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The map label for this gene is nupC [H]

Identifier: 157371653

GI number: 157371653

Start: 3778158

End: 3779345

Strand: Direct

Name: nupC [H]

Synonym: Spro_3416

Alternate gene names: 157371653

Gene position: 3778158-3779345 (Clockwise)

Preceding gene: 157371651

Following gene: 157371659

Centisome position: 69.34

GC content: 51.01

Gene sequence:

>1188_bases
ATGTCCCATATTGCGCACTTCGCGTTGGCGTTGGTGGTGGTCACGATCCTGGCACTTTTAGTATGCCGCGATCGTAAAAG
CATCCGCATTCGCTACGTTATTCAACTGTTGGTTATTGAAGTACTGCTGGCCTATTTTTTCCTGCATTCGGAAGCGGGTT
TAGGCTTTGTAAAAGGATTCGCAGCGCTGTTTGACAAGCTGCTCGGGTTTGCTGGGCAAGGGACTGAGTTTGTATTCGGC
GGTATGGGCGACAAAGGTTTGGCCTTCTTCTTCCTGAAGGTACTGTGCCCGATCGTCTTCATCTCCGCGCTGATCGGTAT
TCTGCAATACATTAAAGTACTGCCGTTTGTCATCCGCATCATTGGTACCGTGCTGTCAAAAGTTAACGGTATGGGCAAGC
TGGAATCGTTCAACGCCGTCAGCTCGCTGATCCTGGGCCAGTCTGAAAACTTCATTGCCTATAAAGATATTCTGGGCAAG
ATGTCTGAAAAGCGCATGTACACCATGGCGGCGACCGCGATGTCTACGGTTTCCATGTCCATCGTCGGCGCTTACATGAC
CATGCTGGACGCCAAATTTGTGGTCGCGGCACTGGTACTGAACATGTTCAGCACCTTTATCGTGCTGTCATTGGTCAACC
CGTATGACACCAGTAAAGAAGAAGAACTGCACCTGGGCAATTTGCACGAAGGCCAGAGCTTCTTCGAAATGCTGGGTGAA
TACATTCTGGCTGGTTTTAAAGTAGCTATTATCGTTGCTGCGATGCTGATCGGCTTTATCGCCCTGATCGCCGCGCTCAA
CGGCCTGTTCAGCGCCATCTTCGGCCTGAGCTTCCAGGAAATTCTGGGCTACTTCTTCTATCCATTCGCCTGGATTATGG
GTATTCCTAAGCACGAAGCGTTGCAGGTGGGTAGCATCATGGCAACCAAACTGGTATCCAACGAATTCGTGGCGATGATG
GAACTGCAGAAAATCTCTGCTGAACTCTCTCCTCGCAGCATGGGTATTCTGTCGGTGTTCCTGGTTTCCTTCGCCAACTT
CTCTTCCATCGGCATCGTTGCCGGTGCGATTAAAGGGCTGAACGAGCATCAGGGCAACGTGGTTTCTCGCTTTGGTCTGA
AGCTGGTTTACGGTTCAACGCTGGTCAGCATCCTGTCTGCGTCCATCGCAGGCCTGGTTCTGGGCTAA

Upstream 100 bases:

>100_bases
CAGTATGAATTTCCATATTTGCAATAGACCTCTCGTTTTTAAACGTGGCGTTACATAAAATGACATCCGAAATTCAGCCT
GCCTCAAGTTTGGAGCAAAC

Downstream 100 bases:

>100_bases
GCCGAAACGTCAGTAAGATATCAGTAAGATAAAGGTCGCTTCGGCGGCCTTTTTTAATGCCTGCAATTCGGTGCATGGGA
AAGGGAAGGCAGTAAGGAAA

Product: nucleoside transporter

Products: uridine [Cytoplasm]; thymidine [Cytoplasm]; inosine [Cytoplasm]; Deoxyuridine [Cytoplasm]; deoxyinosine [Cytoplasm]; deoxycytidine [Cytoplasm]; deoxyadenosine [Cytoplasm]; cytidine [Cytoplasm]; Proton [Cytoplasm]; adenosine [Cytoplasm] [C]

Alternate protein names: Nucleoside-transport system protein nupC [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MSHIAHFALALVVVTILALLVCRDRKSIRIRYVIQLLVIEVLLAYFFLHSEAGLGFVKGFAALFDKLLGFAGQGTEFVFG
GMGDKGLAFFFLKVLCPIVFISALIGILQYIKVLPFVIRIIGTVLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
MSEKRMYTMAATAMSTVSMSIVGAYMTMLDAKFVVAALVLNMFSTFIVLSLVNPYDTSKEEELHLGNLHEGQSFFEMLGE
YILAGFKVAIIVAAMLIGFIALIAALNGLFSAIFGLSFQEILGYFFYPFAWIMGIPKHEALQVGSIMATKLVSNEFVAMM
ELQKISAELSPRSMGILSVFLVSFANFSSIGIVAGAIKGLNEHQGNVVSRFGLKLVYGSTLVSILSASIAGLVLG

Sequences:

>Translated_395_residues
MSHIAHFALALVVVTILALLVCRDRKSIRIRYVIQLLVIEVLLAYFFLHSEAGLGFVKGFAALFDKLLGFAGQGTEFVFG
GMGDKGLAFFFLKVLCPIVFISALIGILQYIKVLPFVIRIIGTVLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
MSEKRMYTMAATAMSTVSMSIVGAYMTMLDAKFVVAALVLNMFSTFIVLSLVNPYDTSKEEELHLGNLHEGQSFFEMLGE
YILAGFKVAIIVAAMLIGFIALIAALNGLFSAIFGLSFQEILGYFFYPFAWIMGIPKHEALQVGSIMATKLVSNEFVAMM
ELQKISAELSPRSMGILSVFLVSFANFSSIGIVAGAIKGLNEHQGNVVSRFGLKLVYGSTLVSILSASIAGLVLG
>Mature_394_residues
SHIAHFALALVVVTILALLVCRDRKSIRIRYVIQLLVIEVLLAYFFLHSEAGLGFVKGFAALFDKLLGFAGQGTEFVFGG
MGDKGLAFFFLKVLCPIVFISALIGILQYIKVLPFVIRIIGTVLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGKM
SEKRMYTMAATAMSTVSMSIVGAYMTMLDAKFVVAALVLNMFSTFIVLSLVNPYDTSKEEELHLGNLHEGQSFFEMLGEY
ILAGFKVAIIVAAMLIGFIALIAALNGLFSAIFGLSFQEILGYFFYPFAWIMGIPKHEALQVGSIMATKLVSNEFVAMME
LQKISAELSPRSMGILSVFLVSFANFSSIGIVAGAIKGLNEHQGNVVSRFGLKLVYGSTLVSILSASIAGLVLG

Specific function: Transports nucleosides with a high affinity except guanosine and deoxyguanosine. Driven by a proton motive force [H]

COG id: COG1972

COG function: function code F; Nucleoside permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family [H]

Homologues:

Organism=Homo sapiens, GI42542381, Length=352, Percent_Identity=27.5568181818182, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI11545853, Length=354, Percent_Identity=25.1412429378531, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI227116277, Length=405, Percent_Identity=24.6913580246914, Blast_Score=99, Evalue=8e-21,
Organism=Escherichia coli, GI1788737, Length=400, Percent_Identity=80.5, Blast_Score=633, Evalue=0.0,
Organism=Escherichia coli, GI1788485, Length=409, Percent_Identity=29.5843520782396, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1788488, Length=407, Percent_Identity=29.7297297297297, Blast_Score=159, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17560276, Length=392, Percent_Identity=26.530612244898, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71991794, Length=390, Percent_Identity=24.3589743589744, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI45552517, Length=385, Percent_Identity=27.2727272727273, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19921868, Length=385, Percent_Identity=27.2727272727273, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI45552519, Length=385, Percent_Identity=27.2727272727273, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI281360430, Length=351, Percent_Identity=25.3561253561254, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008276
- InterPro:   IPR018270
- InterPro:   IPR011657
- InterPro:   IPR002668 [H]

Pfam domain/function: PF07662 Nucleos_tra2_C; PF01773 Nucleos_tra2_N [H]

EC number: NA

Molecular weight: Translated: 42829; Mature: 42698

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHIAHFALALVVVTILALLVCRDRKSIRIRYVIQLLVIEVLLAYFFLHSEAGLGFVKGF
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AALFDKLLGFAGQGTEFVFGGMGDKGLAFFFLKVLCPIVFISALIGILQYIKVLPFVIRI
HHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGTVLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGKMSEKRMYTMAATAMSTVSMS
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVGAYMTMLDAKFVVAALVLNMFSTFIVLSLVNPYDTSKEEELHLGNLHEGQSFFEMLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
YILAGFKVAIIVAAMLIGFIALIAALNGLFSAIFGLSFQEILGYFFYPFAWIMGIPKHEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHH
LQVGSIMATKLVSNEFVAMMELQKISAELSPRSMGILSVFLVSFANFSSIGIVAGAIKGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
NEHQGNVVSRFGLKLVYGSTLVSILSASIAGLVLG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SHIAHFALALVVVTILALLVCRDRKSIRIRYVIQLLVIEVLLAYFFLHSEAGLGFVKGF
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AALFDKLLGFAGQGTEFVFGGMGDKGLAFFFLKVLCPIVFISALIGILQYIKVLPFVIRI
HHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGTVLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGKMSEKRMYTMAATAMSTVSMS
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVGAYMTMLDAKFVVAALVLNMFSTFIVLSLVNPYDTSKEEELHLGNLHEGQSFFEMLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
YILAGFKVAIIVAAMLIGFIALIAALNGLFSAIFGLSFQEILGYFFYPFAWIMGIPKHEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHH
LQVGSIMATKLVSNEFVAMMELQKISAELSPRSMGILSVFLVSFANFSSIGIVAGAIKGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
NEHQGNVVSRFGLKLVYGSTLVSILSASIAGLVLG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine [Periplasm]; thymidine [Periplasm]; inosine [Periplasm]; Deoxyuridine [Periplasm]; deoxyinosine [Periplasm]; deoxycytidine [Periplasm]; deoxyadenosine [Periplasm]; cytidine [Periplasm]; Proton [Periplasm]; adenosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uridine [Periplasm] = Proton [Cytoplasm] + uridine [Cytoplasm] Proton [Periplasm] + thymidine [Periplasm] = Proton [Cytoplasm] + thymidine [Cytoplasm] Proton [Periplasm] + inosine [Periplasm] = Proton [Cytoplasm] + inosine [Cytoplasm]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]