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The map label for this gene is cysK [H]

Identifier: 157371684

GI number: 157371684

Start: 3817793

End: 3818761

Strand: Direct

Name: cysK [H]

Synonym: Spro_3447

Alternate gene names: 157371684

Gene position: 3817793-3818761 (Clockwise)

Preceding gene: 157371683

Following gene: 157371685

Centisome position: 70.07

GC content: 57.69

Gene sequence:

>969_bases
ATGAGCAAGATATATGAAGACAACTCATTAACGATCGGCCATACGCCGCTGGTTCGTCTGAACCGTATCGGCAACGGACG
CATTCTGGCCAAGGTTGAATCACGCAACCCGAGCTTTAGCGTCAAGTGCCGCATCGGTGCCAATATGATTTGGGACGCAG
AAAAACGCGGCGTCCTGGTCGCCGGCAAAGAACTGGTGGAGCCAACCAGCGGCAACACCGGTATCGCACTGGCGTTCGTC
GCCGCCGCACGCGGCTATAAGCTGACGCTGACCATGCCGGAAACCATGAGCATCGAACGCCGCAAACTGCTCAAGGCGCT
GGGCGCCAACCTGGTGTTGACCGAAGGCGCTAAAGGCATGAAAGGCGCGATCGCCAAGGCGGAAGAAATTGTCGCCACCG
ATCCCAACCGCTACCTGATCCTGCAGCAGTTCAGCAACCCGGCCAACCCGGCGATCCACGAACAAACCACCGGCCCGGAA
ATCTGGGAAGATACCGATGGCGAAGTTGACGTTTTCATCTCTGGCGTCGGTACCGGCGGTACGCTGACCGGTGTCAGCCG
CTATATCAAGAACACCAAAGGCAAGGCGATTACCACCGTGGCGGTCGAACCTACCGACTCACCGGTGATCACCCAGGCGC
TGAACGGTGAAGAAATCAAACCGGGCCCGCACAAGATTCAGGGCATCGGCGCAGGTTTTATCCCTGGCAACCTGGATCTC
GACCTGGTTGACCGTGTCGAGCAAATCACCAACGACGAAGCCATCAGCATGGCACGCCGCCTGATGGACGAAGAAGGGAT
TCTGGCAGGCATTTCCTCCGGGGCCGCCGTAGCCGCAGCGGTGAAACTGGCTGAAGAACCAGAGTTCGCCGACAAAACCA
TCGTGGTGATATTACCTTCCTCCGGCGAGCGCTATTTGAGCACCGCCTTGTTCGCCGATTTGTTCACCGAACAAGAATTG
CAACAGTAG

Upstream 100 bases:

>100_bases
TAATAACATATAGATATACCAATTCTTTACTTCCCTGCCGGTACTGTTAGCGTATGCTTTCGACGTTCCCCACATTTTTC
ATAGAGTTAAAGGACGGGCT

Downstream 100 bases:

>100_bases
TGCCAGCCGCGCACTAATCGCTAAAAAAGCACCTTACGGGGTGCTTTTTTGTGGACTGGATCAAAGTTTATAGCAGTCGA
AGATTGATTTTACCCGCCAG

Product: cysteine synthase A

Products: NA

Alternate protein names: CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; Sulfate starvation-induced protein 5; SSI5 [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSKIYEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCRIGANMIWDAEKRGVLVAGKELVEPTSGNTGIALAFV
AAARGYKLTLTMPETMSIERRKLLKALGANLVLTEGAKGMKGAIAKAEEIVATDPNRYLILQQFSNPANPAIHEQTTGPE
IWEDTDGEVDVFISGVGTGGTLTGVSRYIKNTKGKAITTVAVEPTDSPVITQALNGEEIKPGPHKIQGIGAGFIPGNLDL
DLVDRVEQITNDEAISMARRLMDEEGILAGISSGAAVAAAVKLAEEPEFADKTIVVILPSSGERYLSTALFADLFTEQEL
QQ

Sequences:

>Translated_322_residues
MSKIYEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCRIGANMIWDAEKRGVLVAGKELVEPTSGNTGIALAFV
AAARGYKLTLTMPETMSIERRKLLKALGANLVLTEGAKGMKGAIAKAEEIVATDPNRYLILQQFSNPANPAIHEQTTGPE
IWEDTDGEVDVFISGVGTGGTLTGVSRYIKNTKGKAITTVAVEPTDSPVITQALNGEEIKPGPHKIQGIGAGFIPGNLDL
DLVDRVEQITNDEAISMARRLMDEEGILAGISSGAAVAAAVKLAEEPEFADKTIVVILPSSGERYLSTALFADLFTEQEL
QQ
>Mature_321_residues
SKIYEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCRIGANMIWDAEKRGVLVAGKELVEPTSGNTGIALAFVA
AARGYKLTLTMPETMSIERRKLLKALGANLVLTEGAKGMKGAIAKAEEIVATDPNRYLILQQFSNPANPAIHEQTTGPEI
WEDTDGEVDVFISGVGTGGTLTGVSRYIKNTKGKAITTVAVEPTDSPVITQALNGEEIKPGPHKIQGIGAGFIPGNLDLD
LVDRVEQITNDEAISMARRLMDEEGILAGISSGAAVAAAVKLAEEPEFADKTIVVILPSSGERYLSTALFADLFTEQELQ
Q

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=325, Percent_Identity=39.3846153846154, Blast_Score=203, Evalue=1e-52,
Organism=Homo sapiens, GI295821200, Length=325, Percent_Identity=39.3846153846154, Blast_Score=203, Evalue=1e-52,
Organism=Homo sapiens, GI4557415, Length=325, Percent_Identity=39.3846153846154, Blast_Score=203, Evalue=1e-52,
Organism=Escherichia coli, GI1788754, Length=323, Percent_Identity=87.6160990712074, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI2367138, Length=311, Percent_Identity=42.443729903537, Blast_Score=219, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17535051, Length=307, Percent_Identity=54.7231270358306, Blast_Score=303, Evalue=8e-83,
Organism=Caenorhabditis elegans, GI17562970, Length=318, Percent_Identity=51.2578616352201, Blast_Score=294, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI32566674, Length=307, Percent_Identity=52.1172638436482, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI115535073, Length=318, Percent_Identity=52.5157232704402, Blast_Score=280, Evalue=6e-76,
Organism=Caenorhabditis elegans, GI17561720, Length=316, Percent_Identity=50.9493670886076, Blast_Score=263, Evalue=8e-71,
Organism=Caenorhabditis elegans, GI32566672, Length=226, Percent_Identity=50.4424778761062, Blast_Score=218, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI17534315, Length=322, Percent_Identity=38.5093167701863, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI25147552, Length=322, Percent_Identity=35.7142857142857, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17561716, Length=131, Percent_Identity=47.3282442748092, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6321594, Length=313, Percent_Identity=36.741214057508, Blast_Score=172, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6321449, Length=335, Percent_Identity=32.2388059701493, Blast_Score=126, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24643623, Length=315, Percent_Identity=38.4126984126984, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI20129101, Length=315, Percent_Identity=38.4126984126984, Blast_Score=163, Evalue=2e-40,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34382; Mature: 34251

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIYEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCRIGANMIWDAEKRGVLV
CCCEECCCCEEECCCCEEEEEECCCCEEEEEEECCCCCEEEEEEECCCEEECCCCCCEEE
AGKELVEPTSGNTGIALAFVAAARGYKLTLTMPETMSIERRKLLKALGANLVLTEGAKGM
ECHHHCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCC
KGAIAKAEEIVATDPNRYLILQQFSNPANPAIHEQTTGPEIWEDTDGEVDVFISGVGTGG
HHHHHHHHHEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
TLTGVSRYIKNTKGKAITTVAVEPTDSPVITQALNGEEIKPGPHKIQGIGAGFIPGNLDL
HHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCH
DLVDRVEQITNDEAISMARRLMDEEGILAGISSGAAVAAAVKLAEEPEFADKTIVVILPS
HHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCCEEEEEECC
SGERYLSTALFADLFTEQELQQ
CCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKIYEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCRIGANMIWDAEKRGVLV
CCEECCCCEEECCCCEEEEEECCCCEEEEEEECCCCCEEEEEEECCCEEECCCCCCEEE
AGKELVEPTSGNTGIALAFVAAARGYKLTLTMPETMSIERRKLLKALGANLVLTEGAKGM
ECHHHCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCC
KGAIAKAEEIVATDPNRYLILQQFSNPANPAIHEQTTGPEIWEDTDGEVDVFISGVGTGG
HHHHHHHHHEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
TLTGVSRYIKNTKGKAITTVAVEPTDSPVITQALNGEEIKPGPHKIQGIGAGFIPGNLDL
HHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCH
DLVDRVEQITNDEAISMARRLMDEEGILAGISSGAAVAAAVKLAEEPEFADKTIVVILPS
HHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCCEEEEEECC
SGERYLSTALFADLFTEQELQQ
CCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]