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The map label for this gene is purR [H]

Identifier: 157371835

GI number: 157371835

Start: 3979698

End: 3980780

Strand: Direct

Name: purR [H]

Synonym: Spro_3600

Alternate gene names: 157371835

Gene position: 3979698-3980780 (Clockwise)

Preceding gene: 157371832

Following gene: 157371836

Centisome position: 73.04

GC content: 57.53

Gene sequence:

>1083_bases
ATGAACGGAAAGCTGAAAATCCAGGAGATCGCCCACCAAACCGGGTTGTCGATCAGCACCGTTTCACGCGTTTTAGCCGG
CAAGTCCAATACGAGCGTCGAAGCACGCCAAAAAGTGCTGGCCTGCGCCCAACAGAACGGCATCCTGCAGGGGATTTCCA
GCGGTCGACTGATGTTGAACAGCGTGATGGTCTTCGCGCCACAACGCGCTTTTGACGTACGTACCGATATTTTTTACTAC
AAGGTGATCCAGGGCATCACCGCTGCGCTGGCAGAACATGAGGTGCGTATTCGCTATTGCGGGCTTGAGGAACTGCACAG
TGACGGCGCGCTGTTTTTGGAAAAGATGAGCGATCCGCAGACCCAGGCGGCGCTGATTATCGGTATCGATGACGAGCATA
TCCACCAGTTAGCCGCAGATCTGAATAAACCCTGCGTGCTGATTAACTGCAGCGACCGGCAGATGCGGCTCGATAGCGTG
TCGCCCGATCATCAACTGATTGGCGAATTCTCCGCCAACTATCTGATCCAGCAGGGGCACAGCCATATTCTGAACCTGCA
GTGCCTGCGTCGGCACACTATGGAGCTGCGGCTGGCGGGCATCAAGCAAGCCTATGCGCGGCACAACATCGCCTTTGACG
AGGGTCGCCATCTGGTCACCACCTCCGGTTTCGGTAGCGAAGAGGCCGAACAGGCACTCACCACCTTTATCAACCGGGTC
AGTCAGCGCTCACAATTGCCGACGGCCATTCTTGCCGGTGGCGACTATATGGCGGTCGGCGCGGTCAAGGCACTCAACAA
ATTGCAGTTGAGCGTGCCAGGTGATATTTCGGTGATGAGCACCGACGGTTTTAATCTGGCGGAAATTCATGACGTGCCGC
TCACTTCGGTACACGTGCCGCGCGACGAGTTGGGCTATGAGGCCATTCAGCTACTGCAACGCCGCATGCTGCGCGCCGAT
GCGCCCCCGTGCAACCTGCTGCTGCATGGCAAGCTGGCGGTCCGCGCCTCGGTCAAACGCGTCAGCCCCCATAAAACCAG
CCCGGCGGTCAGCACTCACGATCACCGTCTTTACGATGTCTGA

Upstream 100 bases:

>100_bases
GAAGGATAAAAGCTCGCCATAATGGCCACCGAAGCCTGACAGTACGCTGAAAAAATGCAGTGAAAATATTTTCATCACCA
AATTTGAAGGAGATCACAGA

Downstream 100 bases:

>100_bases
ACTACACTTAGTGGCGGAATCTTTACTCAAGGAGAGCCACTATGCCGACCCTGACAGCGCGTTCGGTCATTCCCCCTTAT
ATGCTGCGTCGGATCATTGA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MNGKLKIQEIAHQTGLSISTVSRVLAGKSNTSVEARQKVLACAQQNGILQGISSGRLMLNSVMVFAPQRAFDVRTDIFYY
KVIQGITAALAEHEVRIRYCGLEELHSDGALFLEKMSDPQTQAALIIGIDDEHIHQLAADLNKPCVLINCSDRQMRLDSV
SPDHQLIGEFSANYLIQQGHSHILNLQCLRRHTMELRLAGIKQAYARHNIAFDEGRHLVTTSGFGSEEAEQALTTFINRV
SQRSQLPTAILAGGDYMAVGAVKALNKLQLSVPGDISVMSTDGFNLAEIHDVPLTSVHVPRDELGYEAIQLLQRRMLRAD
APPCNLLLHGKLAVRASVKRVSPHKTSPAVSTHDHRLYDV

Sequences:

>Translated_360_residues
MNGKLKIQEIAHQTGLSISTVSRVLAGKSNTSVEARQKVLACAQQNGILQGISSGRLMLNSVMVFAPQRAFDVRTDIFYY
KVIQGITAALAEHEVRIRYCGLEELHSDGALFLEKMSDPQTQAALIIGIDDEHIHQLAADLNKPCVLINCSDRQMRLDSV
SPDHQLIGEFSANYLIQQGHSHILNLQCLRRHTMELRLAGIKQAYARHNIAFDEGRHLVTTSGFGSEEAEQALTTFINRV
SQRSQLPTAILAGGDYMAVGAVKALNKLQLSVPGDISVMSTDGFNLAEIHDVPLTSVHVPRDELGYEAIQLLQRRMLRAD
APPCNLLLHGKLAVRASVKRVSPHKTSPAVSTHDHRLYDV
>Mature_360_residues
MNGKLKIQEIAHQTGLSISTVSRVLAGKSNTSVEARQKVLACAQQNGILQGISSGRLMLNSVMVFAPQRAFDVRTDIFYY
KVIQGITAALAEHEVRIRYCGLEELHSDGALFLEKMSDPQTQAALIIGIDDEHIHQLAADLNKPCVLINCSDRQMRLDSV
SPDHQLIGEFSANYLIQQGHSHILNLQCLRRHTMELRLAGIKQAYARHNIAFDEGRHLVTTSGFGSEEAEQALTTFINRV
SQRSQLPTAILAGGDYMAVGAVKALNKLQLSVPGDISVMSTDGFNLAEIHDVPLTSVHVPRDELGYEAIQLLQRRMLRAD
APPCNLLLHGKLAVRASVKRVSPHKTSPAVSTHDHRLYDV

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=339, Percent_Identity=26.5486725663717, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790194, Length=317, Percent_Identity=26.1829652996845, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787948, Length=197, Percent_Identity=29.4416243654822, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1789068, Length=351, Percent_Identity=26.7806267806268, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787580, Length=346, Percent_Identity=26.5895953757225, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786540, Length=362, Percent_Identity=25.6906077348066, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 39660; Mature: 39660

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGKLKIQEIAHQTGLSISTVSRVLAGKSNTSVEARQKVLACAQQNGILQGISSGRLMLN
CCCCEEHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHCCCCCHHEEH
SVMVFAPQRAFDVRTDIFYYKVIQGITAALAEHEVRIRYCGLEELHSDGALFLEKMSDPQ
HHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHCCCCHHHHHHCCCCC
TQAALIIGIDDEHIHQLAADLNKPCVLINCSDRQMRLDSVSPDHQLIGEFSANYLIQQGH
CCEEEEEECCHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHCCC
SHILNLQCLRRHTMELRLAGIKQAYARHNIAFDEGRHLVTTSGFGSEEAEQALTTFINRV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
SQRSQLPTAILAGGDYMAVGAVKALNKLQLSVPGDISVMSTDGFNLAEIHDVPLTSVHVP
HHHHCCCEEEEECCCEEEHHHHHHHHHEEEECCCCEEEEECCCCCEEEECCCCCCEEECC
RDELGYEAIQLLQRRMLRADAPPCNLLLHGKLAVRASVKRVSPHKTSPAVSTHDHRLYDV
HHHHHHHHHHHHHHHHHHCCCCCCCEEEECCEEEHHHHHHCCCCCCCCCCCCCCCEEECC
>Mature Secondary Structure
MNGKLKIQEIAHQTGLSISTVSRVLAGKSNTSVEARQKVLACAQQNGILQGISSGRLMLN
CCCCEEHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHCCCCCHHEEH
SVMVFAPQRAFDVRTDIFYYKVIQGITAALAEHEVRIRYCGLEELHSDGALFLEKMSDPQ
HHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHCCCCHHHHHHCCCCC
TQAALIIGIDDEHIHQLAADLNKPCVLINCSDRQMRLDSVSPDHQLIGEFSANYLIQQGH
CCEEEEEECCHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHCCC
SHILNLQCLRRHTMELRLAGIKQAYARHNIAFDEGRHLVTTSGFGSEEAEQALTTFINRV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
SQRSQLPTAILAGGDYMAVGAVKALNKLQLSVPGDISVMSTDGFNLAEIHDVPLTSVHVP
HHHHCCCEEEEECCCEEEHHHHHHHHHEEEECCCCEEEEECCCCCEEEECCCCCCEEECC
RDELGYEAIQLLQRRMLRADAPPCNLLLHGKLAVRASVKRVSPHKTSPAVSTHDHRLYDV
HHHHHHHHHHHHHHHHHHCCCCCCCEEEECCEEEHHHHHHCCCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA