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The map label for this gene is dapE

Identifier: 157371738

GI number: 157371738

Start: 3876960

End: 3878087

Strand: Direct

Name: dapE

Synonym: Spro_3501

Alternate gene names: 157371738

Gene position: 3876960-3878087 (Clockwise)

Preceding gene: 157371737

Following gene: 157371739

Centisome position: 71.15

GC content: 60.64

Gene sequence:

>1128_bases
ATGACTTGCCCGGTTATCGAACTGGCTCAACAGCTGATCAAGCGCCCGTCGCTCAGCCCGCACGATGAAGGCTGTCAGGC
ACTGATGATTGAACGTCTGGAGGCCATTGGTTTTACCGTTGAGCCGATGCCTTTCGGCGATACCCTGAATTTCTGGGCCT
GGCGTGGCGAAGGCCAAACCCTGGCGTTTGCCGGCCACACCGACGTCGTGCCAACCGGCGACGAAAAACGCTGGGACAAC
CCACCGTTTGAGCCGACCATCCGCGACGGCATGCTGTATGGCCGTGGCGCGGCAGACATGAAAGGCTCGCTGGCGGCAAT
GGTCGTGGCGGCCGAACGTTTCGTCGCGGCCAATCCGCATCATCAGGGCCGTTTGGCATTCCTGATCACCTCCGATGAAG
AAGCCAGCGCGACCCATGGCACGGTGAAAGTGGTGGAAGCGCTGATGGCGCGCAATGAGCGCCTGGATTATTGCCTGGTC
GGTGAGCCGTCCAGTACCGAACGCGTCGGCGACGTGGTGAAGAACGGGCGTCGCGGCTCAATCACCGCCAACCTGCACAT
TCACGGCATTCAGGGCCACGTTGCCTATCCACATCTGGCGGATAACCCGGTGCACCGCGCCATGCCGGCCCTCAATGAGC
TGGTGGCGATCGAGTGGGATCGCGGCAATGAATTCTTCCCGCCGACCAGCATGCAGATTGCTAACGTGCAGGCCGGTACC
GGCAGCAACAACGTGATCCCGGGCGAGATGTTCGTTCAGTTCAACTTCCGCTTTAGTACCGAATCGACCGACGCGACGAT
CAAACAGCGCGTAGAGGAACTGCTTGAGCGCCATCAGTTGAACTACAGCATCGAATGGCGCCTGTCGGGCCAGCCGTTTC
TGACTGCACGTGGCGCGCTGGTGGATGCGGTGGTTAACGCCGTCGAACACTACTCTGAGCTGACGCCGCAGTTGCTGACC
ACCGGGGGCACCTCCGACGGCCGCTTTATCGCCCAGATGGGCGCGCAGGTGGTTGAGCTGGGGCCAGTCAACGCGACTAT
CCATAAGGTCAACGAATGCGTACACGCGGCCGATTTGCAACTACTGAGCCGCATGTACCAGCGCATCATGGAGCAATTGG
TCGCATGA

Upstream 100 bases:

>100_bases
CGGCGATCATCAAACGCCCACTGCTGGACGACGGCAACGGGCACGCCCTGCTCGGCTTCAGCGCCGAAGGCTACCAGCAA
TTTATTTCCGAGGTGGCAGC

Downstream 100 bases:

>100_bases
TCACACCGGAAATGTTGACCGGCTGCTCAACCGAGCATCTGGCACCGCTGAGCGGTAACCACCGGCTGCAGCCCGCCGCG
GTGGCTGCATTCCTCGCCAT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MTCPVIELAQQLIKRPSLSPHDEGCQALMIERLEAIGFTVEPMPFGDTLNFWAWRGEGQTLAFAGHTDVVPTGDEKRWDN
PPFEPTIRDGMLYGRGAADMKGSLAAMVVAAERFVAANPHHQGRLAFLITSDEEASATHGTVKVVEALMARNERLDYCLV
GEPSSTERVGDVVKNGRRGSITANLHIHGIQGHVAYPHLADNPVHRAMPALNELVAIEWDRGNEFFPPTSMQIANVQAGT
GSNNVIPGEMFVQFNFRFSTESTDATIKQRVEELLERHQLNYSIEWRLSGQPFLTARGALVDAVVNAVEHYSELTPQLLT
TGGTSDGRFIAQMGAQVVELGPVNATIHKVNECVHAADLQLLSRMYQRIMEQLVA

Sequences:

>Translated_375_residues
MTCPVIELAQQLIKRPSLSPHDEGCQALMIERLEAIGFTVEPMPFGDTLNFWAWRGEGQTLAFAGHTDVVPTGDEKRWDN
PPFEPTIRDGMLYGRGAADMKGSLAAMVVAAERFVAANPHHQGRLAFLITSDEEASATHGTVKVVEALMARNERLDYCLV
GEPSSTERVGDVVKNGRRGSITANLHIHGIQGHVAYPHLADNPVHRAMPALNELVAIEWDRGNEFFPPTSMQIANVQAGT
GSNNVIPGEMFVQFNFRFSTESTDATIKQRVEELLERHQLNYSIEWRLSGQPFLTARGALVDAVVNAVEHYSELTPQLLT
TGGTSDGRFIAQMGAQVVELGPVNATIHKVNECVHAADLQLLSRMYQRIMEQLVA
>Mature_374_residues
TCPVIELAQQLIKRPSLSPHDEGCQALMIERLEAIGFTVEPMPFGDTLNFWAWRGEGQTLAFAGHTDVVPTGDEKRWDNP
PFEPTIRDGMLYGRGAADMKGSLAAMVVAAERFVAANPHHQGRLAFLITSDEEASATHGTVKVVEALMARNERLDYCLVG
EPSSTERVGDVVKNGRRGSITANLHIHGIQGHVAYPHLADNPVHRAMPALNELVAIEWDRGNEFFPPTSMQIANVQAGTG
SNNVIPGEMFVQFNFRFSTESTDATIKQRVEELLERHQLNYSIEWRLSGQPFLTARGALVDAVVNAVEHYSELTPQLLTT
GGTSDGRFIAQMGAQVVELGPVNATIHKVNECVHAADLQLLSRMYQRIMEQLVA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=375, Percent_Identity=84.8, Blast_Score=664, Evalue=0.0,
Organism=Escherichia coli, GI1790395, Length=259, Percent_Identity=30.1158301158301, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789236, Length=257, Percent_Identity=24.9027237354086, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_SERP5 (A8GHK9)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479727.1
- ProteinModelPortal:   A8GHK9
- SMR:   A8GHK9
- STRING:   A8GHK9
- GeneID:   5605029
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3501
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- ProtClustDB:   PRK13009
- BioCyc:   SPRO399741:SPRO_3501-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 41274; Mature: 41143

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2; PS00256 AKH

Important sites: ACT_SITE 68-68 ACT_SITE 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCPVIELAQQLIKRPSLSPHDEGCQALMIERLEAIGFTVEPMPFGDTLNFWAWRGEGQT
CCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEECCCCE
LAFAGHTDVVPTGDEKRWDNPPFEPTIRDGMLYGRGAADMKGSLAAMVVAAERFVAANPH
EEEECCCCCCCCCCCCCCCCCCCCCCHHCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCC
HQGRLAFLITSDEEASATHGTVKVVEALMARNERLDYCLVGEPSSTERVGDVVKNGRRGS
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCE
ITANLHIHGIQGHVAYPHLADNPVHRAMPALNELVAIEWDRGNEFFPPTSMQIANVQAGT
EEEEEEEEEECCCEECCCCCCCCHHHHHHHHHCEEEEEECCCCCCCCCCCCEEEEEECCC
GSNNVIPGEMFVQFNFRFSTESTDATIKQRVEELLERHQLNYSIEWRLSGQPFLTARGAL
CCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEECHHH
VDAVVNAVEHYSELTPQLLTTGGTSDGRFIAQMGAQVVELGPVNATIHKVNECVHAADLQ
HHHHHHHHHHHHHCCHHHEECCCCCCCCHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHH
LLSRMYQRIMEQLVA
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
TCPVIELAQQLIKRPSLSPHDEGCQALMIERLEAIGFTVEPMPFGDTLNFWAWRGEGQT
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEECCCCE
LAFAGHTDVVPTGDEKRWDNPPFEPTIRDGMLYGRGAADMKGSLAAMVVAAERFVAANPH
EEEECCCCCCCCCCCCCCCCCCCCCCHHCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCC
HQGRLAFLITSDEEASATHGTVKVVEALMARNERLDYCLVGEPSSTERVGDVVKNGRRGS
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCE
ITANLHIHGIQGHVAYPHLADNPVHRAMPALNELVAIEWDRGNEFFPPTSMQIANVQAGT
EEEEEEEEEECCCEECCCCCCCCHHHHHHHHHCEEEEEECCCCCCCCCCCCEEEEEECCC
GSNNVIPGEMFVQFNFRFSTESTDATIKQRVEELLERHQLNYSIEWRLSGQPFLTARGAL
CCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEECHHH
VDAVVNAVEHYSELTPQLLTTGGTSDGRFIAQMGAQVVELGPVNATIHKVNECVHAADLQ
HHHHHHHHHHHHHCCHHHEECCCCCCCCHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHH
LLSRMYQRIMEQLVA
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA