: 157371843

Gene position: 3988203-3986929 (Counterclockwise)

Preceding gene: 157371844

Following gene: 157371842

Centisome position: 73.19

GC content: 58.82

Gene sequence:

>1275_bases
GTGGCAAAGAACATTCAAGCCATTCGCGGCATGAACGACTACCTGCCGGAAGAAACGGCATTATGGCAGCGTATTGAAGG
CATCCTCAAGCAGGTGCTTAGCGGCTACGGTTACAGCGAAATCCGCTTGCCGATTGTAGAGCAGACCCCGTTATTCAAAC
GCGCGATCGGCGAAGTGACCGACGTCGTGGAAAAAGAGATGTATACCTTCGACGACCGCAATGGCGAAAGCCTGACGCTG
CGTCCGGAAGGCACGGCTGGTTGCGTTCGCGCCGGCATCGAACATGGTCTGCTGTACAATCAGGAGCAGCGTCTGTGGTA
CGTCGGTCCGATGTTCCGCTACGAGCGCCCGCAAAAAGGCCGCTATCGCCAGTTCCATCAACTGGGGGCCGAAGTGTTTG
GCCTGCAGGGCCCGGATATTGACGCAGAGCTGATCCTGCTGAGCGCCCGCTGGTGGAAAGCGCTGGGGATTTCCGAGCAC
GTCAAGCTGGAGCTGAACTCCATCGGTTCACTGGAAGCGCGCGCCAACTACCGCGATGCTCTGGTGGCCTTCCTGGAACA
GCATCAAGAGAAGTTGGACGAAGATTGCAAACGCCGTATGTACAGCAACCCGCTGCGCGTACTGGACTCGAAAAACCCGG
AAGTGCAGGCGCTGTTGAACGATGCGCCACGCCTGTCCGAGTATCTGGATGCAGATTCCAAAGCCCACTTCGAAGGTCTG
TGTGAACTTTTGGCGCAGGCCGGCATCCCATATACCATCAATGAACGCCTGGTGCGCGGTCTGGACTACTACAACCGCAC
GGTATTCGAATGGGTAACCACCAGTCTGGGCGCTCAGGGCACGGTATGTGCCGGTGGTCGTTATGACGGCCTGGTAGAGC
AACTGGGCGGGCGTGCAACGCCGGCGGTCGGTTTCGCCATGGGCCTGGAGCGCCTGGTGTTGCTGGTTCAGGCGGTTAAC
CCAGAGTTCAAGGCCGCGGCGACCATCGACGTGTATGTGATCTCCTCCGGTGCCGGTACTCAGAGTGCAGCAATGCAGCT
GGCGGAGCGCGTGCGTGATGCGGCGCCACAGCTCAAACTGATGACCAACTACGGCGGTGGTAACTTTAAGAAGCAGATCA
CCCGTGCGGATAAGTGGGGCGCGCGCGTCGCCTTGATCCTGGGTGAGAACGAAGTCGCAGCCCAGCAGGTGGTGGTTAAG
GACCTGCGCAGTGGTGAACAAGAAACGCTGGCGCAAAGCGAAGTCGCAGTGCGCCTGGCTCTGATGTTAGGTTAA

Upstream 100 bases:

>100_bases
TTATGAACCCGGGAGCAGAACTCGGTATTCGCGTTGAATACGGGGCGGCAAAGTCCCTATAATCGGGTTCATTTTTTTAG
ACAACGGACAGAGAACTCAC

Downstream 100 bases:

>100_bases
GGAGAAGGACACCGTGGAAGTCTATACCACTGAAAACGAACAAGTCGACGCCGTGCGTCGGTTCTTTGCCGAAAATGGCA
AAGCGCTGGCAGTGGGCGTG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MAKNIQAIRGMNDYLPEETALWQRIEGILKQVLSGYGYSEIRLPIVEQTPLFKRAIGEVTDVVEKEMYTFDDRNGESLTL
RPEGTAGCVRAGIEHGLLYNQEQRLWYVGPMFRYERPQKGRYRQFHQLGAEVFGLQGPDIDAELILLSARWWKALGISEH
VKLELNSIGSLEARANYRDALVAFLEQHQEKLDEDCKRRMYSNPLRVLDSKNPEVQALLNDAPRLSEYLDADSKAHFEGL
CELLAQAGIPYTINERLVRGLDYYNRTVFEWVTTSLGAQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVN
PEFKAAATIDVYVISSGAGTQSAAMQLAERVRDAAPQLKLMTNYGGGNFKKQITRADKWGARVALILGENEVAAQQVVVK
DLRSGEQETLAQSEVAVRLALMLG

Sequences:

>Translated_424_residues
MAKNIQAIRGMNDYLPEETALWQRIEGILKQVLSGYGYSEIRLPIVEQTPLFKRAIGEVTDVVEKEMYTFDDRNGESLTL
RPEGTAGCVRAGIEHGLLYNQEQRLWYVGPMFRYERPQKGRYRQFHQLGAEVFGLQGPDIDAELILLSARWWKALGISEH
VKLELNSIGSLEARANYRDALVAFLEQHQEKLDEDCKRRMYSNPLRVLDSKNPEVQALLNDAPRLSEYLDADSKAHFEGL
CELLAQAGIPYTINERLVRGLDYYNRTVFEWVTTSLGAQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVN
PEFKAAATIDVYVISSGAGTQSAAMQLAERVRDAAPQLKLMTNYGGGNFKKQITRADKWGARVALILGENEVAAQQVVVK
DLRSGEQETLAQSEVAVRLALMLG
>Mature_423_residues
AKNIQAIRGMNDYLPEETALWQRIEGILKQVLSGYGYSEIRLPIVEQTPLFKRAIGEVTDVVEKEMYTFDDRNGESLTLR
PEGTAGCVRAGIEHGLLYNQEQRLWYVGPMFRYERPQKGRYRQFHQLGAEVFGLQGPDIDAELILLSARWWKALGISEHV
KLELNSIGSLEARANYRDALVAFLEQHQEKLDEDCKRRMYSNPLRVLDSKNPEVQALLNDAPRLSEYLDADSKAHFEGLC
ELLAQAGIPYTINERLVRGLDYYNRTVFEWVTTSLGAQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNP
EFKAAATIDVYVISSGAGTQSAAMQLAERVRDAAPQLKLMTNYGGGNFKKQITRADKWGARVALILGENEVAAQQVVVKD
LRSGEQETLAQSEVAVRLALMLG

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=444, Percent_Identity=25, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI15029520, Length=466, Percent_Identity=23.175965665236, Blast_Score=82, Evalue=9e-16,
Organism=Escherichia coli, GI1788861, Length=424, Percent_Identity=83.9622641509434, Blast_Score=749, Evalue=0.0,
Organism=Drosophila melanogaster, GI24643061, Length=483, Percent_Identity=23.8095238095238, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24643059, Length=498, Percent_Identity=23.8955823293173, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_SERP5 (A8GHW4)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479832.1
- ProteinModelPortal:   A8GHW4
- SMR:   A8GHW4
- STRING:   A8GHW4
- GeneID:   5605858
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3608
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- BioCyc:   SPRO399741:SPRO_3608-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47243; Mature: 47111

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNIQAIRGMNDYLPEETALWQRIEGILKQVLSGYGYSEIRLPIVEQTPLFKRAIGEVT
CCCCHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHH
DVVEKEMYTFDDRNGESLTLRPEGTAGCVRAGIEHGLLYNQEQRLWYVGPMFRYERPQKG
HHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHCCEECCCCCEEEECCHHHCCCCCCC
RYRQFHQLGAEVFGLQGPDIDAELILLSARWWKALGISEHVKLELNSIGSLEARANYRDA
HHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHCCCCCEEEEEECCCCCCHHHCCHHHH
LVAFLEQHQEKLDEDCKRRMYSNPLRVLDSKNPEVQALLNDAPRLSEYLDADSKAHFEGL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHCCCHHHHHHHCCCCCHHHHHH
CELLAQAGIPYTINERLVRGLDYYNRTVFEWVTTSLGAQGTVCAGGRYDGLVEQLGGRAT
HHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHCCCCC
PAVGFAMGLERLVLLVQAVNPEFKAAATIDVYVISSGAGTQSAAMQLAERVRDAAPQLKL
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEE
MTNYGGGNFKKQITRADKWGARVALILGENEVAAQQVVVKDLRSGEQETLAQSEVAVRLA
EECCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LMLG
HHCC
>Mature Secondary Structure 
AKNIQAIRGMNDYLPEETALWQRIEGILKQVLSGYGYSEIRLPIVEQTPLFKRAIGEVT
CCCHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHH
DVVEKEMYTFDDRNGESLTLRPEGTAGCVRAGIEHGLLYNQEQRLWYVGPMFRYERPQKG
HHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHCCEECCCCCEEEECCHHHCCCCCCC
RYRQFHQLGAEVFGLQGPDIDAELILLSARWWKALGISEHVKLELNSIGSLEARANYRDA
HHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHCCCCCEEEEEECCCCCCHHHCCHHHH
LVAFLEQHQEKLDEDCKRRMYSNPLRVLDSKNPEVQALLNDAPRLSEYLDADSKAHFEGL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHCCCHHHHHHHCCCCCHHHHHH
CELLAQAGIPYTINERLVRGLDYYNRTVFEWVTTSLGAQGTVCAGGRYDGLVEQLGGRAT
HHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHCCCCC
PAVGFAMGLERLVLLVQAVNPEFKAAATIDVYVISSGAGTQSAAMQLAERVRDAAPQLKL
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEE
MTNYGGGNFKKQITRADKWGARVALILGENEVAAQQVVVKDLRSGEQETLAQSEVAVRLA
EECCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LMLG
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA