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The map label for this gene is rluF [H]

Identifier: 157371821

GI number: 157371821

Start: 3960543

End: 3961343

Strand: Direct

Name: rluF [H]

Synonym: Spro_3586

Alternate gene names: 157371821

Gene position: 3960543-3961343 (Clockwise)

Preceding gene: 157371819

Following gene: 157371824

Centisome position: 72.69

GC content: 52.18

Gene sequence:

>801_bases
ATGCCAACTCAATCATCCATTCGCCTGAATAAATACATTAGCGACAGCGGTATCTGTTCCCGCCGTCAGGCCGATCGCTA
TATCAAACTGGGTAAGGTTTTTATCAACGATAACCAGGCCGAGGTGGGTGCGCCGGTATTTATTGGGGATGTTGTGAGTG
TTAACGGGCAACGGATAGAGCCGCGTAATGATGCAGATTTGGTGCTGATCGCGCTGAATAAACCCGTGGGCATCATCACC
AGCATGGGTAAACACGTGCCGAACAACATCGGTGATTTCGTTAACCACAGCCAGCGCACCTTCCCCGTCGGCCGCCTGGA
TAAAGACTCTCAGGGGCTGATTTTTCTGACCAACCGCGGTGAACTGGTCAATAAGATCCTGCGCGCCGGCAACAATCACC
AAAAAGAGTACCTGGTCACCGTCGACAAACCGCTGACCGATGAGTTTATTCAGGGCATGGGAGCGGGCGTGCCGATATTG
GGCAAAGTGACCCATAAATGCCAGGTGGAGCAAGAAGCTCCGCAGGTGTTACGCATTACGCTGGTGCAGGGGCTTAACCG
CCAAATCCGCCGTATGTGTAAATACTTTGGCTATCAAGTCACCAAACTTGAGCGCGTGCGCATTATGAACATCAGTCTGC
AAGGGTTAGCGCTGGGCGAATGGCGGGACTTGAGCGAGGATGAACGAACCGAATTATTTAAGCTGCTTGAGCACTCTTCG
TCCGAGGAGCCAGACCTGCCACAGAGAGACGAGATACCGCCAGGAAACAATAATCTGTTGCCCAACGATACCCCGTTATA
A

Upstream 100 bases:

>100_bases
CTATACTGCCATGAAAAACAGGCCTTTGACGATGTGCAATTTTAAGCAATCTTCGCAATACCCCCCGGGTAATGTATAGT
CCGCGTTTTACGGGGACACC

Downstream 100 bases:

>100_bases
CAAAACCGAATTTATCAAGAAATCAGAATGACCATCGGATGTTGGCATTAACGGCATTAACCTGGGTATCAGAACCGTAT
TGCCCCTGATAGCCGAGATC

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MPTQSSIRLNKYISDSGICSRRQADRYIKLGKVFINDNQAEVGAPVFIGDVVSVNGQRIEPRNDADLVLIALNKPVGIIT
SMGKHVPNNIGDFVNHSQRTFPVGRLDKDSQGLIFLTNRGELVNKILRAGNNHQKEYLVTVDKPLTDEFIQGMGAGVPIL
GKVTHKCQVEQEAPQVLRITLVQGLNRQIRRMCKYFGYQVTKLERVRIMNISLQGLALGEWRDLSEDERTELFKLLEHSS
SEEPDLPQRDEIPPGNNNLLPNDTPL

Sequences:

>Translated_266_residues
MPTQSSIRLNKYISDSGICSRRQADRYIKLGKVFINDNQAEVGAPVFIGDVVSVNGQRIEPRNDADLVLIALNKPVGIIT
SMGKHVPNNIGDFVNHSQRTFPVGRLDKDSQGLIFLTNRGELVNKILRAGNNHQKEYLVTVDKPLTDEFIQGMGAGVPIL
GKVTHKCQVEQEAPQVLRITLVQGLNRQIRRMCKYFGYQVTKLERVRIMNISLQGLALGEWRDLSEDERTELFKLLEHSS
SEEPDLPQRDEIPPGNNNLLPNDTPL
>Mature_265_residues
PTQSSIRLNKYISDSGICSRRQADRYIKLGKVFINDNQAEVGAPVFIGDVVSVNGQRIEPRNDADLVLIALNKPVGIITS
MGKHVPNNIGDFVNHSQRTFPVGRLDKDSQGLIFLTNRGELVNKILRAGNNHQKEYLVTVDKPLTDEFIQGMGAGVPILG
KVTHKCQVEQEAPQVLRITLVQGLNRQIRRMCKYFGYQVTKLERVRIMNISLQGLALGEWRDLSEDERTELFKLLEHSSS
EEPDLPQRDEIPPGNNNLLPNDTPL

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=242, Percent_Identity=71.4876033057851, Blast_Score=342, Evalue=1e-95,
Organism=Escherichia coli, GI1788510, Length=238, Percent_Identity=27.3109243697479, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1787524, Length=246, Percent_Identity=28.4552845528455, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI87081838, Length=194, Percent_Identity=29.3814432989691, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 29876; Mature: 29744

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTQSSIRLNKYISDSGICSRRQADRYIKLGKVFINDNQAEVGAPVFIGDVVSVNGQRIE
CCCCCCEEEEHHCCCCCCCCHHHHHHHEEEEEEEECCCCCCCCCCEEEECEEECCCCEEC
PRNDADLVLIALNKPVGIITSMGKHVPNNIGDFVNHSQRTFPVGRLDKDSQGLIFLTNRG
CCCCCCEEEEEECCCHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCC
ELVNKILRAGNNHQKEYLVTVDKPLTDEFIQGMGAGVPILGKVTHKCQVEQEAPQVLRIT
HHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHCCCCCCCHHHHHHHEECCHHHHHHHHHH
LVQGLNRQIRRMCKYFGYQVTKLERVRIMNISLQGLALGEWRDLSEDERTELFKLLEHSS
HHHHHHHHHHHHHHHHCCEEEEEEEEEEEEEEECEEEECCCCCCCHHHHHHHHHHHHCCC
SEEPDLPQRDEIPPGNNNLLPNDTPL
CCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PTQSSIRLNKYISDSGICSRRQADRYIKLGKVFINDNQAEVGAPVFIGDVVSVNGQRIE
CCCCCEEEEHHCCCCCCCCHHHHHHHEEEEEEEECCCCCCCCCCEEEECEEECCCCEEC
PRNDADLVLIALNKPVGIITSMGKHVPNNIGDFVNHSQRTFPVGRLDKDSQGLIFLTNRG
CCCCCCEEEEEECCCHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCC
ELVNKILRAGNNHQKEYLVTVDKPLTDEFIQGMGAGVPILGKVTHKCQVEQEAPQVLRIT
HHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHCCCCCCCHHHHHHHEECCHHHHHHHHHH
LVQGLNRQIRRMCKYFGYQVTKLERVRIMNISLQGLALGEWRDLSEDERTELFKLLEHSS
HHHHHHHHHHHHHHHHCCEEEEEEEEEEEEEEECEEEECCCCCCCHHHHHHHHHHHHCCC
SEEPDLPQRDEIPPGNNNLLPNDTPL
CCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]